Abstract:
:Cells in the brain act as components of extended networks. Therefore, to understand neurobiological processes in a physiological context, it is essential to study them in vivo. Super-resolution microscopy has spatial resolution beyond the diffraction limit, thus promising to provide structural and functional insights that are not accessible with conventional microscopy. However, to apply it to in vivo brain imaging, we must address the challenges of 3D imaging in an optically heterogeneous tissue that is constantly in motion. We optimized image acquisition and reconstruction to combat sample motion and applied adaptive optics to correcting sample-induced optical aberrations in super-resolution structured illumination microscopy (SIM) in vivo. We imaged the brains of live zebrafish larvae and mice and observed the dynamics of dendrites and dendritic spines at nanoscale resolution.
journal_name
Proc Natl Acad Sci U S Aauthors
Turcotte R,Liang Y,Tanimoto M,Zhang Q,Li Z,Koyama M,Betzig E,Ji Ndoi
10.1073/pnas.1819965116subject
Has Abstractpub_date
2019-05-07 00:00:00pages
9586-9591issue
19eissn
0027-8424issn
1091-6490pii
1819965116journal_volume
116pub_type
杂志文章abstract::A small proportion of the RNAs of mouse reticulocytes consists of beta major-globin mRNA sequences linked to sequences transcribed from the 5'-flanking region of the beta major-globin gene. These upstream RNAs are polyadenylylated and contain 700-800 nucleotides, and their 5' regions are heterogeneous. RNAs with simil...
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