The role of miR-135-modified adipose-derived mesenchymal stem cells in bone regeneration.


:Tissue-engineering technology employing genetically-modified mesenchymal stem cells combined with proper scaffolds represents a promising strategy for bone regeneration. Elucidating the underlying mechanisms that govern the osteogenesis of mesenchymal stem cells will give deeper insights into the regulatory patterns, as well as provide more effective methods to enhance bone regeneration. In this study, miR-135 was identified as an osteogenesis-related microRNA that was up-regulated during the osteogenesis of rat adipose-derived stem cells (ADSCs). Gain- and loss-of-function experiments using a lentiviral expression system showed that Homeobox A2 (Hoxa2) was negatively regulated by miR-135, and luciferase reporter assay further indicated that miR-135 repressed Hoxa2 expression through binding to the 3'-untranslated region (3'-UTR) of the Hoxa2 mRNA. In vitro analyses showed that the overexpression of miR-135 significantly enhanced the expression of bone markers and extracellular matrix calcium deposition, whereas the knockdown of miR-135 suppressed these processes. Transduced ADSCs were then combined with poly(sebacoyl diglyceride) (PSeD) scaffold to repair a critical-sized calvarial defects in rats. The results showed that the overexpression of miR-135 significantly promoted new bone formation with higher bone mineral density (BMD) and number of trabeculae (Tb.N), as well as larger areas of newly formed bone and mineralization labeled by tetracycline, calcein and alizarin red. In contrast, the knockdown of miR-135 attenuated these processes. Additionally, immunohistochemical analyses showed that transduced ADSCs participated in new bone formation and a miR-135/Hoxa2/Runx2 pathway might contribute to the regulation of ADSC osteogenesis and bone regeneration. Taken together, our data suggested that miR-135 positively regulated the osteogenesis and bone regeneration of ADSCs both in vitro and in vivo. Thus, the combination of miR-135-modified ADSCs and the PSeD scaffold may serve as a promising and effective method to repair critical-sized bone defects.






Xie Q,Wang Z,Zhou H,Yu Z,Huang Y,Sun H,Bi X,Wang Y,Shi W,Gu P,Fan X




Has Abstract


2016-01-01 00:00:00












  • Hyaluronic acid-shelled acid-activatable paclitaxel prodrug micelles effectively target and treat CD44-overexpressing human breast tumor xenografts in vivo.

    abstract::The therapeutic efficacy of nanoscale anticancer drug delivery systems is severely truncated by their low tumor-targetability and inefficient drug release at the target site. Here, we report the design and development of novel endosomal pH-activatable paclitaxel prodrug micelles based on hyaluronic acid-b-dendritic ol...


    pub_type: 杂志文章


    authors: Zhong Y,Goltsche K,Cheng L,Xie F,Meng F,Deng C,Zhong Z,Haag R

    更新日期:2016-04-01 00:00:00

  • Neurite outgrowth on well-characterized surfaces: preparation and characterization of chemically and spatially controlled fibronectin and RGD substrates with good bioactivity.

    abstract::Study of axonal growth and ligand-receptor interactions requires specificity and careful characterization of the biomaterial substrates to which the neurons bind. It would be impossible to predict the effects of important variables such as composition, surface density, spatial distribution, and conformation of the lig...


    pub_type: 杂志文章


    authors: Zhang Z,Yoo R,Wells M,Beebe TP Jr,Biran R,Tresco P

    更新日期:2005-01-01 00:00:00

  • Hierarchical targeted hepatocyte mitochondrial multifunctional chitosan nanoparticles for anticancer drug delivery.

    abstract::The overwhelming majority of drugs exert their pharmacological effects after reaching their target sites of action, however, these target sites are mainly located in the cytosol or intracellular organelles. Consequently, delivering drugs to the specific organelle is the key to achieve maximum therapeutic effects and m...


    pub_type: 杂志文章


    authors: Chen Z,Zhang L,Song Y,He J,Wu L,Zhao C,Xiao Y,Li W,Cai B,Cheng H,Li W

    更新日期:2015-06-01 00:00:00

  • Three-dimensional, soft neotissue arrays as high throughput platforms for the interrogation of engineered tissue environments.

    abstract::Local signals from tissue-specific extracellular matrix (ECM) microenvironments, including matrix adhesive ligand, mechanical elasticity and micro-scale geometry, are known to instruct a variety of stem cell differentiation processes. Likewise, these signals converge to provide multifaceted, mechanochemical cues for h...


    pub_type: 杂志文章


    authors: Floren M,Tan W

    更新日期:2015-08-01 00:00:00

  • Structural modifications and biological compatibility of doped bio-active glasses.

    abstract::The Raman laser and infrared spectra of doped bio-active glasses of the 45S5 type are presented and discussed. The spectroscopic results show that the doping agents cause the destruction of the basic glass structure and the consequent formation of SiO4(4-) units in the glass network. When the doped glasses have been i...


    pub_type: 杂志文章


    authors: Krajewski A,Ravaglioli A,Bertoluzza A,Monti P,Battaglia MA,Pizzoferrato A,Olmi R,Moroni A

    更新日期:1988-11-01 00:00:00

  • Intraperitoneal delivery of platinum with in-situ crosslinkable hyaluronic acid gel for local therapy of ovarian cancer.

    abstract::Intraperitoneal (IP) chemotherapy is a promising post-surgical therapy of solid carcinomas confined within the peritoneal cavity, with potential benefits in locoregional and systemic management of residual tumors. In this study, we intended to increase local retention of platinum in the peritoneal cavity over a prolon...


    pub_type: 杂志文章


    authors: Cho EJ,Sun B,Doh KO,Wilson EM,Torregrosa-Allen S,Elzey BD,Yeo Y

    更新日期:2015-01-01 00:00:00

  • Layered self-assemblies for controlled drug delivery: A translational overview.

    abstract::Self-assembly is a prominent phenomenon observed in nature. Inspired by this thermodynamically favorable approach, several natural and synthetic materials have been investigated to develop functional systems for various biomedical applications, including drug delivery. Furthermore, layered self-assembled systems provi...


    pub_type: 杂志文章,评审


    authors: Sarode A,Annapragada A,Guo J,Mitragotri S

    更新日期:2020-02-29 00:00:00

  • Amphiphilic multiarm star block copolymer-based multifunctional unimolecular micelles for cancer targeted drug delivery and MR imaging.

    abstract::We report on the fabrication of multifunctional polymeric unimolecular micelles as an integrated platform for cancer targeted drug delivery and magnetic resonance imaging (MRI) contrast enhancement under in vitro and in vivo conditions. Starting from a fractionated fourth-generation hyperbranched polyester (Boltorn H4...


    pub_type: 杂志文章


    authors: Li X,Qian Y,Liu T,Hu X,Zhang G,You Y,Liu S

    更新日期:2011-09-01 00:00:00

  • Differential and synergistic effects of mechanical stimulation and growth factor presentation on vascular wall function.

    abstract::We investigated the hypothesis that immobilizing TGF-β1 within fibrin hydrogels may act in synergy with cyclic mechanical stimulation to enhance the properties of vascular grafts. To this end, we engineered a fusion TGF-β1 protein that can covalently anchor to fibrin during polymerization upon the action of factor XII...


    pub_type: 杂志文章


    authors: Liang MS,Koobatian M,Lei P,Swartz DD,Andreadis ST

    更新日期:2013-10-01 00:00:00

  • Hydroxyapatite-polyethylene composites for bone substitution: effects of ceramic particle size and morphology.

    abstract::Synthetic hydroxyapatite particles of two median sizes and different morphologies have been used to manufacture hydroxyapatite reinforced high density polyethylene composites (HAPEX) for medical applications. The effects of hydroxyapatite particle size on properties of the resultant composites were investigated using ...


    pub_type: 杂志文章


    authors: Wang M,Joseph R,Bonfield W

    更新日期:1998-12-01 00:00:00

  • The effect of photopolymerization on stem cells embedded in hydrogels.

    abstract::Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator...


    pub_type: 杂志文章


    authors: Fedorovich NE,Oudshoorn MH,van Geemen D,Hennink WE,Alblas J,Dhert WJ

    更新日期:2009-01-01 00:00:00

  • SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics.

    abstract::Surface plasmon resonance (SPR) sensing has long been used to study biomolecular binding events and their kinetics in a label-free way. This approach has recently been extended to SPR microscopy, which is an ideal tool for probing large microarrays of biomolecules for their binding interactions with various partners a...


    pub_type: 杂志文章,评审


    authors: Campbell CT,Kim G

    更新日期:2007-05-01 00:00:00

  • Multifunctional rare-earth self-assembled nanosystem for tri-modal upconversion luminescence /fluorescence /positron emission tomography imaging.

    abstract::Rare-earth upconversion nanoparticles (UCNP) which can absorb low-energy photons and emit high energy photons have attracted great interest not only because of their unique application in upconversion luminescence imaging, but also because they can be used as ideal building blocks for multimodal bioimaging probes. Imp...


    pub_type: 杂志文章


    authors: Liu Q,Chen M,Sun Y,Chen G,Yang T,Gao Y,Zhang X,Li F

    更新日期:2011-11-01 00:00:00

  • Cancer stem cell labeling using poly(L-lysine)-modified iron oxide nanoparticles.

    abstract::Cell labeling using magnetic nanoparticles is an increasingly used approach in noninvasive behavior tracking, in vitro separation of cancer stem cells (CSCs), and CSC-based research in cancer therapy. However, the impact of magnetic labeling on the biological properties of targeted CSCs, such as self-renewal, prolifer...


    pub_type: 杂志文章


    authors: Wang X,Wei F,Liu A,Wang L,Wang JC,Ren L,Liu W,Tu Q,Li L,Wang J

    更新日期:2012-05-01 00:00:00

  • Binding of corroded ions to human saliva.

    abstract::Employing equilibrium dialysis, the binding abilities of Cu, Al, Co and Cr ions from corroded Cu-Al and Co-Cr dental casting alloys towards human saliva and two of its gel chromatographic fractions were determined. Results indicate that both Cu and Co bind to human saliva i.e. 0.045 and 0.027 mg/mg protein, respective...


    pub_type: 杂志文章


    authors: Mueller HJ

    更新日期:1985-05-01 00:00:00

  • In vitro engineering of fibrocartilage using CDMP1 induced dermal fibroblasts and polyglycolide.

    abstract::This study was designed to explore the feasibility of using cartilage-derived morphogenetic protein-1 (CDMP1) induced dermal fibroblasts (DFs) as seed cells and polyglycolide (PGA) as scaffold for fibrocartilage engineering. DFs isolated from canine were expanded and seeded on PGA scaffold to fabricate cell/scaffold c...


    pub_type: 杂志文章


    authors: Zhao G,Yin S,Liu G,Cen L,Sun J,Zhou H,Liu W,Cui L,Cao Y

    更新日期:2009-07-01 00:00:00

  • Mechanisms by which the inhibition of specific intracellular signaling pathways increase osteoblast proliferation on apatite surfaces.

    abstract::Osteoblasts proliferate slowly on the surface of calcium phosphate apatite which is widely used as a substrate biomaterial in bone regeneration. Owing to poor adhesion signaling in the cells grown on the calcium phosphate surface, inadequate growth factor signaling is generated to trigger cell cycle progression. The p...


    pub_type: 杂志文章


    authors: Yang S,Tian YS,Lee YJ,Yu FH,Kim HM

    更新日期:2011-04-01 00:00:00

  • Temporally degradable collagen-mimetic hydrogels tuned to chondrogenesis of human mesenchymal stem cells.

    abstract::Tissue engineering strategies for repairing and regenerating articular cartilage face critical challenges to recapitulate the dynamic and complex biochemical microenvironment of native tissues. One approach to mimic the biochemical complexity of articular cartilage is through the use of recombinant bacterial collagens...


    pub_type: 杂志文章


    authors: Parmar PA,Skaalure SC,Chow LW,St-Pierre JP,Stoichevska V,Peng YY,Werkmeister JA,Ramshaw JA,Stevens MM

    更新日期:2016-08-01 00:00:00

  • An 'in vitro' study of mechanical fatigue in glutaraldehyde-treated porcine aortic valve tissue.

    abstract::Leaflet tissue specimens prepared from porcine aortic valves treated with glutaraldehyde at low and high pressures have been subjected to 0.45 x 10(9) accelerated fatigue cycles in Ringer's solution. The waveform or crimped property of the collagen fibres in the leaflet tissue is an essential requirement for its abili...


    pub_type: 杂志文章


    authors: Broom ND

    更新日期:1980-01-01 00:00:00

  • Characterization of interfaces in human calcified tissue.

    abstract::Thermostimulated current spectroscopy has been applied to the investigation of molecular mobility in human calcified tissue. A comparative study of extracts at various stages of demineralization is presented. The response of an organic-mineral complex interphase has been identified. ...


    pub_type: 杂志文章


    authors: Martin M,Lamure A,Lacabanne C,Betin C,Harmand MF

    更新日期:1990-07-01 00:00:00

  • Acidity-triggered charge-convertible nanoparticles that can cause bacterium-specific aggregation in situ to enhance photothermal ablation of focal infection.

    abstract::Focal infections that are caused by antibiotic-resistant bacteria are becoming an ever-growing challenge to human health. To address this challenge, a pH-responsive amphiphilic polymer of polyaniline-conjugated glycol chitosan (PANI-GCS) that can self-assemble into nanoparticles (NPs) in situ is developed. The PANI-GC...


    pub_type: 杂志文章


    authors: Korupalli C,Huang CC,Lin WC,Pan WY,Lin PY,Wan WL,Li MJ,Chang Y,Sung HW

    更新日期:2017-02-01 00:00:00

  • Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.

    abstract::Ocular surface diseases including conjunctival disorders are multifactorial progressive conditions that can severely affect vision and quality of life. In recent years, stem cell therapies based on conjunctival stem cells (CjSCs) have become a potential solution for treating ocular surface diseases. However, neither a...


    pub_type: 杂志文章


    authors: Zhong Z,Deng X,Wang P,Yu C,Kiratitanaporn W,Wu X,Schimelman J,Tang M,Balayan A,Yao E,Tian J,Chen L,Zhang K,Chen S

    更新日期:2021-01-01 00:00:00

  • Mass preparation of size-controlled mouse embryonic stem cell aggregates and induction of cardiac differentiation by cell patterning method.

    abstract::Embryonic stem cells (ESCs) are promising cell sources for cell-based therapy. It has been established that the formation of ESC aggregates promotes their differentiation into the derivatives of all three germ layers. ESC aggregates are generally prepared via the formation of suspended spherical aggregates called embr...


    pub_type: 杂志文章


    authors: Sasaki D,Shimizu T,Masuda S,Kobayashi J,Itoga K,Tsuda Y,Yamashita JK,Yamato M,Okano T

    更新日期:2009-09-01 00:00:00

  • Computer graphics of hydroxyapatite and beta-tricalcium phosphate.

    abstract::Hydroxyapatite (HA) and beta-tricalcium phosphate (TCP) were studied by computer graphics with a personal computer. Crystal models of fluoridated hydroxyapatite and fluorapatite, in which F- ions are substituted into some or all OH- positions, were also drawn. Data on the structural coordinates of the hydroxyapatite a...


    pub_type: 杂志文章


    authors: Okazaki M,Sato M

    更新日期:1990-10-01 00:00:00

  • Biomaterial-induced alterations of human neutrophils under fluid shear stress: scanning electron microscopical study in vitro.

    abstract::Morphological changes of human polymorphonuclear neutrophils (PMN) adhering to hydrophilic (glass) and hydrophobic (FEP-Teflon, polyethylene, polypropylene) surfaces were studied in a parallel-plate flow chamber at the light and scanning electron microscopical levels. The PMN were exposed to a shear stress of 0.19 Pa ...


    pub_type: 杂志文章


    authors: Tomczok J,Sliwa-Tomczok W,Klein CL,van Kooten TG,Kirkpatrick CJ

    更新日期:1996-07-01 00:00:00

  • Gel-impregnated pore membranes with mesh-size asymmetry for biohybrid artificial organs.

    abstract::Membranes based on mechanically supported poly(vinyl alcohol) (PVA) hydrogels with mesh-size asymmetry were developed for potential application in biohybrid artificial organs. The pores of cellulose ester microfiltration membranes were impregnated with a PVA solution, which was lightly crosslinked with glutaraldehyde ...


    pub_type: 杂志文章


    authors: Dai WS,Barbari TA

    更新日期:2000-07-01 00:00:00

  • Prion inhibition with multivalent PrPSc binding compounds.

    abstract::Quinacrine and related heterocyclic compounds have antiprion activity. Since the infectious pathogen of prion diseases is composed of multimeric PrP(Sc) assemblies, we hypothesized that this antiprion property could be enhanced by attaching multiple quinacrine-derived chloroquinoline or acridine moieties to a scaffold...


    pub_type: 杂志文章


    authors: Mays CE,Joy S,Li L,Yu L,Genovesi S,West FG,Westaway D

    更新日期:2012-10-01 00:00:00

  • Physical and chemical processing for a human dura mater substitute.

    abstract:OBJECT:Allogenic human fascia lata used in neurosurgery, as dura mater substitute, can be associated with a risk of viral and bacterial transmission. Chemical and physical procedures, developed to inactivate virus and bacteria, have been applied to fascia lata. The aim of this study consists in the evaluation of the bi...


    pub_type: 杂志文章


    authors: Dufrane D,Cornu O,Delloye C,Schneider YJ

    更新日期:2002-07-01 00:00:00

  • Nanopatterned muscle cell patches for enhanced myogenesis and dystrophin expression in a mouse model of muscular dystrophy.

    abstract::Skeletal muscle is a highly organized tissue in which the extracellular matrix (ECM) is composed of highly-aligned cables of collagen with nanoscale feature sizes, and provides structural and functional support to muscle fibers. As such, the transplantation of disorganized tissues or the direct injection of cells into...


    pub_type: 杂志文章


    authors: Yang HS,Ieronimakis N,Tsui JH,Kim HN,Suh KY,Reyes M,Kim DH

    更新日期:2014-02-01 00:00:00

  • Analgesic drug delivery via recombinant tissue plasminogen activator and microRNA-183-triggered opening of the blood-nerve barrier.

    abstract::The peripheral nerve contains three barriers which include the blood-nerve barrier consisting of endoneurial vessels and the perineurium as well as autotypic junctions in Schwann cells. The perineurium prevents diffusion of perineurally injected drugs that can be used for selective regional pain control. It is compose...


    pub_type: 杂志文章


    authors: Yang S,Krug SM,Heitmann J,Hu L,Reinhold AK,Sauer S,Bosten J,Sommer C,Fromm M,Brack A,Rittner HL

    更新日期:2016-03-01 00:00:00