Ionizing radiation and tritium transmutation both cause formation of 5-hydroxymethyl-2'-deoxyuridine in cellular DNA.

abstract：:Bioluminescence-based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gregor C,Pape JK,Gwosch KC,Gilat T,Sahl SJ,Hell SW

更新日期：2019-12-02 00:00:00

abstract：:A full-length placental alkaline phosphatase (PLAP) cDNA was used to identify and clone the PLAP-like Nagao isozyme gene from human genomic libraries. The entire nucleotide sequence of the gene reveals the existence of 11 exons interrupted by 10 small introns (76-427 base pairs). Putative regulatory sequences have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millán JL,Manes T

更新日期：1988-05-01 00:00:00

abstract：:The functions of individual basal transcription factors during the formation of an initiation complex by RNA polymerase II remain largely unknown. Transcription factor IIE (TFIIE) has recently been shown to bind to multiple targets in the initiation complex. To assess the role of zinc binding in basal transcription, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maxon ME,Tjian R

更新日期：1994-09-27 00:00:00

abstract：:For reasons that are unclear the production of embryonic stem cells from ungulates has proved elusive. Here, we describe induced pluripotent stem cells (iPSC) derived from porcine fetal fibroblasts by lentiviral transduction of 4 human (h) genes, hOCT4, hSOX2, hKLF4, and hc-MYC, the combination commonly used to create...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ezashi T,Telugu BP,Alexenko AP,Sachdev S,Sinha S,Roberts RM

更新日期：2009-07-07 00:00:00

abstract：:The crown gall teratoma tumor line BT37, incited by Agrobacterium tumefaciens strain T37, has been found to contain part of the tumor-inducing plasmid, pTi T37, of the inciting strain. This foreign DNA segment, called T-DNA, is maintained at several copies per diploid tumor cell. We have examined subcellular DNA fract...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chilton MD,Saiki RK,Yadav N,Gordon MP,Quetier F

更新日期：1980-07-01 00:00:00

abstract：:Synergism between transcriptional activators is a powerful way of potentiating their function. Here we show that the glial POU domain protein Tst-1 (also known as Oct-6 and SCIP) and large tumor antigen (T antigen) synergistically increased transcription from both the early and the late promoters of papovavirus JC in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Renner K,Leger H,Wegner M

更新日期：1994-07-05 00:00:00

abstract：:Stromal cell lines were established from canine long-term marrow cultures, cloned by limiting dilution, and maintained in stromal cell-conditioned medium. These cells grew adherent, maintained stable growth rate and morphology under standard conditions (in 20-30% conditioned medium; confluency, 70-90%), and supported ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huss R,Hong DS,McSweeney PA,Hoy CA,Deeg HJ

更新日期：1995-01-31 00:00:00

abstract：:Sunlight exposure is strongly indicated as one of the important etiologic agents in human cutaneous malignant melanoma. However, because of the absence of good animal models, it has not been possible to estimate the wavelengths or wavelength regions involved. We have developed a useful animal model from crosses and ba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Setlow RB,Woodhead AD,Grist E

更新日期：1989-11-01 00:00:00

abstract：:The core enzyme of Escherichia coli RNA polymerase acquires essential promoter recognition and transcription initiation activities by binding one of several sigma subunits. To characterize the proximity between sigma70, the major sigma for transcription of the growth-related genes, and the core enzyme subunits (alpha2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Owens JT,Miyake R,Murakami K,Chmura AJ,Fujita N,Ishihama A,Meares CF

更新日期：1998-05-26 00:00:00

abstract：:T7 gene 5 DNA polymerase (gp5) and its processivity factor, Escherichia coli thioredoxin, together with the T7 gene 4 DNA helicase, catalyze strand displacement synthesis on duplex DNA processively (>17,000 nucleotides per binding event). The processive DNA synthesis is resistant to the addition of a DNA trap. However...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson DE,Takahashi M,Hamdan SM,Lee SJ,Richardson CC

更新日期：2007-03-27 00:00:00

abstract：:neurogenin2 (ngn2) encodes a basic helix-loop-helix transcription factor and plays an important role in neurogenesis from migratory neural crest cells. Its role in retinal development is poorly understood. We observed that in the developing chick retina, ngn2 was expressed in a subpopulation of proliferating progenito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan RT,Ma WX,Wang SZ

更新日期：2001-12-18 00:00:00

abstract：:We have studied hepatic uptake of chylomicron retinyl ester. Chylomicrons were obtained from intestinal lymph of rats that were given retinol in groundnut oil by intraduodenal injection. When lymph was injected intravenously into normal rats, the radioactivity was cleared from blood with t1/2 approximately equal to 10...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blomhoff R,Helgerud P,Rasmussen M,Berg T,Norum KR

更新日期：1982-12-01 00:00:00

abstract：:Huntington's disease (HD) is a progressive neurodegenerative disease caused by a glutamine expansion within huntingtin protein. The exact pathological mechanisms determining disease onset and progression remain unclear. However, aggregates of insoluble mutant huntingtin (mhtt), a hallmark of HD, are readily detected w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chopra V,Fox JH,Lieberman G,Dorsey K,Matson W,Waldmeier P,Housman DE,Kazantsev A,Young AB,Hersch S

更新日期：2007-10-16 00:00:00

abstract：:Chromatin states can be distinguished by differential covalent modifications of histones or by utilization of histone variants. Chromatin associated with transcriptionally active loci becomes enriched for histones with particular lysine modifications and accumulates the H3.3 histone variant, the substrate for replicat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKittrick E,Gafken PR,Ahmad K,Henikoff S

更新日期：2004-02-10 00:00:00

abstract：:The image that best expresses Darwin's thinking is the tree of life. However, Darwin's human evolutionary tree lacked almost everything because only the Neanderthals were known at the time and they were considered one extreme expression of our own species. Darwin believed that the root of the human tree was very deep ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Arsuaga JL

更新日期：2010-05-11 00:00:00

abstract：:Tropomyosin is a key component of the contractile systems found in muscle and nonmuscle cells of higher eukaryotes. Based on properties common to all tropomyosins, we have purified a protein from Saccharomyces cerevisiae that resembles tropomyosins from higher cells. The yeast protein remains soluble after heat treatm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu HP,Bretscher A

更新日期：1989-01-01 00:00:00

abstract：:The recent observation of superconductivity in [Formula: see text] has raised fundamental questions about the hierarchy of the underlying electronic structure. Calculations suggest that this system falls in the Mott-Hubbard regime, rather than the charge-transfer configuration of other nickel oxides and the supercondu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodge BH,Li D,Lee K,Osada M,Wang BY,Sawatzky GA,Hwang HY,Kourkoutis LF

更新日期：2021-01-12 00:00:00

abstract：:The body-wall muscle cells of the nematode Caenorhabditis elegans produce thick filaments during embryonic, larval, and adult stages. These thick filaments contain two myosin isoforms, A and B, which assemble into different zones along the 10-microns lengths. Paramyosin, a protein homologous to myosin rods, forms a su...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honda S,Epstein HF

更新日期：1990-02-01 00:00:00

abstract：:A model chemical network involving reactions and diffusion is studied. Spatially and temporally ordered solutions of the equations are found by bifurcation theory. These solutions are calculated analytically and their stability is studied. Properties of these dissipative structures are discussed, and a comparison with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicolis G,Auchmuty JF

更新日期：1974-07-01 00:00:00

abstract：:With every pair of bounded self-adjoint operators {U,V} on Hilbert space such that VU - UV = (1/pii)C, where C is trace class, there is associated a certain function of two complex variables called the determining function of the pair. It was previously shown how the determining function can be obtained as the solutio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pincus JD

更新日期：1971-08-01 00:00:00

abstract：:The science around the use of masks by the public to impede COVID-19 transmission is advancing rapidly. In this narrative review, we develop an analytical framework to examine mask usage, synthesizing the relevant literature to inform multiple areas: population impact, transmission characteristics, source control, wea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Howard J,Huang A,Li Z,Tufekci Z,Zdimal V,van der Westhuizen HM,von Delft A,Price A,Fridman L,Tang LH,Tang V,Watson GL,Bax CE,Shaikh R,Questier F,Hernandez D,Chu LF,Ramirez CM,Rimoin AW

更新日期：2021-01-26 00:00:00

abstract：:A method using PCR amplification and primer extension with fluorescent oligonucleotides was developed to analyze T-cell repertoires. The sizes of the hypervariable CDR3-like regions of the murine T-cell antigen receptor beta chains were measured for all possible V beta-J beta combinations. This analysis shows that bet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pannetier C,Cochet M,Darche S,Casrouge A,Zöller M,Kourilsky P

更新日期：1993-05-01 00:00:00

abstract：:The gene encoding the receptor for hyaluronan-mediated motility (RHAMM) is overexpressed in many human cancers. However, it is unclear whether RHAMM plays a causal role in tumor initiation or progression. Using somatic gene transfer in a mouse model of islet cell tumorigenesis, we demonstrate that RHAMM isoform B (RHA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du YC,Chou CK,Klimstra DS,Varmus H

更新日期：2011-10-04 00:00:00

abstract：:Dendrite morphogenesis is regulated by neuronal activity or neurotrophins, which may function by activating intrinsic signaling proteins, including Rho family GTPases. Here we report that activity- and brain-derived neurotrophic factor (BDNF)-dependent dendritic morphogenesis requires activation of geranylgeranyltrans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou XP,Wu KY,Liang B,Fu XQ,Luo ZG

更新日期：2008-11-04 00:00:00

abstract：:Superhard metals are of interest as possible replacements with enhanced properties over the metal carbides commonly used in cutting, drilling, and wear-resistant tooling. Of the superhard metals, the highest boride of tungsten--often referred to as WB4 and sometimes as W(1-x)B3--is one of the most promising candidates...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lech AT,Turner CL,Mohammadi R,Tolbert SH,Kaner RB

更新日期：2015-03-17 00:00:00

abstract：:NGC 7027 shows the richest spectrum of any known gaseous nebula. In the region of the spectrum surveyed we have measured lines of ions of H, He, C, N, O, F, Ne, Mg, Si, P, S, Cl, Ar, K, Ca, Mn, and Fe using the new Hamilton Echelle Spectrograph of Lick Observatory with a charge-coupled device (CCD) detector. Since a C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aller LH,Keyes CD

更新日期：1988-04-01 00:00:00

abstract：:Macrophage migration inhibition factor (MIF) is one of a class of lymphocyte-derived mediator substances (lymphokines) which plays a role in the mechanism of cellular immunity. A variety of other soluble factors produced by non-lymphoid cells have been shown to have effects on macrophage mobility similar to that of MI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoshida T,Bigazzi P,Cohen S

更新日期：1975-04-01 00:00:00

abstract：:An isoform of the mammalian renal type II Na/Pi-cotransporter is described. Homology of this isoform to described mammalian and nonmammalian type II cotransporters is between 57 and 75%. Based on major diversities at the C terminus, the new isoform is designed as type IIb Na/Pi-cotransporter. Na/Pi-cotransport mediate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hilfiker H,Hattenhauer O,Traebert M,Forster I,Murer H,Biber J

更新日期：1998-11-24 00:00:00

abstract：:Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease of the CNS that causes disability in young adults as a result of the irreversible accumulation of neurological deficits. Although there are potent disease-modifying agents for its initial relapsing-remitting phase, these therapies show limited...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rothhammer V,Kenison JE,Tjon E,Takenaka MC,de Lima KA,Borucki DM,Chao CC,Wilz A,Blain M,Healy L,Antel J,Quintana FJ

更新日期：2017-02-21 00:00:00

abstract：:It is well established that exposure to solar UVB (290-320 nm) gives rise to mutations in oncogenes and tumor suppressor genes that initiate the molecular cascade toward skin cancer. Although UVA (320-400 nm) has also been implicated in multistage photocarcinogenesis, its potential contribution to sunlight mutagenesis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drobetsky EA,Turcotte J,Châteauneuf A

更新日期：1995-03-14 00:00:00