Abstract：
:The MTT cell viability assay is widely used in determining drug sensitivity profiles for patients with hematological malignancies and in primary screening of potential chemotherapeutic drugs. Because the multidrug resistance (MDR) phenotype is associated with these malignancies, and since many vital dyes are effluxed from MDR expressing cells, we have investigated whether the MDR phenotype interferes with the MTT assay. In CCRF-CEM and K562 human leukemic cell lines and drug-resistant sub-lines developed from them, comparison of the MTT assay with other cell viability assays showed significant variation in IC50 concentrations, although the resistance relative to the sensitive parent cell was correlated. Inclusion of verapamil, an inhibitor of drug efflux activity, had no effect on the MTT assay.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Marks DC,Belov L,Davey MW,Davey RA,Kidman ADdoi
10.1016/0145-2126(92)90114-mkeywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
1165-73issue
12eissn
0145-2126issn
1873-5835journal_volume
16pub_type
杂志文章abstract：:We hypothesized that the high early death rate (EDR) due to bleeding in acute promyelocytic leukemia (APL) is in part attributable to delays in all- trans retinoic acid (ATRA). We conducted a retrospective analysis of the timing of ATRA administration. 204 consecutive patients with newly diagnosed APL between 1992 and...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2013.05.007
更新日期：2013-09-01 00:00:00
abstract：:Chemotherapy resistance has been considered as a major problem for multiple myeloma (MM) treatment and bone marrow microenvironment plays a crucial role in the MM progression and chemoresistance. Recent studies reported that bone marrow mesenchymal stem cells derived from MM patients (MM-MSCs) revealed various charact...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2017.07.002
更新日期：2017-09-01 00:00:00
abstract：:We report on four cases of trisomy 14 as the sole anomaly. Three cases were myelodysplastic syndromes and one was a non-Hodgkin's lymphoma. This anomaly is mainly in myeloid disorders and still remains to be well documented. On the other hand, we show this anomaly to be also a non-random anomaly in lymphoproliferative...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(92)90181-6
更新日期：1992-01-01 00:00:00
abstract：:A retrospective cytogenetic study was performed to determine whether non-random chromosome aberrations were related to the outcome of marrow transplantation for advanced stage acute leukemia (AL) and chronic myelogenous leukemia (CML). The patients were registered on SWOG-8612, a randomized comparison of busulphan and...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/0145-2126(94)00162-4
更新日期：1995-06-01 00:00:00
abstract：:Allogeneic hematopoietic stem cell transplantation provides curative therapy for some patients with advanced hematologic malignancies. Disease response after allogeneic transplant is, at least in part, mediated by donor immune cells. In this report we describe a cellular therapy using haploidentical peripheral blood s...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2008.05.015
更新日期：2008-12-01 00:00:00
abstract：:Platelet transfusion refractoriness (PTR) is a major adverse event in the management of acute myeloid leukemia (AML). In a series of 897 adult patients with AML receiving intensive chemotherapy, we identified 41 patients (4.8%) with PTR. PTR was more frequently observed in parous women, patients with extra-medullary d...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2017.08.015
更新日期：2017-10-01 00:00:00
abstract：:Malignant B-1 cells derived from NZB mice, a murine model of chronic lymphocytic leukemia, produce significantly higher levels of IL-10 mRNA than normal B-1 or B cells. IL-10 may act as an autocrine growth factor for malignant B-1 cells. By addition of antisense oligodeoxynucleotides specific for IL-10 mRNA, we were a...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)00129-x
更新日期：1995-03-01 00:00:00
abstract：:We evaluated the molecular mechanism of telomerase activation by erythropoietin (EPO) in human erythroleukemic JAS-REN-A cells. Telomerase activity increased 3-4 fold after 3-24h of culture with EPO and was associated with increases in c-myc mRNA after 1-3h, of c-Myc protein after 3-6h, and of human telomerase reverse...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.11.002
更新日期：2011-03-01 00:00:00
abstract：:p23 is a heat shock protein 90 (Hsp90) cochaperone that plays a significant role in estrogen receptor (ER) alpha signal transduction and telomerase activity; it is up-regulated in several cancers. Recent studies have found that high level of p23 may promote tumor progression and poor prognosis in breast cancer patient...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.05.003
更新日期：2012-09-01 00:00:00
abstract：:Imatinib mesylate (IM) is the treatment of choice in patients with newly diagnosed chronic myeloid leukemia (CML), irrespectively of their age. Nevertheless, information regarding tolerability and responses in advanced-age patients, a subgroup in which co-morbidities and other factors may influence outcome, is scarce,...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.leukres.2011.01.017
更新日期：2011-09-01 00:00:00
abstract：:AML progenitor cells (AML-PC) undergo significant apoptosis in response to the deacetylase inhibitor (DACi) LAQ824 and lose the replating capacity which was not observed with the DACi valproic acid. Treatment of normal hematopoietic progenitor cells (HPC) with LAQ824 resulted in (i) inhibition of differentiation, (ii)...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.06.024
更新日期：2011-01-01 00:00:00
abstract：:Acute promyelocytic leukemia (APL) is highly malignant and frequently expresses the PML-RARalpha (promyelocytic leukemia-retinoic acid receptor-alpha) fusion protein. This fusion protein is targeted by all-trans retinoic acid (ATRA) and arsenic trioxide (As2O3), presently used in APL therapy. We have evaluated effects...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2007.06.021
更新日期：2008-02-01 00:00:00
abstract：:MONO-MAC-1 is a human cell line with properties of blood monocytes, which can be used as a model system to study monocytic functions in vitro. In the present study, we prepared a karyotype of MONO-MAC-1, analysed the growth behaviour, determined the presence of differentiation-associated antigens and studied the expre...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(96)00129-4
更新日期：1997-04-01 00:00:00
abstract：:To improve the recovery rate of high-risk patients with acute promyelocytic leukemia (APL), we used all-trans retinoic acid (ATRA)/arsenic trioxide (ATO)/daunorubicin combination in remission induction, daunorubicin and cytarabine in consolidation, and ATRA/ATO/methotrexate ± 6-mercaptopurine in maintenance treatment ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.03.027
更新日期：2012-07-01 00:00:00
abstract：:Leukemic cell apoptosis may be enhanced by appropriate oxidative stress. We report here the mechanism of Jurkat cell apoptosis by monochloramine (NH(2)Cl), a neutrophil-derived oxidant. NH(2)Cl induced caspase-dependent apoptosis, which was preceded by cytochrome c and Smac/Diablo release from mitochondria. Within 10m...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.07.009
更新日期：2009-01-01 00:00:00
abstract：:Zerumbone (ZER) is a potential anticancer natural compound, isolated from Zingiber zerumbet Smith. In this investigation, the anticancer properties of ZER were evaluated on cancer cells of T-acute lymphoblastic leukemia, CEM-ss. The results showed that ZER has cytotoxic effect against CEM-ss cells with an IC(50) of 8....
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2010.07.025
更新日期：2011-02-01 00:00:00
abstract：:Chronic myeloid leukemia (CML) is associated with overexpression of BCR-ABL1, a nonreceptor tyrosine kinase critical for malignant transformation. We investigated whether non-coding microRNAs (miRNAs) targeting BCR-ABL1 mRNA contribute to the pathogenesis of CML. Indeed, miR-30a targeted BCR-ABL1 and was underexpresse...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.12.003
更新日期：2013-03-01 00:00:00
abstract：:Little information is available about the risk factors and means to improve the survival rate of acute leukemia in a rare but often fatal complication after liver transplantation (LT). We report the development of AML-M2 in one of the 764 patients who underwent liver transplantation at our center, and review the liter...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.03.035
更新日期：2009-10-01 00:00:00
abstract：:A combination of monosomy 7 and translocation t(9;22) (q34;q11), rarely observed in acute lymphoblastic leukaemia (ALL), is here reported: a peculiarity of this case was that the "breakpoint cluster region" on chromosome 22 was not rearranged, as demonstrated by molecular analysis, and a new c-abl protein (p190) was f...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90114-7
更新日期：1987-01-01 00:00:00
abstract：:Five patients from the Kyushu area in Japan with adult T-cell leukaemia (ATL) refractory to conventional chemotherapeutic agents were treated with 5 mg/m2 of the adenosine deaminase inhibitor, 2'-deoxycoformycin (DCF), intravenously (i.v.) for 3 consecutive days, followed by 5 mg/m2 i.v. weekly. Two patients showed a ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(86)90251-1
更新日期：1986-01-01 00:00:00
abstract：:A number of inborn errors of purine metabolism have been associated with immunodeficiency diseases. From studies to the possible mechanism(s) leading to the defects in the immune system, it appeared that the accumulation of deoxyATP and deoxyGTP and the subsequent inhibition of ribonucleotide reductase played an impor...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/0145-2126(83)90042-5
更新日期：1983-01-01 00:00:00
abstract：:Inhibition of cyclooxygenase 2 (COX-2) by the selective COX-2 inhibitor celecoxib has been suggested as potentially useful for B-cell lymphoma therapy. However, additional pharmacological activities of celecoxib have been discovered and have challenged the notion that its antitumor effects are mediated primarily via t...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.09.028
更新日期：2010-02-01 00:00:00
abstract：:Based on Bcl-X(L) overexpression studies we identified type I and type II follicular lymphoma cell lines in response to TRAIL. We demonstrate here that either amount of caspase-8 activation or Bid cleavage could not define the dependence on mitochondria. Furthermore, an inhibitor of NF-kappaB, PDTC, enabled TRAIL to a...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.09.025
更新日期：2009-06-01 00:00:00
abstract：:The treatment of acute myeloid leukemia (AML) did not evolve profoundly in the last decades. Some improvement has been made for acute lymphoblastic leukemia (ALL). Emerging new treatment modalities, such as immunotherapy, are now beginning to be available for acute leukemia, mostly for patients suffering from ALL. Thi...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2017.06.011
更新日期：2017-09-01 00:00:00
abstract：:We describe the properties of three monoclonal antibodies (McAbs) (21H73, 37G7 and 49C12) against K562 cell surface antigens correlated with differentiation induced by 12-O-tetradecanoyl phorbol-13-acetate (TPA). Each of the McAbs immunoprecipitated K562 cell surface antigen with molecular weight (MW) of approximately...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90121-9
更新日期：1991-01-01 00:00:00
abstract：:Previous studies have shown that thymosin (fraction 5) is able to induce surface differentiation markers on normal murine bone marrow T-cell precursors. Phorbol ester (TPA) promotes differentiation of human leukaemic lymphoblasts as assessed by changes in phenotypic surface markers and terminal deoxynucleotidyl transf...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(83)90072-3
更新日期：1983-01-01 00:00:00
abstract：:Clofarabine (CLO), a purine nucleoside analog with promising efficacy in acute myeloid leukemia (AML), inhibits the ribonucleotidereductase, p53R2. We have shown that p53R2 mRNA is up-regulated by decitabine (DEC), another drug with promising activity in AML. We developed a pharmacodynamic model to characterize the in...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.07.015
更新日期：2012-11-01 00:00:00
abstract：:Promoter hypermethylation occurs early in leukemogenesis and seems to be associated with poor prognosis in acute lymphoblastic leukemia (ALL). The methylation status of the promoter region of six genes was analyzed in 71 children with ALL using methylation specific PCR (MSP). Calcitonin (CALCA) and E-cadherin (CDH1) w...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2005.11.016
更新日期：2006-07-01 00:00:00
abstract：:We designed a pharmacokinetic and pharmacodynamic phase I study of sequential topotecan (2.55-6.3mg/m2) by 72h infusion followed by five daily doses of etoposide for patients with refractory acute leukemia based upon synergistic anti-tumor activity of topoisomerase I and II inhibitors in vitro. Eight of the 29 patient...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/s0145-2126(02)00079-6
更新日期：2003-01-01 00:00:00
abstract：:In a patient affected with chronic lymphocytic leukemia with lymphocyte surface mu and kappa determinants and vacuolated bone marrow plasma cells, the serum contained polymers of a truncated mu chain and normal-sized kappa chains. These light chains were present as monomers and covalent dimers in studies performed und...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(93)90129-9
更新日期：1993-06-01 00:00:00
