Disassembly of the Staphylococcus aureus hibernating 100S ribosome by an evolutionarily conserved GTPase.

abstract：:Integrin alphanubeta3 is found on a subset of tumor blood vessels where it is associated with angiogenesis and malignant tumor growth. We designed an alphanubeta3-targeted nanoparticle (NP) encapsulating the cytotoxic drug doxorubicin (Dox) for targeted drug delivery to the alphanubeta3-expressing tumor vasculature. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murphy EA,Majeti BK,Barnes LA,Makale M,Weis SM,Lutu-Fuga K,Wrasidlo W,Cheresh DA

更新日期：2008-07-08 00:00:00

abstract：:The polypeptide chain initiation factor 2 (eIF-2) binds phospholipid (PL) and becomes a potent inhibitor of translation in hemin-supplemented reticulocyte lysates. This binding is markedly reduced by prior treatment of eIF-2 with N-ethylmaleimide. Although PL is probably bound by all three eIF-2 subunits, our results ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Haro C,de Herreros AG,Ochoa S

更新日期：1986-09-01 00:00:00

abstract：:The rat brain-derived neurotropic factor (BDNF) gene consists of four 5' exons linked to separate promoters and one 3' exon encoding the prepro-BDNF protein. To gain insights into the regulation of BDNF mRNA expression, probes specific for the different 5' exons were used to study the expression of BDNF mRNA in the br...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Metsis M,Timmusk T,Arenas E,Persson H

更新日期：1993-10-01 00:00:00

abstract：:Serum provides growth factors that regulate and limit the growth of normal cells in tissue culture. Animal cells that are malignantly transformed usually exhibit diminished serum requirements for growth in culture. We have used a defined, serum-free medium to determine which of these growth factors becomes dispensable...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cherington PV,Smith BL,Pardee AB

更新日期：1979-08-01 00:00:00

abstract：:Biological organisms have evolved a wide range of immune mechanisms to defend themselves against pathogens. Beyond molecular details, these mechanisms differ in how protection is acquired, processed, and passed on to subsequent generations-differences that may be essential to long-term survival. Here, we introduce a m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mayer A,Mora T,Rivoire O,Walczak AM

更新日期：2016-08-02 00:00:00

abstract：:Using morphometrical methods, we investigated variation in the skeletons of more than 400 offspring of C57BL/6J and DBA/2J male mice that had received either a 250 mg/kg dose of the mutagen ethylnitrosourea or a solvent control. Sperm involved in the matings developed from cells in the spermatogonial stage at the time...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lovell DP,Willis DB,Johnson FM

更新日期：1985-05-01 00:00:00

abstract：:Reducing monosaccharides and oligosaccharides, after reductive amination, were separated and detected as their 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde (CBQCA) derivatives by capillary electrophoresis/laser-induced fluorescence. Under optimized conditions, the minimum detectable quantities for monosaccharide sol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu JP,Shirota O,Wiesler D,Novotny M

更新日期：1991-03-15 00:00:00

abstract：:Stimulation of antitumor immune mechanisms is the primary goal of cancer immunotherapy, and accumulating evidence suggests that effective alteration of the host-tumor relationship involves immunomodulating cytokines and also the presence of costimulatory molecules. To examine the antitumor effect of direct in vivo gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pützer BM,Hitt M,Muller WJ,Emtage P,Gauldie J,Graham FL

更新日期：1997-09-30 00:00:00

abstract：:The qualities of perception depend not only on the sensory inputs but also on the brain state before stimulus presentation. Although the collective evidence from neuroimaging studies for a relation between prestimulus state and perception is strong, the interpretation in the context of sensory computations or decision...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kayser SJ,McNair SW,Kayser C

更新日期：2016-04-26 00:00:00

abstract：:Recently, a 29-residue cyclic peptide was synthesized (TrPepz) that was reported to possess nearly the same catalytic activity and specificity as the pancreatic serine protease, trypsin, for hydrolysis of a small ester substrate, N-tosyl-L-arginine methyl ester (TAME), and small and large peptides [Atassi, M. Z. & Man...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wells JA,Fairbrother WJ,Otlewski J,Laskowski M Jr,Burnier J

更新日期：1994-05-10 00:00:00

abstract：:Bacterial capsules are surface layers made of long-chain polysaccharides. They are anchored to the outer membrane of many Gram-negative bacteria, including pathogens such as Escherichia coli, Neisseria meningitidis, Haemophilus influenzae, and Pasteurella multocida. Capsules protect pathogens from host defenses includ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willis LM,Stupak J,Richards MR,Lowary TL,Li J,Whitfield C

更新日期：2013-05-07 00:00:00

abstract：:Ca2+ ions control the cGMP-gated channel of rod photoreceptor cells from the external and internal face. We studied ion selectivity and blockage by Ca2+ of wild-type and mutant channels in a heterologous expression system. External Ca2+ blocks the inward current at micromolar concentrations in a highly voltage-depende...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eismann E,Müller F,Heinemann SH,Kaupp UB

更新日期：1994-02-01 00:00:00

abstract：:The sodium/iodide symporter (NIS) mediates active I(-) transport in the thyroid-the first step in thyroid hormone biosynthesis-with a 2 Na(+): 1 I(-) stoichiometry. The two Na(+) binding sites (Na1 and Na2) and the I(-) binding site interact allosterically: when Na(+) binds to a Na(+) site, the affinity of NIS for the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrandino G,Nicola JP,Sánchez YE,Echeverria I,Liu Y,Amzel LM,Carrasco N

更新日期：2016-09-13 00:00:00

abstract：:In Arabidopsis, the two-dimensional expansion of leaves is regulated via the polarized elongation of cells. The ROTUNDIFOLIA3 (ROT3) protein, a member of the family of cytochromes P450, is involved in this process and regulates leaf length. Transgenic plants that overexpressed a wild-type ROT3 gene had longer leaves t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim GT,Tsukaya H,Saito Y,Uchimiya H

更新日期：1999-08-03 00:00:00

abstract：:The midcycle luteinizing hormone (LH) surge triggers several tightly linked ovarian processes, including steroidogenesis, oocyte maturation, and ovulation. We designed studies to determine whether epidermal growth factor receptor (EGFR)-mediated signaling might serve as a common regulator of these activities. Our resu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamnongjit M,Gill A,Hammes SR

更新日期：2005-11-08 00:00:00

abstract：:Mutants of Escherichia coli sensitive to phage T4 gene 2 mutants were obtained following ethyl methanesulfonate mutagenesis. By mapping and complementation analysis, the mutations in each of the six mutants are in recB and recC. By both in vivo and in vitro analyses, the nuclease activity of RecBC enzyme is undetectab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaudhury AM,Smith GR

更新日期：1984-12-01 00:00:00

abstract：:By patch-clamp analysis, we have shown that chronic, intermittent mechanical strain (CMS) increases the activity of stretch-activated cation channels of osteoblast-like UMR-106.01 cells. CMS also produces a swelling-activated whole-cell conductance (Gm) regulated by varying strain levels. We questioned whether the swe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duncan RL,Kizer N,Barry EL,Friedman PA,Hruska KA

更新日期：1996-03-05 00:00:00

abstract：:Current models of the formation and distribution of gold deposits on Earth are based on the long-standing paradigm that hydrogen sulfide and chloride are the ligands responsible for gold mobilization and precipitation by fluids across the lithosphere. Here we challenge this view by demonstrating, using in situ X-ray a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pokrovski GS,Kokh MA,Guillaume D,Borisova AY,Gisquet P,Hazemann JL,Lahera E,Del Net W,Proux O,Testemale D,Haigis V,Jonchière R,Seitsonen AP,Ferlat G,Vuilleumier R,Saitta AM,Boiron MC,Dubessy J

更新日期：2015-11-03 00:00:00

abstract：:AvrXa7 is a member of the avrBs3 avirulence gene family, which encodes proteins targeted to plant cells by a type III secretion apparatus. AvrXa7, the product of avrXa7, is also a virulence factor in strain PXO86 of Xanthomonas oryzae pv. oryzae. Avirulence and virulence specificities are associated with the central r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang B,Zhu W,Johnson LB,White FF

更新日期：2000-08-15 00:00:00

abstract：:Artificial two-dimensional biological habitats were prepared from porous polymer layers and inoculated with the fungus Penicillium roqueforti to provide a living material. Such composites of classical industrial ingredients and living microorganisms can provide a novel form of functional or smart materials with capabi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerber LC,Koehler FM,Grass RN,Stark WJ

更新日期：2012-01-03 00:00:00

abstract：:Intracisternal A-particles were isolated from three different myeloma lines in BALB/c mice and from cultured neuroblastoma cells of A/J origin. All preparations contained a major structural protein with an apparent molecular weight near 70,000 as estimated by electrophoretic mobility in sodium dodecyl sulfate-containi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kuff EL,Leuders KK,Ozer HL,Wivel NA

更新日期：1972-01-01 00:00:00

abstract：:Well-differentiated liposarcoma (WDLPS), one of the most common human sarcomas, is poorly responsive to radiation and chemotherapy, and the lack of animal models suitable for experimental analysis has seriously impeded functional investigation of its pathobiology and development of effective targeted therapies. Here, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gutierrez A,Snyder EL,Marino-Enriquez A,Zhang YX,Sioletic S,Kozakewich E,Grebliunaite R,Ou WB,Sicinska E,Raut CP,Demetri GD,Perez-Atayde AR,Wagner AJ,Fletcher JA,Fletcher CD,Look AT

更新日期：2011-09-27 00:00:00

abstract：:Treatment of cultured human tumor cells with the chloroethylnitrosourea antitumor drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) selectively induces transcription and protein synthesis of a subset of the human heat shock or stress-induced genes (HSP90 and HSP70) with little effect on other stress genes or on express...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kroes RA,Abravaya K,Seidenfeld J,Morimoto RI

更新日期：1991-06-01 00:00:00

abstract：:The stress inoculation hypothesis presupposes that brief intermittent stress exposure early in life induces the development of subsequent stress resistance in human and nonhuman primates. Rodent studies, however, suggest a role for maternal care rather than stress exposure per se (i.e., the maternal mediation hypothes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker KJ,Buckmaster CL,Sundlass K,Schatzberg AF,Lyons DM

更新日期：2006-02-21 00:00:00

abstract：:The generation of high-titer, helper-free retroviruses by transient transfection has been achieved by using the highly transfectable 293T cell line into which are stably introduced constructs that express retroviral packaging functions. The resulting ecotropic virus packaging cell line BOSC 23 produces infectious retr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pear WS,Nolan GP,Scott ML,Baltimore D

更新日期：1993-09-15 00:00:00

abstract：:Mate attraction in Aplysia involves a long-distance water-borne signal (the protein pheromone attractin), which is released during egg laying. Aplysia californica attractin attracts species that produce closely related attractins, such as Aplysia brasiliana, whose geographic distribution does not overlap that of A. ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Painter SD,Cummins SF,Nichols AE,Akalal DB,Schein CH,Braun W,Smith JS,Susswein AJ,Levy M,de Boer PA,ter Maat A,Miller MW,Scanlan C,Milberg RM,Sweedler JV,Nagle GT

更新日期：2004-05-04 00:00:00

abstract：:Leukosialin (also known as Ly48, CD43, and sialophorin) is a major cell surface sialoglycoprotein found on a variety of hematopoietically derived cells. The precise function of this molecule is poorly understood but it has been implicated in cell proliferation and intercellular adhesion. We developed a transgenic mous...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dragone LL,Barth RK,Sitar KL,Disbrow GL,Frelinger JG

更新日期：1995-01-17 00:00:00

abstract：:Understanding the mechanisms by which compounds discovered using cell-based phenotypic screening strategies might exert their effects would be highly augmented by new approaches exploring their potential interactions with the genome. For example, altered androgen receptor (AR) transcriptional programs, including castr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin C,Yang L,Xie M,Lin C,Merkurjev D,Yang JC,Tanasa B,Oh S,Zhang J,Ohgi KA,Zhou H,Li W,Evans CP,Ding S,Rosenfeld MG

更新日期：2014-06-24 00:00:00

abstract：:We have initiated immunological and drug-binding studies on the tubulins from different higher plant species. Antibodies were raised against electrophoretically separated rose (Rosa sp.) tubulin alpha- and beta-subunits and characterized by immunoblot autoradiographic assays. Each IgG preparation bound to its antigen ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morejohn LC,Bureau TE,Tocchi LP,Fosket DE

更新日期：1984-03-01 00:00:00

abstract：:Synthesis of normal human hemoglobin A, alpha 2 beta 2, is based upon balanced expression of genes in the alpha-globin gene cluster on chromosome 16 and the beta-globin gene cluster on chromosome 11. Full levels of erythroid-specific activation of the beta-globin cluster depend on sequences located at a considerable d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liebhaber SA,Griese EU,Weiss I,Cash FE,Ayyub H,Higgs DR,Horst J

更新日期：1990-12-01 00:00:00