Near-infrared fluorescent sensor for in vivo copper imaging in a murine Wilson disease model.

abstract：:Nascent replicative form type II (RFII) DNA of coliphage M13 synthesized in an Escherichia coli mutant deficient in the 5' leads to 3' exonuclease associated uith DNA polymerase I contains ribonucleotides that are retained in the covalently closed RFI DNA sealed in vitro by the joint action of T5 phage DNA polymerase ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dasgupta S,Mitra S

更新日期：1978-01-01 00:00:00

abstract：:Epithelial barrier disruption is a major cause of inflammatory bowel disease (IBD); however, the cellular and molecular regulation of intestinal epithelial homeostasis remains largely undefined. Here, we show that the C-type lectin receptor LSECtin (Clec4g) on macrophages is required for protection against dextran sul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Z,Li Q,Wang X,Jiang X,Zhao D,Lin X,He F,Tang L

更新日期：2018-10-23 00:00:00

abstract：:The difficulty of achieving robust functional expression of insect nicotinic acetylcholine receptors (nAChRs) has hampered our understanding of these important molecular targets of globally deployed neonicotinoid insecticides at a time when concerns have grown regarding the toxicity of this chemotype to insect pollina...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ihara M,Furutani S,Shigetou S,Shimada S,Niki K,Komori Y,Kamiya M,Koizumi W,Magara L,Hikida M,Noguchi A,Okuhara D,Yoshinari Y,Kondo S,Tanimoto H,Niwa R,Sattelle DB,Matsuda K

更新日期：2020-07-14 00:00:00

abstract：:The c-myc protooncogene has been implicated in control of growth and differentiation of mammalian cells. For instance, growth arrest is often preceded by reduction in c-myc mRNA and gene transcription. To elucidate the mechanisms of control of c-myc gene transcription, we have begun to characterize the interaction of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duyao MP,Buckler AJ,Sonenshein GE

更新日期：1990-06-01 00:00:00

abstract：:Alzheimer's disease is characterized by two primary pathological features: amyloid plaques and neurofibrillary tangles. The interconnection between amyloid and tau aggregates is of intense interest, but mouse models have yet to reveal a direct interrelationship. We now show that NO may be a key factor that connects am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colton CA,Vitek MP,Wink DA,Xu Q,Cantillana V,Previti ML,Van Nostrand WE,Weinberg JB,Dawson H

更新日期：2006-08-22 00:00:00

abstract：:We have isolated a protein from Saccharomyces cerevisiae that binds specifically to a nucleotide sequence associated with the autonomously replicating sequence (ARS) ARS120, located in the telomeric region of a yeast chromosome. "Footprinting" analysis revealed that a 26-base-pair DNA sequence, 5'-CAAGTGCCGTGCATAATGAT...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eisenberg S,Civalier C,Tye BK

更新日期：1988-02-01 00:00:00

abstract：:For a distribution F on R(p) and a point x in R(p) the simplicial depth D(x), which is the probability that x be inside a random simplex whose vertices are p + 1 independent observations from F, is introduced. D(x) can be viewed as a measure of depth of the point x relative to F, and its empirical version gives rise t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu RY

更新日期：1988-03-01 00:00:00

abstract：:We investigate the hypothesis that two classes of Ca2+ currents, one quickly inactivated by Ca2+ and one slowly inactivated by voltage, contribute to bursting electrical activity in pancreatic islets. A mathematical model of these currents is fit to the experimental whole-cell current-voltage and inactivation profiles...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keizer J,Smolen P

更新日期：1991-05-01 00:00:00

abstract：:Campylobacter jejuni has a general N-linked glycosylation pathway (encoded by the pgl gene cluster), which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac [where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glover KJ,Weerapana E,Imperiali B

更新日期：2005-10-04 00:00:00

abstract：:Notch (N) is a transmembrane receptor that mediates cell-cell interactions to determine many cell-fate decisions. N contains EGF-like repeats, many of which have an O-fucose glycan modification that regulates N-ligand binding. This modification requires GDP-L-fucose as a donor of fucose. The GDP-L-fucose biosynthetic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ayukawa T,Matsumoto K,Ishikawa HO,Ishio A,Yamakawa T,Aoyama N,Suzuki T,Matsuno K

更新日期：2012-09-18 00:00:00

abstract：:Viral glycoproteins mediate entry of enveloped viruses into cells and thus play crucial roles in infection. In herpesviruses, a complex of two viral glycoproteins, gH and gL (gH/gL), regulates membrane fusion events and influences virion cell tropism. Human cytomegalovirus (HCMV) gH/gL can be incorporated into two dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li G,Nguyen CC,Ryckman BJ,Britt WJ,Kamil JP

更新日期：2015-04-07 00:00:00

abstract：:Cells contain numerous enzymes that use molecular oxygen for their reactions. Often, their active sites are buried deeply inside the protein, which raises the question whether there are specific access channels guiding oxygen to the site of catalysis. Choosing 12/15-lipoxygenase as a typical example for such oxygen-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saam J,Ivanov I,Walther M,Holzhütter HG,Kuhn H

更新日期：2007-08-14 00:00:00

abstract：:Responses of tethered cells of Escherichia coli to impulse, step, exponential-ramp or exponentiated sine-wave stimuli are internally consistent, provided that allowance is made for the nonlinear effect of thresholds. This result confirms that wild-type cells exposed to stimuli in the physiological range make short-ter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Segall JE,Block SM,Berg HC

更新日期：1986-12-01 00:00:00

abstract：:Protein tyrosine phosphorylation and dephosphorylation are key regulatory events in T-cell receptor (TCR) signaling. We investigated the role of the tyrosine phosphatase SHPTP1 in TCR signaling by analysis of TCR signal transduction in motheaten (me/me) mice, which lack SHPTP1 expression. As revealed by flow cytometri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lorenz U,Ravichandran KS,Burakoff SJ,Neel BG

更新日期：1996-09-03 00:00:00

abstract：:Inside-out erythrocyte membranes attached to polycationic beads manifested glutathione disulfide (GSSG)-stimulated ATPase activity. A Lineweaver-Burk plot of the ATPase activity as a function of GSSG concentration was biphasic and gave apparent Km values of 0.13 mM and 2.0 mM. These kinetics are similar to those repor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kondo T,Kawakami Y,Taniguchi N,Beutler E

更新日期：1987-11-01 00:00:00

abstract：:The output of the cerebellar cortex is controlled by two main inputs, (i.e., the climbing fiber and mossy fiber-parallel fiber pathway) and activations of these inputs elicit characteristic effects in its Purkinje cells: that is, the so-called complex spikes and simple spikes. Target neurons of the Purkinje cells in t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoebeek FE,Witter L,Ruigrok TJ,De Zeeuw CI

更新日期：2010-05-04 00:00:00

abstract：:The complete coding region of maize transposable element Ac and truncated but active derivatives of it were placed under the control of promoters of different strength and tested for the ability to excise transposable element Ds from a beta-glucuronidase reporter gene in a cotransfection assay in Petunia protoplasts. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Becker D,Lütticke R,Li M,Starlinger P

更新日期：1992-06-15 00:00:00

abstract：:Molecular dynamics simulations of the response to oscillating electric field elicited from an altitudinal dipolar molecular rotor mounted on the Au(111) surface and previously studied experimentally in static fields show unidirectional rotation in one of the three pairs of conformational enantiomers. The simulations a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horinek D,Michl J

更新日期：2005-10-04 00:00:00

abstract：:Nanos (Nos) is an evolutionarily conserved protein essential for the survival of primordial germ cells. In Drosophila, maternal Nos partitions into pole cells and suppresses apoptosis to permit proper germ-line development. However, how this critical event is regulated by Nos has remained elusive. Here, we report that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sato K,Hayashi Y,Ninomiya Y,Shigenobu S,Arita K,Mukai M,Kobayashi S

更新日期：2007-05-01 00:00:00

abstract：:Discrete state models are a common tool of modeling in many areas. E.g., Markov state models as a particular representative of this model family became one of the major instruments for analysis and understanding of processes in molecular dynamics (MD). Here we extend the scope of discrete state models to the case of s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerber S,Horenko I

更新日期：2014-10-14 00:00:00

abstract：:The S1-nuclease analysis was used to investigate the pattern of inhibin expression in the rat. In a first series of experiments, expression of the alpha, beta A, and beta B subunits of inhibin were monitored in various tissues from male and female rats. Two observations emerge from these studies. First, expression of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meunier H,Rivier C,Evans RM,Vale W

更新日期：1988-01-01 00:00:00

abstract：:The MHC class I antigen presentation pathway allows the immune system to distinguish between self and nonself. Despite extensive research on the processing of antigenic peptides, little is known about their origin. Here, we show that mRNAs carrying premature stop codons that prevent the production of full-length prote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Apcher S,Daskalogianni C,Lejeune F,Manoury B,Imhoos G,Heslop L,Fåhraeus R

更新日期：2011-07-12 00:00:00

abstract：:RNA viruses are notorious for their genetic plasticity and propensity to exploit new host-range opportunities, which can lead to the emergence of human disease epidemics such as severe acute respiratory syndrome, AIDS, dengue, and influenza. However, the mechanisms of host-range change involved in most of these viral ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anishchenko M,Bowen RA,Paessler S,Austgen L,Greene IP,Weaver SC

更新日期：2006-03-28 00:00:00

abstract：:Antibody-coated latex beads (Ab-beads) were attached to Leptospira interrogans serovars illini 3055 and icterohaemorrhagiae SC1157. The movement of the Ab-beads relative to the motion of the cells was observed by direct darkfield microscopy or was recorded on videotape. When the Ab-beads were attached to the front end...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Charon NW,Lawrence CW,O'Brien S

更新日期：1981-11-01 00:00:00

abstract：:The detection, stabilization, and repair of stress-induced damage are essential requirements for cellular life. All cells respond to osmotic stress-induced water loss with increased expression of genes that mediate accumulation of organic osmolytes, solutes that function as chemical chaperones and restore osmotic home...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lamitina T,Huang CG,Strange K

更新日期：2006-08-08 00:00:00

abstract：:A methylcholanthrene-induced glioblastoma of the C57BL/6 inbred mouse strain was used to raise antibodies in C57BL/6 and C57BL/10 inbred mice and in (C57BL/6 x DBA/2) and (C57BL/6 x Balb/c) F(1) hybrids. When examined by the cytotoxicity test, these antibodies define a cell-surface component (or components) found excl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schachner M

更新日期：1974-05-01 00:00:00

abstract：:In 1000 primary gastric carcinomas, 70 (7.0%) contained Epstein-Barr virus (EBV) genomic sequences detected by PCR and Southern blots. The positive tumors comprised 8 of 9 (89%) undifferentiated lymphoepithelioma-like carcinomas, 27 of 476 (5.7%) poorly differentiated adenocarcinomas, and 35 of 515 (6.8%) moderately t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Imai S,Koizumi S,Sugiura M,Tokunaga M,Uemura Y,Yamamoto N,Tanaka S,Sato E,Osato T

更新日期：1994-09-13 00:00:00

abstract：:To successfully colonize the human gut, enteric bacteria must activate acid resistance systems to survive the extreme acidity (pH 1.5-3.5) of the stomach. The antiporter AdiC is the master orchestrator of the arginine-dependent system. Upon acid shock, it imports extracellular arginine (Arg) into the cytoplasm, provid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai MF,Miller C

更新日期：2013-04-09 00:00:00

abstract：:Reproductive success is widely used as a measure of fitness. However, offspring quantity may not reflect the genetic contribution to subsequent generations if there is nonrandom variation in offspring quality. Offspring quality is likely to be an important component of human fitness, and tradeoffs between offspring qu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zietsch BP,Kuja-Halkola R,Walum H,Verweij KJ

更新日期：2014-01-21 00:00:00

abstract：:In previous studies from this laboratory, the binding regions of alpha-neurotoxins on human and Torpedo acetylcholine (AcCho) receptors (AcChoRs) and the binding regions for the receptor on the toxin were characterized with synthetic peptides of the respective molecules. In the present work, peptides representing the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruan KH,Spurlino J,Quiocho FA,Atassi MZ

更新日期：1990-08-01 00:00:00