Abstract:
:Global biological datasets generated by genomics, transcriptomics, and proteomics provide new approaches to understanding the relationship between the genome and the synapse. Combined transcriptome analysis and multielectrode recordings of neuronal network activity were used in mouse embryonic primary neuronal cultures to examine synapse formation and activity-dependent gene regulation. Evidence for a coordinated gene expression program for assembly of synapses was observed in the expression of 642 genes encoding postsynaptic and plasticity proteins. This synaptogenesis gene expression program preceded protein expression of synapse markers and onset of spiking activity. Continued expression was followed by maturation of morphology and electrical neuronal networks, which was then followed by the expression of activity-dependent genes. Thus, two distinct sequentially active gene expression programs underlie the genomic programs of synapse function.
journal_name
Proc Natl Acad Sci U S Aauthors
Valor LM,Charlesworth P,Humphreys L,Anderson CN,Grant SGdoi
10.1073/pnas.0609071104subject
Has Abstractpub_date
2007-03-13 00:00:00pages
4658-63issue
11eissn
0027-8424issn
1091-6490pii
0609071104journal_volume
104pub_type
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