IkappaB-kinasebeta-dependent NF-kappaB activation provides radioprotection to the intestinal epithelium.

abstract：:Understanding the molecular determinants of specificity in protein-protein interaction is an outstanding challenge of postgenome biology. The availability of large protein databases generated from sequences of hundreds of bacterial genomes enables various statistical approaches to this problem. In this context covaria...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weigt M,White RA,Szurmant H,Hoch JA,Hwa T

更新日期：2009-01-06 00:00:00

abstract：:Notwithstanding important advances in the context of single-variant pathogenicity identification, novel breakthroughs in discerning the origins of many rare diseases require methods able to identify more complex genetic models. We present here the Variant Combinations Pathogenicity Predictor (VarCoPP), a machine-learn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papadimitriou S,Gazzo A,Versbraegen N,Nachtegael C,Aerts J,Moreau Y,Van Dooren S,Nowé A,Smits G,Lenaerts T

更新日期：2019-06-11 00:00:00

abstract：:To investigate the contribution of interleukin-4 (IL-4) to airway inflammation in vivo and to explore directly its relationship to airway reactivity, we created transgenic mice in which the murine cDNA for IL-4 was regulated by the rat Clara cell 10 protein promoter. Expression was detected only in the lung and not in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rankin JA,Picarella DE,Geba GP,Temann UA,Prasad B,DiCosmo B,Tarallo A,Stripp B,Whitsett J,Flavell RA

更新日期：1996-07-23 00:00:00

abstract：:Copper is an essential metal nutrient that is tightly regulated in the body because loss of its homeostasis is connected to severe diseases such as Menkes and Wilson diseases, Alzheimer's disease, prion disorders, and amyotrophic lateral sclerosis. The complex relationships between copper status and various stages of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hirayama T,Van de Bittner GC,Gray LW,Lutsenko S,Chang CJ

更新日期：2012-02-14 00:00:00

abstract：:Thrombin induces platelet aggregation and formation of a fibrin clot in platelet-rich plasma; leupeptin, a protease inhibitor, partially inhibits platelet aggregation, but it does not inhibit fibrin clot formation. Indomethacin does not inhibit either thrombin-induced platelet aggregation or fibrin clot formation. How...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruggiero M,Lapetina EG

更新日期：1986-05-01 00:00:00

abstract：:Occurrence of the transversion mutation A.T to C.G is specifically enhanced in Escherichia coli mutT mutants. With the aid of the cloned mutT gene, the MutT protein, which has a molecular mass of 15 kilodaltons, was overproduced and purified to near homogeneity. The protein catalyzes hydrolysis of dGTP to dGMP. dGDP a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama M,Maki H,Sekiguchi M,Horiuchi T

更新日期：1989-06-01 00:00:00

abstract：:Argonaute (Ago) proteins are the key component of the RNA-induced silencing complex and mediate RNA interference (RNAi) in association with small RNAs. Although overall the mechanism of RNAi is well understood, many molecular details of this complex process are not. Here we report about in-depth steady-state and, in p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deerberg A,Willkomm S,Restle T

更新日期：2013-10-29 00:00:00

abstract：:The concentration of urea in renal medullary cells is high enough to affect enzymes seriously by reducing Vmax or raising Km, yet the cells survive and function. The usual explanation is that the methylamines found in the renal medulla, namely glycerophosphocholine and betaine, have actions opposite to those of urea a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burg MB,Peters EM,Bohren KM,Gabbay KH

更新日期：1999-05-25 00:00:00

abstract：:Several cDNA clones encoding the human immunoglobulin G receptor CD16 were isolated from human lung or peripheral blood leukocyte cDNA libraries. Nucleotide sequence comparisons revealed that the cDNAs could be divided into two groups. cDNA clones in one group encode a protein that terminates 4 amino acids after the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scallon BJ,Scigliano E,Freedman VH,Miedel MC,Pan YC,Unkeless JC,Kochan JP

更新日期：1989-07-01 00:00:00

abstract：:Three-prime repair exonuclease 1 knockout (Trex1-/-) mice suffer from systemic inflammation caused largely by chronic activation of the cyclic GMP-AMP synthase-stimulator of interferon genes-TANK-binding kinase-interferon regulatory factor 3 (cGAS-STING-TBK1-IRF3) signaling pathway. We showed previously that Trex1-def...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hasan M,Gonugunta VK,Dobbs N,Ali A,Palchik G,Calvaruso MA,DeBerardinis RJ,Yan N

更新日期：2017-01-24 00:00:00

abstract：:Chronic administration of the atypical antipsychotic drug, clozapine, to rodents has been shown to increase the concentration of apolipoprotein D (apoD) in several area of the brain, suggesting that apoD could be involved in the therapeutic effects of antipsychotic drugs and/or the pathology of psychotic illnesses. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas EA,Dean B,Pavey G,Sutcliffe JG

更新日期：2001-03-27 00:00:00

abstract：:The activation of additional promoter sites by production of an alternative sigma subunit for RNA polymerase is a common strategy for the coordinate regulation of gene expression. Many alternative sigma factors control genes for specialized, and often narrowly distributed, functions. For example, most of the alternati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YF,Helmann JD

更新日期：1992-06-01 00:00:00

abstract：:This report describes the integration of laser-scanning fluorometric cytometry and nonseparation ligand-binding techniques to provide new assay methods adaptable to miniaturization and high-throughput screening. Receptor-bound, cyanine dye-labeled ligands, [Cy]ligands, were discriminated from those free in solution by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zuck P,Lao Z,Skwish S,Glickman JF,Yang K,Burbaum J,Inglese J

更新日期：1999-09-28 00:00:00

abstract：:Tuberous sclerosis complex (TSC) is a tumor suppressor syndrome characterized by benign tumors in multiple organs, including the brain and kidney. TSC-associated tumors exhibit hyperactivation of mammalian target of rapamycin complex 1 (mTORC1), a direct inhibitor of autophagy. Autophagy can either promote or inhibit ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parkhitko A,Myachina F,Morrison TA,Hindi KM,Auricchio N,Karbowniczek M,Wu JJ,Finkel T,Kwiatkowski DJ,Yu JJ,Henske EP

更新日期：2011-07-26 00:00:00

abstract：:Cytoskeletal filaments assemble into dense parallel, antiparallel, or disordered networks, providing a complex environment for active cargo transport and positioning by molecular motors. The interplay between the network architecture and intrinsic motor properties clearly affects transport properties but remains poorl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Richard M,Blanch-Mercader C,Ennomani H,Cao W,De La Cruz EM,Joanny JF,Jülicher F,Blanchoin L,Martin P

更新日期：2019-07-23 00:00:00

abstract：:The guanosine hydrazide 1 yields a stable supramolecular hydrogel based on the formation of a guanine quartet (G-quartet) in presence of metal cations. The effect of various parameters (concentration, nature of metal ion, and temperature) on the properties of this gel has been studied. Proton NMR spectroscopy is shown...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sreenivasachary N,Lehn JM

更新日期：2005-04-26 00:00:00

abstract：:The involvement of chemical exchange in 2D IR heterodyne echo spectroscopy is characterized through the hydrogen-bond exchange between CH3OH and the CN of CH3CN. The exchange dynamics on the hydrogen-bond potential surfaces associated with different quantum states of the high-frequency CN stretching mode contributes t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim YS,Hochstrasser RM

更新日期：2005-08-09 00:00:00

abstract：:A recombinant vaccinia virus that directs the synthesis of bacteriophage T7 RNA polymerase provides the basis for the expression of genes that are regulated by T7 promoters in mammalian cells. The T7 transcripts, which account for as much as 30% of the total cytoplasmic RNA at 24 hr after infection, are largely uncapp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elroy-Stein O,Fuerst TR,Moss B

更新日期：1989-08-01 00:00:00

abstract：:Morphine is one of the most prescribed and effective drugs used for the treatment of acute and chronic pain conditions. In addition to its central effects, morphine can also produce peripheral analgesia. However, the mechanisms underlying this peripheral action of morphine have not yet been fully elucidated. Here, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cunha TM,Roman-Campos D,Lotufo CM,Duarte HL,Souza GR,Verri WA Jr,Funez MI,Dias QM,Schivo IR,Domingues AC,Sachs D,Chiavegatto S,Teixeira MM,Hothersall JS,Cruz JS,Cunha FQ,Ferreira SH

更新日期：2010-03-02 00:00:00

abstract：:The spleen from a patient with adult Gaucher's disease was shown to be deficient in a beta-glucosidase (EC 3.2.1.21) isoenzyme that has optimal activity at pH 4.0-4.3, and is stimulated by 0.02% Triton X-100. A mixture of spleen homogenates from a control and from the patient contained beta-glucosidase activity equiva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho MW,O'Brien JS

更新日期：1971-11-01 00:00:00

abstract：:The interaction of human high density lipoproteins (HDL) with isolated human monocytes from control and Tangier patients was studied in tissue culture experiments. It was observed that normal monocytes, similar to mouse peritoneal macrophages, bind HDL to a cell surface receptor, internalize the bound HDL particles, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmitz G,Assmann G,Robenek H,Brennhausen B

更新日期：1985-09-01 00:00:00

abstract：:Infection during the neonatal period commonly induces apnea episodes, and the proinflammatory cytokine IL-1beta may serve as a critical mediator between these events. To determine the mechanism by which IL-1beta depresses respiration, we examined a prostaglandin E(2) (PGE(2))-dependent pathway in newborn mice and huma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hofstetter AO,Saha S,Siljehav V,Jakobsson PJ,Herlenius E

更新日期：2007-06-05 00:00:00

abstract：:In many adult tissues, mesenchymal stem cells (MSCs) are closely associated with perivascular niches and coexpress many markers in common with pericytes. The ability of pericytes to act as MSCs, however, remains controversial. By using genetic lineage tracing, we show that some pericytes differentiate into specialized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feng J,Mantesso A,De Bari C,Nishiyama A,Sharpe PT

更新日期：2011-04-19 00:00:00

abstract：:The nitrogen isotopic composition (15N/14N) of forested ecosystems varies systematically worldwide. In tropical forests, which are elevated in 15N relative to temperate biomes, a decrease in ecosystem 15N/14N with increasing rainfall has been reported. This trend is seen in a set of well characterized Hawaiian rainfor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Houlton BZ,Sigman DM,Hedin LO

更新日期：2006-06-06 00:00:00

abstract：:Delta 5-3-Ketosteroid isomerase (EC 5.3.3.1) promotes an allylic rearrangement involving intramolecular proton transfer via a dienolic intermediate. This enzyme enhances the catalytic rate by a factor of 10(10). Two residues, Tyr-14, the general acid that polarizes the steroid 3-carbonyl group and facilitates enolizat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao Q,Abeygunawardana C,Talalay P,Mildvan AS

更新日期：1996-08-06 00:00:00

abstract：:When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myers JC,Dobkin C,Spiegelman S

更新日期：1980-03-01 00:00:00

abstract：:Global biological datasets generated by genomics, transcriptomics, and proteomics provide new approaches to understanding the relationship between the genome and the synapse. Combined transcriptome analysis and multielectrode recordings of neuronal network activity were used in mouse embryonic primary neuronal culture...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Valor LM,Charlesworth P,Humphreys L,Anderson CN,Grant SG

更新日期：2007-03-13 00:00:00

abstract：:Human breast cancers contain an RNA related to that of mouse mammary tumor virus. In 79% of the breast malignancies examined, this type of RNA is a 70S-component encapsulated with RNA-instructed DNA polymerase in a particle possessing the density characteristics of RNA tumor viruses. Further, the DNA synthesized by th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Axel R,Gulati SC,Spiegelman S

更新日期：1972-11-01 00:00:00

abstract：:We have developed an efficient, versatile, and user-friendly viral engineering and expression system that is based on in planta assembly of functional viral vectors from separate pro-vector modules. With this new system, instead of supplying a plant cell with a complete viral vector as a mature viral particle, an RNA ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marillonnet S,Giritch A,Gils M,Kandzia R,Klimyuk V,Gleba Y

更新日期：2004-05-04 00:00:00

abstract：:Although malignant astrocytomas are a leading cause of cancer-related death in children, rational therapeutic strategies are lacking. We previously identified activating mutations of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) (BRAF(T1799A) encoding BRAF(V600E)) in association with homozygous cyclin-dependen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huillard E,Hashizume R,Phillips JJ,Griveau A,Ihrie RA,Aoki Y,Nicolaides T,Perry A,Waldman T,McMahon M,Weiss WA,Petritsch C,James CD,Rowitch DH

更新日期：2012-05-29 00:00:00