Enhanced expression of the osteoblastic phenotype on substrates that modulate fibronectin conformation and integrin receptor binding.


:Integrins represent the primary mechanism of cell-extracellular matrix interactions and control cell morphology, proliferation, and differentiation. We have previously shown that substrate-dependent modulation of adsorbed fibronectin (Fn) conformation alters alpha5beta1 integrin binding to Fn and directs C2C12 myoblast proliferation and differentiation (Mol. Biol. Cell 10 (1999) 785). The model substrates used in these experiments were bacteriological (untreated) polystyrene (B), tissue culture polystyrene (T), and type-I collagen-coated T (C). In the present study, we examined MC3T3-EI osteoblast-like cell differentiation on Fn-coated B, T, and C substrates. Immunofluorescence staining revealed substrate-dependent differences in integrin alpha5beta1 binding and clustering into focal adhesions (C > T > B), consistent with our previous integrin binding analysis. Alkaline phosphatase activity and matrix mineralization showed substrate-dependent differences (C > T > B, p < 0.05). Similar trends were observed for alkaline phosphatase, osteocalcin, and bone sialoprotein gene expression. Blocking experiments with antibodies directed against Fn completely inhibited matrix mineralization on Fn-coated C, indicating that Fn is critical to expression of the osteoblastic phenotype on this extracellular matrix component. These substrate-dependent differences in osteoblast differentiation correlated with differences in alpha5beta1 binding, suggesting that these differences arise from substrate modulation of integrin-matrix interactions. Substrate-dependent modulation of cell function may provide a versatile mechanism to control cell responses in numerous biomedical applications.






Stephansson SN,Byers BA,García AJ





Has Abstract


2002-06-01 00:00:00














  • A rapid-acting, long-acting insulin formulation based on a phospholipid complex loaded PHBHHx nanoparticles.

    abstract::The application of poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) for sustained and controlled delivery of hydrophilic insulin was made possible by preparing insulin phospholipid complex loaded biodegradable PHBHHx nanoparticles (INS-PLC-NPs). The INS-PLC-NPs produced by a solvent evaporation method showed a spher...


    pub_type: 杂志文章


    authors: Peng Q,Zhang ZR,Gong T,Chen GQ,Sun X

    更新日期:2012-02-01 00:00:00

  • Skeletal muscle tissue engineering: a maturation model promoting long-term survival of myotubes, structural development of the excitation-contraction coupling apparatus and neonatal myosin heavy chain expression.

    abstract::The use of defined in vitro systems to study the developmental and physiological characteristics of a variety of cell types is increasing, due in large part to their ease of integration with tissue engineering, regenerative medicine, and high-throughput screening applications. In this study, myotubes derived from feta...


    pub_type: 杂志文章


    authors: Das M,Rumsey JW,Bhargava N,Stancescu M,Hickman JJ

    更新日期:2009-10-01 00:00:00

  • Microgel encapsulated nanoparticles for glucose-responsive insulin delivery.

    abstract::An insulin delivery system that self-regulates blood glucose levels has the potential to limit hypoglycemic events and improve glycemic control. Glucose-responsive insulin delivery systems have been developed by coupling glucose oxidase with a stimuli-responsive biomaterial. However, the challenge of achieving desirab...


    pub_type: 杂志文章


    authors: Volpatti LR,Facklam AL,Cortinas AB,Lu YC,Matranga MA,MacIsaac C,Hill MC,Langer R,Anderson DG

    更新日期:2021-01-01 00:00:00

  • Simultaneous delivery of doxorubicin and gemcitabine to tumors in vivo using prototypic polymeric drug carriers.

    abstract::Copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) are prototypic and well-characterized polymeric drug carriers that have been broadly implemented in the delivery of anticancer therapeutics. To demonstrate that polymers, as liposomes, can be used for simultaneously delivering multiple chemotherapeutic agents to t...


    pub_type: 杂志文章


    authors: Lammers T,Subr V,Ulbrich K,Peschke P,Huber PE,Hennink WE,Storm G

    更新日期:2009-07-01 00:00:00

  • Multifunctional silica nanotubes for dual-modality gene delivery and MR imaging.

    abstract::This work demonstrated the development of multifunctional silica nanotubes (SNT) by functionalization of their inner void and outer surface with magnetic-fluorescence nanocomposites and cationic polymers, respectively. The successful construction of BPEI-SNT was established by electron energy loss spectroscopy in conj...


    pub_type: 杂志文章


    authors: Namgung R,Zhang Y,Fang QL,Singha K,Lee HJ,Kwon IK,Jeong YY,Park IK,Son SJ,Kim WJ

    更新日期:2011-04-01 00:00:00

  • Glioma therapy using tumor homing and penetrating peptide-functionalized PEG-PLA nanoparticles loaded with paclitaxel.

    abstract::By taking advantage of the excessively upregulated expression of neuropilin (NRP) on the surface of both glioma cells and endothelial cells of angiogenic blood vessels, the ligand of NRP with high affinity - tLyp-1 peptide, which also contains a CendR motif ((R/K)XX(R/K)), was functionalized to the surface of PEG-PLA ...


    pub_type: 杂志文章


    authors: Hu Q,Gao X,Gu G,Kang T,Tu Y,Liu Z,Song Q,Yao L,Pang Z,Jiang X,Chen H,Chen J

    更新日期:2013-07-01 00:00:00

  • Cancer stem cell labeling using poly(L-lysine)-modified iron oxide nanoparticles.

    abstract::Cell labeling using magnetic nanoparticles is an increasingly used approach in noninvasive behavior tracking, in vitro separation of cancer stem cells (CSCs), and CSC-based research in cancer therapy. However, the impact of magnetic labeling on the biological properties of targeted CSCs, such as self-renewal, prolifer...


    pub_type: 杂志文章


    authors: Wang X,Wei F,Liu A,Wang L,Wang JC,Ren L,Liu W,Tu Q,Li L,Wang J

    更新日期:2012-05-01 00:00:00

  • Neo-vascularization of the stroke cavity by implantation of human neural stem cells on VEGF-releasing PLGA microparticles.

    abstract::Replacing the tissue lost after a stroke potentially provides a new neural substrate to promote recovery. However, significant neurobiological and biotechnological challenges need to be overcome to make this possibility into a reality. Human neural stem cells (hNSCs) can differentiate into mature brain cells, but requ...


    pub_type: 杂志文章


    authors: Bible E,Qutachi O,Chau DY,Alexander MR,Shakesheff KM,Modo M

    更新日期:2012-10-01 00:00:00

  • Comparison of cell viability on anorganic bone matrix with or without P-15 cell binding peptide.

    abstract::Cell-extracellular cell matrix (ECM) interactions play a vital role in development including cytoskeletal structure, growth and differentiation. A 15 amino acid, non-RGD containing, peptide that is derived from type I collagen was tested for its ability to confer cell survival. Two routinely utilized bone graft materi...


    pub_type: 杂志文章


    authors: Hanks T,Atkinson BL

    更新日期:2004-08-01 00:00:00

  • Nanodiamonds coupled with plant bioactive metabolites: a nanotech approach for cancer therapy.

    abstract::Nanodiamond application in biotechnological and medical fields is nowadays in continuous progress. In fact, biocompatibility, reduced dimensions and high surface chemical interaction are specific features that make nanodiamonds perfect intracellular carriers of bioactive compounds. By confocal microscopy, we confirmed...


    pub_type: 杂志文章


    authors: Gismondi A,Reina G,Orlanducci S,Mizzoni F,Gay S,Terranova ML,Canini A

    更新日期:2015-01-01 00:00:00

  • Induction of protein oxidation by cobalt and chromium ions in human U937 macrophages.

    abstract::Metal particles and ions from hip prostheses have the potential to induce the production of reactive oxygen species (ROS), making them prime suspects for disturbing the cellular balance of oxidants/antioxidants (redox state of the cell). To better understand the cellular effect of metal ions from metal-on-metal prosth...


    pub_type: 杂志文章


    authors: Petit A,Mwale F,Tkaczyk C,Antoniou J,Zukor DJ,Huk OL

    更新日期:2005-07-01 00:00:00

  • Toward carbon nanotube-based imaging agents for the clinic.

    abstract::Among the many applications for carbon nanotubes (CNTs), their use in medicine has drawn special attention due to their potential for a variety of therapeutic and diagnostic applications. As progress toward clinical applications continues, monitoring CNTs in vivo will be essential to evaluate their biodistribution, po...


    pub_type: 杂志文章,评审


    authors: Hernández-Rivera M,Zaibaq NG,Wilson LJ

    更新日期:2016-09-01 00:00:00

  • Controllable synthesis of dual-MOFs nanostructures for pH-responsive artemisinin delivery, magnetic resonance and optical dual-model imaging-guided chemo/photothermal combinational cancer therapy.

    abstract::Theranostic nanoagents which integrate diagnostic and therapeutic moieties into a single platform have attracted broad attention in cancer therapy, however the development of more effective and less toxic diagnostic and therapeutic interventions is still of great urgency. Herein, novel core-shell PB@MIL-100(Fe) dual m...


    pub_type: 杂志文章


    authors: Wang D,Zhou J,Chen R,Shi R,Zhao G,Xia G,Li R,Liu Z,Tian J,Wang H,Guo Z,Wang H,Chen Q

    更新日期:2016-09-01 00:00:00

  • A review of the effects of the cell environment physicochemical nanoarchitecture on stem cell commitment.

    abstract::Physicochemical features of a cell nanoenvironment exert important influence on stem cell behavior and include the influence of matrix elasticity and topography on differentiation processes. The presence of growth factors such as TGF-β and BMPs on these matrices provides chemical cues and thus plays vital role in dire...


    pub_type: 杂志文章,评审


    authors: Das RK,Zouani OF

    更新日期:2014-07-01 00:00:00

  • Long-term clinical outcomes following the use of synthetic hydroxyapatite and bone graft in impaction in revision hip arthroplasty.

    abstract::Impaction grafting using morsellised allograft bone restores bone stock, but carries the potential for transmission of infection. Synthetic bone graft substitutes can eliminate this risk but may, however, influence outcome. In this study we tested the hypothesis that a 50/50 mix of hydroxyapatite and allograft does no...


    pub_type: 临床试验,杂志文章


    authors: Aulakh TS,Jayasekera N,Kuiper JH,Richardson JB

    更新日期:2009-03-01 00:00:00

  • A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces.

    abstract::The long-term patency rates of vascular grafts and stents are limited by the lack of surface endothelialisation of the implanted materials. We have previously reported that GRGDS and WQPPRARI peptide micropatterns increase the endothelialisation of prosthetic materials in vitro. To investigate the mechanisms by which ...


    pub_type: 杂志文章


    authors: Hoesli CA,Garnier A,Juneau PM,Chevallier P,Duchesne C,Laroche G

    更新日期:2014-01-01 00:00:00

  • Silicon substitution in the calcium phosphate bioceramics.

    abstract::Silicon (Si) substitution in the crystal structures of calcium phosphate (CaP) ceramics such as hydroxyapatite (HA) and tricalcium phosphate (TCP) generates materials with superior biological performance to stoichiometric counterparts. Si, an essential trace element required for healthy bone and connective tissues, in...


    pub_type: 杂志文章,评审


    authors: Pietak AM,Reid JW,Stott MJ,Sayer M

    更新日期:2007-10-01 00:00:00

  • Modeling the controllable pH-responsive swelling and pore size of networked alginate based biomaterials.

    abstract::Semisynthetic network alginate polymer (SNAP), synthesized by acetalization of linear alginate with di-aldehyde, is a pH-responsive tetrafunctionally linked 3D gel network, and has potential application in oral delivery of protein therapeutics and active biologicals, and as tissue bioscaffold for regenerative medicine...


    pub_type: 杂志文章


    authors: Chan AW,Neufeld RJ

    更新日期:2009-10-01 00:00:00

  • Macrophage response to cross-linked and conventional UHMWPE.

    abstract::To prevent wear debris-induced osteolysis and aseptic loosening, cross-linked ultra-high molecular weight polyethylene's (UHMWPE) with improved wear resistance have been developed. Hip simulator studies have demonstrated very low wear rates with these new materials leading to their widespread clinical use. However, th...


    pub_type: 杂志文章


    authors: Sethi RK,Neavyn MJ,Rubash HE,Shanbhag AS

    更新日期:2003-07-01 00:00:00

  • Histological and biochemical evaluation of osteogenic response in porous hydroxyapatite coated alumina ceramics.

    abstract::We compared the osteogenic response in porous alumni (Al) and hydroxyapatite coated porous alumni ceramic (HA/Al) by grafting rat bone marrow cells with these implants subcutaneously in the back of syngeneic rats. The ceramics did not show any bone formation without marrow, but did when combined with marrow cells. The...


    pub_type: 杂志文章


    authors: Takaoka T,Okumura M,Ohgushi H,Inoue K,Takakura Y,Tamai S

    更新日期:1996-08-01 00:00:00

  • Differential alkaline phosphatase responses of rat and human bone marrow derived mesenchymal stem cells to 45S5 bioactive glass.

    abstract::Bioactive glass is used as both a bone filler and as a coating on implants, and has been advocated as a potential osteogenic scaffold for tissue engineering. Rat-derived mesenchymal stem cells (MSCs) show elevated levels of alkaline phosphatase activity when grown on 45S5 bioactive glass as compared to standard tissue...


    pub_type: 杂志文章


    authors: Reilly GC,Radin S,Chen AT,Ducheyne P

    更新日期:2007-10-01 00:00:00

  • Phenformin-loaded polymeric micelles for targeting both cancer cells and cancer stem cells in vitro and in vivo.

    abstract::Conventional cancer chemotherapy often fails as most anti-cancer drugs are not effective against drug-resistant cancer stem cells. These surviving cancer stem cells lead to relapse and metastasis. In this study, an anti-diabetic drug, phenformin, capable of eliminating cancer stem cells was loaded into micelles via se...


    pub_type: 杂志文章


    authors: Krishnamurthy S,Ng VW,Gao S,Tan MH,Yang YY

    更新日期:2014-11-01 00:00:00

  • Scanning electron microscopy of the metal-tissue interface. I. Fixation methods and interpretation of results.

    abstract::Scanning electron microscopy has been used to study the interface between implants of pure metals and the muscular tissue of rats. Several problems arise in the interpretation of the observations made, due to the potential for disruption to the tissue and the exacerbation of any corrosion that may have occurred in viv...


    pub_type: 杂志文章


    authors: McNamara A,Williams DF

    更新日期:1982-07-01 00:00:00

  • Potential hepatoprotective effects of fullerenol C60(OH)24 in doxorubicin-induced hepatotoxicity in rats with mammary carcinomas.

    abstract::The aim of this study was to investigate the potential protective role of fullerenol C60(OH)24 on doxorubicin-induced liver toxicity using in vivo (female Sprague-Dawley rats) and in vitro (human hepatocellular carcinoma - HepG2; colorectal adenocarcinoma cell lines - Caco-2) approaches. The first (healthy control) an...


    pub_type: 杂志文章


    authors: Injac R,Perse M,Obermajer N,Djordjevic-Milic V,Prijatelj M,Djordjevic A,Cerar A,Strukelj B

    更新日期:2008-08-01 00:00:00

  • Synthesis, bioactivity and specificity of glucagon-like peptide-1 (7-37)/polymer conjugate to isolated rat islets.

    abstract::In order to increase the functionality of islets encapsulated in a biohybrid artificial pancreas (BAP), it was proposed that co-encapsulation with insulinotropic agents would improve insulin secretion from islets. To prevent agents from leaking out, conjugation with high-molecular-weight polymers was inevitable. In th...


    pub_type: 杂志文章


    authors: Kim S,Wan Kim S,Bae YH

    更新日期:2005-06-01 00:00:00

  • Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair.

    abstract::Chitosan (CHT)/poly(ɛ-caprolactone) (PCL) blend 3D fiber-mesh scaffolds were studied as possible support structures for articular cartilage tissue (ACT) repair. Micro-fibers were obtained by wet-spinning of three different polymeric solutions: 100:0 (100CHT), 75:25 (75CHT) and 50:50 (50CHT) wt.% CHT/PCL, using a commo...


    pub_type: 杂志文章


    authors: Neves SC,Moreira Teixeira LS,Moroni L,Reis RL,Van Blitterswijk CA,Alves NM,Karperien M,Mano JF

    更新日期:2011-02-01 00:00:00

  • The influence of BMP-2 and its mode of delivery on the osteoconductivity of implant surfaces during the early phase of osseointegration.

    abstract::Osteogenic agents, such as bone morphogenetic protein-2 (BMP-2), can stimulate the degradation as well as the formation of bone. Hence, they could impair the osteoconductivity of functionalized implant surfaces. We assessed the effects of BMP-2 and its mode of delivery on the osteoconductivity of dental implants with ...


    pub_type: 杂志文章


    authors: Liu Y,Enggist L,Kuffer AF,Buser D,Hunziker EB

    更新日期:2007-06-01 00:00:00

  • Anterior cruciate ligament regeneration using braided biodegradable scaffolds: in vitro optimization studies.

    abstract::The anterior cruciate ligament (ACL) is the most commonly injured intra-articular ligament of the knee, and limitations in existing reconstruction grafts have prompted an interest in tissue engineered solutions. Previously, we reported on a tissue-engineered ACL scaffold fabricated using a novel, three-dimensional bra...


    pub_type: 杂志文章


    authors: Lu HH,Cooper JA Jr,Manuel S,Freeman JW,Attawia MA,Ko FK,Laurencin CT

    更新日期:2005-08-01 00:00:00

  • Synthesis and characterization of PVNO and PVNO-PVP hydrogels.

    abstract::Hydrogels of crosslinked poly(2-vinylpyridine-1-oxide), PVNO, and poly(2-vinylpyridine-1-oxide)-poly(N-vinyl-pyrrolidone), PNVO-PVP, were synthesized. PVNO hydrogels were found to be degradable in a pseudo-biological medium and their stability could be increased by increasing the relative concentration of the crosslin...


    pub_type: 杂志文章


    authors: Hasirci VN

    更新日期:1981-01-01 00:00:00

  • Effects of topography and composition of titanium surface oxides on osteoblast responses.

    abstract::To investigate the roles of composition and characteristics of titanium surface oxides in cellular behaviour of osteoblasts, the surface oxides of titanium were modified in composition and topography by anodic oxidation in two kinds of electrolytes, (a) 0.2 M H(3)PO(4), and (b) 0.03 M calcium glycerophosphate (Ca-GP) ...


    pub_type: 杂志文章


    authors: Zhu X,Chen J,Scheideler L,Reichl R,Geis-Gerstorfer J

    更新日期:2004-08-01 00:00:00