当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Purification and characterization of a mammalian myosin I.

Purification and characterization of a mammalian myosin I.

Abstract：

:Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction of tissue with ATP at low ionic strength and coprecipitation with actin, followed by gel filtration on Sepharose 4B, anion-exchange chromatography on Q Sepharose, and affinity chromatography on ATP-agarose. Mammalian myosin I molecules are composed of a heavy chain of 116 kDa and multiple low molecular weight polypeptides identified as calmodulin. The structural and enzymatic properties of adrenal medulla myosin I were further characterized. This enzyme exhibits high K+,EDTA- and Ca(2+)-ATPase specific activities (about 0.2 mumol.min-1 per mg of protein), whereas the Mg(2+)-ATPase activity is very low (1-3 nmol.min-1.mg-1). The Mg(2+)-ATPase of medulla myosin I is activated by F-actin in a Ca(2+)-dependent manner: activity is stimulated 40-fold in the presence of EGTA and 90-fold in the presence of 10 microM Ca2+. Two structural domains of the myosin I heavy chain were identified. A 74-kDa chymotryptic fragment contains the catalytic site, while a 36-kDa polypeptide contains the calmodulin-binding sites. These results indicate that mammalian myosin I is more closely related to myosin I from the avian intestinal brush border than to the enzymes isolated from the protozoans Acanthamoeba and Dictyostelium.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Barylko B,Wagner MC,Reizes O,Albanesi JP

doi

10.1073/pnas.89.2.490

keywords:

subject

Has Abstract

pub_date

1992-01-15 00:00:00

pages

490-4

issue

2

eissn

0027-8424

issn

1091-6490

journal_volume

89

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Inhibition of homologous recombination by variants of the catalytic subunit of the DNA-dependent protein kinase (DNA-PKcs).

    abstract：:Two major DNA double-strand break repair pathways exist in all eukaryotes, nonhomologous DNA end joining (NHEJ) and homologous recombination (HR). Although both pathways can function throughout the cell cycle, NHEJ predominates in G0/G1) (when a replicated sister chromatid is unavailable), whereas HR makes a more subs...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0406466102

    authors： Convery E,Shin EK,Ding Q,Wang W,Douglas P,Davis LS,Nickoloff JA,Lees-Miller SP,Meek K

    更新日期：2005-02-01 00:00:00

  • Tumor necrosis factor alpha induces expression of human immunodeficiency virus in a chronically infected T-cell clone.

    abstract：:Tumor necrosis factor alpha (TNF-alpha), also known as cachectin, was demonstrated to induce the expression of human immunodeficiency virus (HIV) in a chronically infected T-cell clone (ACH-2). Concentrations of recombinant TNF-alpha as low as 50 pg/ml induced a significant increase over background of HIV expression i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.7.2365

    authors： Folks TM,Clouse KA,Justement J,Rabson A,Duh E,Kehrl JH,Fauci AS

    更新日期：1989-04-01 00:00:00

  • Expression cloning of rat renal Na+/SO4(2-) cotransport.

    abstract：:Injection of rat kidney cortex mRNA into Xenopus laevis oocytes leads to a stimulation of Na(+)-dependent SO4(2-) uptake. Based on this information, we have isolated from a corresponding library a cDNA (NaSi-1) that is most likely related to a Na+/SO4(2-) cotransport system. NaSi-1 cRNA leads in a time- and dose-depen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.17.8073

    authors： Markovich D,Forgo J,Stange G,Biber J,Murer H

    更新日期：1993-09-01 00:00:00

  • Monoclonal antibodies to native noncollagenous bone-specific proteins.

    abstract：:Hybridoma technology was used for preparation of murine monoclonal antibodies of high titer against bone-Gla protein and osteonectin. A procedure of immunization and hybridization similar to that already described [Katzmann, J.A., Nesheim, M.E., Hibbard, L.S. & Mann, K.G. (1981) Proc. Natl. Acad. Sci. USA 78, 162-166;...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.9.2868

    authors： Stenner DD,Romberg RW,Tracy RP,Katzmann JA,Riggs BL,Mann KG

    更新日期：1984-05-01 00:00:00

  • Spectral discrimination in color blind animals via chromatic aberration and pupil shape.

    abstract：:We present a mechanism by which organisms with only a single photoreceptor, which have a monochromatic view of the world, can achieve color discrimination. An off-axis pupil and the principle of chromatic aberration (where different wavelengths come to focus at different distances behind a lens) can combine to provide...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1524578113

    authors： Stubbs AL,Stubbs CW

    更新日期：2016-07-19 00:00:00

  • Mating-type switching by chromosomal inversion in methylotrophic yeasts suggests an origin for the three-locus Saccharomyces cerevisiae system.

    abstract：:Saccharomyces cerevisiae has a complex system for switching the mating type of haploid cells, requiring the genome to have three mating-type (MAT)-like loci and a mechanism for silencing two of them. How this system originated is unknown, because the three-locus system is present throughout the family Saccharomycetace...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1416014111

    authors： Hanson SJ,Byrne KP,Wolfe KH

    更新日期：2014-11-11 00:00:00

  • A general genetic approach in Escherichia coli for determining the mechanism(s) of action of tumoricidal agents: application to DMP 840, a tumoricidal agent.

    abstract：:We describe here a simple and easily manipulatable Escherichia coli-based genetic system that permits us to identify bacterial gene products that modulate the sensitivity of bacteria to tumoricidal agents, such as DMP 840, a bisnaphthalimide drug. To the extent that the action of these agents is conserved, these studi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.19.8950

    authors： Chatterjee PK,Sternberg NL

    更新日期：1995-09-12 00:00:00

  • Activation of integrin beta-subunit I-like domains by one-turn C-terminal alpha-helix deletions.

    abstract：:Integrins contain two structurally homologous but distantly related domains: an I-like domain that is present in all beta-subunits and an I domain that is present in some alpha-subunits. Atomic resolution and mutagenesis studies of alpha I domains demonstrate a C-terminal, axial displacement of the alpha7-helix that a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0307291101

    authors： Yang W,Shimaoka M,Chen J,Springer TA

    更新日期：2004-02-24 00:00:00

  • Primate lentiviral virion infectivity factors are substrate receptors that assemble with cullin 5-E3 ligase through a HCCH motif to suppress APOBEC3G.

    abstract：:Cullin-Ring E3 ubiquitin ligases target substrates for ubiquitin-dependent, proteasome-mediated degradation and regulate critical cellular processes. These cullins assemble with cellular substrate receptor proteins through specific adaptor molecules. F-box- and BC-box-containing receptors use Skp1, ElonginB, and Elong...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0502440102

    authors： Luo K,Xiao Z,Ehrlich E,Yu Y,Liu B,Zheng S,Yu XF

    更新日期：2005-08-09 00:00:00

  • Target neuron-specific process formation by embryonic mesencephalic dopamine neurons in vitro.

    abstract：:Mesencephalic dopamine neurons from the embryonic mouse brain were dissociated, aggregated in vitro in the presence of dissociated cells from appropriate or inappropriate target neuron areas, and visualized by the Falck-Hillarp histofluorescence technique after exposure to 1 microM exogenous dopamine. When aggregated ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.2.1264

    authors： Hemmendinger LM,Garber BB,Hoffmann PC,Heller A

    更新日期：1981-02-01 00:00:00

  • Identification of GLUT12/SLC2A12 as a urate transporter that regulates the blood urate level in hyperuricemia model mice.

    abstract：:Recent genome-wide association studies have revealed some genetic loci associated with serum uric acid levels and susceptibility to gout/hyperuricemia which contain potential candidates of physiologically important urate transporters. One of these novel loci is located upstream of SGK1 and SLC2A12, suggesting that var...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2006958117

    authors： Toyoda Y,Takada T,Miyata H,Matsuo H,Kassai H,Nakao K,Nakatochi M,Kawamura Y,Shimizu S,Shinomiya N,Ichida K,Hosoyamada M,Aiba A,Suzuki H

    更新日期：2020-08-04 00:00:00

  • Identification of Id4 as a regulator of BRCA1 expression by using a ribozyme-library-based inverse genomics approach.

    abstract：:Expression of the breast and ovarian cancer susceptibility gene BRCA1 is down-regulated in sporadic breast and ovarian cancer cases. Therefore, the identification of genes involved in the regulation of BRCA1 expression might lead to new insights into the pathogenesis and treatment of these tumors. In the present study...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.98.1.130

    authors： Beger C,Pierce LN,Kruger M,Marcusson EG,Robbins JM,Welcsh P,Welch PJ,Welte K,King MC,Barber JR,Wong-Staal F

    更新日期：2001-01-02 00:00:00

  • Asian inland wildfires driven by glacial-interglacial climate change.

    abstract：:Wildfire can influence climate directly and indirectly, but little is known about the relationships between wildfire and climate during the Quaternary, especially how wildfire patterns varied over glacial-interglacial cycles. Here, we present a high-resolution soot record from the Chinese Loess Plateau; this is a reco...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1822035117

    authors： Han Y,An Z,Marlon JR,Bradley RS,Zhan C,Arimoto R,Sun Y,Zhou W,Wu F,Wang Q,Burr GS,Cao J

    更新日期：2020-03-10 00:00:00

  • Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis.

    abstract：:FIP-3 (14.7K interacting protein) was discovered during a search for cell proteins that could interact with an adenovirus protein (Ad E3-14.7K) that had been shown to prevent tumor necrosis factor (TNF)-alpha-induced cytolysis. FIP-3, which contains leucine zippers and a zinc finger domain, inhibits both basal and ind...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.3.1042

    authors： Li Y,Kang J,Friedman J,Tarassishin L,Ye J,Kovalenko A,Wallach D,Horwitz MS

    更新日期：1999-02-02 00:00:00

  • Visualizing the dynamics of T cell activation: intracellular adhesion molecule 1 migrates rapidly to the T cell/B cell interface and acts to sustain calcium levels.

    abstract：:T cell recognition typically involves both the engagement of a specific T cell receptor with a peptide/major histocompatibility complex (MHC) and a number of accessory interactions. One of the most important interactions is between the integrin lymphocyte function-associated antigen 1 (LFA-1) on the T cell and intrace...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.6302

    authors： Wülfing C,Sjaastad MD,Davis MM

    更新日期：1998-05-26 00:00:00

  • Transient inositol 1,4,5-trisphosphate-induced Ca2+ release: a model based on regulatory Ca(2+)-binding sites along the permeation pathway.

    abstract：:A remarkable property of Ca2+ fluxes through the inositol 1,4,5-trisphosphate (InsP3)-gated Ca2+ channel is that successive increments of InsP3 induce repeated transient release of accumulated Ca2+. The initial aim of this study was to propose a model, based on hypotheses compatible with the current description of thi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.21.10074

    authors： Swillens S,Combettes L,Champeil P

    更新日期：1994-10-11 00:00:00

  • Molecular and biological changes in the cold-adapted "master strain" A/AA/6/60 (H2N2) influenza virus.

    abstract：:The live cold-adapted (ca) A/AA/6/60 influenza vaccine is being commercially developed for worldwide use in children and is being used as a model for other live vaccines. Although it has been proven safe and immunogenic, the molecular basis of cold adaptation has never been determined. To identify sequence changes res...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.13.6032

    authors： Herlocher ML,Maassab HF,Webster RG

    更新日期：1993-07-01 00:00:00

  • Selective cleavage of kinetoplast DNA minicircles promoted by antitrypanosomal drugs.

    abstract：:Pentamidine, diminazene aceturate (Berenil), isometamidium chloride (Samorin), and ethidium bromide, which are important antitrypanosomal drugs, promote linearization of Trypanosoma equiperdum minicircle DNA (the principal component of kinetoplast DNA, the mitochondrial DNA in these parasites). This effect occurs at t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.3.950

    authors： Shapiro TA,Englund PT

    更新日期：1990-02-01 00:00:00

  • Release of 1-O-alkylglyceryl 3-phosphorylcholine, O-deacetyl platelet-activating factor, from leukocytes: chemical ionization mass spectrometry of phospholipids.

    abstract：:Evidence is presented for the simultaneous release of platelet-activating factor (PAF-acether) and of its deacetylated derivative (lyso-PAF-acether) from hog leukocytes. On the basis of spectroscopy and chemical reactions, the structure of O-deacetyl-PAF is shown to be 1-O-alkylglyceryl 3-phosphorylcholine, an alkyl e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.12.7019

    authors： Polonsky J,Tencé M,Varenne P,Das BC,Lunel J,Benveniste J

    更新日期：1980-12-01 00:00:00

  • ATP: The crucial component of secretory vesicles.

    abstract：:The colligative properties of ATP and catecholamines demonstrated in vitro are thought to be responsible for the extraordinary accumulation of solutes inside chromaffin cell secretory vesicles, although this has yet to be demonstrated in living cells. Because functional cells cannot be deprived of ATP, we have knocked...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1600690113

    authors： Estévez-Herrera J,Domínguez N,Pardo MR,González-Santana A,Westhead EW,Borges R,Machado JD

    更新日期：2016-07-12 00:00:00

  • Tight clustering and hemizygosity of apomixis-linked molecular markers in Pennisetum squamulatum implies genetic control of apospory by a divergent locus that may have no allelic form in sexual genotypes.

    abstract：:Apomixis is a naturally occurring mode of reproduction that results in embryo formation without the involvement of meiosis or fertilization of the egg. Seed-derived progeny of an apomictic plant are genetically identical to the maternal parent. We are studying a form of apomixis called apospory that occurs in the genu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.9.5127

    authors： Ozias-Akins P,Roche D,Hanna WW

    更新日期：1998-04-28 00:00:00

  • Cardiac myosin-binding protein C decorates F-actin: implications for cardiac function.

    abstract：:Cardiac myosin-binding protein C (cMyBP-C) is an accessory protein of striated muscle sarcomeres that is vital for maintaining regular heart function. Its 4 N-terminal regulatory domains, C0-C1-m-C2 (C0C2), influence actin and myosin interactions, the basic contractile proteins of muscle. Using neutron contrast variat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0808903105

    authors： Whitten AE,Jeffries CM,Harris SP,Trewhella J

    更新日期：2008-11-25 00:00:00

  • Manganese and zinc blockade of enzyme induction: studies with microsomal heme oxygenase.

    abstract：:Heme oxygenase (decyclizing) [heme,hydrogen-donor:oxygen oxidoreductase (alpha-methene-oxidizing, hydroxylating), EC 1.14.99.3] is greatly induced in the kidney by the administration of nickel or tin. Manganese, when administered simultaneously with nickel or tin in an equimolar amount, substantially inhibited the ind...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.10.5331

    authors： Drummond GS,Kappas A

    更新日期：1979-10-01 00:00:00

  • Stimulation of Ebola virus production from persistent infection through activation of the Ras/MAPK pathway.

    abstract：:Human infections with Ebola virus (EBOV) result in a deadly viral disease known as Ebola hemorrhagic fever. Up to 90% of infected patients die, and there is no available treatment or vaccine. The sporadic human outbreaks are believed to result when EBOV "jumps" from an infected animal to a person and is subsequently t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0809698105

    authors： Strong JE,Wong G,Jones SE,Grolla A,Theriault S,Kobinger GP,Feldmann H

    更新日期：2008-11-18 00:00:00

  • The O-fucose glycan in the ligand-binding domain of Notch1 regulates embryogenesis and T cell development.

    abstract：:Mechanisms by which the extracellular domain of Notch1 controls Notch1 signaling are not well defined. Here, we show that the O-fucose glycan in the Notch1 ligand-binding domain regulates the strength of Notch1 signaling during embryogenesis, postweaning growth, and T cell development in the mouse. Heterozygotes carry...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702846105

    authors： Ge C,Stanley P

    更新日期：2008-02-05 00:00:00

  • Ecological and socioeconomic effects of China's policies for ecosystem services.

    abstract：:To address devastating environmental crises and to improve human well-being, China has been implementing a number of national policies on payments for ecosystem services. Two of them, the Natural Forest Conservation Program (NFCP) and the Grain to Green Program (GTGP), are among the biggest programs in the world becau...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,评审

    doi：10.1073/pnas.0706436105

    authors： Liu J,Li S,Ouyang Z,Tam C,Chen X

    更新日期：2008-07-15 00:00:00

  • Extent of Fermi-surface reconstruction in the high-temperature superconductor HgBa2CuO4+δ.

    abstract：:High magnetic fields have revealed a surprisingly small Fermi surface in underdoped cuprates, possibly resulting from Fermi-surface reconstruction due to an order parameter that breaks translational symmetry of the crystal lattice. A crucial issue concerns the doping extent of such a state and its relationship to the ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1914166117

    authors： Chan MK,McDonald RD,Ramshaw BJ,Betts JB,Shekhter A,Bauer ED,Harrison N

    更新日期：2020-05-05 00:00:00

  • Akt2 overexpression plays a critical role in the establishment of colorectal cancer metastasis.

    abstract：:Colorectal cancer is the second leading cause of cancer-related deaths in the United States. Understanding the distinct genetic and epigenetic changes contributing to the establishment and growth of metastatic lesions is crucial for the development of novel therapeutic strategies. In a search for key regulators of col...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0810715105

    authors： Rychahou PG,Kang J,Gulhati P,Doan HQ,Chen LA,Xiao SY,Chung DH,Evers BM

    更新日期：2008-12-23 00:00:00

  • A highly conserved sequence is a novel gene involved in de novo vitamin B6 biosynthesis.

    abstract：:The Cercospora nicotianae SOR1 (singlet oxygen resistance) gene was identified previously as a gene involved in resistance of this fungus to singlet-oxygen-generating phototoxins. Although homologues to SOR1 occur in organisms in four kingdoms and encode one of the most highly conserved proteins yet identified, the pr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.16.9374

    authors： Ehrenshaft M,Bilski P,Li MY,Chignell CF,Daub ME

    更新日期：1999-08-03 00:00:00

  • The thermal impulse response of Escherichia coli.

    abstract：:Swimming Escherichia coli responds to changes in temperature by modifying its motor behavior. Previous studies using populations of cells have shown that E. coli accumulate in spatial thermal gradients, but these experiments did not cleanly separate thermal responses from chemotactic responses. Here we have isolated t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0709903105

    authors： Paster E,Ryu WS

    更新日期：2008-04-08 00:00:00