Abstract：
:Particle tracking velocimetry provides a Lagrangian description of flow properties in the microcirculation. To determine the utility of fluorescent nanoparticles to provide Lagrangian coordinates, we tracked these particles both in vitro and in vivo. The particles had a neutral charge and fluorescence intensity greater than 1,000 times the PKH26-labeled red blood cells. At image acquisition rates of 60 frames per second, particles were tracked at velocities up to 4,000 microm/s. Morphometric changes reflecting streaking artifact were significant at velocities of 4,000 microm/s (P < 0.05), but not at lower velocities (P > 0.05). Intravital microscopy monitoring after intravenous injection of the particles demonstrated a circulation half-life that was inversely related to particle size: 500 nm nanoparticles demonstrated a smaller change in plasma concentration than larger particles. Regardless of the size of the particles, more than 50% of the recovered fluorescence was located in the liver. These results suggest that fluorescent nanoparticles provide a convenient and practical Lagrangian description of flow velocity in the microcirculation.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Ravnic DJ,Zhang YZ,Tsuda A,Pratt JP,Huss HT,Mentzer SJdoi
10.1016/j.mvr.2006.04.006subject
Has Abstractpub_date
2006-07-01 00:00:00pages
27-33issue
1-2eissn
0026-2862issn
1095-9319pii
S0026-2862(06)00045-8journal_volume
72pub_type
杂志文章abstract：:Red blood cell (RBC)-plasma suspensions with a hematocrit of 10-12% were forced to flow through microchannels (equivalent diameter 6 microns, equivalent length 20 microns, 2600 in parallel) by applying a pressure difference of 20 cm H2O. Transit times for 100 microliters of fresh suspensions from 33 healthy male subje...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1017
更新日期：1994-03-01 00:00:00
abstract：:The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.05.001
更新日期：2004-09-01 00:00:00
abstract：UNLABELLED:Metabolic syndrome (MetS) increases the risk of the subsequent development of cardiovascular disease. This study aimed to determine if the harmonic indexes of finger photoplethysmography (PPG) waveforms can be used to discriminate different arterial pulse transmission conditions between MetS and healthy subj...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.04.001
更新日期：2016-07-01 00:00:00
abstract：:Placental development requires coordinated angiogenesis regulated by multiple factors including angiopoietins. Previously we demonstrated that the concentration of angiopoietin-2 (Ang-2) in the sera of women rises markedly in pregnancy in early gestation. This increase is reduced in pregnancies subsequently complicate...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.08.003
更新日期：2012-11-01 00:00:00
abstract：:Synthetic big endothelin-1 (ET-1), a 39-residue precursor of ET-1, has been reported to elicit potent contractile action on helical strip specimens obtained from the porcine coronary artery, but its molar potency was found to be 140-fold lower than that of ET-1 [Saito, Y., Nakao, K., Mukoyama, M., Imura, H., 1990. Inc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.04.007
更新日期：2006-11-01 00:00:00
abstract：:A study was designed to compare three independent measures of cutaneous blood flow in normal healthy volunteers: xenon-133 washout, helium flux, and laser velocimetry. All measurements were confined to the volar aspect of the forearm. In a large group of subjects we found that helium flux through intact skin changes n...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(88)90058-1
更新日期：1988-03-01 00:00:00
abstract：OBJECTIVE:High aerobic fitness may prevent age-related decrements in cutaneous vasodilation while type 2 diabetes may exacerbate this decline. The mechanisms underlying these responses remain unclear, but may be due to an excess of reactive oxygen species. We hypothesized that superoxide scavenging or NADPH oxidase inh...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103886
更新日期：2019-09-01 00:00:00
abstract：:Despite intensive study in the area of angiogenesis, relatively little is known about normal angiogenesis in adult animals. Preliminary studies using the Griffonia simplicifolia I (GSI) lectin as a microvascular marker indicated that capillary sprouting occurs in the clear "windows" of normal intact adult rat mesenter...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1029
更新日期：1994-05-01 00:00:00
abstract：:The key points in the design of microfluidic Lab-On-a-Chips for blood tests are the simplicity of the microfluidic chip geometry, the portability of the monitoring system and the ease on-chip integration of the data analysis procedure. The majority of those, recently designed, have been used for blood separation, howe...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.09.003
更新日期：2018-03-01 00:00:00
abstract：:Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascul...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.03.001
更新日期：2010-07-01 00:00:00
abstract：:The aim of this study was to evaluate whether streptokinase (SK) may produce beneficial effects at the level of microvascular circulation in addition to coronary recanalization. Twenty mongrel dogs weighing 28.4 +/- 4.6 kg were randomized to receive SK (16,000-72,000 U/kg) or 20 ml saline (control) in an open-chest an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2236
更新日期：2000-07-01 00:00:00
abstract：:Techniques of intravital microscopy were used to assess the effect of the nonsteroidal anti-inflammatory drugs (NSAIDs), indomethacin and ibuprofen, on the microcirculation. Hemodynamics in venules of the rat mesentery were studied in terms of vessel diameter, red blood cell velocity, and leukocyte-endothelium interac...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1016
更新日期：1993-03-01 00:00:00
abstract：:Nitrate supplementation in the form of beetroot juice has been shown to increase nitric oxide (NO) where nitrate can be reduced to nitrite and, subsequently, to NO through both nitric oxide synthase (NOS)-dependent and -independent pathways. We tested the hypothesis that nitrate supplementation would augment the NO co...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.12.002
更新日期：2015-03-01 00:00:00
abstract：:Hypoxia-induced arginase elevation plays an essential role in several vascular diseases but influence of arginase on hypoxia-mediated angiogenesis is completely unknown. In this study, in vitro network formation in bovine aortic endothelial cells (BAEC) was examined after exposure to hypoxia for 24h with or without ar...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.11.002
更新日期：2015-03-01 00:00:00
abstract：OBJECTIVES:Calcium channel inhibitors have beneficial impact on microcirculation, but beta-blocker effect is controversial. Clinicians still do not agree on beta-blocker combination with other treatments in the management of impaired microcirculation. The aim of the present study was to describe the effects of beta-blo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2011.04.004
更新日期：2011-07-01 00:00:00
abstract：:Liposomally formulated siRNA can be used for RNAi applications in vivo. Intravenous bolus administration of lipoplexed siRNA has been shown to reduce gene expression in the vascular endothelium. Here, we applied immunofluorescence staining for different endothelial markers (PECAM-1, CD34, laminin) on paraffin sections...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.02.004
更新日期：2008-05-01 00:00:00
abstract：:Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and con...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.011
更新日期：2012-11-01 00:00:00
abstract：:A well developed capillary bed is essential for proper function of skeletal muscles. We present for the first time a triple immunofluorescent method suitable for staining capillaries and muscle fibre outlines in thick sections of human skeletal muscle, applying antibodies against collagen IV (in red) and F8 (in green)...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.11.012
更新日期：2011-03-01 00:00:00
abstract：:We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title：Microvascular research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:Fluid homeostasis is required for life. Processes involved in fluid balance are strongly related to exchanges at the microvascular level. Computational models have been presented in the literature to analyze the microvascular-interstitial interactions. As far as we know, none of those models consider a physiological d...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.11.003
更新日期：2019-03-01 00:00:00
abstract：:Arterioles in various vascular beds have been often observed to have nonuniform diameters along the vessel axis. The effect of nonuniformity of arteriolar diameter on vascular resistance was investigated using a theoretical model of blood flow in arterioles. Viscous flow of a Newtonian fluid in tubes with periodically...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(89)90023-x
更新日期：1989-09-01 00:00:00
abstract：:The purpose of this study was to investigate reactive hyperemia in the capillary network of human skin in terms of the flow per capillary and the density of flow-active capillaries. Seventeen male subjects 20 to 40 years of age were seated with their right foot placed on the stage of a Leitz epi-ilumination microscope...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(85)90040-8
更新日期：1985-07-01 00:00:00
abstract：:The kidneys of 14 female Wistar rats from 5 days to 18 months postnatally have been perfusion-fixed to study the development of glomerular capillaries using design-based stereological techniques. This report uses a topological definition of a capillary structural unit which forms the basis for the estimation of the nu...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1022
更新日期：1993-05-01 00:00:00
abstract：BACKGROUND:The proepicardium (PE), a transient embryonic structure crucial for the development of the epicardium and heart, contains its own population of endothelial cells (ECs). The aim of our study was to determine the pattern, anatomical orientation and phenotypic marker expression of the endothelial cell network w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.08.007
更新日期：2015-11-01 00:00:00
abstract：:We previously reported that the cutaneous vasodilator response to local warming in males required noradrenaline (NA) and neuropeptide-Y (NPY). Animal work has shown no role for NPY in female vascular control. We investigated the contribution of NA and NPY in human female cutaneous vascular control. Nine female and nin...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2017.04.003
更新日期：2017-09-01 00:00:00
abstract：:Cardiovascular diseases, among all diseases, are taking the most victims worldwide. Coronary artery occlusion, takes responsibility of about 30% of the yearly global deaths in the world (Heart Disease and Stroke Statistics 2017 At-a-Glance, 2017), raising the need for viable substitutes for cardiovascular tissues. Dep...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2020.104027
更新日期：2020-09-01 00:00:00
abstract：:A mathematical model of nonsteady-state fluid movement in the lung interstitium during hydrodynamic loading is evaluated. The model is based on experimental results obtained in isolated perfused canine lungs. The interstitium is treated as a porous phase with an elastic framework, able to relax; all resistance to flux...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90071-i
更新日期：1991-07-01 00:00:00
abstract：:Although microvascular dysfunction (MVD) has been well characterized in individual organs as different disease entities, clinical evidence is mounting in support of an underlying systemic process. To address this hypothesis, we systematically searched PubMed and Medline for studies in adults published between 2014 and...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2020.104123
更新日期：2021-03-01 00:00:00
abstract：:Pulmonary arterial hypertension is a common complication of congenital heart defects with left-to-right shunts. Current preclinical models do not reproduce clinical characteristics of shunt-related pulmonary hypertension. Aorto-caval shunt was firstly described as a model of right ventricle volume overload. The pathop...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.005
更新日期：2014-07-01 00:00:00
abstract：:In this paper we quantitatively investigate the hypothesis proposed by Michel (Exp. Physiol. 82, 1-30, 1997) and Weinbaum (Ann. Biomed. Eng. 26, 1-17, 1998) that the Starling forces are determined by the local difference in the hydrostatic and colloid osmotic pressure across the endothelial surface glycocalyx, which w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1999.2177
更新日期：1999-11-01 00:00:00
