Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos.

abstract：:Rare nucleated fetal cells circulate within maternal blood. Noninvasive prenatal diagnosis by isolation and genetic analysis of these cells is currently being undertaken. We sought to determine if genetic evidence existed for persistent circulation of fetal cells from prior pregnancies. Venous blood samples were obtai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bianchi DW,Zickwolf GK,Weil GJ,Sylvester S,DeMaria MA

更新日期：1996-01-23 00:00:00

abstract：:Since the end of World War II, much evidence has accrued of the primary phase of village-farming community life in Southwestern Asia, which began about 7000 B.C. The remains of (usually) several of the positively domesticated animals (dog, sheep, goat, pig) and plants (wheat, barley, legumes such as peas and lentils) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braidwood RJ,Cambel H,Redman CL,Watson PJ

更新日期：1971-06-01 00:00:00

abstract：:Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheong CG,Hall TM

更新日期：2006-09-12 00:00:00

abstract：:An ADP analog carrying a biotin moiety and a photoreactive group was synthesized. In the presence of vanadate ion (Vi), the analog was tightly trapped into the ATPase site of heavy meromyosin (HMM) or myosin subfragment 1 (S1) in an ADP analog/ATPase site molar ratio of 1:1. UV illumination on the HMM (or S1)-Vi-ADP a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sutoh K,Yamamoto K,Wakabayashi T

更新日期：1986-01-01 00:00:00

abstract：:The kinetics for hydrogen exchange at individual base pairs in self-complementary deoxydodecanucleotide duplexes have been estimated from NMR saturation recovery measurements on the resolved imino protons as a function of temperature. The imino protons of dA . dT base pairs in the center of the fully alternating d(C-G...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel DJ,Ikuta S,Kozlowski S,Itakura K

更新日期：1983-04-01 00:00:00

abstract：:The human tumor necrosis factor-alpha (TNF-alpha) gene encodes a pleiotropic cytokine that plays a critical role in basic immunologic processes. To investigate the TNF-alpha regulatory region in the primate lineage, we isolated TNF-alpha promoters from representative great apes, Old World monkeys, and New World monkey...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung JY,McKenzie FE,Uglialoro AM,Flores-Villanueva PO,Sorkin BC,Yunis EJ,Hartl DL,Goldfeld AE

更新日期：2000-06-06 00:00:00

abstract：:Carrying premature termination codons in 1 allele of the ABCA7 gene is associated with an increased risk for Alzheimer's disease (AD). While the primary function of ABCA7 is to regulate the transport of phospholipids and cholesterol, ABCA7 is also involved in maintaining homeostasis of the immune system. Since inflamm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aikawa T,Ren Y,Yamazaki Y,Tachibana M,Johnson MR,Anderson CT,Martens YA,Holm ML,Asmann YW,Saito T,Saido TC,Fitzgerald ML,Bu G,Kanekiyo T

更新日期：2019-11-19 00:00:00

abstract：:Physical and gene mapping studies reveal that chloroplast DNA from geranium (Pelargonium hortorum) has sustained a number of extensive duplications and inversions, resulting in a genome arrangement radically unlike that of other plants. At 217 kilobases in size, the circular chromosome is about 50% larger than the typ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palmer JD,Nugent JM,Herbon LA

更新日期：1987-02-01 00:00:00

abstract：:RNA interference (RNAi) is triggered in eukaryotic organisms by double-stranded RNA (dsRNA), and it destroys any mRNA that has sequence identity with the dsRNA trigger. The RNAi pathway in Anopheles gambiae can be silenced by transfecting cells with dsRNA derived from exon sequence of the A. gambiae Argonaute2 (AgAgo2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keene KM,Foy BD,Sanchez-Vargas I,Beaty BJ,Blair CD,Olson KE

更新日期：2004-12-07 00:00:00

abstract：:The plant trans-Golgi network (TGN) is a central trafficking hub where secretory, vacuolar, recycling, and endocytic pathways merge. Among currently known molecular players involved in TGN transport, three different adaptor protein (AP) complexes promote vesicle generation at the TGN with different cargo specificity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heinze L,Freimuth N,Rößling AK,Hahnke R,Riebschläger S,Fröhlich A,Sampathkumar A,McFarlane HE,Sauer M

更新日期：2020-10-13 00:00:00

abstract：:Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction of tissue with ATP at low ionic strength and coprecipitation with actin, followed by gel filtration on Sepharose 4B, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barylko B,Wagner MC,Reizes O,Albanesi JP

更新日期：1992-01-15 00:00:00

abstract：:The Fas/APO-1 cytotoxic pathway plays an important role in the regulation of peripheral immunity. Recent evidence indicates that this regulatory function operates through deletion of activated T and B lymphocytes by CD4+ T cells expressing the Fas ligand. Because macrophages play a key role in peripheral immunity, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ashany D,Song X,Lacy E,Nikolic-Zugic J,Friedman SM,Elkon KB

更新日期：1995-11-21 00:00:00

abstract：:Besides ethanol, acetic acid is produced in naturally fermenting sweet resources and is a significant environmental stress for fruit-breeding Drosophila populations and species. Although not related to the presence of an active alcohol dehydrogenase, adult acetic acid tolerance was found to correlate with ethanol tole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chakir M,Peridy O,Capy P,Pla E,David JR

更新日期：1993-04-15 00:00:00

abstract：:Erythropoietin regulates the proliferation and differentiation of erythroid precursor cells. Its effect is mediated by the erythropoietin receptor (EPOR), a member of a large family of cytokine receptors. The EPOR gene has recently been cloned, sequenced, and characterized. As shown experimentally, its intracellular C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de la Chapelle A,Träskelin AL,Juvonen E

更新日期：1993-05-15 00:00:00

abstract：:The functional consequences of interrupting in utero binocular interactions were studied by recording from single cells in area 17 of adult cats that had one eye removed at least 2 wk before birth. In these animals all cortical neurons could be driven by the remaining eye, and in tangential microelectrode penetrations...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shook BL,Maffei L,Chalupa LM

更新日期：1985-06-01 00:00:00

abstract：:Hybridization in plants leads to transinteractions between the parental genomes and epigenomes that can result in changes to both 24 nt siRNA and cytosine methylation ((m)C) levels in the hybrid. In Arabidopsis the principle processes altering the hybrid methylome are Trans Chromosomal Methylation (TCM) and Trans Chro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greaves IK,Groszmann M,Wang A,Peacock WJ,Dennis ES

更新日期：2014-02-04 00:00:00

abstract：:Members of the SLC4 bicarbonate transporter family are involved in solute transport and pH homeostasis. Here we report that disrupting the Slc4a10 gene, which encodes the Na(+)-coupled Cl(-)-HCO(3)(-) exchanger Slc4a10 (NCBE), drastically reduces brain ventricle volume and protects against fatal epileptic seizures in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacobs S,Ruusuvuori E,Sipilä ST,Haapanen A,Damkier HH,Kurth I,Hentschke M,Schweizer M,Rudhard Y,Laatikainen LM,Tyynelä J,Praetorius J,Voipio J,Hübner CA

更新日期：2008-01-08 00:00:00

abstract：:As the Conus shell grows by spiraling of the outer lip around the axis, profound internal shell dissolution thins the walls of the protected penultimate whorl from several millimeters to <50mum. Shell material is added to the inside of the spire and the anterior part of the columella. The resulting shell has a uniform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kohn AJ,Myers ER,Meenakshi VR

更新日期：1979-07-01 00:00:00

abstract：:Clonal expansion of premalignant lesions is an important step in the progression to cancer. This process is commonly considered to be a consequence of sustaining a proliferative mutation. Here, we investigate whether the growth trajectory of clones can be better described by a model in which clone growth does not depe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chao DL,Eck JT,Brash DE,Maley CC,Luebeck EG

更新日期：2008-09-30 00:00:00

abstract：:To study the structure and function of the gene for parathyroid hormone, we obtained recombinant plasmids containing bovine parathyroid hormone cDNA. The nucleotide sequence at the 5' terminus (relative to the sense strand) of the cDNA insert in a recombinant plasmid, pPTHi4, was different from that previously reporte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weaver CA,Gordon DF,Kemper B

更新日期：1981-07-01 00:00:00

abstract：:The virions of vesicular stomatitis virus contain an enzyme that catalyzes the incorporation of ribonucleotides into RNA. The product of the reaction is mainly RNA complementary in base sequence to that of vesicular stomatitis virus RNA. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baltimore D,Huang AS,Stampfer M

更新日期：1970-06-01 00:00:00

abstract：:The superparamagnetic behavior of very small particles of metallic iron (ca 1.5 nm), with about half of their atoms at the surface, is changed reversibly by adsorption and desorption of hydrogen below the superparamagnetic transition temperature. The change after adsorption implies a lowering of the anisotropy energy ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boudart M,Dumesic JA,Topsøe H

更新日期：1977-03-01 00:00:00

abstract：:Suppressor T cells have been shown to be much more radiosensitive than other lymphoïd cells, and we have tried to reduce tumor growth by low-dose irradiation. Syngeneic DBA/2 mice received whole-body irradiation (150 rads; 1 rad = 0.01 J/kg) 6 days after P815 tumor inoculation. Tumor growth is significantly reduced in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tilkin AF,Schaaf-Lafontaine N,Van Acker A,Boccadoro M,Urbain J

更新日期：1981-03-01 00:00:00

abstract：:Using chimeric recombinants transfected into HeLa cells and a transient expression assay, we demonstrate that the 5'-flanking region of the pS2 gene from position -428 to position -324 exhibits both constitutive and estrogen-inducible enhancer activity. The estrogen-inducible activity, but not the constitutive activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berry M,Nunez AM,Chambon P

更新日期：1989-02-01 00:00:00

abstract：:Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Martinez D,Challacombe J,Morgenstern I,Hibbett D,Schmoll M,Kubicek CP,Ferreira P,Ruiz-Duenas FJ,Martinez AT,Kersten P,Hammel KE,Vanden Wymelenberg A,Gaskell J,Lindquist E,Sabat G,Bondurant SS,Larrondo LF,Canessa P,Vic

更新日期：2009-02-10 00:00:00

abstract：:Mutant I1A cells, lacking IL-1 receptor-associated kinase (IRAK) mRNA and protein, have been used to study the involvement of IRAK in NFkappaB and c-Jun N-terminal kinase (JNK) activation. A series of IRAK deletion constructs were expressed in I1A cells, which were then tested for their ability to respond to IL-1. Bot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li X,Commane M,Jiang Z,Stark GR

更新日期：2001-04-10 00:00:00

abstract：:Previous studies have shown that defective virus vectors (amplicons) derived from herpes simplex viruses could be efficiently propagated in virus stocks in the presence of trans-acting helper virus functions. The present study established that two separate cis-acting functions--a DNA replication origin and a cleavage/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spaete RR,Frenkel N

更新日期：1985-02-01 00:00:00

abstract：:A discussion of the expected mechanism of radical reduction of carbonyl compounds by dihydroflavin as it relates to the structure of the carbonyl compound is provided. Factors which must be taken into account are the free energies of formation of the radical anions (CR2O), THE ACIDITY OF THE CONJUGATE ACID OF THE CARB...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams RF,Shinkai S,Bruice TC

更新日期：1975-05-01 00:00:00

abstract：:The c-ras family is a set of c-onc genes that are highly conserved in vertebrates. The genes in this family are homologous to the transforming genes of Harvey and Kirsten murine sarcoma viruses (v-Ha-ras and v-Ki-ras, respectively). Using an in situ molecular hybridization method, we detected three sites on the human ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jhanwar SC,Neel BG,Hayward WS,Chaganti RS

更新日期：1983-08-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize self lipid antigens presented by CD1d molecules. The nature of the self-antigens involved in the development and maturation of iNKT cells is poorly defined. Lysophospholipids are self-antigens presented by CD1d that are generated through the action of phospholipases A1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paduraru C,Bezbradica JS,Kunte A,Kelly R,Shayman JA,Veerapen N,Cox LR,Besra GS,Cresswell P

更新日期：2013-03-26 00:00:00