Abstract：
:To broaden the clinical application of cartilage regenerative medicine, we should develop an implant-type tissue-engineered cartilage with firmness and 3-D structure. For that, we attempted to use a porous biodegradable polymer scaffold in the combination with atelocollagen hydrogel, and optimized the structure and composition of porous scaffold. We administered chondrocytes/atelocollagen mixture into the scaffolds with various kinds of porosities (80-95%) and pore sizes (0.3-2.0 mm), consisting of PLLA or related polymers (PDLA, PLA/CL and PLGA), and transplanted the constructs in the subcutaneous areas of nude mice. The constructs using scaffolds of excessively large pore sizes (>1 mm) broke out on the skin and impaired the host tissue. The scaffold with the porosity of 95% and pore size of 0.3 mm could effectively retain the cells/gel mixture and indicated a fair cartilage regeneration. Regarding the composition, the tissue-engineered cartilage was superior in PLGA and PLLA to that in PLA/CA and PDLA. The latter two showed the dense accumulation of macrophages, which may deteriorate the cartilage regeneration. Although PLGA or PLLA has been currently recommended for the scaffold of cartilage, the polymer for which biodegradation was exactly synchronized to the cartilage regeneration would improve the quality of the tissue-engineered cartilage.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Tanaka Y,Yamaoka H,Nishizawa S,Nagata S,Ogasawara T,Asawa Y,Fujihara Y,Takato T,Hoshi Kdoi
10.1016/j.biomaterials.2010.02.028subject
Has Abstractpub_date
2010-06-01 00:00:00pages
4506-16issue
16eissn
0142-9612issn
1878-5905pii
S0142-9612(10)00257-7journal_volume
31pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Substitution with carboxylic and benzylamine sulphonated groups conferred on dextran both antithrombic activity and the capacity to inhibit formation of the amplification C3 convertase of complement. In dextrans substituted with carboxylic groups (greater than 40%), a high content of sulphonate (greater than 10%) resu...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(85)90040-7
更新日期：1985-01-01 00:00:00
abstract：:Bone morphogenetic protein (BMP) 2-incorporated gelatin sponge is effective for in vivo osteoinduction. However, the modeling capacity of bone decreases with age. As atrial to stimulate effective bone formation for animals with decreased osteogenic potential, Wnt1 inducible signaling pathway protein (WISP) 1, an osteo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.04.035
更新日期：2011-08-01 00:00:00
abstract：:To enable the synthesis of hydrogels containing microporosity, a family of pore generating additives (porogens) were synthesized from poly(ethylene glycols) (PEGs) with different molecular weights using naphthyl acetic acid. The products formed were characterized by UV-vis, differential scanning calorimetry and soluti...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(93)90206-h
更新日期：1993-11-01 00:00:00
abstract：:Anchorage-dependent cells (NRK 49F) were grown at 37 degrees C in serum containing medium on glass coated with a N-isopropyl acrylamide/N-t-butyl acrylamide copolymer (NIPAaM/NTBAM) with a lower critical solution temperature (LCST) of 8 degrees C. After incubating the dishes and adherent cells at 4 degrees C for 1 h, ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(93)90230-y
更新日期：1993-01-01 00:00:00
abstract：:Microcomputed tomography (micro-CT) is increasingly being used to analyze the three-dimensional structure and architecture of microvascular networks. Therefore we have evaluated a micro-CT analysis of VEGF-induced vessel ingrowth into a porous polyurethane scaffold through comparison with analyses by CD31 immunohistoc...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.07.044
更新日期：2009-10-01 00:00:00
abstract：:Micro- and nano-scale intelligent devices can revolutionize the medical field. Although proteins are promising materials for creating biocompatible miniature medical devices with biological functions, construction of complicated solid-state architectures, using inherently vulnerable proteins, remains challenging. Here...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.10.045
更新日期：2018-02-01 00:00:00
abstract：:Transcription factors (TFs) can direct cell fate by binding to DNA and regulating gene transcription. Controlling the intracellular levels of specific TFs can therefore enable reprogramming of cellular function and differentiation. Direct delivery of recombinant TFs to target cells can thus have widespread therapeutic...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.04.012
更新日期：2012-07-01 00:00:00
abstract：:An increasing number of studies in cardiac cell therapy have provided encouraging results for cardiac repair. Adult stem cells may overcome ethical and availability concerns, with the additional advantages, in some cases, to allow autologous grafts to be performed. However, the major problems of cell survival, cell fa...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2012.04.028
更新日期：2012-08-01 00:00:00
abstract：:Biomimetic materials that target the immune system and generate an anti-tumor responses hold promise in augmenting cancer immunotherapy. These synthetic materials can be engineered and optimized for their biodegradability, physical parameters such as shape and size, and controlled release of immune-modulators. As thes...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.11.038
更新日期：2017-02-01 00:00:00
abstract：:The ability to precisely pattern embryonic stem (ES) cells in vitro into predefined arrays/geometries may allow for the recreation of a stem cell niche for better understanding of how cellular microenvironmental factors govern stem cell maintenance and differentiation. In this study, a new gelatin-based laser direct-w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.11.015
更新日期：2011-03-01 00:00:00
abstract：:Breast cancer is associated with high mortality due to tumor metastasis. The anti-metastasis efficacy of photochemotherapy is strictly limited by poor targeting capability with respect to circulating tumor cells (CTCs) in blood and lymph. Herein, we decorate the platelet membrane (PM) on a surface of nanoparticles (NP...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.03.024
更新日期：2019-06-01 00:00:00
abstract：:Hypoxia, as an inevitable characteristic of solid tumors, has been regarded as a noticeably causative factor to therapeutic resistance and metastatic variants. Exploring novel theranostics to realize the accurate diagnosis of hypoxia and the simultaneous implementation of effective therapy is a promising prospect for ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.05.018
更新日期：2018-08-01 00:00:00
abstract：:Intervertebral disc (IVD) herniation causes pain and disability, but current discectomy procedures alleviate pain without repairing annulus fibrosus (AF) defects. Tissue engineering strategies seal AF defects by utilizing hydrogel systems to prevent recurrent herniation, however current biomaterials are limited by poo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120309
更新日期：2020-11-01 00:00:00
abstract：:The use of a composite graft of bovine trabecular demineralized bone matrix (DBM) and perichondrium has been found a reliable method for in vivo generation of cartilage. In the present study, the mechanism whereby this commercially available matrix increases cartilage formation was investigated. First, the time course...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00165-3
更新日期：1999-02-01 00:00:00
abstract：:One of the most common non-viral methods for the introduction of foreign deoxyribonucleic acid (DNA) into cultured cells is calcium phosphate co-precipitate transfection. This technique involves the encapsulation of DNA within a calcium phosphate co-precipitate, particulate addition to in vitro cell culture, endocytos...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.09.042
更新日期：2004-06-01 00:00:00
abstract：:Multilayer nanofilms, formed by the layer-by-layer (LbL) adsorption of positively and negatively charged polyelectrolytes, are promising substrates for tissue engineering. We investigate here the attachment and function of hepatic cells on multilayer films in terms of film composition, terminal layer, rigidity, charge...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.06.027
更新日期：2008-10-01 00:00:00
abstract：:In order to assess the suitability of polymer tissue scaffolds for use in regenerative medicine, methods to characterise scaffolds are needed. This requires the scaffold's structure to be determined for which X-ray microscopic computed tomography (X-ray micro CT) is widely used. However, because scaffolds are generall...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.04.035
更新日期：2009-09-01 00:00:00
abstract：:Alginate hydrogels are used extensively in cell encapsulation, cell transplantation, and tissue engineering applications. Alginates possess many favorable properties required in biomaterials, but are unable to specifically interact with mammalian cells. We have therefore covalently modified alginate polysaccharides wi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(98)00107-0
更新日期：1999-01-01 00:00:00
abstract：:Improving the immunogenicity of subunit vaccines, in particular skewing of the immune response towards Th1 type immunity, is crucial for the development of effective vaccines against intracellular infections and for the development of anti-cancer vaccines. Small molecule TLR7/8 agonist hold high potential for this pur...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.03.026
更新日期：2018-09-01 00:00:00
abstract：:Tissue engineering in vitro and in vivo involves the interaction of cells with a material surface. The nature of the surface can directly influence cellular response, ultimately affecting the rate and quality of new tissue formation. Initial events at the surface include the orientated adsorption of molecules from the...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/0142-9612(96)85758-9
更新日期：1996-01-01 00:00:00
abstract：:Porous calcium phosphate ceramics consisting of hydroxyapatite (HA) and tricalcium phosphate (TCP) with different HA to TCP ratio were implanted intramuscularly in rabbits for six months in order to carry out a comparative study on osteogenic activity of the ceramics. Bone formation was detected only in HT73 (HA to TC...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00119-3
更新日期：2002-01-01 00:00:00
abstract：:Collagen, type I, is a highly abundant natural protein material which has been cross-linked by a variety of methods including chemical agents, physical heating and UV irradiation with the aim of enhancing its physical characteristics such as mechanical strength, thermal stability, resistance to proteolytic breakdown, ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.04.017
更新日期：2005-11-01 00:00:00
abstract：:Collagen fibers are essential components of tissues, which are highly conserved across the animal kingdom and could be extremely useful in tissue engineering. The formation of these macromolecular fibers depends on molecular interactions-based self-assembly of the basic building blocks of collagen called tropocollagen...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.06.074
更新日期：2011-10-01 00:00:00
abstract：:Capillary morphogenesis is a multistage, multicellular activity that plays a pivotal role in various developmental and pathological situations. In-depth understanding of the regulatory mechanism along with the capability of controlling the morphogenic process will have direct implications on tissue engineering and the...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.12.101
更新日期：2014-03-01 00:00:00
abstract：:Scanning electron microscopy has been used to study the interface between implants of pure metals and the muscular tissue of rats. Several problems arise in the interpretation of the observations made, due to the potential for disruption to the tissue and the exacerbation of any corrosion that may have occurred in viv...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(82)90006-0
更新日期：1982-07-01 00:00:00
abstract：:A nanohybrid vesicle was developed from cholesteryl succinyl silane(CSS) in combination of sol-gel process and self-assembly technique. The silicalike surface adds CSS vesicles remarkably high stability against destabilization in blood or leakage of drug cargos. It was found that CSS vesicles alone exhibited selective...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.08.043
更新日期：2011-12-01 00:00:00
abstract：:Hydrogels have been widely used as artificial cell niche to mimic extracellular matrix with tunable properties. However, changing biochemical cues in hydrogels developed-to-date would often induce simultaneous changes in mechanical properties, which do not support mechanistic studies on stem cell-niche interactions. H...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.11.064
更新日期：2014-02-01 00:00:00
abstract：:Hydrogels produced from self-assembling peptides and peptide derivatives are being investigated as synthetic extracellular matrices for defined cell culture substrates and scaffolds for regenerative medicine. In many cases, however, they are less stiff than the tissues and extracellular matrices they are intended to m...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.01.008
更新日期：2008-05-01 00:00:00
abstract：:We report a new template-directed method for the fabrication of hydroxyapatite (HAp) sponges by using amino-acid-coated HAp nanoparticles dispersed within a viscous polysaccharide (dextran sulfate) matrix, and describe the use of these materials for the viability and proliferation of human bone marrow stromal cells. T...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.04.037
更新日期：2005-11-01 00:00:00
abstract：:Based on the hydrophobic interaction with biomembranes, PFVYLI (PFV), a hydrophobic penetration peptide (HPP), was initially introduced to modify doxorubicin-loaded stealth-sustained liposomes (PFV-SSLs-DOX) against different breast cancer cell phenotypes irrespective of their receptor expression or antigen presence. ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.11.088
更新日期：2014-02-01 00:00:00
