Abstract：
:A class of designed self-assembling peptide nanofiber scaffolds with more than 99% water content has been shown to be a good biological material for cell culture. Here, we report the functionalization of one of these peptide scaffolds, RAD16-I (AcN-RADARADARADARADA-CONH2), by direct solid phase synthesis extension at the amino terminal with three short-sequence motifs. These motifs are present in two major protein components of the basement membrane, laminin 1 (YIGSR, RYVVLPR) and collagen IV (TAGSCLRKFSTM). These motifs have been previously shown to promote specific biological activities including endothelial cell adhesion, spreading, and tubular formation. Therefore, the generic functionalized peptide developed was AcN-X-GG-RADARADARADARADA-CONH2 with each motif represented by "X". We show in this work that these tailor-made peptide scaffolds enhance the formation of confluent cell monolayers of human aortic endothelial cells (HAEC) in culture. Moreover, additional assays designed to evaluate endothelial cell function showed that HAEC monolayers obtained on these scaffolds not only maintained LDL uptake activity but also enhanced nitric oxide release and elevated laminin 1 and collagen IV deposition. These results suggest that this new scaffold provide a better physiological substrate for endothelial cell culture and suggest its further application for biomedical research, cancer biology and regenerative biology.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Genové E,Shen C,Zhang S,Semino CEdoi
10.1016/j.biomaterials.2004.08.012keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
3341-51issue
16eissn
0142-9612issn
1878-5905pii
S0142-9612(04)00761-6journal_volume
26pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:The effect of hirudin, a known inhibitor of thrombin, was evaluated for whole blood samples in terms of platelet deposition/adhesion to a non-biological test surface (tetrafluoroethylene-propylene copolymer), adenosine diphosphate (ADP) release and reduction in platelet count during laminar shear flow for a shear rate...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(93)90009-q
更新日期：1993-09-01 00:00:00
abstract：:Calcium phosphate cements were first proposed as synthetic bone substitutes over two decades ago, however, they are characterised by slow chemical or cellular resorption and a slow osteointegration. In contrast, bone autograft has been shown to stimulate osteoclastogenesis and angiogenesis resulting in active bone rem...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.03.035
更新日期：2008-08-01 00:00:00
abstract：:A facile and efficient method to immobilize bioactive proteins onto polymeric substrate was established. Testis-specific protease 50 (TSP50) was immobilized on ultrafine biodegradable polymer fibers, i.e., (1) to prepare a propargyl-containing polymer P(LA90-co-MPC10) by introducing propargyl group into a cyclic carbo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.11.008
更新日期：2008-03-01 00:00:00
abstract：:In order to clarify the role of a primer on the adhesion between dental precious metals and resin, surface-enhanced Raman scattering (SERS) technique has been applied to the structural analysis of the adhesive monomer, 6-(N-(4-vinylbenzyl)propylamino)-1,3,5-triazine-2, 4-dithione (VBATDT) adsorbed on colloidal Au surf...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00238-5
更新日期：1999-05-01 00:00:00
abstract：:The peripheral nerve contains three barriers which include the blood-nerve barrier consisting of endoneurial vessels and the perineurium as well as autotypic junctions in Schwann cells. The perineurium prevents diffusion of perineurally injected drugs that can be used for selective regional pain control. It is compose...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.11.053
更新日期：2016-03-01 00:00:00
abstract：:Silver nanoparticles (AgNPs) have anti-cancer effect. However, whether and how these particles could inhibit the growth of acute myeloid leukemia (AML) cells is unclear. In the present study, we prepared AgNPs with various sizes and investigated their cytotoxic effect on AML cells. We found that AgNPs could inhibit th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.07.015
更新日期：2013-10-01 00:00:00
abstract：:Embryonic stem cells (ESCs) are promising cell sources for cell-based therapy. It has been established that the formation of ESC aggregates promotes their differentiation into the derivatives of all three germ layers. ESC aggregates are generally prepared via the formation of suspended spherical aggregates called embr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.05.003
更新日期：2009-09-01 00:00:00
abstract：:Construction of biodegradable, three-dimensional scaffolds for tissue engineering has been previously described using a variety of molding and rapid prototyping techniques. In this study, we report and compare two methods for fabricating poly(DL-lactide-co-glycolide) (PLGA) scaffolds with feature sizes of approximatel...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00052-8
更新日期：2003-06-01 00:00:00
abstract：:Apart from partial or total joint replacement, no surgical procedure is currently available to treat large and deep cartilage defects associated with advanced diseases such as osteoarthritis. In this work, we developed a perfusion bioreactor system to engineer human cartilage grafts in a size with clinical relevance f...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.08.009
更新日期：2010-12-01 00:00:00
abstract：:The aim of this study was to determine the biocompatibility and osteoconductive potential of a high-strength canasite glass ceramic. Glass-ceramic rods were produced using the lost-wax casting technique and implanted in the mid-shafts rabbit femurs. Implants were harvested at 4, 13 and 22 weeks and prepared for light ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00417-3
更新日期：2002-07-01 00:00:00
abstract：:The in vivo cellular microenvironment is regulated by a complex interplay of soluble factors and signaling molecules secreted by cells and it plays a critical role in the growth and development of normal and diseased tissues. In vitro systems that can recapitulate the microenvironment at the cellular level are needed ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.06.013
更新日期：2011-10-01 00:00:00
abstract：:Programmed cell death-ligand 1 (PD-L1)-based immune checkpoint blockade therapy using the anti-PD-L1 antibody is effective for a subset of patients with advanced metastatic melanoma but about half of the patients do not respond to the therapy because of the tumor immunosuppressive microenvironment. Immunogenic cell de...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120604
更新日期：2020-12-17 00:00:00
abstract：:The ability to restore heart function by replacement of diseased myocardium is one of the great challenges in biomaterials and regenerative medicine. Brown adipose derived stem cells (BADSCs) present a new source of cardiomyocytes to regenerate the myocardium after infarction. In this study, we explored an injectable ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.01.021
更新日期：2014-04-01 00:00:00
abstract：:Human induced pluripotent stem cell (hiPSC) derived angiogenesis models present a unique opportunity for patient-specific platforms to study the complex process of angiogenesis and the endothelial cell response to biomaterial and biophysical changes in a defined microenvironment. We present a refined method for differ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.11.032
更新日期：2019-02-01 00:00:00
abstract：:Two types of isoelastic endosseous dental implants were produced and their functionality was tested. One type consisted of a porous sintered TiTa30 alloy, the other had a special surface structure consisting of titanium wire loops. Their mechanical properties were optimized by the production parameter (sintering and d...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(93)90129-p
更新日期：1993-10-01 00:00:00
abstract：:The majority of epithelia in our organism perform barrier functions on being exposed to different fluids at the luminal and basal sides. To simulate this natural situation under in vitro conditions for biomaterial testing and tissue engineering the epithelia have to withstand mechanical and fluid stress over a prolong...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00186-7
更新日期：2002-02-01 00:00:00
abstract：:This work reports the development of new non-thrombogenic polymers based on the linear polymers of polyamidoamines (PAAs), having heparin binding ability, obtained by polyaddition of secondary amines to N,N'-methylene bis-acrylamide. PAAs could not be used directly in the making of blood-contacting materials due to th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00122-4
更新日期：2003-08-01 00:00:00
abstract：:Integrins represent the primary mechanism of cell-extracellular matrix interactions and control cell morphology, proliferation, and differentiation. We have previously shown that substrate-dependent modulation of adsorbed fibronectin (Fn) conformation alters alpha5beta1 integrin binding to Fn and directs C2C12 myoblas...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00387-8
更新日期：2002-06-01 00:00:00
abstract：:Bioglass 45S5 is a silica-based melt-derived glass, used in medical field as a bone regenerative material because of the deposition of a layer of hydroxy carbonate apatite (HCA) on the surface of the glass when immersed in body fluid. The present paper studies the early steps of reaction of 2-microm sized particles of...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.07.009
更新日期：2005-05-01 00:00:00
abstract：:Clinically used contrast agents for magnetic resonance imaging (MRI) suffer by the lack of specificity; short circulation time; and insufficient relaxivity. Here, a one-step combinatorial approach is described for the synthesis of magnetic lipid-polymer (hybrid) nanoparticles (MHNPs) encapsulating 5 nm ultra-small sup...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.07.003
更新日期：2013-10-01 00:00:00
abstract：:The histology and cytology of the tissue response to intra-articular insertion of particles of acrylic bone cement containing barium sulphate has been studied in the guinea pig knee joint. Following insertion of the acrylic, fibrinous debris admixed with particles became adherent to the synovial membrane. Cell prolife...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(84)90003-6
更新日期：1984-03-01 00:00:00
abstract：:Designing biomaterial scaffolds remains a major challenge in tissue engineering. Key to this challenge is improved understanding of the relationships between the scaffold properties and its degradation kinetics, as well as the cell interactions and the promotion of new matrix deposition. Here we present the use of non...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.12.049
更新日期：2008-05-01 00:00:00
abstract：:Electroconductive hydrogels (ECHs) are composite biomaterials that bring together the redox switching and electrical properties of inherently conductive electroactive polymers (CEPs) with the facile small molecule transport, high hydration levels and biocompatibility of cross-linked hydrogels. General methods for the ...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2009.12.052
更新日期：2010-04-01 00:00:00
abstract：:DNA vaccine is a milestone in contemporary vaccine development. It has considerably offset many shortcomings in conventional vaccines. Although DNA vaccines applied through 'traditional' high-pressure gene guns generally elicit high titers of protective immunity, such a practice however requires enormous investment in...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.07.029
更新日期：2009-10-01 00:00:00
abstract：:The purpose of this research was to develop porous poly(D,L-lactide-co-glycolide) (PLGA) scaffolds from which ascorbate-2-phosphate (AsAP) and dexamethasone (Dex) are continuously released for a month for osteogenesis of mesenchymal stem cells for bone tissue engineering. Porous PLGA matrices containing AsAP and Dex w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00358-2
更新日期：2003-11-01 00:00:00
abstract：:In spite of tremendous demand for the development and implementation of effective therapeutic strategies, limitations are still associated with doxorubicin-induced cardiotoxicity. Arjunolic acid (AA) has been shown to possess a multitude of biological functions. The purpose of the present study was to explore whether ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.03.048
更新日期：2011-07-01 00:00:00
abstract：:The optimal ratio of the polycation's amine to DNA phosphate group (N:P) for efficient polymer-based transfection always employs excess polycation versus DNA. Most of the excess polycation remains free in solution, unassociated with the polyplexes, but is essential for efficient transfection. The mechanism by which ex...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.03.010
更新日期：2011-07-01 00:00:00
abstract：:In vivo and in vitro degradation of high molecular weight poly(L-lactide) used for internal bone fixation has been investigated. Within 3 months as-polymerized, microporous PLLA (Mv = 6.8-9.5 X 10(5] exhibited a massive strength-loss (sigma b = 68-75 MPa to sigma b = 4 MPa) and decrease of Mv (90-95%). At week 39, the...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(87)90121-9
更新日期：1987-07-01 00:00:00
abstract：:Chitosan-silica xerogel hybrid membranes were fabricated using a sol-gel process and their potential applications in guided bone regeneration (GBR) were investigated in terms of their in vitro cellular activity and in vivo bone regeneration ability. TEM observation revealed that the silica xerogel was dispersed in the...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.10.025
更新日期：2009-02-01 00:00:00
abstract：:Human adipose-derived stem cells (hADSCs) were purified from a suspension of human adipose tissue cells (stromal vascular fraction) by the conventional culture method and by membrane filtration through polyurethane (PU) foam membranes. hADSCs can be obtained from a suspension of human adipose tissue cells using the me...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.08.027
更新日期：2012-11-01 00:00:00
