Abstract：
:In this study, we investigate the molecular mechanisms by which human osteoblasts (HOB) challenged with wear debris promote the differentiation of osteoclast precursors. HOB were obtained from trabecular bone and exposed to alumina (Al(2)O(3)) or 'ultra-high molecular weight polyethylene' (UHMWPE) particles for 24h. The supernatant (HOB-CM) was used for the immunoenzymatic detection of receptor activator of NF-kappaB ligand (RANKL) and osteoprotegerin (OPG), as well as for inducing the osteoclast differentiation from peripheral blood mononuclear cells (PBMC). The OPG-to-RANKL ratio was significantly decreased in the conditioned medium of UHMWPE-challenged HOB. Morphological and cytochemical analysis showed that HOB-CM induced by itself the osteoclast formation, but a large amount of multinucleated tartrate-resistant acid phosphatase (TRAP)-positive giant cells were obtained when PBMCs were cultured with 1 microg/mL UHMWPE HOB-CM. The expression of genes involved in osteoclast differentiation and activation was evaluated, i.e. c-fms, RANK, c-src, c-fos, cathepsin-K (CATK), TRAP, and calcitonin R (CTR). The UHMWPE HOB-CM increases c-src expression, suggesting that polyethylene debris favour the paracrine activity of HOB in inducing the pathway involved in osteoclast polarization and adhesion. On the contrary, Al(2)O(3) HOB-CM downregulates c-fos expression, suggesting that the passage from macrophages into the osteoclast lineage is deviated. These results show that Al(2)O(3) wear debris is less active than UHMWPE in inducing osteoclast differentiation. Moreover, they provide new insight into the molecular basis of particle-induced osteoclastogenesis, that is the starting point for planning mode-specific targeting of periprosthetic osteolysis.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Granchi D,Amato I,Battistelli L,Ciapetti G,Pagani S,Avnet S,Baldini N,Giunti Adoi
10.1016/j.biomaterials.2004.07.045keywords:
subject
Has Abstractpub_date
2005-05-01 00:00:00pages
2371-9issue
15eissn
0142-9612issn
1878-5905pii
S0142-9612(04)00697-0journal_volume
26pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Human embryonic stem cells (hESCs) are generally induced to differentiate by forming spherical structures termed embryoid bodies (EBs) in the presence of soluble growth factors. hEBs are generated by suspending small clumps of hESC colonies; however, the resulting hEBs are heterogeneous because this method lacks the a...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.04.001
更新日期：2014-07-01 00:00:00
abstract：:6-O-Carboxymethylchitin (CM-chitin) was partially deacetylated by alkaline treatment, and the 30% deacetylated product was named CM-DA30 and used for the preparation of microspheres. An aqueous solution of CM-DA30 was added to hexane containing 1% (w/w) sorbitan sesquioleate and emulsified by stirring and sonication, ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00069-7
更新日期：2000-09-01 00:00:00
abstract：:Reactive oxygen species (ROS) have been shown to play crucial roles in regulating various cellular functions, e.g. focal adhesion (FA) dynamics and cell migration upon growth factor stimulation. However, it is not clear how ROS are regulated at subcellular FA sites to impact cell migration. We have developed a biosens...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.01.092
更新日期：2013-05-01 00:00:00
abstract：:Photodynamic therapy (PDT) is a new therapeutic approach for the palliative treatment of malignant bile duct obstruction. In this study, we designed photosensitizer-embedded self-expanding nonvascular metal stent (PDT-stent) which allows repeatable photodynamic treatment of cholangiocarcinoma without systemic injectio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.07.001
更新日期：2014-10-01 00:00:00
abstract：:The differences in morphological behaviour between fibroblasts cultured on smooth and nanogrooved substrata (groove depth: 5-350 nm, width: 20-1000 nm) have been evaluated in vitro. The aim of the study was to clarify to what extent cell guidance occurs on increasingly smaller topographies. Pattern templates were made...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.05.030
更新日期：2007-09-01 00:00:00
abstract：:The elasticity, mechanical strength and permeability of a new biomaterial made of elastin and fibrin were investigated. It was shown that gamma-irradiation to sterilize the product, and sulphur derivatives (Merceptyl, thiourea, cystein) working as reticulating agents modified these properties. Depending on whether or ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(89)90084-7
更新日期：1989-09-01 00:00:00
abstract：:Hydroxyapatite ceramics with zirconia dispersion from fine powders synthesized hydrothermally were post-sintered at 1000-1300 degrees C under 200 MPa of argon for 1 h without capsules, after normal sintering in air at 1200 degrees C for 3 h. Densification was most significant with post-sintering at 1200 degrees C. Fra...
journal_title：Biomaterials
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:The metastatic spread of cancer cells from the primary tumor to distant sites leads to a poor prognosis in cancers originating from multiple organs. Increasing evidence has linked selectin-based adhesion between circulating tumor cells (CTCs) and endothelial cells of the microvasculature to metastatic dissemination, i...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.03.045
更新日期：2015-07-01 00:00:00
abstract：:The adsorption of bovine serum albumin (BSA) on to titanium powder has been studied as a function of protein concentration and pH, and in the presence of calcium and phosphate ions. Isotherm data have shown that the adsorption process does not follow the Langmuir model (inflection points). The maximum adsorption (Adma...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)83280-7
更新日期：1996-05-01 00:00:00
abstract：:Tissue engineering combined with gene therapy represents a promising approach for bone regeneration. The Hypoxia-inducible factor-1α (HIF-1α) gene is a pivotal regulator of vascular reactivity and angiogenesis. Our recent study has showed that HIF-1α could promote osteogenesis of bone mesenchymal stem cells (BMSCs) us...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.09.005
更新日期：2011-12-01 00:00:00
abstract：:Recent developments in cell culture techniques have made it possible to study the cellular mechanisms involved in carcinogenesis and to apply these methods as screening tools in vitro. This study investigated and compared the ability of the metals most commonly used in orthopedic implants to induce toxicity and neopla...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00209-3
更新日期：1998-04-01 00:00:00
abstract：:We have previously observed that muscle-derived stem cells (MDSC) seeded onto porcine small intestinal submucosa (SIS) increase the mechanical compliance of the engineered tissue construct [Lu SH, Sacks MS, Chung SY, Gloeckner DC, Pruchnic R, Huard J, et al. Biaxial mechanical properties of muscle-derived cell seeded ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.10.011
更新日期：2006-04-01 00:00:00
abstract：:The authors aimed to develop a liquid material for embolization of aneurysms. In vitro and in vivo performances of the new embolic material were examined by cell culture and using an aneurysm model made in common carotid arteries (CCAs) of adult rats. Engineered protein ProNectin F (PnF), which contains 13 sites of an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.10.021
更新日期：2004-08-01 00:00:00
abstract：:Tropoelastin, the polypeptide monomer precursor of elastin, is covalently cross-linked to give stable elastic structures. We show here that elastic biomaterials can be generated from tropoelastin in the absence of the classically accepted cross-linking pathway. Under alkaline conditions tropoelastin proceeds through a...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.10.018
更新日期：2009-02-01 00:00:00
abstract：:A major hindrance in islet transplantation as a feasible therapeutic approach for patients with type 1 diabetes is the insufficient oxygenation of the grafts, which results in cell death in portions of the implant. Here we address this limitation through the application of oxygen-generating microparticles (MP) and a f...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.02.043
更新日期：2016-05-01 00:00:00
abstract：:An in vitro cell-implant mineralization model system was used to study the effect of transforming growth factor-beta 1(TGF-beta1) on mineralization in human osteoblast cultures. SaOs-2 and primary human osteoblast (HOB) cells were cultured on Tivanium (Ti-6Al-4V) disks. Administration of different concentrations of TG...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00616-6
更新日期：2003-05-01 00:00:00
abstract：:Microspheres of a hydrophobic and a hydrophilic poly(ether-ester) copolymer were evaluated for their in vitro and in vivo biocompatibility and degradation. The microspheres prior to and after sterilization were tested for in vitro cytotoxicity. The in vivo biocompatibility of the poly(ethylene glycol) terephthalate an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00220-x
更新日期：2002-12-01 00:00:00
abstract：:A graft copolymer having poly(L-lysine) (PLL) as the backbone and monomethoxy poly(ethylene glycol) (MPEG) as pendent chains was synthesized. This polycationic copolymer was used to form microcapsules with sodium alginate, a polyanion. Microcapsules and model surfaces formed with PLL-graft-MPEG demonstrated reduced pr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(92)90180-v
更新日期：1992-01-01 00:00:00
abstract：:A variety of techniques have been applied to generate tissue engineered constructs, where cells are combined with degradable scaffolds followed by a period of in vitro culture or direct implantation. In the current study, a cellularized scaffold was generated by concurrent deposition of electrospun biodegradable elast...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.10.029
更新日期：2017-01-01 00:00:00
abstract：:Dense, reconstituted collagen membranes, using an optimized cross-linking protocol, were implanted subcutaneously into rats. After implantation, only a mild inflammatory response in the surrounding tissues was observed. The morphology of the fibroblasts around the membranes was seen to go through characteristic stages...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(95)98905-t
更新日期：1995-09-01 00:00:00
abstract：:Loosening of the femoral component in a total hip arthroplasty with concomitant bone loss can pose a problem for revision surgery due to inadequate structure in the remaining femur. While impaction allografting has shown promise, it has also shown serious complications, especially with moderate to severe bone loss. It...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.05.062
更新日期：2005-12-01 00:00:00
abstract：:A-B-type block copolymers, consisting of polystyrene (PST) or poly(methyl methacrylate) (PMMA) forming segment (A) and poly(gamma-benzyl L-glutamate) (P[Glu(OBzl)]) segment (B), were synthesized and the thrombus formation on these block copolymer films was investigated in relation to the adsorption of plasma proteins ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(86)90010-4
更新日期：1986-09-01 00:00:00
abstract：:Endothelial cells derived from the bovine pulmonary artery (line CPAE, CCL 209, American Tissue Culture Collection, Rockville, MD, USA) were cultured on pristine or fluorine ion-irradiated polystyrene (5 x 10(12) or 5 x 10(14) F ions/cm2, 150 keV). At 24-h post-seeding interval, the number of cells which adhered to th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00009-0
更新日期：2000-06-01 00:00:00
abstract：:Carbon nanotubes have shown great potential in various areas of biomedicine. Herein, we synthesize a series of amphiphilic polymers by anchoring polyethylene glycol (PEG) of different lengths at various densities on poly(maleic anhydride-alt-1-octadecene) (PMHC(18)). The blood circulation and biodistribution of those ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.08.096
更新日期：2011-01-01 00:00:00
abstract：:The water absorption characteristics of resins, upon which many composite filling materials are based, have been studied. In general, the equilibrium uptake is less than that predicted from the water absorption of composite filling materials, if it is assumed that the polymer alone absorbs the water. Also, whereas the...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(86)90039-6
更新日期：1986-11-01 00:00:00
abstract：:Intervertebral disc (IVD) degeneration is the major cause of lower back pain, while the currently available treatments are symptomatic rather than curative. Tissue engineering is a powerful therapeutic strategy that can restore the normal biomechanical motion of the human spine. The ability of a biphasic elastic scaff...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.10.031
更新日期：2008-02-01 00:00:00
abstract：:Particle-associated periprosthetic osteolysis remains a major issue in joint replacement. Ongoing bone loss resulting from wear particle-induced inflammation is accompanied by continued attempts at bone repair. Previously we showed that mesenchymal stem cells (MSCs) are recruited systemically to bone exposed to contin...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:Sub-micron particles of bioactive glass (SMBGs) with composition 85 mol% SiO(2) and 15 mol% CaO were synthesised and characterised. Bioactivity was demonstrated by the formation of calcium apatite following 5 days immersion in simulated body fluid (SBF). The effect of a 24 h exposure of SMBGs (100 μg/ml, 150 μg/ml, 20...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.08.082
更新日期：2011-02-01 00:00:00
abstract：:Focal infections that are caused by antibiotic-resistant bacteria are becoming an ever-growing challenge to human health. To address this challenge, a pH-responsive amphiphilic polymer of polyaniline-conjugated glycol chitosan (PANI-GCS) that can self-assemble into nanoparticles (NPs) in situ is developed. The PANI-GC...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.11.045
更新日期：2017-02-01 00:00:00
abstract：:Due to the architecture of solid body tissues including bone, three-dimensional (3D) in vitro microenvironments appear favorable, since herein cell growth proceeds under more physiological conditions compared to conventional 2D systems. In the present study we show that a 3D microenvironment comprising a fibronectin-c...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.08.023
更新日期：2011-12-01 00:00:00
