Abstract：
:Leukemic bcr-abl positive dendritic cells (DCs) are likely to be present in vivo in chronic myelogenous leukemia (CML) patients, but no data are available on their functional qualities. We analyzed the circulating BDCA-1+ myeloid DC compartment in 15 chronic phase CML patients. Phenotypic features of CML DCs were comparable with that of normal DCs, except for the CD80 and CD40 antigens, significantly under-represented in CML patients. Nonetheless, no differences were found between normal samples and leukemic DCs in the allostimulatory ability, as well as in the production of cytokines and polarization of T cell responses. CML DCs were analyzed by fluorescence in situ hybridization (FISH) and found positive for the bcr-abl translocation. However, when bcr-abl+ DCs were tested for their ability to stimulate an autologous T-cell response in vitro, we could not detect a specific recognition. We conclude that an apparently normal circulating DC compartment carrying the Ph+ chromosome can be identified in CML patients; however, these cells appear unable to trigger a protective anti-leukemic immune response in autologous T cells.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Orsini E,Calabrese E,Maggio R,Pasquale A,Nanni M,Trasarti S,Tafuri A,Guarini A,Foa Rdoi
10.1016/j.leukres.2005.11.028keywords:
subject
Has Abstractpub_date
2006-07-01 00:00:00pages
785-94issue
7eissn
0145-2126issn
1873-5835pii
S0145-2126(05)00469-8journal_volume
30pub_type
杂志文章abstract：:A 53-year-old male patient was admitted to our hospital with painful splenomegaly. He was diagnosed as having chronic myelomonocytic leukemia (CMML) with leukocytosis, monocytosis, increased lysozyme concentrations in serum und urine, and lack of the Philadelphia chromosome. The clinical course of the disease was char...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(96)00117-8
更新日期：1997-03-01 00:00:00
abstract：:The impact of socioeconomic status (SES) upon childhood cancer outcomes has not been extensively examined. Our objective was to determine the association between SES and event-free survival (EFS) among children with acute lymphoblastic leukemia (ALL) diagnosed in Ontario, Canada from 1995-2011 (N=1541) using Cox propo...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.leukres.2014.08.017
更新日期：2014-12-01 00:00:00
abstract：:DNAs from chronic granulocytic leukaemia (CGL) and chronic myelomonocytic leukaemia (CMML) were assayed for transforming genes by transfection into NIH 3T3 cells. Foci DNA was tagged with a geneticin-resistance cosmid, then followed through a drug selection and tumorigenicity assay. Activated Ha-ras genes, with point ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(88)90033-1
更新日期：1988-01-01 00:00:00
abstract：:The efficiency of gene transfer into human leukemia cell lines by electroporation was investigated. For both transient expression (beta-galactosidase gene) and stable transformation (neomycin resistance gene), the transfer efficiency into leukemia cell lines using a square wave pulse was superior to that using an expo...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90062-x
更新日期：1991-01-01 00:00:00
abstract：:Many patients with multiple myeloma (MM) are elderly (aged >65 years) or unfit, and therefore ineligible for stem-cell transplantation. The novel agents thalidomide, bortezomib, and lenalidomide have shown improved outcomes in these patients. This article discusses the role of continuous therapy in improving patient o...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/S0145-2126(12)70005-X
更新日期：2012-11-01 00:00:00
abstract：:Older patients with acute myelogenous leukemia (AML) have overexpression of P-glycoprotein (Pgp+), and this has been shown to correlate quantitatively with therapeutic outcome. Since Pgp-mediated efflux of cytotoxic drugs can be inhibited by the cyclosporine analogue, PSC 833, we investigated the use of this agent wit...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(00)00024-2
更新日期：2000-07-01 00:00:00
abstract：:The biological progression of chronic myeloid leukaemia is often associated with secondary cytogenetic abnormalities but the molecular mechanisms underlying this progression are poorly understood. This study explores the association of c-myc gene amplification with the progression of chronic myeloid leukaemia in fourt...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00097-6
更新日期：1998-10-01 00:00:00
abstract：:The goal of this study was to ascertain the specific effects of 17-N-Allylamino-17-demethoxygeldanamycin (17-AAG) treatment in human acute myelogenous leukemia (AML). Four human leukemia cell lines were treated with varying doses of 17-AAG followed by analysis of toxicity, apoptosis, proliferation, and cell cycle. Cel...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.05.024
更新日期：2010-11-01 00:00:00
abstract：:We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was promi...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.03.047
更新日期：2009-09-01 00:00:00
abstract：BACKGROUND:Myeloid sarcoma is a tumor mass that consists of myeloblasts or immature myeloid cells at an extramedullary site. Pathological diagnosis is very difficult based on morphology if systemic signs of disease are absent. The subtype of myeloid sarcoma is also minimally identifiable in the histological picture. F...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2016.01.002
更新日期：2016-03-01 00:00:00
abstract：:In-vitro infection of normal human lymphocytes with HTLV-I (human T-cell lymphotropic retrovirus type I) has been carried out to study the target cell specificity and the kinetics of infection. Cord blood (CBL) and adult peripheral blood lymphocytes (PBL) have been co-cultivated with irradiated HTLV-I donor cells (MT2...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90056-1
更新日期：1986-01-01 00:00:00
abstract：:Brazil is a wide country with huge contrasts. Its peculiarities can highlight environmental factors that could influence the frequencies of different cancers. The standard treatment and results achieved from several different areas of the country may not be found in others. The establishment of a national cooperative ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(01)00187-4
更新日期：2002-07-01 00:00:00
abstract：:The MTT cell viability assay is widely used in determining drug sensitivity profiles for patients with hematological malignancies and in primary screening of potential chemotherapeutic drugs. Because the multidrug resistance (MDR) phenotype is associated with these malignancies, and since many vital dyes are effluxed ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(92)90114-m
更新日期：1992-12-01 00:00:00
abstract：:The aim of the study was to investigate the expression of MIP-1 alpha and sclerostin in bone marrow of patients with multiple myeloma (MM), the possible association of the sclerostin and MIP-1 alpha with MBD and the clinical characteristics. 53 patients (29 M, 24 F), median age 64 years was studied. MIP-1 alpha, scler...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.02.010
更新日期：2014-05-01 00:00:00
abstract：:We evaluated the cytotoxicity of dimethylsphingosine (DMS) against four human leukemia cell lines: two acute (HL60 and a multi-drug resistance MDR-positive derivative HL60-dox) and two blast crisis chronic myelogenous leukemias (JFP1, from a treatment refractory patient and K562), and against blasts isolated from 11 l...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(01)00129-1
更新日期：2002-03-01 00:00:00
abstract：:We investigated Syk as a potential marker of CML progression. We observed a significant over-expression of Syk mRNA and constitutive phosphorylation of Syk Y348 in blast cells from six AP or BP-CML, but not in 15 CML in chronic phase. We could follow in vivo the recurrence of pSyk(348) throughout blast cell escape, de...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2014.11.014
更新日期：2015-03-01 00:00:00
abstract：:We described the clinical features and outcomes for 63 adult patients with acute myeloid leukemia (AML) with a translocation involving the 11q23 locus (MLL) who were treated at Memorial Sloan Kettering Cancer Center (MSK). The population included 40 female (63 %) and 23 male (37 %) patients, with a median age of 51 ye...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2020.106453
更新日期：2020-11-01 00:00:00
abstract：:We have investigated the production of metalloproteinases (MMP-1, MMP-2, MMP-9) and tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) in bone marrow stromal cells (BMSCs) of patients with multiple myeloma (MM) and a healthy control. The new findings of this paper is that BMSCs of the MM patients exhibited in...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.04.001
更新日期：2008-11-01 00:00:00
abstract：:To improve the recovery rate of high-risk patients with acute promyelocytic leukemia (APL), we used all-trans retinoic acid (ATRA)/arsenic trioxide (ATO)/daunorubicin combination in remission induction, daunorubicin and cytarabine in consolidation, and ATRA/ATO/methotrexate ± 6-mercaptopurine in maintenance treatment ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.03.027
更新日期：2012-07-01 00:00:00
abstract：:The ability of A-MuLV to transform bone marrow cells on in vitro culture in agarose is enhanced by inclusion of conditioned media during infection and culture. The conditioned medium of a non-virus producing A-MuLV transformed fibroblast cell line was synergistic with medium from Whitlock-Witte long-term bone marrow c...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90469-a
更新日期：1991-01-01 00:00:00
abstract：:Proliferative responses of mononuclear cells in liquid cultures to phytohemagglutinin, a T-cell mitogen, are depressed for a long time after allogeneic marrow transplantation. We examined the role of interleukin-2, a lymphokine important in T-cell mitogenesis, in the impaired phytohemagglutinin responses early after m...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90081-x
更新日期：1984-01-01 00:00:00
abstract：:C-kit, a tyrosine kinase receptor, is expressed on most myeloid blasts and is thought to be important in the pathogenesis of AML. Activation of the c-kit receptor leads to phosphorylation and activation of downstream signaling proteins, which are important for cell survival and proliferation. Here, we discuss the prog...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.08.019
更新日期：2008-06-01 00:00:00
abstract：:The amplified myeloma centrosome has been identified as a therapeutic target. The present study explored the expression and prognostic significance of the centrosome-associated protein PLK1 in myeloma and the effect of BI 2536, a potent and selective inhibitor of PLK1, on myeloma cells. High plasma cell expression of ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.07.016
更新日期：2011-12-01 00:00:00
abstract：:Essential thrombocythemia (ET) is an uncommon chronic myeloproliferative disorder with no cure. Patients with ET are at risk of different complications, and currently there are no optimal prognostic standards to predict severe post-diagnosis complications such as thrombosis and hemorrhage. In this study, we retrospect...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2018.03.013
更新日期：2018-06-01 00:00:00
abstract：:Acute myeloid leukemia (AML) is frequently treated with induction and consolidation chemotherapy. Consolidation chemotherapy can be delivered on an ambulatory basis, requiring some patients to travel long distances for treatment at specialized centers. We developed a shared care model where patients receive consolidat...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2017.05.023
更新日期：2017-08-01 00:00:00
abstract：:In a prospective study of 27 patients with acute myeloid leukaemia (AML) quality of life was studied using three different instruments, Life Ingredient Profile, Karnofsky Performance Scale and Vitagram. The course of the disease was followed during the induction period, remission, relapse and in the terminal phase. We...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)90028-0
更新日期：1994-04-01 00:00:00
abstract：:We recently confirmed that ciclopirox olamine inhibits Wnt/beta catenin signalling in myeloma. Griseofulvin (GF) has similar chemical features as compared to ciclopirox olamine. In this study the anti-tumor effect of GF was investigated. GF demonstrated a major apoptotic activity in various human and murine myeloma an...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.10.008
更新日期：2011-08-01 00:00:00
abstract：:Migration of eosinophil (Eo) into tissues is a hallmark of chronic eosinophilic leukaemia (CEL), but the exact mechanism involved in cell migration is unknown. We report on a patient with CEL who presented high expressions of VLA-4, LFA-1 and Mac-1 integrins on the Eo surface, increased chemotaxis of Eo to eotaxin, de...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.07.025
更新日期：2007-05-01 00:00:00
abstract：:The serum levels of interleukin 6 (IL6) are known to be elevated in two diseases of the elderly age, myelodysplastic syndrome (MDS) and multiple myeloma (MM). Authors suppose that one of the possible causes of this elevation could be a difference between these patients and healthy subjects in the frequency of polymorp...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.03.009
更新日期：2009-11-01 00:00:00
abstract：:The PRAME gene encodes an antigen recognized by autologous T lymphocytes and is expressed in trophoblasts, testis and frequently in human solid cancers and acute leukemias, making it a candidate for immunotherapy and for detecting MRD. We demonstrate expression of PRAME by RT-PCR in the peripheral blood or bone marrow...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(02)00217-5
更新日期：2003-05-01 00:00:00
