Abstract:
:The neutrophil polarization assay, a technique used to measure chemotaxis, was adapted to examine complement activation. Complement activation in serum which had been incubated with metallic and polymeric biomaterials was examined using the neutrophil polarization assay and immunoelectrophoresis assay. In agreement with previous publications, nylon activated the complement cascade, but PTFE did not. The neutrophil polarization assay was found to be the most sensitive technique for examining complement activation by endotoxin but the immunoelectrophoresis assay is the technique most sensitive for detecting complement activation by cobalt powder. In both assays, complement activation was not detected in serum incubated with chromium powder. However, serum incubated with silver and nickel powder stimulated neutrophils to polarize indicating that these powders may activate complement.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Remes A,Williams DFdoi
10.1016/0142-9612(91)90060-nsubject
Has Abstractpub_date
1991-08-01 00:00:00pages
607-13issue
6eissn
0142-9612issn
1878-5905pii
0142-9612(91)90060-Njournal_volume
12pub_type
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