Ligand-receptor binding measured by laser-scanning imaging.

abstract：:Colon carcinoma is one of the leading causes of death from cancer and is characterized by a heterogenic pool of cells with distinct differentiation patterns. Recently, it was reported that a population of undifferentiated cells from a primary tumor, so-called cancer stem cells (CSC), can reconstitute the original tumo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vermeulen L,Todaro M,de Sousa Mello F,Sprick MR,Kemper K,Perez Alea M,Richel DJ,Stassi G,Medema JP

更新日期：2008-09-09 00:00:00

abstract：:We utilized the presence of hepatic receptors for galactose-terminal glycoproteins to achieve specific rescue of differentiated hepatocytes from methotrexate toxicity. This was accomplished by administration of a conjugate formed by covalent coupling of the methotrexate antagonist, folinic acid (leucovorin), to galact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu GY,Wu CH,Stockert RJ

更新日期：1983-05-01 00:00:00

abstract：:The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amsel AD,Rathaus M,Kronman N,Cohen HY

更新日期：2008-04-01 00:00:00

abstract：:Patients with hematological malignancies can be successfully treated with HLA-matched T cell-depleted allogeneic stem cell transplantation (alloSCT) and subsequent donor lymphocyte infusions (DLIs). The efficacy of DLI is mediated by donor T cells recognizing minor histocompatibility antigens (mHags) on malignant reci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffioen M,van der Meijden ED,Slager EH,Honders MW,Rutten CE,van Luxemburg-Heijs SA,von dem Borne PA,van Rood JJ,Willemze R,Falkenburg JH

更新日期：2008-03-11 00:00:00

abstract：:Creatine kinase (CK; EC 2.7.3.2) plays an important role in energy metabolism in brain and muscle. Expression of CK isoenzymes is regulated during development and is tissue specific. To define the structures of canine CK isoenzymes and to elucidate the mechanism of regulation in their expression, CK cDNA clones from d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roman D,Billadello J,Gordon J,Grace A,Sobel B,Strauss A

更新日期：1985-12-01 00:00:00

abstract：:The quorum sensing signal autoinducer-2 (AI-2) regulates important bacterial behaviors, including biofilm formation and the production of virulence factors. Some bacteria, such as Escherichia coli, can quench the AI-2 signal produced by a variety of species present in the environment, and thus can influence AI-2-depen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marques JC,Oh IK,Ly DC,Lamosa P,Ventura MR,Miller ST,Xavier KB

更新日期：2014-09-30 00:00:00

abstract：:Multiplication-stimulating activity (MSA), purified from medium conditioned by the BRL-3A rat liver cell line, previously has been shown to be closely related to the human somatomedins or insulin-like growth factors. A radioimmunoassay was utilized to measure MSA levels in sera from fetal, maternal, and young rats. A ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moses AC,Nissley SP,Short PA,Rechler MM,White RM,Knight AB,Higa OZ

更新日期：1980-06-01 00:00:00

abstract：:Programmed cell death (apoptosis) is a normal physiological process, which could in principle be manipulated to play an important role in cancer therapy. The key importance of p53 expression in the apoptotic response to DNA-damaging agents has been stressed because mutant or deleted p53 is so common in most kinds of c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu C,Meikrantz W,Schlegel R,Sager R

更新日期：1995-08-15 00:00:00

abstract：:The protein complex formed by the Ca2+ sensor neuronal calcium sensor 1 (NCS-1) and the guanine exchange factor protein Ric8a coregulates synapse number and probability of neurotransmitter release, emerging as a potential therapeutic target for diseases affecting synapses, such as fragile X syndrome (FXS), the most co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mansilla A,Chaves-Sanjuan A,Campillo NE,Semelidou O,Martínez-González L,Infantes L,González-Rubio JM,Gil C,Conde S,Skoulakis EM,Ferrús A,Martínez A,Sánchez-Barrena MJ

更新日期：2017-02-07 00:00:00

abstract：:Examination of the structural basis for antiviral activity, oral pharmacokinetics, and hepatic metabolism among a series of symmetry-based inhibitors of the human immunodeficiency virus (HIV) protease led to the discovery of ABT-538, a promising experimental drug for the therapeutic intervention in acquired immunodefi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempf DJ,Marsh KC,Denissen JF,McDonald E,Vasavanonda S,Flentge CA,Green BE,Fino L,Park CH,Kong XP

更新日期：1995-03-28 00:00:00

abstract：:In monocots, many genes demonstrate a significant negative GC gradient, meaning that the GC content declines along the orientation of transcription. Such a gradient is not observed in the genes of the dicot plant Arabidopsis. In addition, a lack of homology is often observed when comparing the 5' end of the coding reg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang N,Ferguson AA,Slotkin RK,Lisch D

更新日期：2011-01-25 00:00:00

abstract：:Induced pluripotent stem cells (iPS cells) represent a unique tool for the study of the pathophysiology of human disease, because these cells can be differentiated into multiple cell types in vitro and used to generate patient- and tissue-specific disease models. Given the critical role for skeletal muscle insulin res...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iovino S,Burkart AM,Warren L,Patti ME,Kahn CR

更新日期：2016-02-16 00:00:00

abstract：:The antitumor drug Taxol stabilizes microtubules and reduces their dynamicity, promoting mitotic arrest and cell death. Upon assembly of the alpha/beta-tubulin heterodimer, GTP bound to beta-tubulin is hydrolyzed to GDP reaching a steady-state equilibrium between free tubulin dimers and microtubules. The binding of Ta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao H,Verdier-Pinard P,Fernandez-Fuentes N,Burd B,Angeletti R,Fiser A,Horwitz SB,Orr GA

更新日期：2006-07-05 00:00:00

abstract：:The configuration of the heme-carbonyl group upon binding of carbon monoxide to sperm whale myoglobin (Mb) in crystals is evaluated on the basis of infrared spectroscopic methods. Multiplets of the totally symmetric C-O stretching mode are observed for the heme-bound ligand near 1933, 1944, and 1967 cm-1, correspondin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Makinen MW,Houtchens RA,Caughey WS

更新日期：1979-12-01 00:00:00

abstract：:Various tissues and cells in culture contain a specific inhibitor of DNase I (EC 3.1.4.5). In this paper evidence is presented that this inhibitor is actin, one of the major structural proteins of muscle and nonmuscle cells. (a) The inhibitor is a major cellular component constituting 5-10% of the soluble protein. (b)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lazarides E,Lindberg U

更新日期：1974-12-01 00:00:00

abstract：:Fifty cats with feline leukemia virus (FeLV) infection and leukemia-lymphoma complex were treated by ex vivo immunoadsorption with Staphylococcus protein A-bound filters. Most cats responded to therapy. Twelve showed tumor regression, including disappearance of tumor cells, but died later of other complications. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu WT,Engelman RW,Trang LQ,Hau K,Good RA,Day NK

更新日期：1984-06-01 00:00:00

abstract：:The free energy difference between complexes of the restriction nuclease EcoRI with nonspecific DNA and with the enzyme's recognition sequence is linearly dependent on the water chemical potential of the solution, set using several very different solutes, ranging from glycine and glycerol to triethylene glycol and suc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sidorova NY,Rau DC

更新日期：1996-10-29 00:00:00

abstract：:The broad negative consequences of habitat degradation on biodiversity have been studied, but the complex effects of natural-agricultural landscape matrices remain poorly understood. Here we used stable carbon and nitrogen isotopes to detect changes in mammal resource and habitat use and trophic structure between pres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Magioli M,Moreira MZ,Fonseca RCB,Ribeiro MC,Rodrigues MG,Ferraz KMPMB

更新日期：2019-09-10 00:00:00

abstract：:Despite the importance of mitotic cell rounding in tissue development and cell proliferation, there remains a paucity of approaches to investigate the mechanical robustness of cell rounding. Here we introduce ion beam-sculpted microcantilevers that enable precise force-feedback-controlled confinement of single cells w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cattin CJ,Düggelin M,Martinez-Martin D,Gerber C,Müller DJ,Stewart MP

更新日期：2015-09-08 00:00:00

abstract：:Photochemical release (uncaging) of bioactive messengers with three-dimensional spatial resolution in light-scattering media would be greatly facilitated if the photolysis could be powered by pairs of IR photons rather than the customary single UV photons. The quadratic dependence on light intensity would confine the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furuta T,Wang SS,Dantzker JL,Dore TM,Bybee WJ,Callaway EM,Denk W,Tsien RY

更新日期：1999-02-16 00:00:00

abstract：:Starting with assignments of proton nuclear magnetic resonance previously made for oxytocin in deuterated dimethylsulfoxide at 220 MHz, we have assigned resonances for the mammalian antidiuretic hormone, lysine vasopressin. The results demonstrate that spectral assignments of neurohypophyseal hormones and their congen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walter R,Glickson JD,Schwartz IL,Havran RT,Meienhofer J,Urry DW

更新日期：1972-07-01 00:00:00

abstract：:The infection of human cells by laboratory strains of human immunodeficiency virus type 1 (HIV-1) can be blocked readily in vitro by recombinant soluble CD4 and CD4-immunoglobulin hybrid molecules. In contrast, infection by primary isolates of HIV-1 is much less sensitive to blocking in vitro by soluble CD4-based mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ashkenazi A,Smith DH,Marsters SA,Riddle L,Gregory TJ,Ho DD,Capon DJ

更新日期：1991-08-15 00:00:00

abstract：:The molecular mechanism by which the homodimeric enzyme Cu/Zn superoxide dismutase (SOD) causes neural damage in amytrophic lateral sclerosis is yet poorly understood. A striking, as well as an unusual, feature of SOD is that it maintains intrasubunit disulfide bonds in the reducing environment of the cytosol. Here, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lindberg MJ,Normark J,Holmgren A,Oliveberg M

更新日期：2004-11-09 00:00:00

abstract：:Tomato plants overexpressing a prosystemin gene that encodes the precursor of a mobile wound signal called systemin have been shown previously to constitutively synthesize extraordinarily high levels of two defensive proteinase inhibitor proteins in leaves in the absence of wounding. We herein report that leaves of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Constabel CP,Bergey DR,Ryan CA

更新日期：1995-01-17 00:00:00

abstract：:Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and solutes are internalized into cells. Macropinocytosis starts with the formation of membrane ruffles at the plasma membrane and ends with their closure. The transient and sequential emergence of phosphoinositides PI(3,4,5)P3 and P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maekawa M,Terasaka S,Mochizuki Y,Kawai K,Ikeda Y,Araki N,Skolnik EY,Taguchi T,Arai H

更新日期：2014-03-18 00:00:00

abstract：:With Manduca sexta as a model system, we analyzed how natural odor mixtures that are most effective in eliciting flight and foraging behaviors are encoded in the primary olfactory center in the brain, the antennal lobe. We used gas chromatography coupled with multiunit neural-ensemble recording to identify key odorant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Riffell JA,Lei H,Hildebrand JG

更新日期：2009-11-17 00:00:00

abstract：:Let G be a reductive algebraic group and let B be an affine variety with an algebraic action of G. Everything is defined over the field C of complex numbers. Consider the trivial G-vector bundle B x S = S over B where S is a G-module. From the endomorphism ring R of the G-vector bundle S a construction of G-vector bun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masuda M,Petrie T

更新日期：1991-10-15 00:00:00

abstract：:Marine viruses are the most abundant biological entities in the oceans shaping community structure and nutrient cycling. The interaction between the bloom-forming alga Emiliania huxleyi and its specific large dsDNA virus (EhV) is a major factor determining the fate of carbon in the ocean, thus serving as a key host-pa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ziv C,Malitsky S,Othman A,Ben-Dor S,Wei Y,Zheng S,Aharoni A,Hornemann T,Vardi A

更新日期：2016-03-29 00:00:00

abstract：:We have examined whether dissociation of the histone octamer is required for elongation of RNA transcripts through arrays of nucleosome cores in vitro. Control or dimethyl suberimidate-crosslinked histone octamers were reconstituted onto supercoiled, closed circular pT207-18 DNA, which contains tandemly repeated 207-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Neill TE,Smith JG,Bradbury EM

更新日期：1993-07-01 00:00:00

abstract：:Although the transport sector is responsible for a large and growing share of global emissions affecting climate, its overall contribution has not been quantified. We provide a comprehensive analysis of radiative forcing from the road transport, shipping, aviation, and rail subsectors, using both past- and forward-loo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuglestvedt J,Berntsen T,Myhre G,Rypdal K,Skeie RB

更新日期：2008-01-15 00:00:00