Abstract：
:Articular cartilage injuries do not heal and large defects result in osteoarthritis with major personal and socioeconomic costs. Osteochondral transplantation is an effective treatment for large joint defects but its use is limited by the inability to store cartilage for long periods of time. Cryopreservation/vitrification is one method to enable banking of this tissue but decades of research have been unable to successfully preserve the tissue while maintaining cartilage on its bone base - a requirement for transplantation. To address this limitation, human knee articular cartilage from total knee arthroplasty patients and deceased donors was exposed to specified concentrations of 4 different cryoprotective agents for mathematically determined periods of time at lowering temperatures. After complete exposure, the cartilage was immersed in liquid nitrogen for up to 3 months. Cell viability was 75.4 ± 12.1% determined by membrane integrity stains and confirmed with a mitochondrial assay and pellet culture documented production of sulfated glycosaminoglycans and collagen II similar to controls. This report documents successful vitrification of intact human articular cartilage on its bone base making it possible to bank this tissue indefinitely.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Jomha NM,Elliott JA,Law GK,Maghdoori B,Forbes JF,Abazari A,Adesida AB,Laouar L,Zhou X,McGann LEdoi
10.1016/j.biomaterials.2012.05.007subject
Has Abstractpub_date
2012-09-01 00:00:00pages
6061-8issue
26eissn
0142-9612issn
1878-5905pii
S0142-9612(12)00524-8journal_volume
33pub_type
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