Ablation of P/Q-type Ca(2+) channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the alpha(1A)-subunit.

abstract：:The regular spacing of somites during vertebrate embryogenesis involves a dynamic gradient of FGF signaling that controls the timing of maturation of cells in the presomitic mesoderm (PSM). How the FGF signal is transduced by PSM cells is unclear. Here, we first show that the FGF gradient is translated into graded act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Delfini MC,Dubrulle J,Malapert P,Chal J,Pourquié O

更新日期：2005-08-09 00:00:00

abstract：:When infants and adults communicate, they exchange social signals of availability and communicative intention such as eye gaze. Previous research indicates that when communication is successful, close temporal dependencies arise between adult speakers' and listeners' neural activity. However, it is not known whether s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leong V,Byrne E,Clackson K,Georgieva S,Lam S,Wass S

更新日期：2017-12-12 00:00:00

abstract：:High-affinity ligands of the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1) were isolated by the SELEX procedure (systematic evolution of ligands by exponential enrichment) from RNA populations randomized at 32 positions. Analysis of these ligands revealed a pseudoknot consensus with primary sequ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tuerk C,MacDougal S,Gold L

更新日期：1992-08-01 00:00:00

abstract：:We are interested in using recombinant adeno-associated viral vectors in the treatment of hemophilia A. Because of the size constraints of recombinant adeno-associated viral vectors, we delivered the heavy and light chains of the human factor 8 (hFVIII) cDNA independently by using two separate vectors. Recombinant AAV...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burton M,Nakai H,Colosi P,Cunningham J,Mitchell R,Couto L

更新日期：1999-10-26 00:00:00

abstract：:The neuronal isoform of nitric oxide synthase (nNOS) is highly expressed in mammalian skeletal muscle, but its functional role has not been defined. NO has been implicated in the local metabolic regulation of blood flow in contracting skeletal muscle in part by antagonizing sympathetic vasoconstriction. We therefore h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas GD,Sander M,Lau KS,Huang PL,Stull JT,Victor RG

更新日期：1998-12-08 00:00:00

abstract：:The pathogenicity of fixed rabies virus strains for adult mice depends on the presence of an antigenic determinant on the viral glycoprotein. Two virus-neutralizing monoclonal antibodies have been used to identify this determinant. All pathogenic strains of fixed rabies virus bind to these antibodies and are neutraliz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dietzschold B,Wunner WH,Wiktor TJ,Lopes AD,Lafon M,Smith CL,Koprowski H

更新日期：1983-01-01 00:00:00

abstract：:Allozyme, morphological, and PCR-generated mtDNA markers were used to analyze hybrid parentage and introgression between the neighboringly sympatric crustaceans Daphnia galeata mendotae and Daphnia rosea. mtDNA analysis of D. galeata mendotae from 15 lakes and of D. rosea from 8 lakes revealed species-specific fragmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taylor DJ,Hebert PD

更新日期：1993-08-01 00:00:00

abstract：:Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that has a progression that is closely associated with oxidative stress. It has long been speculated that the reactive oxygen species (ROS) level in AD brains is much higher than that in healthy brains. However, evidence from living beings is scarc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang J,Zhang X,Yuan P,Yang J,Xu Y,Grutzendler J,Shao Y,Moore A,Ran C

更新日期：2017-11-21 00:00:00

abstract：:The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of repli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami Y,Wobbe CR,Weissbach L,Dean FB,Hurwitz J

更新日期：1986-05-01 00:00:00

abstract：:Fluoxetine (Prozac) inhibited the membrane currents elicited by serotonin (5-hydroxytryptamine; 5HT) in Xenopus oocytes expressing either cloned 5HT2C receptors or 5HT receptors encoded by rat cortex mRNA. Responses of 5HT2C receptors, elicited by nM concentrations of 5HT, were rapidly and reversibly blocked by microm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ni YG,Miledi R

更新日期：1997-03-04 00:00:00

abstract：:The selective alteration of the cellular genome by laser microbeam irradiation has been extensively applied in cell biology. We report here the use of the third harmonic (355 nm) of an yttrium-aluminum garnet laser to facilitate the direct transfer of the neo gene into cultured human HT1080-6TG cells. The resultant tr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tao W,Wilkinson J,Stanbridge EJ,Berns MW

更新日期：1987-06-01 00:00:00

abstract：:One of the critical issues regarding proteins with a four-helix bundle motif is which interactions play the major role in stabilizing this type of folded structure: the interaction among the four alpha-helices or the interaction between the loop and helix segments. To answer this question, an energetic analysis has be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chou KC,Maggiora GM,Scheraga HA

更新日期：1992-08-15 00:00:00

abstract：:Sparse sensor placement is a central challenge in the efficient characterization of complex systems when the cost of acquiring and processing data is high. Leading sparse sensing methods typically exploit either spatial or temporal correlations, but rarely both. This work introduces a sparse sensor optimization that i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mohren TL,Daniel TL,Brunton SL,Brunton BW

更新日期：2018-10-16 00:00:00

abstract：:Protein misfolding and deposition underlie an increasing number of debilitating human disorders. We have shown that model proteins unrelated to disease, such as the Src homology 3 (SH3) domain of the p58alpha subunit of bovine phosphatidyl-inositol-3'-kinase (PI3-SH3), can be converted in vitro into assemblies with st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ventura S,Zurdo J,Narayanan S,Parreño M,Mangues R,Reif B,Chiti F,Giannoni E,Dobson CM,Aviles FX,Serrano L

更新日期：2004-05-11 00:00:00

abstract：:Using an antibody highly specific for D-serine conjugated to glutaraldehyde, we have localized endogenous D-serine in rat brain. Highest levels of D-serine immunoreactivity occur in the gray matter of the cerebral cortex, hippocampus, anterior olfactory nucleus, olfactory tubercle, and amygdala. Localizations of D-ser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schell MJ,Molliver ME,Snyder SH

更新日期：1995-04-25 00:00:00

abstract：:The first step in the formation of the protease resistant form (PrPSc) of prion proteins involves a conformational transition of the monomeric cellular form of PrPC to a more stable aggregation prone state PrPC*. A search of PDBselect and Escherichia coli and yeast genomes shows that the exact pattern of charges in he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dima RI,Thirumalai D

更新日期：2004-10-26 00:00:00

abstract：:The photoaffinity labeling agent 5-azidoindole-3-acetic acid, an analog of the endogenous plant hormone indole-3-acetic acid (an auxin), was used to identify indole-3-acetic acid-binding proteins in maize. Two peptides with subunit molecular masses of 24 and 22 kilodaltons are specifically labeled in a saturable manne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones AM,Venis MA

更新日期：1989-08-01 00:00:00

abstract：:A variety of peptide-secreting endocrine cells contain a population of recycling microvesicles that share several major membrane polypeptides with neuronal synaptic vesicles (SVs). The function of these synaptic-like microvesicles (SLMVs) remains to be elucidated. It was previously suggested that SLMVs of pancreatic b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas-Reetz A,Hell JW,During MJ,Walch-Solimena C,Jahn R,De Camilli P

更新日期：1993-06-01 00:00:00

abstract：:NY-ESO-1 is a "cancer-testis" antigen frequently expressed in epithelial ovarian cancer (EOC) and is among the most immunogenic tumor antigens defined to date. In an effort to understand in vivo tolerance mechanisms, we assessed the phenotype and function of NY-ESO-1-specific CD8(+) T cells derived from peripheral blo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuzaki J,Gnjatic S,Mhawech-Fauceglia P,Beck A,Miller A,Tsuji T,Eppolito C,Qian F,Lele S,Shrikant P,Old LJ,Odunsi K

更新日期：2010-04-27 00:00:00

abstract：:Some spectinomycin-resistant mutants of Escherichia coli are cold-sensitive. They are unable to assemble both 30S and 50S ribosomal subunits at low temperatures. They accumulate two kinds of incomplete particles, related to 30S and 50S subunits respectively. A single mutation, causing an alteration in a 30S ribosomal ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nashimoto H,Nomura M

更新日期：1970-11-01 00:00:00

abstract：:A discussion of the expected mechanism of radical reduction of carbonyl compounds by dihydroflavin as it relates to the structure of the carbonyl compound is provided. Factors which must be taken into account are the free energies of formation of the radical anions (CR2O), THE ACIDITY OF THE CONJUGATE ACID OF THE CARB...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams RF,Shinkai S,Bruice TC

更新日期：1975-05-01 00:00:00

abstract：:gamma-Aminobutyric acid type A (GABA-A) receptors are a major mediator of inhibitory neurotransmission in the mammalian central nervous system, and the site of action of a number of clinically important drugs. These receptors exist as a family of subtypes with distinct temporal and spatial patterns of expression and d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonnert TP,McKernan RM,Farrar S,le Bourdellès B,Heavens RP,Smith DW,Hewson L,Rigby MR,Sirinathsinghji DJ,Brown N,Wafford KA,Whiting PJ

更新日期：1999-08-17 00:00:00

abstract：:G protein-coupled receptors (GPCRs) have been found as monomers but also as dimers or higher-order oligomers in cells. The relevance of the monomeric or dimeric receptor state for G protein activation is currently under debate for class A rhodopsin-like GPCRs. Clarification of this issue requires the availability of w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White JF,Grodnitzky J,Louis JM,Trinh LB,Shiloach J,Gutierrez J,Northup JK,Grisshammer R

更新日期：2007-07-17 00:00:00

abstract：:The light-harvesting chlorophyll a/b protein (LHCP) is an integral thylakoid membrane protein. It is made in the cytosol as a precursor (pLHCP), imported into chloroplasts, and subsequently integrated into thylakoids. Integration of pLHCP into thylakoids requires a stromal protein factor that functions in part to main...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yuan J,Henry R,Cline K

更新日期：1993-09-15 00:00:00

abstract：:Regulation of the turnover of triglycerides in adipose tissue requires the continuous provision of 3-glycerophosphate, which may be supplied by the metabolism of glucose or by glyceroneogenesis, the de novo synthesis of 3-glycerophosphate from sources other than hexoses or glycerol. The importance of glyceroneogenesis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olswang Y,Cohen H,Papo O,Cassuto H,Croniger CM,Hakimi P,Tilghman SM,Hanson RW,Reshef L

更新日期：2002-01-22 00:00:00

abstract：:Converging TGF-beta and insulin-like neuroendocrine signaling pathways regulate whether Caenorhabditis elegans develops reproductively or arrests at the dauer larval stage. We examined whether neurotransmitters act in the dauer entry or recovery pathways. Muscarinic agonists promote recovery from dauer arrest induced ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tissenbaum HA,Hawdon J,Perregaux M,Hotez P,Guarente L,Ruvkun G

更新日期：2000-01-04 00:00:00

abstract：:The α-proteobacterium Sinorhizobium meliloti establishes a chronic intracellular infection during the symbiosis with its legume hosts. Within specialized host cells, S. meliloti differentiates into highly polyploid, enlarged nitrogen-fixing bacteroids. This differentiation is driven by host cells through the productio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Penterman J,Abo RP,De Nisco NJ,Arnold MF,Longhi R,Zanda M,Walker GC

更新日期：2014-03-04 00:00:00

abstract：:This study was undertaken to characterize bile acid metabolism in hereditary forms of hypertriglyceridemia. Ten hypertriglyceridemic patients (type IV phenotype) with familial combined hyperlipidemia and 7 patients with monogenic familial hypertriglyceridemia (FHTG) were compared with 18 healthy controls; all subjects...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angelin B,Hershon KS,Brunzell JD

更新日期：1987-08-01 00:00:00

abstract：:Cells are exposed to changes in extracellular stimulus concentration that vary as a function of rate. However, how cells integrate information conveyed from stimulation rate along with concentration remains poorly understood. Here, we examined how varying the rate of stress application alters budding yeast mitogen-act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson AN,Li G,Jashnsaz H,Thiemicke A,Kesler BK,Rogers DC,Neuert G

更新日期：2021-01-12 00:00:00

abstract：:Synthesis of mouse metallothionein (MT)-I and MT-II is transcriptionally induced by the synthetic glucocorticoid, dexamethasone (DEX) or both in vivo as well as in numerous cell lines. However, the location(s) of a glucocorticoid response element (GRE) has not been described. The observation that a marked MT-I gene, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly EJ,Sandgren EP,Brinster RL,Palmiter RD

更新日期：1997-09-16 00:00:00