Abstract:
:Recapitulation of the cellular microenvironment of the heart, which promotes cell contraction, remains a key challenge in cardiac tissue engineering. We report here on our work, where for the first time, a 3-dimensional (3D) spring-like fiber scaffold was fabricated, successfully mimicking the coiled perimysial fibers of the heart. We hypothesized that since in vivo straightening and re-coiling of these fibers allow stretching and contraction of the myocardium in the direction of the cardiomyocytes, such a scaffold can support the assembly of a functional cardiac tissue capable of generating a strong contraction force. In this study, the mechanical properties of both spring-like single fibers and 3D scaffolds composed of them were investigated. The measurements showed that they have increased elasticity and extensibility compared to corresponding straight fibers and straight fiber scaffolds. We have also shown that cardiac cells cultivated on single spring-like fibers formed cell-fiber interactions that induced fiber stretching in the direction of contraction. Moreover, cardiac cells engineered within 3D thick spring-like fiber scaffolds formed a functional tissue exhibiting significantly improved function, including stronger contraction force (p = 0.002), higher beating rate (p < 0.0001) and lower excitation threshold (p = 0.02), compared to straight fiber scaffolds. Collectively, our results suggest that spring-like fibers can play a key role in contributing to the ex vivo formation of a contracting cardiac muscle tissue. We envision that cardiac tissues engineered within these spring-like fiber scaffolds can be used to improve heart function after infarction.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Fleischer S,Feiner R,Shapira A,Ji J,Sui X,Daniel Wagner H,Dvir Tdoi
10.1016/j.biomaterials.2013.07.054subject
Has Abstractpub_date
2013-11-01 00:00:00pages
8599-606issue
34eissn
0142-9612issn
1878-5905pii
S0142-9612(13)00852-1journal_volume
34pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::This study is concerned with the effect of dual implantation of calcium and phosphorus upon the structure, corrosion resistance and biocompatibility of titanium. The ions were implanted in sequence, first Ca and then P, both at a dose of 10(17) ions/cm2 at a beam energy of 25 keV. Transmission electron microscopy was ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.08.015
更新日期:2005-06-01 00:00:00
abstract::A human-human oligodendroglial cell line MO3.13 was chosen in this study to model the loss of oligodendrocytes that occurs during episodes of multiple sclerosis. The influence of mercuric chloride (HgCl(2)) upon cell viability specifically the mode of cell death, whether by an active apoptotic mechanism or passive nec...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00436-2
更新日期:2003-03-01 00:00:00
abstract::The design of peptide-based subunit vaccine formulations for the direct delivery of tumor antigen peptides (Aps) to dendritic cells (DCs) localized within draining lymph nodes (DLNs) is challenging. Mature DCs (mDCs) are abundantly distributed within DLNs but have dramatically reduced endocytic uptake and antigen-proc...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.05.008
更新日期:2016-08-01 00:00:00
abstract::We report here the study of the biocompatibility of a bone graft material, the Pyrost, using a previously established in vitro model of human osteoblasts. The effect of this material on cell proliferation was evaluated by the MTS assay. Results indicated the absolute absence of cytotoxic or cytostatic effect of Pyrost...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(98)00090-8
更新日期:1998-10-01 00:00:00
abstract::The magnetic resonance (MR) functionalities of pullulan-conjugated gadolinium diethylene triamine pentaacetate (Gd-DTPA-Pullulan) as a new hepatocyte-specific contrast agent were evaluated. Pullulan, which specifically accumulates on hepatocytes via asialoglycoprotein receptors, was chemically linked with Gd-DTPA. Gd-...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.03.069
更新日期:2011-08-01 00:00:00
abstract::Metastasis is the major cause of high mortality in head and neck squamous cell carcinoma (HNSCC), in which HNSCC-derived cancer stem cells (CSCs) may be involved. Several reports have coupled non-viral gene delivery with RNA interference (RNAi) to target specific genes in cancer cells. However, the delivery efficiency...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.01.016
更新日期:2012-05-01 00:00:00
abstract::At high concentrations, manganese (Mn) promotes cellular neurodevelopment but causes toxicity. Here, we report that Mn ion at high concentrations can be delivered to pheochromocytoma 12 (PC12) cells using gold nanoparticles (AuNPs) to enhance cellular neurodevelopment without toxicity. Mn(2+) release from AuNPs was de...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.03.025
更新日期:2015-07-01 00:00:00
abstract::We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.07.066
更新日期:2010-11-01 00:00:00
abstract::New light-activation units equipped with high-illuminant blue light-emitting diodes (LEDs) have recently been proposed as a replacement for the halogen units that are widely used in dentistry to polymerize light-cured resins. The photoinitiators in light-cured dental resins, typified by the camphorquinone (CQ)/amine p...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00636-1
更新日期:2003-05-01 00:00:00
abstract::This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.05.082
更新日期:2011-07-01 00:00:00
abstract::Lesions in the avascular part of 20 canine menisci were repaired by implantation of a porous polyurethane. Seven menisci were not repaired and served as controls. The repair tissue was characterized by biochemical and immunological analysis. The role of vascularity in healing was studied by perfusion of menisci with I...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(96)87648-4
更新日期:1996-09-01 00:00:00
abstract::Bioglass 45S5 is a silica-based melt-derived glass, used in medical field as a bone regenerative material because of the deposition of a layer of hydroxy carbonate apatite (HCA) on the surface of the glass when immersed in body fluid. The present paper studies the early steps of reaction of 2-microm sized particles of...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.07.009
更新日期:2005-05-01 00:00:00
abstract::A new mathematical model was developed and an exact analytical solution without approximations of previous work was derived for the description of the kinetics and equilibrium characteristics of drug loading from a finite external solution onto ion-exchange microspheres. The influence of important parameters pertinent...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.02.011
更新日期:2006-07-01 00:00:00
abstract::Hydrophobically derivatized hyperbranched polyglycerol (HPG)-polyethylene glycol (PEG) polymers bearing stearoyl chains (HPG-C18-PEG) were originally developed as human serum albumin substitutes and further as a unimolecular drug delivery system. In view of these in vivo applications and the potential for membrane int...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.01.021
更新日期:2010-04-01 00:00:00
abstract::Efficient and site-specific delivery of anticancer drugs to tumors is important in the development of effective cancer chemotherapy. As an undecapeptide of the tachykinin neuropeptide family, the substance P (SP)/neurokinin-1 receptor (NK1R) system has been identified as a promising ligand-receptor pair in tumor-speci...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.11.011
更新日期:2017-03-01 00:00:00
abstract::In order to increase the functionality of islets encapsulated in a biohybrid artificial pancreas (BAP), it was proposed that co-encapsulation with insulinotropic agents would improve insulin secretion from islets. To prevent agents from leaking out, conjugation with high-molecular-weight polymers was inevitable. In th...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2004.09.045
更新日期:2005-06-01 00:00:00
abstract::The formation of bone tissue in pores, and around, a block made of partially dehydroxylated porous hydroxyapatite ceramic and implanted into a rat's femur using X-ray diffraction (XRD), infrared spectroscopy (IRS) and histological methods were investigated. Structural characteristics and composition of new bone tissue...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(98)00024-6
更新日期:1998-07-01 00:00:00
abstract::Composite particles made by growing nanoscopic silicon wires from the surface of monodispersed, microsized silica beads were tested in this study for their ability to affect the integrity and permeability of an epithelial cell layer. Polyethylene glycol (PEG) is known to sterically stabilize particles and prevent prot...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.11.010
更新日期:2012-02-01 00:00:00
abstract::To prevent bone metastasis, we developed polyethylene glycol (PEG)-conjugated aspartic acid (Asp)-modified liposomes (PEG-Asp-Lipo) as a bone-targeting carrier of paclitaxel (PTX) by using Asp-modified 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-Asp). The affinity of Asp-modified liposomes to hydroxyapatite...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.10.053
更新日期:2018-02-01 00:00:00
abstract::One of the major challenges in engineering thick, complex tissues such as cardiac muscle, is the need to pre-vascularize the engineered tissue in vitro to enable its efficient integration with host tissue upon implantation. Herein, we explored new magnetic alginate composite scaffolds to provide means of physical stim...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.02.037
更新日期:2012-06-01 00:00:00
abstract::Cells form hollow, spheroidal structures during the development of many tissues, including the ocular lens, inner ear, and many glands. Therefore, techniques for in vitro formation of hollow spheroids are valued for studying developmental and disease processes. Current in vitro methods require cells to self-organize i...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.07.031
更新日期:2017-10-01 00:00:00
abstract::Peripheral nerve injuries often lead to incomplete recovery and contribute to significant disability to approximately 360,000 people in the USA each year. Stem cell therapy holds significant promise for peripheral nerve regeneration, but maintenance of stem cell viability and differentiation potential in vivo are stil...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2018.07.015
更新日期:2018-10-01 00:00:00
abstract::To fully understand biological behavior in vitro often dictates that oxygen be reported at either a local or a cellular level. Oxygen sensors based on the luminescent quenching of a specific form of electrospun fiber were developed for measurement of both gaseous and dissolved oxygen concentrations. Electrospinning wa...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.055
更新日期:2016-01-01 00:00:00
abstract::Breast cancer cells (BCC) and macrophages are known to interact via epidermal growth factor (EGF) produced by macrophages and colony stimulating factor-1 (CSF-1) produced by BCC. Despite contradictory findings, this interaction is perceived as a paracrine loop. Further, the underlying mechanism of interaction remains ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120412
更新日期:2021-01-01 00:00:00
abstract::Bone marrow is the primary hematopoietic organ, which is involved in multiple malignant diseases including acute and chronic leukemia, multiple myeloma, myelodysplastic syndromes, and bone metastases from solid tumors. These malignancies affect normal homeostasis and reshape the bone marrow microenvironment. There are...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2017.11.029
更新日期:2018-02-01 00:00:00
abstract::Polyvinyl alcohol (PVA) cylindrical hydrogel with a stiffness gradient was prepared using a simple liquid nitrogen (LN2)-contacting gradual freezing and thawing method in order to investigate the effects of substrate stiffness on stem cell differentiation into specific cell types. The prepared cylindrical PVA hydrogel...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.11.017
更新日期:2015-02-01 00:00:00
abstract::Medical implants used in oral and orthopaedic surgery are mainly produced from titanium. Their biological behaviour, e.g. osseointegration, essentially depends on both the chemical composition and the morphology of the surface. Modifications achieved by excimer laser irradiation of titanium samples were investigated i...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00318-1
更新日期:2003-10-01 00:00:00
abstract::Metal sensitivity (MS) reactions to implant metals represent a rare but well-documented complication following total joint arthroplasty (TJA). Although 20-25% of post-TJA patients develop MS, only a few highly susceptible patients (< 1%) exhibit symptoms. Whether surgeons should perform screening for MS is currently a...
journal_title:Biomaterials
pub_type: 临床试验,杂志文章
doi:10.1016/j.biomaterials.2004.03.038
更新日期:2005-03-01 00:00:00
abstract::Modeling the interaction between the supportive stroma and the hematopoietic stem and progenitor cells (HSPC) is of high interest in the regeneration of the bone marrow niche in blood disorders. In this work, we present an injectable co-culture system to study this interaction in a coherent in vitro culture and in viv...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.119665
更新日期:2020-02-01 00:00:00
abstract::Hypoxia, as an inevitable characteristic of solid tumors, has been regarded as a noticeably causative factor to therapeutic resistance and metastatic variants. Exploring novel theranostics to realize the accurate diagnosis of hypoxia and the simultaneous implementation of effective therapy is a promising prospect for ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2018.05.018
更新日期:2018-08-01 00:00:00