Abstract:
:The biosynthesis of specific polypeptides directed by purified viral messenger RNA from JLS-V9 cells infected with Rauscher leukemia virus has been studied in a rabbit reticulocyte lysate. The 35S viral mRNA gives rise to two major products of 65,000 and 72,000 molecular weight. The synthesis of specific polypeptides was also investigated in lysates derived from infected cells. The main products were polypeptides with molecular weights of 65,000, 76,000, and 82,000, and were preferentially made in association with membranes. The relative content of the virus-specific polypeptide of 65,000 molecular weight, synthesized in a cell-free system supplemented with purified polyribosomes, is considerably higher for membrane-bound polyribosomes.
journal_name
Proc Natl Acad Sci U S Aauthors
Gielkens AL,Van Zaane D,Bloemers HP,Bloemendal Hdoi
10.1073/pnas.73.2.356keywords:
subject
Has Abstractpub_date
1976-02-01 00:00:00pages
356-60issue
2eissn
0027-8424issn
1091-6490journal_volume
73pub_type
杂志文章abstract::We propose that protein translocation across membranes is driven by biased random thermal motion. This "Brownian ratchet" mechanism depends on chemical asymmetries between the cis and trans sides of the membrane. Several mechanisms could contribute to rectifying the thermal motion of the protein, such as binding and d...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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abstract::Peptides from the three type I repeats of human endothelial cell thrombospondin, containing the consensus sequence-Trp-Ser-Xaa-Trp-, bind to sulfated glycoconjugates including heparin and sulfatide. The peptides are potent inhibitors for the binding of thrombospondin, laminin, or apolipoprotein E to these ligands. The...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2013-07-02 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2009-03-03 00:00:00
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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abstract::When assembled into 13--19S particles, the reovirus nonstructural protein sigma-NS selectively binds single-stranded RNAs. Sedimentation analyses combined with binding to nitrocellulose membrane filters showed that 1--2 pmol of reovirus mRNAs from the large, medium, or small size classes saturated in vitro the binding...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:1982-06-01 00:00:00
abstract::We have asked here how the remarkable variation in maize haplotype structure affects recombination. We compared recombination across a genetic interval of 9S in 2 highly dissimilar heterozygotes that shared 1 parent. The genetic interval in the common haplotype is approximately 100 kb long and contains 6 genes intersp...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2009-05-26 00:00:00
abstract::Recent research has emphasized the importance of the metabolic cluster, which includes glucose intolerance, dyslipidemia, and high blood pressure, as a strong predictor of the obesity-related morbidities and premature mortality. Fundamental to this association, commonly referred to as the metabolic syndrome, is the cl...
journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2000-12-19 00:00:00