Abstract:
:The present in vitro study investigates the cellular interaction of primary human osteoblasts with human and bovine solvent dehydrated cancellous bone (SDCB) discs. These are bio-implants from solvent dehydrated, gamma-irradiated preserved human and bovine cancellous bone, pre-treated to remove all cells, genetic components and water soluble proteins. Primary human osteoblasts were harvested from cancellous chips of trauma patients undergoing osteosynthesis with bone grafting from the iliac crest. All patients provided informed consent. The present investigation tested proliferation, synthesis of phenotypic marker, and morphology of primary cultured human osteoblasts on SDCB in vitro. The total protein and collagen type 1 content could not be revealed, due to the inherent naturally occurring protein content in these two bio-implants. In conclusion, our in vitro results suggest that SDCB may be a suitable bone substitute which provides a well structured and biocompatible scaffold for ingrowing human osteoblasts.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Trentz OA,Hoerstrup SP,Sun LK,Bestmann L,Platz A,Trentz OLdoi
10.1016/s0142-9612(03)00205-9keywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
3417-26issue
20eissn
0142-9612issn
1878-5905pii
S0142961203002059journal_volume
24pub_type
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