Abstract:
:Polyacrylamide hydrogels have been used extensively to study cell responses to the mechanical and biochemical properties of extracellular matrix substrates. A key step in fabricating these substrates is the conjugation of cell adhesion proteins to the polyacrylamide surfaces, which typically involves nonspecifically anchoring these proteins via side-chain functional groups. This can result in a loss of presentation control and altered bioactivity. Here, we describe a new functionalization strategy in which we anchor full-length extracellular matrix proteins to polyacrylamide substrates using 2-pyridinecarboxaldehyde, which can be co-polymerized into polyacrylamide gels and used to immobilize proteins by their N-termini. This one-step reaction proceeds under mild aqueous conditions and does not require additional reagents. We demonstrate that these substrates can readily conjugate to various extracellular matrix proteins, as well as promote cell adhesion and spreading. Notably, this chemistry supports the assembly and cellular remodeling of large collagen fibers, which is not observed using conventional side-chain amine-conjugation chemistry.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Lee JP,Kassianidou E,MacDonald JI,Francis MB,Kumar Sdoi
10.1016/j.biomaterials.2016.06.022subject
Has Abstractpub_date
2016-09-01 00:00:00pages
268-76eissn
0142-9612issn
1878-5905pii
S0142-9612(16)30276-9journal_volume
102pub_type
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