Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices.

abstract：:The development of biohybrid organs (BHO) will benefit from improved membranes regarding transport and cell contacting properties. Here we describe in a first study the development and testing of membranes made of polyacrylonitrile (PAN) and polysulfone (PSU) for the immobilisation of kidney epithelial cells. Comparat...

journal_title：Biomaterials

authors： Fey-Lamprecht F,Groth T,Albrecht W,Paul D,Gross U

更新日期：2000-01-01 00:00:00

abstract：:Tissue engineering therapies require biomaterials capable of encouraging an angiogenic response. To dissect the influence of different pro-angiogenic stimuli a set of starPEG-heparin hydrogels with varied physicochemical properties was used as a highly efficient reservoir and tunable delivery system for basic fibrobla...

journal_title：Biomaterials

authors： Zieris A,Prokoph S,Levental KR,Welzel PB,Grimmer M,Freudenberg U,Werner C

更新日期：2010-11-01 00:00:00

abstract：:Heparin-conjugated electrospun poly(ε-caprolactone) (PCL)/gelatin scaffolds were developed to provide controlled release of platelet-derived growth factor-BB (PDGF-BB) and allow prolonged bioactivity of this molecule. A mixture of PCL and gelatin was electrospun into three different morphologies. Next, heparin molecul...

journal_title：Biomaterials

authors： Lee J,Yoo JJ,Atala A,Lee SJ

更新日期：2012-10-01 00:00:00

abstract：:In this in vitro study, the biocompatibility of acrylamide-based hydrogels such as acrylamide/crotonic and acrylamide/itaconic acid, prepared by using gamma-rays, has been investigated against some biochemical parameters of human serum. The swelling kinetics of acrylamide/crotonic and acrylamide/itaconic acid hydrogel...

journal_title：Biomaterials

authors： Karadağ E,Saraydin D,Cetinkaya S,Güven O

更新日期：1996-01-01 00:00:00

abstract：:Although synergistic therapy for tumors has displayed significant promise for effective treatment of cancer, developing a simple and effective strategy to build a multi-functional nanoplatform is still a huge challenge. By virtue of the characteristics of tumor microenvironment, such as hypoxia, slight acidity and H2O...

journal_title：Biomaterials

authors： Wang M,Chang M,Chen Q,Wang D,Li C,Hou Z,Lin J,Jin D,Xing B

更新日期：2020-09-01 00:00:00

abstract：:Adipose-derived stem cells (ASCs) have valuable applications in regenerative medicine, but maintaining the stemness of ASCs during in vitro culture is still a challenging issue. In this study, human ASCs spontaneously formed three-dimensional spheroids on chitosan films. Most ASCs within the spheroid were viable, and ...

journal_title：Biomaterials

authors： Cheng NC,Wang S,Young TH

更新日期：2012-02-01 00:00:00

abstract：:The magnetic resonance (MR) functionalities of pullulan-conjugated gadolinium diethylene triamine pentaacetate (Gd-DTPA-Pullulan) as a new hepatocyte-specific contrast agent were evaluated. Pullulan, which specifically accumulates on hepatocytes via asialoglycoprotein receptors, was chemically linked with Gd-DTPA. Gd-...

journal_title：Biomaterials

authors： Yim H,Yang SG,Jeon YS,Park IS,Kim M,Lee DH,Bae YH,Na K

更新日期：2011-08-01 00:00:00

abstract：:A class of designed self-assembling peptide nanofiber scaffolds with more than 99% water content has been shown to be a good biological material for cell culture. Here, we report the functionalization of one of these peptide scaffolds, RAD16-I (AcN-RADARADARADARADA-CONH2), by direct solid phase synthesis extension at ...

journal_title：Biomaterials

authors： Genové E,Shen C,Zhang S,Semino CE

更新日期：2005-06-01 00:00:00

abstract：:Morphological changes of human polymorphonuclear neutrophils (PMN) adhering to hydrophilic (glass) and hydrophobic (FEP-Teflon, polyethylene, polypropylene) surfaces were studied in a parallel-plate flow chamber at the light and scanning electron microscopical levels. The PMN were exposed to a shear stress of 0.19 Pa ...

journal_title：Biomaterials

authors： Tomczok J,Sliwa-Tomczok W,Klein CL,van Kooten TG,Kirkpatrick CJ

更新日期：1996-07-01 00:00:00

abstract：:Photodynamic therapy (PDT) is a new therapeutic approach for the palliative treatment of malignant bile duct obstruction. In this study, we designed photosensitizer-embedded self-expanding nonvascular metal stent (PDT-stent) which allows repeatable photodynamic treatment of cholangiocarcinoma without systemic injectio...

journal_title：Biomaterials

authors： Bae BC,Yang SG,Jeong S,Lee DH,Na K,Kim JM,Costamagna G,Kozarek RA,Isayama H,Deviere J,Seo DW,Nageshwar Reddy D

更新日期：2014-10-01 00:00:00

abstract：:Physicochemical features of a cell nanoenvironment exert important influence on stem cell behavior and include the influence of matrix elasticity and topography on differentiation processes. The presence of growth factors such as TGF-β and BMPs on these matrices provides chemical cues and thus plays vital role in dire...

journal_title：Biomaterials

authors： Das RK,Zouani OF

更新日期：2014-07-01 00:00:00

abstract：:The effect of inclusion of an immobilization matrix and the capsule membrane permeability on the viability, metabolic activity, and proliferation of encapsulated HEK cells was investigated in vitro. In the absence of a matrix, a particular transfected HEK cell line formed a single aggregate in the core of the poly(hyd...

journal_title：Biomaterials

authors： Lahooti S,Sefton MV

更新日期：2000-05-01 00:00:00

abstract：:Keratins are a family of structural proteins that can be isolated from a variety of tissues. "Soft" keratins are cytoskeletal elements found in epithelial tissues while protective tissues such as nails, hooves, and hair are composed of "hard" keratins. Hard keratins have been the subject of biomaterials investigations...

journal_title：Biomaterials

authors： Hill P,Brantley H,Van Dyke M

更新日期：2010-02-01 00:00:00

abstract：:An artificial receptor for creatinine was synthesized by the method of molecularly imprinted polymer (MIP). beta-Cyclodextrin was used as a monomer cross-linked with epichlorohydrin in the presence of creatinine, which was a template for the imprinting. Different molar ratios of monomer to template were used to synthe...

journal_title：Biomaterials

authors： Tsai HA,Syu MJ

更新日期：2005-05-01 00:00:00

abstract：:Hydroxy-fluorapatites (OH-FAps) occur biologically in teeth and form the basis for application as biomaterials. This work aims to synthesize a series of fluoride substituted calcium hydroxyapatites (OHAps) to determine how fluoride influences the synthesis and the resulting characteristics of solid solutions. OH-FAPs ...

journal_title：Biomaterials

authors： Rodríguez-Lorenzo LM,Hart JN,Gross KA

更新日期：2003-09-01 00:00:00

abstract：:The engineering of functional skeletal muscle tissue substitutes holds promise for the treatment of various muscular diseases and injuries. However, no tissue fabrication technology currently exists for the generation of a relatively large and thick bioartificial muscle made of densely packed, uniformly aligned, and d...

journal_title：Biomaterials

authors： Bian W,Bursac N

更新日期：2009-03-01 00:00:00

abstract：:Hydrogels are commonly used as artificial extracellular matrices for 3D cell culture and for tissue engineering. Viscoelastic hydrogels with tunable stress relaxation have recently been developed, and stress relaxation in the hydrogels has been found to play a key role in regulating cell behaviors such as differentiat...

journal_title：Biomaterials

authors： Nam S,Stowers R,Lou J,Xia Y,Chaudhuri O

更新日期：2019-04-01 00:00:00

abstract：:Local signals from tissue-specific extracellular matrix (ECM) microenvironments, including matrix adhesive ligand, mechanical elasticity and micro-scale geometry, are known to instruct a variety of stem cell differentiation processes. Likewise, these signals converge to provide multifaceted, mechanochemical cues for h...

journal_title：Biomaterials

authors： Floren M,Tan W

更新日期：2015-08-01 00:00:00

abstract：:Docetaxel (DOC) produces anti-tumor effects by inducing apoptosis and inhibiting cell growth. However, its clinical application is limited by its hydrophobicity and low biocompatibility. Therefore, improving DOC's water solubility, biocompatibility, and anti-tumor effects are important goals that will improve its clin...

journal_title：Biomaterials

authors： Gao X,Wang S,Wang B,Deng S,Liu X,Zhang X,Luo L,Fan R,Xiang M,You C,Wei Y,Qian Z,Guo G

更新日期：2015-01-01 00:00:00

abstract：:Implant surface topography influences osteoblastic proliferation, differentiation and extracellular matrix protein expressions. Studies on preliminary interactions of osteoblast-like cells on implant interface through in vitro systems, can give lucid insights to osseo-integrative efficacies of when in vivo implants. I...

journal_title：Biomaterials

authors： Jayaraman M,Meyer U,Bühner M,Joos U,Wiesmann HP

更新日期：2004-02-01 00:00:00

abstract：:Modeling the interaction between the supportive stroma and the hematopoietic stem and progenitor cells (HSPC) is of high interest in the regeneration of the bone marrow niche in blood disorders. In this work, we present an injectable co-culture system to study this interaction in a coherent in vitro culture and in viv...

journal_title：Biomaterials

authors： Tavakol DN,Tratwal J,Bonini F,Genta M,Campos V,Burch P,Hoehnel S,Béduer A,Alessandrini M,Naveiras O,Braschler T

更新日期：2020-02-01 00:00:00

abstract：:The present in vitro study investigates the cellular interaction of primary human osteoblasts with human and bovine solvent dehydrated cancellous bone (SDCB) discs. These are bio-implants from solvent dehydrated, gamma-irradiated preserved human and bovine cancellous bone, pre-treated to remove all cells, genetic comp...

journal_title：Biomaterials

authors： Trentz OA,Hoerstrup SP,Sun LK,Bestmann L,Platz A,Trentz OL

更新日期：2003-09-01 00:00:00

abstract：:Cationic polymer PEI-CyD is doped with Nd by plasma technology to produce the gene vector: Nd@PEI-CyD. Luciferase expression and EGFP transfection experiments performed in vitro reveal that Nd@PEI-CyD has significantly higher transfection efficiency than lipofectamine 2000 and PEI-CyD and the mechanism is studied and ...

journal_title：Biomaterials

authors： Wang Q,Jin W,Wu G,Zhao Y,Jin X,Hu X,Zhou J,Tang G,Chu PK

更新日期：2014-01-01 00:00:00

abstract：:Two kinds of micelles containing Rhodamine B were prepared by a solvent evaporation method. One (Lac-f-micelles: lactose-free micelles) was from a Rhodamine-containing copolymer, poly(ethylene glycol)-b-poly(l-lactide-co-2,2-dihydroxylmethyl-propylene carbonate/Rhodamine) [PEG(5000)-b-P(LA(4000)-co-DHP(600)/Rhodamine)...

journal_title：Biomaterials

authors： Ma P,Liu S,Huang Y,Chen X,Zhang L,Jing X

更新日期：2010-03-01 00:00:00

abstract：:Composite particles made by growing nanoscopic silicon wires from the surface of monodispersed, microsized silica beads were tested in this study for their ability to affect the integrity and permeability of an epithelial cell layer. Polyethylene glycol (PEG) is known to sterically stabilize particles and prevent prot...

journal_title：Biomaterials

authors： Uskoković V,Lee PP,Walsh LA,Fischer KE,Desai TA

更新日期：2012-02-01 00:00:00

abstract：:The regeneration of large bone defects remains an unsolved clinical problem, which could benefit from recent findings on the biology of skeletal stem and progenitor cells. The elucidation of conditions to specifically control their dynamic and function will likely enable the development of novel treatment strategies. ...

journal_title：Biomaterials

authors： Papageorgiou P,Vallmajo-Martin Q,Kisielow M,Sancho-Puchades A,Kleiner E,Ehrbar M

更新日期：2019-10-01 00:00:00

abstract：:Hematopoietic stem cells (HSCs) are the vital, life-long source of all blood cell types. They are found in stem cell niches, specific anatomic locations that offer all the factors and signals necessary for the maintenance of the stem cell potential of HSCs. Much attention has been paid to the biochemical composition o...

journal_title：Biomaterials

authors： Altrock E,Muth CA,Klein G,Spatz JP,Lee-Thedieck C

更新日期：2012-04-01 00:00:00

abstract：:Monocytes/Macrophages have long been recognized as key players in inflammation and wound healing and are often employed in vitro to gain an understanding of the inflammatory response to biomaterials. Previous work has demonstrated a drastic decrease in primary monocyte adherent density on biomaterial surfaces coupled ...

journal_title：Biomaterials

authors： Waldeck H,Wang X,Joyce E,Kao WJ

更新日期：2012-01-01 00:00:00

abstract：:Recent advances in materials development and tissue engineering has resulted in a substantial number of bioinspired materials that recapitulate cardinal features of bone extracellular matrix (ECM) such as dynamic inorganic and organic environment(s), hierarchical organization, and topographical features. Bone mimickin...

journal_title：Biomaterials

authors： Shih YV,Varghese S

更新日期：2019-04-01 00:00:00

abstract：:Neonates with congenital diaphragmatic hernia often require surgical defect closure with a patch. Alternatives to native diaphragmatic tissue are critically needed for this paediatric surgery. The clinical efficacy of mesh patches is limited by complications associated with residual foreign material and by hernia recu...

journal_title：Biomaterials

authors： Zhang XY,Yanagi Y,Sheng Z,Nagata K,Nakayama K,Taguchi T

更新日期：2018-06-01 00:00:00