Abstract:
:An in vitro cell-implant mineralization model system was used to study the effect of transforming growth factor-beta 1(TGF-beta1) on mineralization in human osteoblast cultures. SaOs-2 and primary human osteoblast (HOB) cells were cultured on Tivanium (Ti-6Al-4V) disks. Administration of different concentrations of TGF-beta1 (0.02, 0.01, 0.2, 1.0, 2.0ng/ml) to these cultures demonstrated a biphasic dose response with 0.2ng/ml TGF-beta1 maximally increasing the calcium content compared to control culture. Results with SaOs-2 and HOB cultures were similar. An optimal dose of TGF-beta1 (0.2ng/ml) was provided to the cultures either in one single dose or multiple doses. Continuous administration of 0.2ng/ml TGF-beta1 caused 77% (SaOs-2) and 60% (HOB) increases in calcification compared to the control and 0.2ng/ml single dose groups. Single administration of the accumulative dose at 1.6ng/ml had no significant effect on the calcium content in either cell culture compared to control. Two weeks continuous administration of 0.2ng/ml TGF-beta1 in both cell cultures resulted in significant increases in the expression of bone specific extracellular matrix proteins which included alkaline phosphatase, Type I collagen, and osteocalcin as measured by Northern blot analysis and RT-PCR. At 4 weeks, the mRNA level of Type I collagen was still significantly higher in the TGF-beta1 treatment group compared to control. In conclusion, TGF-beta1 enhances mineralization of HOB on implant materials.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Zhang H,Ahmad M,Gronowicz Gdoi
10.1016/s0142-9612(02)00616-6keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
2013-20issue
12eissn
0142-9612issn
1878-5905pii
S0142961202006166journal_volume
24pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::Non-covalent interactions are often regarded as insufficient to construct macroscopic materials of substantial integrity and cohesion. However, the low binding energy of such reversible interactions can be compensated by increasing their number to work in concert to create strong materials. Here we present the success...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.05.003
更新日期:2014-08-01 00:00:00
abstract::Meniscus injuries are prevalent in orthopedic diagnosis. The reconstruction of the structural inhomogeneity and anisotropy of the meniscus is a major challenge in clinical practice. Meniscal tissue engineering has emerged as a potential alternative for the treatment of various meniscal diseases and injuries. In this s...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120466
更新日期:2021-01-01 00:00:00
abstract::Periosteal derived bovine osteoblast-like cells migrated in culture onto an ionomeric cement. Cell cultures were maintained for 4 weeks and used to study the in vitro behaviour of cells on the ionomeric bone cement (IC). The cells produced bone matrix proteins (osteocalcin, bone sialoprotein II) and were osteoblast-li...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(93)90133-m
更新日期:1993-10-01 00:00:00
abstract::Biodegradable and pH-sensitive hydrogels containing azoaromatic moieties were synthesized from the same polymeric precursors by two synthetic methods, namely a polymer-polymer reaction and cross-linking of polymeric precursors. The effect of the synthetic route employed and the detailed network structure on in vitro d...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(97)00007-0
更新日期:1997-06-01 00:00:00
abstract::The influence of crystallization, upon heat treatment, on the properties of high-velocity oxy-fuel (HVOF) sprayed hydroxyapatite (HA) coatings was investigated. The characterization of the HA coating was performed by X-ray diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Differential Scanning Calo...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00326-x
更新日期:2002-05-01 00:00:00
abstract::Poly(ω-pentadecalactone-co-p-dioxanone) [poly(PDL-co-DO)] copolyesters are copolymers of an isodimorphic system, which remain semicrystalline over the whole range of compositions. Here, we evaluated enzymatically synthesized poly(PDL-co-DO) copolymers as new materials for biomedical applications. In vivo experiments u...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.05.046
更新日期:2011-09-01 00:00:00
abstract::Saturated neoglycopolymers, prepared via tandem ROMP-hydrogenation (ROMP=ring-opening metathesis polymerization) of carbohydrate-functionalized norbornenes, are investigated as novel collagen crosslinking agents in corneal tissue engineering. The neoglycopolymers were incorporated into recombinant human collagen type ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.06.016
更新日期:2009-10-01 00:00:00
abstract::Skeletal muscle is a highly organized tissue in which the extracellular matrix (ECM) is composed of highly-aligned cables of collagen with nanoscale feature sizes, and provides structural and functional support to muscle fibers. As such, the transplantation of disorganized tissues or the direct injection of cells into...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.10.067
更新日期:2014-02-01 00:00:00
abstract::Bone defects, such as compressive fractures in the vertebral bodies, are frequently treated with acrylic bone cements (e.g., PMMA). Although these biomaterials have sufficient mechanical properties for fixing the fracture, they are non-degradable and do not remodel or integrate with host tissue. In an alternative appr...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.12.046
更新日期:2008-04-01 00:00:00
abstract::Films of polyoxyethylene network were prepared from two types of triethoxysilane-terminated prepolymers. In this way, films of polyoxyethylene network with possible applications in the biomedical field could be made easier. To test their biocompatibility, these networks were added to organ cultures of adult human skin...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(92)90155-h
更新日期:1992-01-01 00:00:00
abstract::Polylactide (PLA) microspheres were coated with collagen for cell culture and injectable cell carriers. Utilizing a method of emulsion-solvent evaporation, PLA microspheres with diameter ranging from 180 to 280 microm were prepared, followed with aminolysis in hexanediamine/n-propanol solution to introduce free amino ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.03.038
更新日期:2005-11-01 00:00:00
abstract::DNA vaccine is a milestone in contemporary vaccine development. It has considerably offset many shortcomings in conventional vaccines. Although DNA vaccines applied through 'traditional' high-pressure gene guns generally elicit high titers of protective immunity, such a practice however requires enormous investment in...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.07.029
更新日期:2009-10-01 00:00:00
abstract::Carbon fiber implants were used to fill osteochondral defects created on the articular surface of the patella of 36 rabbits, for the purpose of studying the long-term histological changes of the repair process. Six months after surgery the defect was filled by fibrous tissue, where the superficial area was organized p...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00144-7
更新日期:2000-11-01 00:00:00
abstract::Leveraging the multifunctional nature of citrate in chemistry and inspired by its important role in biological tissues, a class of highly versatile and functional citrate-based materials (CBBs) has been developed via facile and cost-effective polycondensation. CBBs exhibiting tunable mechanical properties and degradat...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2018.05.003
更新日期:2018-09-01 00:00:00
abstract::Electrically conducting polymers hold promise for developing advanced neuroprostheses, bionic systems and neural repair devices. Among them, poly(3, 4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS) exhibits superior physicochemical properties but biocompatibility issues have limited its use. We d...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.08.057
更新日期:2010-12-01 00:00:00
abstract::Anti-angiogenic therapeutics has been investigated extensively for the treatment of retinal and choroidal vascular diseases, and diabetic retinopathy. Anti-Flt1 peptide of GNQWFI is an antagonistic peptide for vascular endothelial growth factor receptor 1 (VEGFR1 or Flt1) inhibiting VEGFR1-mediated endothelial cell mi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.01.003
更新日期:2011-04-01 00:00:00
abstract::Cytokines, chemokines, and growth factors were analyzed periodically over eight weeks from the wound exudate fluid surrounding biomaterials implanted subcutaneously within stainless steel mesh cages. TNF-alpha, MCP-1, MIP-1alpha, IL-2, IL-6, IL-1beta, VEGF, IL-4, and IL-10 were measured from exudate samples collected ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.09.026
更新日期:2009-01-01 00:00:00
abstract::Tissue engineering of the cornea could overcome shortages of donor corneas for transplantation and improve quality. Our aim was to grow an endothelial layer on a substratum suitable for transplant. Silkworm (Bombyx mori) fibroin was prepared as 5 μm thick transparent membranes. The B4G12 cell line was used to assess a...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.12.034
更新日期:2011-06-01 00:00:00
abstract::Integrins represent the primary mechanism of cell-extracellular matrix interactions and control cell morphology, proliferation, and differentiation. We have previously shown that substrate-dependent modulation of adsorbed fibronectin (Fn) conformation alters alpha5beta1 integrin binding to Fn and directs C2C12 myoblas...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00387-8
更新日期:2002-06-01 00:00:00
abstract::When subjected to a monotonically increasing deformation state, the mechanical behavior of UHMWPE is characterized by a linear elastic response followed by distributed yielding and strain hardening at large deformations. During the unloading phases of an applied cyclic deformation process, the response is characterize...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00367-2
更新日期:2002-06-01 00:00:00
abstract::Three proprietary dental resins were found to include acrylates (as distinct from methacrylates). As acrylates are well known to have adverse skin reactions, the nature and content of the acrylates were obtained. One resin contained methyl acrylate, and the other two ethyl acrylate. Also, the extractable free monomer ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90047-e
更新日期:1991-08-01 00:00:00
abstract::The construction of tissue-engineered devices for medical applications is now possible in vitro using cell culture and bioreactors. Although methods of incorporating them back into the host are available, current constructs depend purely on diffusion which limits their potential. The absence of a vascular network capa...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2004.07.006
更新日期:2005-05-01 00:00:00
abstract::Lung fibrosis is a poor prognostic factor for pulmonary adenocarcinoma, and the effect of a rigid microenvironment on cancer behavior is unclear. We cultured A549 cells on matrices of 0.2, 2, and 25 kPa to mimic the rigidities of normal lung parenchyma, progressive fibrotic change, and lung fibrosis, respectively. Lun...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.04.058
更新日期:2015-08-01 00:00:00
abstract::Metals and ceramics are commonly used in orthopaedics, dentistry and other load bearing applications. However, the use of ceramic matrix composites reinforced with biocompatible metals for heavy load-bearing hard tissue replacement applications has not previously been reported. In order to improve the reliability and ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.058
更新日期:2016-01-01 00:00:00
abstract::Virus-based nanoparticles have shown promise as vehicles for delivering therapeutic genes. However, the rational design of viral vectors that enable selective tropism towards particular types of cells and tissues remains challenging. Here, we explored structural-functional relationships of the adeno-associated virus 2...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.11.066
更新日期:2016-02-01 00:00:00
abstract::Recapitulation of the cellular microenvironment of the heart, which promotes cell contraction, remains a key challenge in cardiac tissue engineering. We report here on our work, where for the first time, a 3-dimensional (3D) spring-like fiber scaffold was fabricated, successfully mimicking the coiled perimysial fibers...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.07.054
更新日期:2013-11-01 00:00:00
abstract::Bioengineered tissue equivalents should provide physiologically relevant biochemical and mechanical cues to support the growth and differentiation of seeded cells. Herein, tubular dense collagen constructs (TDCCs) with collagen content comparable to native extracellular matrix were used to investigate the effect of sh...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.11.025
更新日期:2013-03-01 00:00:00
abstract::The hydrophilic nature of hydrogel matrices makes them disadvantageous to entrap poorly soluble therapeutic agents and greatly restricts their applications as drug-delivery systems. In this study, we demonstrated that sustained delivery of lipophilic drugs in hydrogel-based devices can be readily achieved by enhancing...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.12.047
更新日期:2009-04-01 00:00:00
abstract::To explore molecular mechanism of mediation of adsorbed proteins to cell adhesion and growth on biomaterials, this study examined endothelial cell adhesion, morphology and viability on bare and titanium nitride (TiN) coated nickel titanium (NiTi) alloys and chitosan film firstly, and then identified the type and amoun...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.04.028
更新日期:2013-07-01 00:00:00
abstract::Multilayer nanofilms, formed by the layer-by-layer (LbL) adsorption of positively and negatively charged polyelectrolytes, are promising substrates for tissue engineering. We investigate here the attachment and function of hepatic cells on multilayer films in terms of film composition, terminal layer, rigidity, charge...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.06.027
更新日期:2008-10-01 00:00:00