Abstract:
:Marine viruses are the most abundant biological entities in the oceans shaping community structure and nutrient cycling. The interaction between the bloom-forming alga Emiliania huxleyi and its specific large dsDNA virus (EhV) is a major factor determining the fate of carbon in the ocean, thus serving as a key host-pathogen model system. The EhV genome encodes for a set of genes involved in the de novo sphingolipid biosynthesis, not reported in any viral genome to date. We combined detailed lipidomic and biochemical analyses to characterize the functional role of this virus-encoded pathway during lytic viral infection. We identified a major metabolic shift, mediated by differential substrate specificity of virus-encoded serine palmitoyltransferase, a key enzyme of sphingolipid biosynthesis. Consequently, unique viral glycosphingolipids, composed of unusual hydroxylated C17 sphingoid bases (t17:0) were highly enriched in the infected cells, and their synthesis was found to be essential for viral assembly. These findings uncover the biochemical bases of the virus-induced metabolic rewiring of the host sphingolipid biosynthesis during the chemical "arms race" in the ocean.
journal_name
Proc Natl Acad Sci U S Aauthors
Ziv C,Malitsky S,Othman A,Ben-Dor S,Wei Y,Zheng S,Aharoni A,Hornemann T,Vardi Adoi
10.1073/pnas.1523168113subject
Has Abstractpub_date
2016-03-29 00:00:00pages
E1907-16issue
13eissn
0027-8424issn
1091-6490pii
1523168113journal_volume
113pub_type
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