Cancer cell aggregate hypoxia visualized in vitro via biocompatible fiber sensors.


:To fully understand biological behavior in vitro often dictates that oxygen be reported at either a local or a cellular level. Oxygen sensors based on the luminescent quenching of a specific form of electrospun fiber were developed for measurement of both gaseous and dissolved oxygen concentrations. Electrospinning was used to fabricate "core-shell" fiber configurations in which oxygen-sensitive transition-metal porphyrin complexes are embedded in an optically clear, gas permeable polycarbonate polymer 'core' while polycaprolactone provided a protective yet biocompatible 'shell'. By taking advantage of the resulting high sensitivity and fast response of electrospun core-shell fiber sensors, we were able to locate and image hypoxic regions in contact with aggregates of glioblastoma cells. Nanoscale, biomimetic sensors containing oxygen-sensitive porphyrins are particularly well suited to biological applications. These 'smart' nanofiber based sensors do not consume oxygen, their mechanical and chemical characteristics can be finely tuned allowing tailoring of biocompatibility and microstructure. Core-shell nanofiber oxygen sensing fibers could provide real-time assessments of tumor cell response to pharmacological innovations designed to target hypoxic regions driving new knowledge and technological advancement.






Xue R,Nelson MT,Teixeira SA,Viapiano MS,Lannutti JJ




Has Abstract


2016-01-01 00:00:00












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    pub_type: 杂志文章


    authors: Xu R,Huang L,Wei W,Chen X,Zhang X,Zhang X

    更新日期:2016-07-01 00:00:00

  • Physical-mechanical, moisture absorption and bioadhesive properties of hydroxypropylcellulose hot-melt extruded films.

    abstract::The objective of this study was to investigate the moisture absorption, physical-mechanical and bioadhesive properties of hot-melt extruded hydroxypropylcellulose (HPC) films containing polymer additives. These additives included polyethylene glycol (PEG) 5%, polycarbophil 5%, carbomer 5%, Eudragit E-100 5%, and sodiu...


    pub_type: 杂志文章


    authors: Repka MA,McGinity JW

    更新日期:2000-07-01 00:00:00

  • Kinetics of oxygen uptake by cells potentially used in a tissue engineered trachea.

    abstract::Synthetic polymer scaffold seeded with autologous cells have a clinical translational potential. A rational design oriented to clinical applications must ensure an efficient mass transfer of nutrients as a function of specific metabolic rates, especially for precariously vascularized tissues grown in vitro or integrat...


    pub_type: 杂志文章


    authors: Curcio E,Piscioneri A,Morelli S,Salerno S,Macchiarini P,De Bartolo L

    更新日期:2014-08-01 00:00:00

  • Isothermal microcalorimetry: an analytical technique for assessing the dynamic chemical stability of UHMWPE.

    abstract::In this work, isothermal microcalorimetry (IMC) was utilized to measure the exothermic heat flow from specimens of ultra-high-molecular-weight polyethylene (UHMWPE), that had been sterilized by various standard methods, under simulated shelf storage (air at 25 degrees C, 30% r.h.) and simulated implantation (phosphate...


    pub_type: 杂志文章


    authors: Charlebois SJ,Daniels AU,Lewis G

    更新日期:2003-01-01 00:00:00

  • Tailoring polymeric hybrid micelles with lymph node targeting ability to improve the potency of cancer vaccines.

    abstract::It has been widely accepted that lymph nodes (LNs) are critical targets of cancer vaccines and particles sized between 10 and 100 nm with a neutral or negative surface charge are preferred for lymphatic transfer after subcutaneous or intradermal injection. However their limited uptake by antigen presenting cells (APCs...


    pub_type: 杂志文章


    authors: Zeng Q,Li H,Jiang H,Yu J,Wang Y,Ke H,Gong T,Zhang Z,Sun X

    更新日期:2017-04-01 00:00:00

  • Comparison of polymerically stabilized PEG-grafted liposomes and physically adsorbed carboxymethylchitin and carboxymethyl/glycolchitin liposomes for biological applications.

    abstract::The stabilities of two types of polymerically stabilized liposomes consisting of PEG-grafted (DSPC:CHOL:DSPE-PEG1900, 5:4:1) and physically adsorbed carboxymethylchitin (CMC) and carboxymethyl/glycolchitin (CO) are compared. The polyelectrolyte is adsorbed on positive (DSPC:CHOL:DMTAP, 5:4:1) and neutral (DSPC:CHOL, 1...


    pub_type: 杂志文章


    authors: Mobed M,Chang TM

    更新日期:1998-07-01 00:00:00

  • Sequential delivery of immunomodulatory cytokines to facilitate the M1-to-M2 transition of macrophages and enhance vascularization of bone scaffolds.

    abstract::In normal tissue repair, macrophages exhibit a pro-inflammatory phenotype (M1) at early stages and a pro-healing phenotype (M2) at later stages. We have previously shown that M1 macrophages initiate angiogenesis while M2 macrophages promote vessel maturation. Therefore, we reasoned that scaffolds that promote sequenti...


    pub_type: 杂志文章


    authors: Spiller KL,Nassiri S,Witherel CE,Anfang RR,Ng J,Nakazawa KR,Yu T,Vunjak-Novakovic G

    更新日期:2015-01-01 00:00:00

  • In vivo distribution, pharmacokinetics, and toxicity of aqueous synthesized cadmium-containing quantum dots.

    abstract::Fluorescent Ⅱ-Ⅳ Quantum dots (QDs) have demonstrated to be highly promising biological probes for various biological and biomedical applications due to their many attractive merits, such as robust photostabilty, strong photoluminescence, and size-tunable fluorescence. Along with wide ranging bioapplications, concerns ...


    pub_type: 杂志文章


    authors: Su Y,Peng F,Jiang Z,Zhong Y,Lu Y,Jiang X,Huang Q,Fan C,Lee ST,He Y

    更新日期:2011-09-01 00:00:00

  • Effects of synthetic micro- and nano-structured surfaces on cell behavior.

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    pub_type: 杂志文章,评审


    authors: Flemming RG,Murphy CJ,Abrams GA,Goodman SL,Nealey PF

    更新日期:1999-03-01 00:00:00

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    abstract::Suture materials may interact with immune competent cells and thereby affect localized immunity. Macrophages are central to the inflammatory response and coordinate wound healing. They are also involved in the clearance of foreign material, bacteria and malignant cells. We studied the influence of soluble factors asso...


    pub_type: 杂志文章


    authors: Uff CR,Scott AD,Pockley AG,Phillips RK

    更新日期:1995-03-01 00:00:00

  • Experimental evaluation of a resorbable intramedullary plug for cemented total hip replacement.

    abstract::In order to evaluate degradation kinetics and biocompatibility of a resorbable poly(D,L-lactic acid) (PDLLA) plug for total cemented hip prostheses, an experimental in vitro and in vivo study was carried out. Degradation rate studies were performed in Ringer solution and after in vivo plug implantation in the femoral ...


    pub_type: 杂志文章


    authors: Giardino R,Fini M,Aldini NN,Giavaresi G,Rocca M,Orienti L,de Lollis A,Fambri L

    更新日期:1997-07-01 00:00:00

  • A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis.

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    authors: Yang G,Fan M,Zhu J,Ling C,Wu L,Zhang X,Zhang M,Li J,Yao Q,Gu Z,Cai X

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    pub_type: 杂志文章


    authors: Rampoldi A,Jha R,Fite J,Boland G,Xu C

    更新日期:2021-01-11 00:00:00

  • Crystal structure characterization of nautilus shell at different length scales.

    abstract::In this work, we studied the shell structure of the Nautilus pompilius Linnaeus by using analytical techniques of scanning (SEM) and transmission electron microscopy (TEM) and X-ray diffraction. The main objective of this study is the structural characterization of Nautilus shell at different length levels, from micro...


    pub_type: 杂志文章


    authors: Velázquez-Castillo RR,Reyes-Gasga J,García-Gutierrez DI,Jose-Yacaman M

    更新日期:2006-09-01 00:00:00

  • The effect of squalane-dissolved fullerene-C60 on adipogenesis-accompanied oxidative stress and macrophage activation in a preadipocyte-monocyte co-culture system.

    abstract::Effects of squalane-dissolved fullerene-C60 (Sql-fullerene) on macrophage activation and adipose conversion with oxidative stress were studied using an inflammatory adipose-tissue equivalent (ATE) and OP9 mouse stromal preadipocyte-U937 lymphoma cell co-culture systems. Differentiation of OP9 cells was initiated by in...


    pub_type: 杂志文章


    authors: Xiao L,Aoshima H,Saitoh Y,Miwa N

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    pub_type: 杂志文章


    authors: Tan GK,Dinnes DL,Butler LN,Cooper-White JJ

    更新日期:2010-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Cook WD,Liem F,Russo P,Scheiner M,Simkiss G,Woodruff P

    更新日期:1984-09-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Curcio E,Salerno S,Barbieri G,De Bartolo L,Drioli E,Bader A

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    abstract::Parkinson's disease (PD) is an oxidative stress-mediated neurodegenerative disorder caused by selective dopaminergic neuronal death in the midbrain substantia nigra. Paraoxonase 1 (PON1) is a potent inhibitor of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) against oxidation by destroying biological...


    pub_type: 杂志文章


    authors: Kim MJ,Park M,Kim DW,Shin MJ,Son O,Jo HS,Yeo HJ,Cho SB,Park JH,Lee CH,Kim DS,Kwon OS,Kim J,Han KH,Park J,Eum WS,Choi SY

    更新日期:2015-09-01 00:00:00

  • Effect of different Ti-6Al-4V surface treatments on osteoblasts behaviour.

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    pub_type: 杂志文章


    authors: Ku CH,Pioletti DP,Browne M,Gregson PJ

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Kiang T,Wen J,Lim HW,Leong KW

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  • Vascularized neural constructs for ex-vivo reconstitution of blood-brain barrier function.

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    pub_type: 杂志文章


    authors: Yue H,Xie K,Ji X,Xu B,Wang C,Shi P

    更新日期:2020-07-01 00:00:00

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    pub_type: 杂志文章


    authors: Richard-Fiardo P,Hervouet C,Marsault R,Franken PR,Cambien B,Guglielmi J,Warnez-Soulie J,Darcourt J,Pourcher T,Colombani T,Haudebourg T,Peuziat P,Pitard B,Vassaux G

    更新日期:2015-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Blacklock J,Vetter A,Lankenau A,Oupický D,Möhwald H

    更新日期:2010-09-01 00:00:00

  • A microchip filter device incorporating slit arrays and 3-D flow for detection of circulating tumor cells using CAV1-EpCAM conjugated microbeads.

    abstract::Circulating tumor cells (CTCs) are rare cells and the presence of these cells may indicate a poor prognosis and a high potential for metastasis. Despite highly promising clinical applications, CTCs have not been investigated thoroughly, due to many technical limitations faced in their isolation and identification. Cur...


    pub_type: 杂志文章


    authors: Kim YJ,Koo GB,Lee JY,Moon HS,Kim DG,Lee DG,Lee JY,Oh JH,Park JM,Kim MS,Woo HG,Kim SI,Kang P,Choi W,Sim TS,Park WY,Lee JG,Kim YS

    更新日期:2014-08-01 00:00:00

  • 2-Methacryloyloxyethyl phosphorylcholine polymer (MPC)-coating improves the transfection activity of GALA-modified lipid nanoparticles by assisting the cellular uptake and intracellular dissociation of plasmid DNA in primary hepatocytes.

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    pub_type: 杂志文章


    authors: Ukawa M,Akita H,Masuda T,Hayashi Y,Konno T,Ishihara K,Harashima H

    更新日期:2010-08-01 00:00:00

  • Regulation of metastatic ability and drug resistance in pulmonary adenocarcinoma by matrix rigidity via activating c-Met and EGFR.

    abstract::Lung fibrosis is a poor prognostic factor for pulmonary adenocarcinoma, and the effect of a rigid microenvironment on cancer behavior is unclear. We cultured A549 cells on matrices of 0.2, 2, and 25 kPa to mimic the rigidities of normal lung parenchyma, progressive fibrotic change, and lung fibrosis, respectively. Lun...


    pub_type: 杂志文章


    authors: Chang CC,Hsieh TL,Tiong TY,Hsiao CH,Ji AT,Hsu WT,Lee OK,Ho JH

    更新日期:2015-08-01 00:00:00

  • A preliminary report on the biocompatibility of photopolymerizable semi-interpenetrating anhydride networks.

    abstract::A new family of poly(anhydrides) (PA) has been developed which can be cured photochemically to produce degradable networks. These degradable anhydride networks may be useful in orthopaedics as bone cements and as matrices for drug delivery. This system, which is a semi-interpenetrating network (semi-IPN), has been eva...


    pub_type: 杂志文章


    authors: Shastri VP,Padera RF,Tarcha P,Langer R

    更新日期:2004-02-01 00:00:00