Abstract：
:Polymers may serve as a biodegradable material in tissue engineering. To assess the biocompatibility of poly-beta-hydroxy butyric acid (PHB), we studied the structural organization of cellular molecules involved in adhesion using osteoblastic and epithelial cell lines. On PHB, both cell lines revealed a rounded cell shape due to reduced spreading. The filamentous organization of the actin cytoskeleton was impaired. In double immunofluorescence analyses we demostrated that the colocalization of the fibronectin fibrils with the actin filaments was lost in cultures on PHB. Similarly, collagen II distribution was altered, whereas the organization of collagen I was not obviously affected. Further evidence for impaired structural organization was obtained for the beta1-integrin receptor and vinculin which mediate the interaction of the cytoskeleton with the extracellular matrix. In confluent epithelial cells, the tight junction protein ZO-1 showed a larger lateral extension in the cell-cell contacts when cells were grown on PHB. Because structural organization of components which mediate cell-matrix and cell-cell adhesion controls cell physiology these parameters could be a sensitive indicator for the biocompatibility of implant materials.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Nebe B,Forster C,Pommerenke H,Fulda G,Behrend D,Bernewski U,Schmitz KP,Rychly Jdoi
10.1016/s0142-9612(00)00430-0keywords:
subject
Has Abstractpub_date
2001-09-01 00:00:00pages
2425-34issue
17eissn
0142-9612issn
1878-5905pii
S0142-9612(00)00430-0journal_volume
22pub_type
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