Abstract：
:Embryonic stem cells (ESCs) are promising cell sources for cell-based therapy. It has been established that the formation of ESC aggregates promotes their differentiation into the derivatives of all three germ layers. ESC aggregates are generally prepared via the formation of suspended spherical aggregates called embryoid bodies (EBs). Because the differentiation efficiency depends on the size of EBs, it becomes one of the research topics how to prepare size-controlled EBs in a scalable manner for reproducible and high-throughput experiments. Here, we have developed a novel culture method that enables simple mass preparation of size-controlled ESC aggregates on a culture surface instead of floating EBs. We developed a maskless photolithography device that enabled rapid fabrication of micropatterned surfaces. Utilizing this device, we fabricated the culture substrates the surfaces of which comprised arrays of cell-adhesive circular micro-domains (100-400 microm in diameter) and the rest of non-cell-adhesive domains. We seeded mouse ESCs on this substrate and prepared size-controlled ESC aggregates on the micro-domains. We analyzed cardiac differentiation in the ESC aggregates and found that the optimal diameter of micro-domains was 200 microm. The present method is useful for the simple and reproducible mass preparation of ESC-derived differentiated cells and high-throughput assays.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Sasaki D,Shimizu T,Masuda S,Kobayashi J,Itoga K,Tsuda Y,Yamashita JK,Yamato M,Okano Tdoi
10.1016/j.biomaterials.2009.05.003subject
Has Abstractpub_date
2009-09-01 00:00:00pages
4384-9issue
26eissn
0142-9612issn
1878-5905pii
S0142-9612(09)00478-5journal_volume
30pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:A biocompatible hydrogel of poly[N-(2-hydroxypropyl)methacrylamide] (PHPMA) which includes the cell-adhesive region of fibronectin Arg-Gly-Asp was synthesized and its structure, rheological and dielectric properties were characterized. The ability of a PHPMA-RGD hydrogel to promote tissue regeneration and support axon...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00354-9
更新日期：2001-05-01 00:00:00
abstract：:Substitution with carboxylic and benzylamine sulphonated groups conferred on dextran both antithrombic activity and the capacity to inhibit formation of the amplification C3 convertase of complement. In dextrans substituted with carboxylic groups (greater than 40%), a high content of sulphonate (greater than 10%) resu...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(85)90040-7
更新日期：1985-01-01 00:00:00
abstract：:The objective of the study was to examine the effect of alkali ion release, pH control and buffer capacity on the expression of the osteoblastic phenotype. In addition we determined the importance of modifications of the surface of porous bioactive glass (BG) on the activity of rat calvaria osteoblasts in vitro. We fo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(96)00059-2
更新日期：1997-02-01 00:00:00
abstract：:Fracture toughness and hardness properties of CaO-P2O5 glass-reinforced hydroxyapatite composites have been assessed using indentation techniques and results calculated according to Laugier and Evans' equations. Both properties showed to be dependent upon several microstructural characteristics, namely residual porosi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00112-x
更新日期：1999-11-01 00:00:00
abstract：:This article reports a novel preparation method of poly(ethylene glycol) (PEG)-polystyrene (PST) amphiphilic block copolymers with well-defined block lengths by using photopolymerization of an iniferter, benzyl N,N-diethyldithiocarbamate. PEG macroiniferters, which were prepared by end-capping of PEG monomethyl ethers...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00252-x
更新日期：1999-05-01 00:00:00
abstract：:The aim of this study, in rabbit tibia, was an evaluation of the early reactions of the tissues to the insertion of polylactic membranes, used in connection with titanium implants. The specimens were retrieved after 1-4 weeks, and a histological analysis was performed. It was possible to see that, in the early implant...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00173-7
更新日期：1998-05-01 00:00:00
abstract：:Modeling the interaction between the supportive stroma and the hematopoietic stem and progenitor cells (HSPC) is of high interest in the regeneration of the bone marrow niche in blood disorders. In this work, we present an injectable co-culture system to study this interaction in a coherent in vitro culture and in viv...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.119665
更新日期：2020-02-01 00:00:00
abstract：:A comparative study of in vitro bioactivity of a sol-gel glass with composition 55% SiO2-41%CaO-4%P2O5 has been carried out by soaking it in a simulated body fluid (SBF) with and without exchange of this solution. When SBF is kept constant (static assay) the solution composition changes, increasing the Ca2+ concentrat...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00419-1
更新日期：2001-08-01 00:00:00
abstract：:Collagen, type I, is a highly abundant natural protein material which has been cross-linked by a variety of methods including chemical agents, physical heating and UV irradiation with the aim of enhancing its physical characteristics such as mechanical strength, thermal stability, resistance to proteolytic breakdown, ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.04.017
更新日期：2005-11-01 00:00:00
abstract：:The use of non-viral nanoparticulate systems for the delivery of therapeutic agents is receiving considerable attention for medical and pharmaceutical applications. This increasing interest results from the fact that these systems can be designed to meet specific physicochemical requirements, and they display low toxi...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2005.05.044
更新日期：2005-12-01 00:00:00
abstract：:Based on the hypothesis that bone cements cause changes in the production of transforming growth factor-beta 1 (TGF-beta1) by bone cells, the effects of four acrylic bone cements (Sulfix-60, CMW 1, CMW 2 and CMW 3) were examined using the osteoblast-like cell line MG63. The extracts in MEM of the cements were tested, ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00109-0
更新日期：2002-01-01 00:00:00
abstract：:Gelatin and dextran were blended and crosslinked to form enzymatically degradable interpenetrating polymeric networks (IPNs) as materials for degradable implants. Several crosslinking methods were investigated including treatment with glyceraldehyde, thermal hardening, and chelation of dextran by divalent metal cation...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00057-0
更新日期：2000-10-01 00:00:00
abstract：:The regeneration of large bone defects remains an unsolved clinical problem, which could benefit from recent findings on the biology of skeletal stem and progenitor cells. The elucidation of conditions to specifically control their dynamic and function will likely enable the development of novel treatment strategies. ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.119278
更新日期：2019-10-01 00:00:00
abstract：:This article introduces novel biocompatible tissue adhesives that do not involve any chemical or biochemical reactions, during their application in vivo. The use of these new adhesives is based exclusively on their temperature-dependent rheological properties. Since biocompatibility and biodegradability are additional...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.01.040
更新日期：2004-12-01 00:00:00
abstract：:Heparin-conjugated electrospun poly(ε-caprolactone) (PCL)/gelatin scaffolds were developed to provide controlled release of platelet-derived growth factor-BB (PDGF-BB) and allow prolonged bioactivity of this molecule. A mixture of PCL and gelatin was electrospun into three different morphologies. Next, heparin molecul...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.06.017
更新日期：2012-10-01 00:00:00
abstract：:Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion. However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials. Here we present the success...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.05.003
更新日期：2014-08-01 00:00:00
abstract：:A novel biodegradable polymer blend was developed for potential biomedical applications. A 50:50 poly(lactide-co-glycolide) (PLAGA) was blended in a 50:50 ratio with the followiing polyphosphazenes (PPHOS): poly[(25% ethyl glycinato)(75% p-methylphenoxy)phosphazene[, poly[(50% ethyl glycinato)(50% p-methylphenoxy)phos...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)80009-9
更新日期：1997-12-01 00:00:00
abstract：:Membranes, clinically used to improve bone regeneration according to the osteopromotion principle, have primarily been made of expanded polytetrafluoroethylene (Gore-Tex Membrane). Recently, different types of biodegradable membranes have become available. This investigation explored the osteopromotive potential of 10...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(95)93857-a
更新日期：1995-05-01 00:00:00
abstract：:The electrospinning process was utilized successfully to fabricate the random oriented and aligned electrically conductive nanofibers of biodegradable poly-DL-lactide (PLA) in which multiwalled carbon nanotubes (MWCNTs) were embedded. The topographical features of the composite nanofibers were characterized by SEM. Th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.01.051
更新日期：2011-04-01 00:00:00
abstract：:Bioactive glasses are potentially useful as bone defect fillers, and vascular endothelial growth factor (VEGF) has demonstrated benefit in bone regeneration as well. We hypothesized that the specific combination of prolonged localized VEGF presentation from a matrix coated with a bioactive glass may enhance bone regen...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.01.033
更新日期：2006-06-01 00:00:00
abstract：:The aim of this in vitro study was to investigate the effect of low-level laser therapy (LLLT) on the attachment, proliferation, differentiation and production of transforming growth factor-ss(1) (TGF-beta(1)) by human osteoblast-like cells (HOB). Cells derived from human mandibular bone were exposed to GaAlAs diode l...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.09.033
更新日期：2005-06-01 00:00:00
abstract：:Poly(ether-ether-ketone) (PEEK) has been used as a load bearing orthopaedic implant material with clinical success. All of the orthopaedic applications contain stress concentrations (notches) in their design; however, little work has been done to examine the fatigue behavior of PEEK in the presence of a notch. This wo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.08.032
更新日期：2010-12-01 00:00:00
abstract：:Disruption of peripheral nerves due to trauma is a frequently occurring clinical problem. Gaps in the nerve are bridged by guiding the regenerating nerves along autologous grafts or artificial guides. This review gives an overview on the different methods of nerve repair techniques. Conventional suturing techniques ar...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/s0142-9612(03)00504-0
更新日期：2004-04-01 00:00:00
abstract：:Covalent antithrombin-heparin complex (ATH) was covalently grafted to a polycarbonate urethane (Corethane) endoluminal graft (a kind gift of Corvita Corporation) after being activated using 0.3% m/m NaOCl in 0.15 M phosphate pH 6.0. ATH graft density (1.98 x 10(-7) mol/m2) was 6 times the maximum amount of unfractiona...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00135-1
更新日期：2002-01-01 00:00:00
abstract：:Delivery of polynucleotide-based therapeutics into target cells involves interactions with glycosaminoglycan chains that are located on cell membrane milieu. Mechanisms governing glycosaminoglycan-mediated changes in the nanoparticulate structures of polymer-polynucleotide complexes are unknown, and cannot be fully el...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.11.037
更新日期：2018-02-01 00:00:00
abstract：:Bone marrow is the primary hematopoietic organ, which is involved in multiple malignant diseases including acute and chronic leukemia, multiple myeloma, myelodysplastic syndromes, and bone metastases from solid tumors. These malignancies affect normal homeostasis and reshape the bone marrow microenvironment. There are...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2017.11.029
更新日期：2018-02-01 00:00:00
abstract：:Materials used for total knee arthroplasty (TKA), may elicit an immune response whose role in the outcome of the arthroplasty is still unclear. The aim of this study was to evaluate the frequency of sensitization in patients who had undergone TKA, and the clinical impact of this event on the outcome of the implant. Ni...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.11.038
更新日期：2008-04-01 00:00:00
abstract：:Hepatocytes are anchorage-dependent cells sensitive to microenvironment; the control of the physicochemical properties of the extra-cellular matrices may be useful to the maintenance of hepatocyte functions in vitro for various applications. In a microcapsule-based 3-D hepatocyte culture microenvironment, we could con...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.08.017
更新日期：2005-06-01 00:00:00
abstract：:Block copolymer prodrugs (BCPs) have attracted considerable attentions in clinical translation of nanomedicine owing to their self-assembly into well-defined core-shell nanoparticles for improved pharmacokinetics, stability in blood circulation without drug leakage, and optimized biodistribution. However, a cascade of...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.11.006
更新日期：2018-02-01 00:00:00
abstract：:Bioengineered tissue equivalents should provide physiologically relevant biochemical and mechanical cues to support the growth and differentiation of seeded cells. Herein, tubular dense collagen constructs (TDCCs) with collagen content comparable to native extracellular matrix were used to investigate the effect of sh...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.11.025
更新日期：2013-03-01 00:00:00
