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Activation of integrin beta-subunit I-like domains by one-turn C-terminal alpha-helix deletions.

Abstract：

:Integrins contain two structurally homologous but distantly related domains: an I-like domain that is present in all beta-subunits and an I domain that is present in some alpha-subunits. Atomic resolution and mutagenesis studies of alpha I domains demonstrate a C-terminal, axial displacement of the alpha7-helix that allosterically regulates the shape and affinity of the ligand-binding site. Atomic resolution studies of beta I-like domains have thus far demonstrated no similar alpha7-helix displacement; however, other studies are consistent with the idea that alpha I and beta I-like domains undergo structurally analogous rearrangements. To test the hypothesis that C-terminal, axial displacement of the alpha7-helix, coupled with beta6-alpha7 loop reshaping, activates beta I-like domains, we have mimicked the effect of alpha7-helix displacement on the beta6-alpha7 loop by shortening the alpha7-helix by two independent, four-residue deletions of about one turn of alpha-helix. In the case of integrin alphaLbeta2, each mutant exhibits constitutively high affinity for the physiological ligand intercellular adhesion molecule 1 and full exposure of a beta I-like domain activation-dependent antibody epitope. In the case of analogous mutants in integrin alpha4beta7, each mutant shows the activated phenotype of firm adhesion, rather than rolling adhesion, in shear flow. The results show that integrins that contain or lack alpha I domains share a common pathway of beta I-like domain activation, and they suggest that beta I-like and alpha I domain activation involves structurally analogous alpha7-helix axial displacements.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Yang W,Shimaoka M,Chen J,Springer TA

doi

10.1073/pnas.0307291101

keywords:

subject

Has Abstract

pub_date

2004-02-24 00:00:00

pages

2333-8

issue

8

eissn

0027-8424

issn

1091-6490

pii

101/8/2333

journal_volume

101

pub_type

杂志文章

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