The thermal impulse response of Escherichia coli.

abstract：:Knowledge of normal human cardiac excitation stems from isolated heart or intraoperative mapping studies under nonphysiological conditions. Here, we use a noninvasive imaging modality (electrocardiographic imaging) to study normal activation and repolarization in intact unanesthetized healthy adults under complete phy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramanathan C,Jia P,Ghanem R,Ryu K,Rudy Y

更新日期：2006-04-18 00:00:00

abstract：:Elucidating the mechanism of folding of polynucleotides depends on accurate estimates of free energy surfaces and a quantitative description of the kinetics of structure formation. Here, the kinetics of hairpin formation in single-stranded DNA are measured after a laser temperature jump. The kinetics are modeled as co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ansari A,Kuznetsov SV,Shen Y

更新日期：2001-07-03 00:00:00

abstract：:Human diploid fibroblasts underwent lysis when resuspended in isotonic sucrose. After preparation of a granular fraction by differential centrifugation, lysosomes were almost completely purified by free-flow electrophoresis. Electron micrographs of the final fraction of fibroblast lysosomes (about 25-fold purified) sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harms E,Kern H,Schneider JA

更新日期：1980-10-01 00:00:00

abstract：:Protein misfolding and the formation of aggregates are increasingly recognized components of the pathology of human genetic disease and hallmarks of many neurodegenerative disorders. As exemplified by polyglutamine diseases, the propensity for protein misfolding is associated with the length of polyglutamine expansion...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nollen EA,Garcia SM,van Haaften G,Kim S,Chavez A,Morimoto RI,Plasterk RH

更新日期：2004-04-27 00:00:00

abstract：:Spatial working memory or short-term place memory is impaired in schizophrenia. The efficiency of antipsychotic drugs, particularly of typical antipsychotics, on cognitive deficit in schizophrenia remains disputable. Inhibition of serotonin (5-HT) 2A/2C receptors is important for cognitive improvement in schizophrenic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bubenikova-Valesova V,Stuchlik A,Svoboda J,Bures J,Vales K

更新日期：2008-01-22 00:00:00

abstract：:Myosin VI is a molecular motor implicated in many processes, and it likely associates with a variety of cargoes that specify its functions. Although it is critical to Drosophila development, little is known about its cellular roles. To reveal its involvement in specific pathways, we sought to identify the binding part...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finan D,Hartman MA,Spudich JA

更新日期：2011-04-05 00:00:00

abstract：:Emerging evidence suggests that transforming growth factor-beta (TGF-beta) is an important mediator of diabetic nephropathy. We showed previously that short-term treatment with a neutralizing monoclonal anti-TGF-beta antibody (alphaT) in streptozotocin-diabetic mice prevents early changes of renal hypertrophy and incr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ziyadeh FN,Hoffman BB,Han DC,Iglesias-De La Cruz MC,Hong SW,Isono M,Chen S,McGowan TA,Sharma K

更新日期：2000-07-05 00:00:00

abstract：:Poliovirus RNA polymerase requires a host factor to initiate RNA synthesis in vitro. The host factor was previously purified to near homogeneity from HeLa cells but was not assigned an enzymatic activity. This report describes the purification of a terminal uridylyltransferase that can act as host factor. By all crite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andrews NC,Baltimore D

更新日期：1986-01-01 00:00:00

abstract：:Clones of embryonic chick chondrocytes have been isolated and collagen biosynthesis has been followed as the clones grow and eventually lose division capacity. Analysis of collagen type at each successive subculture until the time of cellular senescence has shown that a change in synthesis occurs from the cartilage-sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayne R,Vail MS,Mayne PM,Miller EJ

更新日期：1976-05-01 00:00:00

abstract：:Bacillus thuringiensis subsp. kurstaki produces a proteinaceous crystalline inclusion that is toxic for lepidopteran larvae. There are several size classes of plasmids in this organism and the presence of one or more has been correlated with production of this protein, defined as a protoxin. DNA fragments of B. thurin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Held GA,Bulla LA Jr,Ferrari E,Hoch J,Aronson AI,Minnich SA

更新日期：1982-10-01 00:00:00

abstract：:Phytochrome A (phyA) is the only plant photoreceptor that perceives far-red light and then mediates various responses to this signal. Phosphorylation and dephosphorylation of oat phyA have been extensively studied, and it was shown that phosphorylation of a serine residue in the hinge region of oat phyA could regulate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou Y,Yang L,Duan J,Cheng J,Shen Y,Wang X,Han R,Li H,Li Z,Wang L,Terzaghi W,Zhu D,Chen H,Deng XW,Li J

更新日期：2018-12-11 00:00:00

abstract：:We previously have shown that proliferating lymphoid cells contain an extractable cytoplasmic protein that is capable of inducing DNA synthesis in isolated quiescent nuclei (activator of DNA replication, ADR). ADR is present in continuously proliferating (transformed) lymphoblastoid cell lines and in mitogen- or inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gutowski JK,West A,Cohen S

更新日期：1985-08-01 00:00:00

abstract：:The neotropical Heliconius butterflies are famous examples of Müllerian mimicry, due to the diverse array of shared, brightly colored wing patterns that advertise the butterflies' unpalatability. The parallel geographical variation in these patterns within several widespread species has been invoked to support the con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brower AV

更新日期：1994-07-05 00:00:00

abstract：:We have examined the functional consequences of mutations present in defective alleles of the retinoblastoma susceptibility gene (RB1) isolated from two spontaneously arising tumors. Unlike cDNA clones expressing the wild-type protein p110Rb, those encoding the two mutant proteins failed to induce the appearance of se...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Templeton DJ,Park SH,Lanier L,Weinberg RA

更新日期：1991-04-15 00:00:00

abstract：:Tight junctions (TJs) play a key role in mediating paracellular ion reabsorption in the kidney. The paracellular pathway in the collecting duct of the kidney is a predominant route for transepithelial chloride reabsorption that determines the extracellular NaCl content and the blood pressure. However, the molecular me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou J,Renigunta A,Yang J,Waldegger S

更新日期：2010-10-19 00:00:00

abstract：:Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemical synthesis of si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu JY,DeRuiter SL,Turner DL

更新日期：2002-04-30 00:00:00

abstract：:Interest in cell-associated collagens led others to isolate A and B collagen chains, also known as type V collagen, from many tissues, but only after pepsin cleavage. Soluble precursors of these chains are synthesized in vitro by crop, a chicken embryo muscle tissue, and are converted by at least two processing steps ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumamoto CA,Fessler JH

更新日期：1980-11-01 00:00:00

abstract：:The growth or decay of population of a single species interacting with a large number of other species (or environment) according to the Volterra-Lotka model is investigated. When the environment is initially very close to its equilibrium level, the growth of a single species follows a generalized Verhulst law, contai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zwanzig R

更新日期：1973-11-01 00:00:00

abstract：:Receptors for platelet-derived growth factor (PDGF) have previously only been found on cells of mesenchymal and glial origin. This study shows PDGF receptors on an anaplastic thyroid carcinoma cell line, C 643, that was found to express thyroglobulin mRNA, confirming its origin from thyroid epithelium. Northern blot a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heldin NE,Gustavsson B,Claesson-Welsh L,Hammacher A,Mark J,Heldin CH,Westermark B

更新日期：1988-12-01 00:00:00

abstract：:Fluorescent bisretinoids, such as A2E and all-trans-retinal dimer, form as a by-product of vitamin A cycling in retina and accumulate in retinal pigment epithelial (RPE) cells as lipofuscin pigments. These pigments are implicated in pathological mechanisms involved in several vision-threatening diseases including age-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Y,Yanase E,Feng X,Siegel MM,Sparrow JR

更新日期：2010-04-20 00:00:00

abstract：:The c-Jun NH(2)-terminal kinase (JNK) is activated when cells are exposed to environmental stress, including UV radiation. Gene disruption studies demonstrate that JNK is essential for UV-stimulated apoptosis mediated by the mitochondrial pathway by a Bax/Bak-dependent mechanism. Here, we demonstrate that JNK phosphor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lei K,Davis RJ

更新日期：2003-03-04 00:00:00

abstract：:Palmitoylation is a common lipid modification known to regulate the functional properties of various proteins and is a vital step in the biosynthesis of voltage-activated sodium (Nav) channels. We discovered a mutation in an intracellular loop of rNav1.2a (G1079C), which results in a higher apparent affinity for exter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bosmans F,Milescu M,Swartz KJ

更新日期：2011-12-13 00:00:00

abstract：:The members of the cytoplasmic 70-kDa heat shock protein family are involved in appropriate folding and trafficking of newly synthesized proteins in the cell. Hsc70, which is expressed constitutively, and Hsp70, the expression of which is stress- and heat shock-induced, are often considered to have similar cellular fu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldfarb SB,Kashlan OB,Watkins JN,Suaud L,Yan W,Kleyman TR,Rubenstein RC

更新日期：2006-04-11 00:00:00

abstract：:TREK channels produce background currents that regulate cell excitability. These channels are sensitive to a wide variety of stimuli including polyunsaturated fatty acids (PUFAs), phospholipids, mechanical stretch, and intracellular acidification. They are inhibited by neurotransmitters, hormones, and pharmacological ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sandoz G,Bell SC,Isacoff EY

更新日期：2011-02-08 00:00:00

abstract：:We study a simple antiplane fault of finite length embedded in a homogeneous isotropic elastic solid to understand the origin of seismic source heterogeneity in the presence of nonlinear rate- and state-dependent friction. All the mechanical properties of the medium and friction are assumed homogeneous. Friction inclu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Madariaga R,Cochard A

更新日期：1996-04-30 00:00:00

abstract：:McKay et al. [(1996) Science 273, 924-930] suggested that carbonate globules in the meteorite ALH84001 contained the fossil remains of Martian microbes. We have characterized a subpopulation of magnetite (Fe(3)O(4)) crystals present in abundance within the Fe-rich rims of these carbonate globules. We find these Martia...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Keprta KL,Clemett SJ,Bazylinski DA,Kirschvink JL,McKay DS,Wentworth SJ,Vali H,Gibson EK Jr,McKay MF,Romanek CS

更新日期：2001-02-27 00:00:00

abstract：:Natural killer T (NKT) cells provide an innate-type immune response upon T cell receptor interaction with CD1d-presented antigens. We demonstrate through equilibrium tetramer binding and antigen presentation assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galacturonosyl cerami...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu D,Zajonc DM,Fujio M,Sullivan BA,Kinjo Y,Kronenberg M,Wilson IA,Wong CH

更新日期：2006-03-14 00:00:00

abstract：:Extracellular fatty acid incorporation into the phospholipids of Staphylococcus aureus occurs via fatty acid phosphorylation. We show that fatty acid kinase (Fak) is composed of two dissociable protein subunits encoded by separate genes. FakA provides the ATP binding domain and interacts with two distinct FakB protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parsons JB,Broussard TC,Bose JL,Rosch JW,Jackson P,Subramanian C,Rock CO

更新日期：2014-07-22 00:00:00

abstract：:O-GlcNAc is an intracellular posttranslational modification that governs myriad cell biological processes and is dysregulated in human diseases. Despite this broad pathophysiological significance, the biochemical effects of most O-GlcNAcylation events remain uncharacterized. One prevalent hypothesis is that O-GlcNAc m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toleman CA,Schumacher MA,Yu SH,Zeng W,Cox NJ,Smith TJ,Soderblom EJ,Wands AM,Kohler JJ,Boyce M

更新日期：2018-06-05 00:00:00

abstract：:The alphavbeta3 integrins are linked to human bleeding disorders, and pathogenic hantaviruses regulate the function of alphavbeta3 integrins and cause acute vascular diseases. alphavbeta3 integrins are present in either extended (active) or dramatically bent (inactive) structures, and interconversion of alphavbeta3 co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Raymond T,Gorbunova E,Gavrilovskaya IN,Mackow ER

更新日期：2005-01-25 00:00:00