Abstract:
:Nicotine is the major addictive compound in tobacco and is responsible for tobacco dependence. It is primarily metabolized to cotinine (COT) and trans-3'-hydroxycotinine (3HC) by the liver enzyme cytochrome P-450 2A6 (CYP2A6). The 3HC/COT ratio measured in the saliva of smokers is highly correlated with the intrinsic hepatic clearance of nicotine and, therefore, may be a useful non-invasive marker of CYP2A6 activity and metabolic rate of nicotine. This study assessed within-subject variation in salivary 3HC/COT ratios in six regular daily smokers. Our data provide evidence that 1. variation in the 3HC/COT ratio is not dependent on the time of sampling during the day (i.e., morning vs. night ) (P > 0.1) and 2. the average within-subject biological variation in the 3HC/COT ratio is approximately 26%. These findings should be useful for designing large-scale population surveys to assess the variation in the metabolic rate of nicotine (via CYP2A6) in smokers.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Lea RA,Dickson S,Benowitz NLdoi
10.1093/jat/30.6.386subject
Has Abstractpub_date
2006-07-01 00:00:00pages
386-9issue
6eissn
0146-4760issn
1945-2403journal_volume
30pub_type
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of analytical toxicology
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journal_title:Journal of analytical toxicology
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