The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration.

Abstract:

:Tissue-engineering technology employing genetically-modified mesenchymal stem cells combined with proper scaffolds represents a promising strategy for bone regeneration. Elucidating the underlying mechanisms that govern the osteogenesis of mesenchymal stem cells will give deeper insights into the regulatory patterns, as well as provide more effective methods to enhance bone regeneration. In this study, miR-135 was identified as an osteogenesis-related microRNA that was up-regulated during the osteogenesis of rat adipose-derived stem cells (ADSCs). Gain- and loss-of-function experiments using a lentiviral expression system showed that Homeobox A2 (Hoxa2) was negatively regulated by miR-135, and luciferase reporter assay further indicated that miR-135 repressed Hoxa2 expression through binding to the 3'-untranslated region (3'-UTR) of the Hoxa2 mRNA. In vitro analyses showed that the overexpression of miR-135 significantly enhanced the expression of bone markers and extracellular matrix calcium deposition, whereas the knockdown of miR-135 suppressed these processes. Transduced ADSCs were then combined with poly(sebacoyl diglyceride) (PSeD) scaffold to repair a critical-sized calvarial defects in rats. The results showed that the overexpression of miR-135 significantly promoted new bone formation with higher bone mineral density (BMD) and number of trabeculae (Tb.N), as well as larger areas of newly formed bone and mineralization labeled by tetracycline, calcein and alizarin red. In contrast, the knockdown of miR-135 attenuated these processes. Additionally, immunohistochemical analyses showed that transduced ADSCs participated in new bone formation and a miR-135/Hoxa2/Runx2 pathway might contribute to the regulation of ADSC osteogenesis and bone regeneration. Taken together, our data suggested that miR-135 positively regulated the osteogenesis and bone regeneration of ADSCs both in vitro and in vivo. Thus, the combination of miR-135-modified ADSCs and the PSeD scaffold may serve as a promising and effective method to repair critical-sized bone defects.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Xie Q,Wang Z,Zhou H,Yu Z,Huang Y,Sun H,Bi X,Wang Y,Shi W,Gu P,Fan X

doi

10.1016/j.biomaterials.2015.10.042

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

279-294

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(15)00852-2

journal_volume

75

pub_type

杂志文章
  • Non-invasive characterization of structure and morphology of silk fibroin biomaterials using non-linear microscopy.

    abstract::Designing biomaterial scaffolds remains a major challenge in tissue engineering. Key to this challenge is improved understanding of the relationships between the scaffold properties and its degradation kinetics, as well as the cell interactions and the promotion of new matrix deposition. Here we present the use of non...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2007.12.049

    authors: Rice WL,Firdous S,Gupta S,Hunter M,Foo CW,Wang Y,Kim HJ,Kaplan DL,Georgakoudi I

    更新日期:2008-05-01 00:00:00

  • Capture of endothelial cells under flow using immobilized vascular endothelial growth factor.

    abstract::We demonstrate the ability of immobilized vascular endothelial growth factor (VEGF) to capture endothelial cells (EC) with high specificity under fluid flow. To this end, we engineered a surface consisting of heparin bound to poly-l-lysine to permit immobilization of VEGF through the C-terminal heparin-binding domain....

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2015.02.025

    authors: Smith RJ Jr,Koobatian MT,Shahini A,Swartz DD,Andreadis ST

    更新日期:2015-05-01 00:00:00

  • BBB leakage, astrogliosis, and tissue loss correlate with silicon microelectrode array recording performance.

    abstract::The clinical usefulness of brain machine interfaces that employ penetrating silicon microelectrode arrays is limited by inconsistent performance at chronic time points. While it is widely believed that elements of the foreign body response (FBR) contribute to inconsistent single unit recording performance, the relatio...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2015.02.081

    authors: Nolta NF,Christensen MB,Crane PD,Skousen JL,Tresco PA

    更新日期:2015-01-01 00:00:00

  • Quantification of the binding affinity of a specific hydroxyapatite binding peptide.

    abstract::The genesis of bone and teeth involves highly coordinated processes, which involve multiple cell types and proteins that direct the nucleation and crystallization of inorganic hydroxyapatite (HA). Recent studies have shown that peptides mediate the nucleation process, control HA microstructure or even inhibit HA miner...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.01.012

    authors: Weiger MC,Park JJ,Roy MD,Stafford CM,Karim A,Becker ML

    更新日期:2010-04-01 00:00:00

  • Disease-induced immunomodulation at biomaterial scaffolds detects early pancreatic cancer in a spontaneous model.

    abstract::Pancreatic cancer has the worst prognosis of all cancers due to disease aggressiveness and paucity of early detection platforms. We developed biomaterial scaffolds that recruit metastatic tumor cells and reflect the immune dysregulation of native metastatic sites. While this platform has shown promise in orthotopic br...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2020.120632

    authors: Bushnell GG,Orbach SM,Ma JA,Crawford HC,Wicha MS,Jeruss JS,Shea LD

    更新日期:2020-12-23 00:00:00

  • Dual-role self-assembling nanoplexes for efficient gene transfection and sustained gene delivery.

    abstract::Novel cationic pentablock copolymers with poly(diethylamino ethyl methacrylate) blocks covalently attached to parent triblock Pluronic copolymers have been designed and developed as sustained release non-viral gene delivery vectors. These copolymers electrostatically condense plasmid DNA into nanostructures (nanoplexe...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2007.10.010

    authors: Agarwal A,Unfer RC,Mallapragada SK

    更新日期:2008-02-01 00:00:00

  • Branched polyesters based on poly[vinyl-3-(dialkylamino)alkylcarbamate-co-vinyl acetate-co-vinyl alcohol]-graft-poly(d,l-lactide-co-glycolide): effects of polymer structure on cytotoxicity.

    abstract::Branched polyesters of the general structure poly[vinyl-3-(dialkylamino)alkylcarbamate-co-vinyl acetate-co-vinyl alcohol]-graft-poly(d,l-lactide-co-glycolide) have shown potential for nano- and micro-scale drug delivery systems. For further optimization of this polymer class their cytotoxicity needs to be characterize...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2006.12.002

    authors: Unger F,Wittmar M,Kissel T

    更新日期:2007-03-01 00:00:00

  • Biofunctionalized PEDOT-coated microfibers for the treatment of spinal cord injury.

    abstract::Poly(3, 4-ethylenedioxythiophene)-coated carbon microfibers (PEDOT-MFs) hold promise for developing advanced neuroprostheses and neural repair devices. We investigated the chronic cellular responses to PEDOT-MFs implanted into the uninjured and the transected rat spinal cord, and compared the effects of polymer surfac...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2016.02.037

    authors: Alves-Sampaio A,García-Rama C,Collazos-Castro JE

    更新日期:2016-05-01 00:00:00

  • A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering.

    abstract::The generation of effective tissue engineered bone grafts requires efficient exchange of nutrients and mechanical stimulus. Bioreactors provide a manner in which this can be achieved. We have recently developed a biaxial rotating bioreactor with efficient fluidics through in-silico modeling. Here we investigated its p...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.01.028

    authors: Zhang ZY,Teoh SH,Chong WS,Foo TT,Chng YC,Choolani M,Chan J

    更新日期:2009-05-01 00:00:00

  • Effect of water-soluble phospholipid polymers conjugated with papain on the enzymatic stability.

    abstract::To maintain enzymatic activity during long-term storage by conjugation with water-soluble 2-methacryloyloxyethyl phosphorylcholine (MPC) polymers (PMPC-COOH) having various molecular weights with a carboxylic group on the terminal, such compounds were synthesized as a polymer modifier using a photoinduced living radic...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(03)00474-5

    authors: Miyamoto D,Watanabe J,Ishihara K

    更新日期:2004-01-01 00:00:00

  • A multifunctional nanotheranostic for the intelligent MRI diagnosis and synergistic treatment of hypoxic tumor.

    abstract::Hypoxia, as an inevitable characteristic of solid tumors, has been regarded as a noticeably causative factor to therapeutic resistance and metastatic variants. Exploring novel theranostics to realize the accurate diagnosis of hypoxia and the simultaneous implementation of effective therapy is a promising prospect for ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2018.05.018

    authors: Song R,Zhang M,Liu Y,Cui Z,Zhang H,Tang Z,Chen X,Wu H,Yao Z,He M,Bu W

    更新日期:2018-08-01 00:00:00

  • Effect of tricaprin on the physical characteristics and in vitro release of etoposide from PLGA microspheres.

    abstract::The purpose of this article is to examine the effects of tricaprin on the physical characteristics and in vitro release of etoposide from poly (lactic-co-glycolic acid) microspheres. The microspheres were synthesized through the use of a single-emulsion solvent-extraction procedure. Samples from each batch of microsph...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(02)00053-4

    authors: Schaefer MJ,Singh J

    更新日期:2002-08-01 00:00:00

  • Biphasic scaffold for annulus fibrosus tissue regeneration.

    abstract::Intervertebral disc (IVD) degeneration is the major cause of lower back pain, while the currently available treatments are symptomatic rather than curative. Tissue engineering is a powerful therapeutic strategy that can restore the normal biomechanical motion of the human spine. The ability of a biphasic elastic scaff...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2007.10.031

    authors: Wan Y,Feng G,Shen FH,Laurencin CT,Li X

    更新日期:2008-02-01 00:00:00

  • Technological issues for the development of more efficient calcium phosphate bone cements: a critical assessment.

    abstract::The first calcium phosphate cements (CPCs) were discovered in the 1980s. Two decades later, the interest for these materials is still rising. The goal of the present document is to review the most recent achievements in the field and to analyze future directions in research and development. ...

    journal_title:Biomaterials

    pub_type: 杂志文章,评审

    doi:10.1016/j.biomaterials.2005.03.049

    authors: Bohner M,Gbureck U,Barralet JE

    更新日期:2005-11-01 00:00:00

  • Biomimetic and nanostructured hybrid bioactive glass.

    abstract::Inspired by nature's toughening mechanisms, we designed a new polyhedral oligomeric silsesquioxane (POSS)-derived hybrid glass (PHG) that has covalent interactions on the molecular scale between the inorganic POSS cage and organic phase. These features allow "elastic deformation" of the inorganic POSS cage in limited ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2015.01.024

    authors: Zhou X,Sahai N,Qi L,Mankoci S,Zhao W

    更新日期:2015-05-01 00:00:00

  • Investigation of surface properties of some polymers by a thermodynamic and mechanical approach: possibility of predicting mucoadhesion and biocompatibility.

    abstract::Mucoadhesive properties of several polymers, such as sodium alginate, hydroxypropylmethyl cellulose, scleroglucan, xanthan gum, polyacrylic acid (Carbopol), and poly co-(methyl vinyl ether-maleic anhydride) (Gantrez), have been investigated by comparing a thermodynamical and a mechanical approach. Surface properties o...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(94)90064-7

    authors: Esposito P,Colombo I,Lovrecich M

    更新日期:1994-02-01 00:00:00

  • Moderation of prekallkrein-factor XII interactions in surface activation of coagulation by protein-adsorption competition.

    abstract::Traditional biochemistry of contact activation of blood coagulation suggesting that anionic hydrophilic surfaces are specific activators of the cascade is inconsistent with known trends in protein adsorption. To investigate contact activation reactions, a chromogenic assay was used to measure prekallkrein (PK) hydroly...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.05.076

    authors: Chatterjee K,Thornton JL,Bauer JW,Vogler EA,Siedlecki CA

    更新日期:2009-10-01 00:00:00

  • The effect of hydrophilic chain length and iRGD on drug delivery from poly(ε-caprolactone)-poly(N-vinylpyrrolidone) nanoparticles.

    abstract::Poly(ε-caprolactone)-b-Poly(N-vinylpyrrolidone) (PCL-b-PVP) copolymers with different PVP block length were synthesized by xanthate-mediated reverse addition fragment transfer polymerization (RAFT) and the xanthate chain transfer agent on chain end was readily translated to hydroxy or aldehyde for conjugating various ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.08.072

    authors: Zhu Z,Xie C,Liu Q,Zhen X,Zheng X,Wu W,Li R,Ding Y,Jiang X,Liu B

    更新日期:2011-12-01 00:00:00

  • A computational modeling approach for enhancing self-assembly and biofunctionalisation of collagen biomimetic peptides.

    abstract::Collagen fibers are essential components of tissues, which are highly conserved across the animal kingdom and could be extremely useful in tissue engineering. The formation of these macromolecular fibers depends on molecular interactions-based self-assembly of the basic building blocks of collagen called tropocollagen...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.06.074

    authors: Krishnamoorthy N,Yacoub MH,Yaliraki SN

    更新日期:2011-10-01 00:00:00

  • Biodegradation, biocompatibility, and drug delivery in poly(ω-pentadecalactone-co-p-dioxanone) copolyesters.

    abstract::Poly(ω-pentadecalactone-co-p-dioxanone) [poly(PDL-co-DO)] copolyesters are copolymers of an isodimorphic system, which remain semicrystalline over the whole range of compositions. Here, we evaluated enzymatically synthesized poly(PDL-co-DO) copolymers as new materials for biomedical applications. In vivo experiments u...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.05.046

    authors: Liu J,Jiang Z,Zhang S,Liu C,Gross RA,Kyriakides TR,Saltzman WM

    更新日期:2011-09-01 00:00:00

  • Extraction of high quality RNA from polysaccharide matrices using cetyltrimethylammonium bromide.

    abstract::Polysaccharides are increasingly being used as biomaterials for tissue engineering and regenerative medicine. Quantitative analysis of gene expression from cells in three-dimensional (3D) scaffolds requires extraction of messenger RNA, which is complicated in polysaccharide materials by ionic complexing between nuclei...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.11.024

    authors: Wang L,Stegemann JP

    更新日期:2010-03-01 00:00:00

  • Porous methacrylate scaffolds: supercritical fluid fabrication and in vitro chondrocyte responses.

    abstract::This paper describes a method of foaming a polymer system comprising poly(ethyl methacrylate)/tetrahydrofurfuryl methacrylate (PEMA/THFMA), characterisation of the resulting porosity and use of the foam for chondrocyte culture. The potential for this polymer system to support cartilage repair has been investigated pre...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2003.10.023

    authors: Barry JJ,Gidda HS,Scotchford CA,Howdle SM

    更新日期:2004-08-01 00:00:00

  • A technique to immobilize bioactive proteins, including bone morphogenetic protein-4 (BMP-4), on titanium alloy.

    abstract::Immobilization of biomolecules on surfaces enables both localization and retention of molecules at the cell-biomaterial interface. Since metallic biomaterials used for orthopedic and dental implants possess a paucity of reactive functional groups, biomolecular modification of these materials is challenging. In the pre...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(01)00339-8

    authors: Puleo DA,Kissling RA,Sheu MS

    更新日期:2002-05-01 00:00:00

  • Macrophage response to cross-linked and conventional UHMWPE.

    abstract::To prevent wear debris-induced osteolysis and aseptic loosening, cross-linked ultra-high molecular weight polyethylene's (UHMWPE) with improved wear resistance have been developed. Hip simulator studies have demonstrated very low wear rates with these new materials leading to their widespread clinical use. However, th...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(03)00056-5

    authors: Sethi RK,Neavyn MJ,Rubash HE,Shanbhag AS

    更新日期:2003-07-01 00:00:00

  • Constitutive models for impacted morsellised cortico-cancellous bone.

    abstract::Constitutive models are developed, based on the results of confined compression testing, to describe the visco-elastic, and non-linear elasto-plastic behaviour of morsellised cortico-cancellous bone (MCB). It is found that the elastic modulus, E of MCB can be expressed as a linear function of the applied pressure, p. ...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2005.10.034

    authors: Phillips A,Pankaj P,May F,Taylor K,Howie C,Usmani A

    更新日期:2006-03-01 00:00:00

  • Thermosensitive hydrogels based on polypeptides for localized and sustained delivery of anticancer drugs.

    abstract::Thermosensitive hydrogels based on poly(γ-ethyl-L-glutamate)-poly(ethylene glycol)-poly(γ-ethyl-L-glutamate) triblock copolymers (PELG-PEG-PELG) were prepared for localized and sustained delivery of anticancer drugs. The polypeptide-based hydrogels showed much lower critical gelation concentration than the traditional...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2013.09.064

    authors: Cheng Y,He C,Ding J,Xiao C,Zhuang X,Chen X

    更新日期:2013-12-01 00:00:00

  • Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations, surface roughness, crystal structure and chemical composition.

    abstract::Titanium implants have been used widely and successfully for various types of bone-anchored reconstructions. It is believed that properties of oxide films covering titanium implant surfaces are of crucial importance for a successful osseointegration, in particular at compromized bone sites. The aim of the present stud...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(01)00131-4

    authors: Sul YT,Johansson CB,Petronis S,Krozer A,Jeong Y,Wennerberg A,Albrektsson T

    更新日期:2002-01-01 00:00:00

  • Cytotoxicity and MMP-9 activation induced in human mononuclear cells by UHMWPE oxidation.

    abstract::Ultra high molecular weight polyethylene (UHMWPE) used in orthopedic prosthesis can be oxidized during sterilization processes such as gamma-ray irradiation. Oxidation alters UHMWPE structure and mechanical properties and it has been suggested that this alteration is the first step in the loosening process. Direct eff...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(02)00097-2

    authors: Renò F,Bracco P,Costa L,Cannas M

    更新日期:2002-09-01 00:00:00

  • Magnetic nanoparticles encapsulated into biodegradable microparticles steered with an upgraded magnetic resonance imaging system for tumor chemoembolization.

    abstract::In this work, therapeutic magnetic micro carriers (TMMC) guided in real time by a magnetic resonance imaging (MRI) system are proposed as a mean to improve drug delivery to tumor sites. MRI steering constraints and physiological parameters for the chemoembolization of liver tumors were taken into account to design mag...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.08.005

    authors: Pouponneau P,Leroux JC,Martel S

    更新日期:2009-10-01 00:00:00

  • Effect of streptavidin affinity mutants on the integrin-independent adhesion of biotinylated endothelial cells.

    abstract::We have previously shown that the high-affinity streptavidin (SA)-biotin interaction enhanced the initial integrin-mediated adhesion of biotinylated endothelial cells to SA-coated surface by serving as an extrinsic bond to stabilize and enhance the intrinsic fibronectin-integrin binding between the cell and surface. H...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(02)00367-8

    authors: Chan BP,Chilkoti A,Reichert WM,Truskey GA

    更新日期:2003-02-01 00:00:00