Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction.

abstract：:HeLa cells grown in the presence of [methyl-3H]thymidine contained large amounts of 5-hydroxymethyl-2'-deoxyuridine (HMdU) in their DNA. When the cells were grown in [6-3H]thymidine and their DNA was labeled to the same specific activity, no HMdU was present. When such [6-3H]thymidine-labeled cells were exposed to inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Teebor GW,Frenkel K,Goldstein MS

更新日期：1984-01-01 00:00:00

abstract：:We have isolated, cloned, and expressed a male antennae-specific pheromone-degrading enzyme (PDE) [Antheraea polyphemus PDE (ApolPDE), formerly known as Sensillar Esterase] from the wild silkmoth, A. polyphemus, which seems essential for the rapid inactivation of pheromone during flight. The onset of enzymatic activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida Y,Leal WS

更新日期：2005-09-27 00:00:00

abstract：:Proteins potentiated by epidermal growth factor (EGF) to induce a transformed phenotype in non-neoplastic rat kidney fibroblasts in cell culture have been isolated from many non-neoplastic tissues of the adult mouse, including submaxillary gland, kidney, liver, muscle, heart, and brain. They resemble previously descri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts AB,Anzano MA,Lamb LC,Smith JM,Sporn MB

更新日期：1981-09-01 00:00:00

abstract：:N6-Cyclohexyl[3H]adenosine ([3H]CHA) and 1,3-diethyl-8-[3H]phenylxanthine ([3H]DPX) to bind to adenosine receptors in brain membranes. The agonist [3H]CHA has high affinity in both bovine and guinea pig brain (Kd, 0.7 nM and 6 nM, respectively). [3H]CHA binding kinetics are slow (dissociation t1/2;60 min); binding is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruns RF,Daly JW,Snyder SH

更新日期：1980-09-01 00:00:00

abstract：:We have identified and isolated a presumptive leukemogenic provirus from myeloblasts of a chicken in which leukemia had been induced by avian myeloblastosis virus (AMV). Leukemic myeloblasts isolated from peripheral blood or from converted yolk sac cultures of various strains of chickens, regardless of the endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Souza LM,Komaromy MC,Baluda MA

更新日期：1980-05-01 00:00:00

abstract：:Soret-excited resonance Raman spectroscopy yields direct information regarding the iron-carbon bonding interactions in the cyanomet and carbonmonoxy complexes of hemoglobin III from Chironomus thummi thummi (CTT III) in solution. By isotope exchange in cyanide (13CN-, C15N-, and 13C15N-) and carbon monoxide (13CO, C18...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yu NT,Benko B,Kerr EA,Gersonde K

更新日期：1984-08-01 00:00:00

abstract：:DsrA is an 87-nucleotide regulatory RNA of Escherichia coli that acts in trans by RNA-RNA interactions with two different mRNAs, hns and rpoS. DsrA has opposite effects on these transcriptional regulators. H-NS levels decrease, whereas RpoS (final sigma(s)) levels increase. Here we show that DsrA enhances hns mRNA tur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lease RA,Belfort M

更新日期：2000-08-29 00:00:00

abstract：:We provide evidence for an interaction between mouse class I major histocompatibility complex antigens and insulin receptors. Antibodies against class I but not class II major histocompatibility complex antigens immunoprecipitate photoaffinity-labeled hepatic insulin receptors. Haplotype specificity is demonstrated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillips ML,Moule ML,Delovitch TL,Yip CC

更新日期：1986-05-01 00:00:00

abstract：:In response to environmental stresses, the mammalian serine threonine kinases PERK, GCN2, HRI, and PKR phosphorylate the regulatory serine 51 of the eukaryotic translation initiation factor 2α (eIF2α) to inhibit global protein synthesis. Plasmodium, the protozoan that causes malaria, expresses three eIF2α kinases: IK1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang M,Mishra S,Sakthivel R,Rojas M,Ranjan R,Sullivan WJ Jr,Fontoura BM,Ménard R,Dever TE,Nussenzweig V

更新日期：2012-03-06 00:00:00

abstract：:Despite enormous body plan variation, genes regulating embryonic development are highly conserved. Here, we probe the mechanisms that predispose ancient regulatory genes to reutilization and diversification rather than evolutionary loss. The Hox gene fushi tarazu (ftz) arose as a homeotic gene but functions as a pair-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heffer A,Xiang J,Pick L

更新日期：2013-02-05 00:00:00

abstract：:The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin-conjugating enzyme (E2) that is required for DNA repair, damage-induced mutagenesis, and sporulation. We have cloned the two human RAD6 homologs, designated HHR6A and HHR6B. The two 152-amino acid human proteins share 95% sequence identity with each other a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koken MH,Reynolds P,Jaspers-Dekker I,Prakash L,Prakash S,Bootsma D,Hoeijmakers JH

更新日期：1991-10-15 00:00:00

abstract：:The distribution of enkephalin-like immunoreactivity within the avian retina was studied by immunofluorescence and immunoperoxidase techniques with antiserum to [Met5]enkephalin and [Leu5]enkephalin. Formaldehyde-fixed retinae were sectioned and incubated in antiserum to either [Met5]- or [Leu5]enkephalin. Specificity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brecha N,Karten HJ,Laverack C

更新日期：1979-06-01 00:00:00

abstract：:Neuropeptide B (NPB) and neuropeptide W (NPW) have been recently identified as ligands for the G protein-coupled receptor (GPR) 7 and GPR8. The precise in vivo role of this neuropeptide-receptor pathway has not been fully demonstrated. In this paper, we report that NPB-deficient mice manifest a mild adult-onset obesit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly MA,Beuckmann CT,Williams SC,Sinton CM,Motoike T,Richardson JA,Hammer RE,Garry MG,Yanagisawa M

更新日期：2005-07-12 00:00:00

abstract：:Intracellular Ca(2+) release in many types of cells is mediated by ryanodine receptor Ca(2+) release channels (RyRCs) that are assembled into two-dimensional paracrystalline arrays in the endoplasmic/sarcoplasmic reticulum. However, the in situ operating mechanism of the RyRC array is unknown. Here, we found that the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang SQ,Stern MD,Ríos E,Cheng H

更新日期：2004-03-16 00:00:00

abstract：:While 9-O-acetylation of sialic acids has been reported in some mammalian tissues, the distribution of this modification on specific cell types and molecules is largely unknown. The influenza C virus hemagglutinin-esterase is a membrane-bound glycoprotein that binds specifically to 9-O-acetylated sialic acids (hemaggl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein A,Krishna M,Varki NM,Varki A

更新日期：1994-08-02 00:00:00

abstract：:Previous studies imply that the intracellular domain of Notch1 must translocate to the nucleus for its activity. In this study, we demonstrate that a mNotch1 mutant protein that lacks its extracellular domain but retains its membrane-spanning region becomes proteolytically processed on its intracellular surface and, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kopan R,Schroeter EH,Weintraub H,Nye JS

更新日期：1996-02-20 00:00:00

abstract：:The ecological niche is a key concept for elucidating patterns of species distributions and developing strategies for conserving biodiversity. However, recent times are seeing a widespread debate whether species niches are conserved across space and time (niche conservatism hypothesis). Biological invasions represent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu C,Wolter C,Xian W,Jeschke JM

更新日期：2020-09-22 00:00:00

abstract：:Shoot architecture is critical for optimizing plant adaptation and productivity. In contrast with annuals, branching in perennials native to temperate and boreal regions must be coordinated with seasonal growth cycles. How branching is coordinated with seasonal growth is poorly understood. We identified key components...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maurya JP,Miskolczi PC,Mishra S,Singh RK,Bhalerao RP

更新日期：2020-05-26 00:00:00

abstract：:Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masker WE,Hanawalt PC

更新日期：1973-01-01 00:00:00

abstract：:Hormones and nutrients often induce genetic programs via signaling pathways that interface with gene-specific activators. Activation of the cAMP pathway, for example, stimulates cellular gene expression by means of the PKA-mediated phosphorylation of cAMP-response element binding protein (CREB) at Ser-133. Here, we us...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang X,Odom DT,Koo SH,Conkright MD,Canettieri G,Best J,Chen H,Jenner R,Herbolsheimer E,Jacobsen E,Kadam S,Ecker JR,Emerson B,Hogenesch JB,Unterman T,Young RA,Montminy M

更新日期：2005-03-22 00:00:00

abstract：:Major changes in the mRNA population of murine liver occur after administration of bacterial lipopolysaccharide, an agent that causes increases in the concentrations of acute-phase serum proteins. The mRNA for one of these, serum amyloid A, is increased at least 500-fold compared to the normal level. It becomes one of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrow JF,Stearman RS,Peltzman CG,Potter DA

更新日期：1981-08-01 00:00:00

abstract：:After treatment with nitrite, Japanese soy sauce was strongly mutagenic to Salmonella typhimurium TA100 without S9 mixture. Two precursors of the mutagen were isolated from Japanese soy sauce, and these were identified as (-)-(1S,3S)-1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid [(-)-(1S,3S)-MTCA] and i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakabayashi K,Ochiai M,Saitô H,Tsuda M,Suwa Y,Nagao M,Sugimura T

更新日期：1983-05-01 00:00:00

abstract：:Let E and F be vector bundles and D:E --> F an operator of order k. We associate a sequence of invariants ((l))(D), l >/= 0 with D which generalize the concept of curvature in a natural way. In the case where D = D(theta) is the differential operator of a connection theta on a vector bundle E, ((1))(D(theta)) is the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knapp RE

更新日期：1973-03-01 00:00:00

abstract：:The biconcave disk shape and deformability of mammalian RBCs rely on the membrane skeleton, a viscoelastic network of short, membrane-associated actin filaments (F-actin) cross-linked by long, flexible spectrin tetramers. Nonmuscle myosin II (NMII) motors exert force on diverse F-actin networks to control cell shapes,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith AS,Nowak RB,Zhou S,Giannetto M,Gokhin DS,Papoin J,Ghiran IC,Blanc L,Wan J,Fowler VM

更新日期：2018-05-08 00:00:00

abstract：:Mitogen-activated protein (MAP) kinase kinase is an enzyme that activates the growth factor-regulated MAP kinase in vitro by a mechanism that involves direct phosphorylation of MAP kinase on tyrosine and threonine residues. MAP kinase kinase is stimulated by growth factor treatment of cells and has been shown to be in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahn NG,Campbell JS,Seger R,Jensen AL,Graves LM,Krebs EG

更新日期：1993-06-01 00:00:00

abstract：:Although an inverse relationship is expected in ancient DNA samples between the number of surviving DNA fragments and their length, ancient DNA sequencing libraries are strikingly deficient in molecules shorter than 40 bp. We find that a loss of short molecules can occur during DNA extraction and present an improved s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dabney J,Knapp M,Glocke I,Gansauge MT,Weihmann A,Nickel B,Valdiosera C,García N,Pääbo S,Arsuaga JL,Meyer M

更新日期：2013-09-24 00:00:00

abstract：:Subtractive library construction and differential screening were used to identify a cDNA for a cell type-specific immediate early gene induced in rat PC12 pheochromocytoma cells. Sequencing identified the protein product of this gene as rat synaptotagmin IV (SytIV). Synaptotagmins are synaptic vesicle proteins thought...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vician L,Lim IK,Ferguson G,Tocco G,Baudry M,Herschman HR

更新日期：1995-03-14 00:00:00

abstract：:By using a combination of genetic, pharmacological, and anatomical approaches, we show that the melanocortin 4 receptor (MC4R), implicated in the control of food intake and energy expenditure, also modulates erectile function and sexual behavior. Evidence supporting this notion is based on several findings: (i) a high...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van der Ploeg LH,Martin WJ,Howard AD,Nargund RP,Austin CP,Guan X,Drisko J,Cashen D,Sebhat I,Patchett AA,Figueroa DJ,DiLella AG,Connolly BM,Weinberg DH,Tan CP,Palyha OC,Pong SS,MacNeil T,Rosenblum C,Vongs A,Tang R

更新日期：2002-08-20 00:00:00

abstract：:Our long-term goal is the in vivo expression of intrinsically colored proteins without the need for further posttranslational modification or chemical functionalization by externally added reagents. Biocompatible (Aza)Indoles (Inds)/(Aza)Tryptophans (Trp) as optical probes represent almost ideal isosteric substitutes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lepthien S,Hoesl MG,Merkel L,Budisa N

更新日期：2008-10-21 00:00:00

abstract：:Chemical labeling of proteins inside of living cells can enable studies of the location, movement, and function of proteins in vivo. Here we demonstrate an approach for chemical labeling of proteins that uses the high-affinity interaction between an FKBP12 mutant (F36V) and a synthetic, engineered ligand (SLF'). A flu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marks KM,Braun PD,Nolan GP

更新日期：2004-07-06 00:00:00