Abstract:
:Bioengineered tissue equivalents should provide physiologically relevant biochemical and mechanical cues to support the growth and differentiation of seeded cells. Herein, tubular dense collagen constructs (TDCCs) with collagen content comparable to native extracellular matrix were used to investigate the effect of shear stress alone (i.e. under laminar fluid flow), and shear stress in combination with circumferential strain (i.e. under pulsatile fluid flow) on the proliferation, alignment, and phenotype of three-dimensionally (3D) seeded airway smooth muscle cells (ASMCs). In addition, the effect of ASMC-mediated remodelling on TDCC matrix morphological and mechanical properties was investigated. Compared to static culture, pulsatile flow increased seeded ASMC growth by 70%, improved the homogeneity of cell distribution within the TDCCs and induced differential cellular alignment depending on the primary stimuli. Specifically, within the inner wall, where shear stress is predominant, ASMCs were aligned parallel to fluid flow direction, while within the outer wall ASMCs were aligned parallel to the circumferential strain (perpendicular to fluid flow). In contrast, under laminar flow, ASMCs were aligned parallel to fluid flow direction within both walls. Compared to laminar flow, pulsatile flow resulted in increased positive staining for α-smooth muscle actin, and in up-regulated typical ASMC contractile markers suggesting that circumferential strain modulates ASMC differentiation. Pulsatile flow also caused a 60 and 30% increase in collagen density within both acellular and cellular TDCCs, respectively, which was reflected in an increased apparent modulus. Compared to static culture, pulsatile stimulation of cellular constructs resulted in 70% higher circumferential strength. The TDCCs provide ASMC niche for greater insight into the responses of 3D seeded SMCs to physiologically equivalent in vitro dynamic conditioning.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Ghezzi CE,Risse PA,Marelli B,Muja N,Barralet JE,Martin JG,Nazhat SNdoi
10.1016/j.biomaterials.2012.11.025subject
Has Abstractpub_date
2013-03-01 00:00:00pages
1954-66issue
8eissn
0142-9612issn
1878-5905pii
S0142-9612(12)01269-0journal_volume
34pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::A growing number of protein-based fibrous biomaterials have been produced with a cross-β amyloid core yet the long-term effect of these materials on cell viability and the influence of core and non-core protein sequences on viability is not well understood. Here, synthetic bioactive TTR1-RGD and control TTR1-RAD or TT...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.12.039
更新日期:2015-04-01 00:00:00
abstract::The use of non-viral nanoparticulate systems for the delivery of therapeutic agents is receiving considerable attention for medical and pharmaceutical applications. This increasing interest results from the fact that these systems can be designed to meet specific physicochemical requirements, and they display low toxi...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2005.05.044
更新日期:2005-12-01 00:00:00
abstract::Metal sensitivity (MS) reactions to implant metals represent a rare but well-documented complication following total joint arthroplasty (TJA). Although 20-25% of post-TJA patients develop MS, only a few highly susceptible patients (< 1%) exhibit symptoms. Whether surgeons should perform screening for MS is currently a...
journal_title:Biomaterials
pub_type: 临床试验,杂志文章
doi:10.1016/j.biomaterials.2004.03.038
更新日期:2005-03-01 00:00:00
abstract::The efficient generation of integration- and xeno-free iPSCs is a prerequisite for their use in clinical applications. Furthermore, non-invasiveness of somatic cell acquisition for iPSC generation is another factor to consider. In this study, we established a practical, simple, and convenient method to generate integr...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.05.059
更新日期:2014-09-01 00:00:00
abstract::Multiwalled carbon nanotubes (MWCNTs) are cut short and grafted with polyethylenimine (PEI) for further covalent conjugation to fluorescein isothiocyanate (FITC) and prostate stem cell antigen (PSCA) monoclonal antibody (mAb). The in vitro and in vivo toxicity data reveal that the as-prepared CNT-PEI(FITC)-mAb has goo...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.03.038
更新日期:2014-07-01 00:00:00
abstract::We developed cell-free implants, comprising carbodiimide crosslinked recombinant human collagen (RHC), to enable corneal regeneration by endogenous cell recruitment, to address the worldwide shortage of donor corneas. Patients were grafted with RHC implants. Over four years, the regenerated neo-corneas were stably int...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.11.079
更新日期:2014-03-01 00:00:00
abstract::The metabolic disposition and elimination process of the anhydride co-polymer poly[1,3-bis(p-carboxy-phenoxypropane):sebacic acid] 20:80 [P(CPP:Sa)20:80] implanted in the rat brain was studied. Two polymers were prepared, one with [14C]SA and unlabelled CPP, and the other co-polymer with [14C]CPP and unlabelled SA. Wi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(94)90166-x
更新日期:1994-07-01 00:00:00
abstract::Glioma is still hard to be treated due to their complex microenvironment. In this study, a gold nanoparticle-based delivery system was developed. The system, An-PEG-DOX-AuNPs, was loaded with doxorubicin (DOX) through hydrazone, an acid-responsive linker, and was functionalized with angiopep-2, a specific ligand of lo...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2014.10.007
更新日期:2015-01-01 00:00:00
abstract::The biological activity of osteoblasts on the newly developed bioactive poly(methyl methacrylate) (PMMA)/silica hybrid containing calcium salt was investigated. The attachment, proliferation, and differentiation of primary cultured mouse calvarial osteoblasts were evaluated by hexosaminase, MTT, and alkaline phosphata...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00421-0
更新日期:2003-03-01 00:00:00
abstract::The development of a method that can efficiently deliver nucleic acids into the nucleus of living systems remains one of the key challenges for experimental and therapeutic use of nonbiological gene delivery agents. In the current study, we demonstrate a functionalized gold nanoparticle (AuNP) that can serve as a univ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.11.074
更新日期:2011-04-01 00:00:00
abstract::Titanium surfaces with micro-nano hybrid topography (nanoscale nodules in microscale pits) have been recently demonstrated to show higher biological capability than those with microtopography alone. On the other hand, UV treatment of titanium surfaces, which is called UV photofunctionalization, has recently been intro...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.03.001
更新日期:2011-07-01 00:00:00
abstract::Ultrasoft biomaterials-polymers, gels, and human soft tissues with an elastic modulus less than ∼100 kPa-are increasingly used in medical devices. While bacterial interactions (adhesion and biofilm formation) have been extensively studied on stiffer materials, little is known about how bacteria colonize ultrasoft mate...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.04.005
更新日期:2016-07-01 00:00:00
abstract::We have shown that nanoparticles (NPs) conjugated to trans-activating transcriptor (TAT) peptide bypass the efflux action of P-glycoprotein and increase the transport of the encapsulated ritonavir, a protease inhibitor (PI), across the blood-brain-barrier (BBB) to the central nervous system (CNS). A steady increase in...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.08.004
更新日期:2008-11-01 00:00:00
abstract::Polymer-siRNA complexes (siRNA polyplexes) are being actively developed to improve the therapeutic application of siRNA. A major limitation for many siRNA polyplexes, however, is insufficient mRNA suppression. Given that modifying the sense strand of siRNA with 3' cholesterol (chol-siRNA) increases the activity of fre...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.10.019
更新日期:2011-02-01 00:00:00
abstract::Adipose-derived stem cells (ASCs) hold promise for bone regeneration but possess inferior osteogenesis potential. Allotransplantation of ASCs engineered with the BMP2/VEGF-expressing baculoviruses into rabbits healed critical-size segmental bone defects. To translate the technology to clinical applications, we aimed t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.01.052
更新日期:2015-05-01 00:00:00
abstract::Because tissues are characterized by a well-defined three-dimensional arrangement of cells, tissue engineering scaffolds that facilitate the organization and differentiation of new tissue will have improved performance in comparison to scaffolds that only provide surfaces for cell attachment and growth. We hypothesize...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.09.064
更新日期:2004-06-01 00:00:00
abstract::Hybrid drug delivery system containing both organic and inorganic nanocarriers is expected to achieve its complementary advantages for the aim of improving the performance of antineoplastic drugs in tumor therapy. Here we report the use of liposomes and gold nanoparticles to construct a liposome with a hybrid Cluster ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.004
更新日期:2016-01-01 00:00:00
abstract::Melanoma inhibitory activity (MIA), also referred to as cartilage derived retinoic acid-sensitive protein (CD-RAP), is detected physiologically in cartilage tissue and pathologically in malignant melanomas. To measure MIA/CD-RAP quantitatively we developed a sensitive ELISA system. Recently, we described diagnostic ap...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00184-4
更新日期:2003-08-01 00:00:00
abstract::Tissue-engineered skin substitutes provided a feasibility to overcome the shortage of skin autograft by culturing keratinocytes and dermal fibroblasts in vitro. In this study, we applied bi-layer gelatin-chondrointin-6-sulfate-hyaluronic acid (gelatin-C6S-HA) biomatrices onto the severe combined immunodeficiency (SCID...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.07.024
更新日期:2006-11-01 00:00:00
abstract::In order to evaluate degradation kinetics and biocompatibility of a resorbable poly(D,L-lactic acid) (PDLLA) plug for total cemented hip prostheses, an experimental in vitro and in vivo study was carried out. Degradation rate studies were performed in Ringer solution and after in vivo plug implantation in the femoral ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(97)00014-8
更新日期:1997-07-01 00:00:00
abstract::To broaden the clinical application of cartilage regenerative medicine, we should develop an implant-type tissue-engineered cartilage with firmness and 3-D structure. For that, we attempted to use a porous biodegradable polymer scaffold in the combination with atelocollagen hydrogel, and optimized the structure and co...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.02.028
更新日期:2010-06-01 00:00:00
abstract::Hepatocytes derived from human pluripotent stem cells (hPSCs) are promising candidates for cell therapy and drug discovery. However, it remains challenging to efficiently purify hepatocytes from undesired cell types after differentiation and to accurately monitor grafted cells after transplantation. Indocyanine Green ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.119431
更新日期:2019-11-01 00:00:00
abstract::The main challenge for neural progenitor cell (NPC)-mediated repair of spinal cord injury (SCI) is lack of favorable environment to direct its differentiation towards neurons rather than glial cells. The myelin associated inhibitors have been demonstrated to promote NPC differentiation into glial lineage. Herein, to i...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.03.062
更新日期:2013-07-01 00:00:00
abstract::Cell movement on adhesive surfaces is a complicated process based on myriad cell-surface interactions. Although both micron and nanoscale surface topography have been known to be important in understanding cell-materials interactions, typically only simple patterns (e.g., parallel lines or aligned posts) have been use...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.01.103
更新日期:2010-05-01 00:00:00
abstract::Atherosclerotic plaques are cholesterol-induced inflammatory niches accumulating in the vascular sub-endothelial space. Cellular and extracellular composition of human plaques is maneuvered by local inflammation that leads to alterations in the original vascular microenvironment and to the recruitment of an invading f...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.09.034
更新日期:2018-01-01 00:00:00
abstract::Riboflavin (Rf) and its metabolic analogs flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are essential for normal cellular growth and function. Their intracellular transport is regulated by the riboflavin carrier protein (RCP), which has been shown to be over-expressed by metabolically active cancer...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.08.036
更新日期:2012-12-01 00:00:00
abstract::The effects of chitin [(1 --> 4)-2-acetamido-2-deoxy-beta-D-glucan] and its partially deacetylated derivatives, chitosans, on the proliferation of human dermal fibroblasts and keratinocytes were examined in vitro. Chitosans with relatively high degrees of deacetylation strongly stimulated fibroblast proliferation whil...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00042-4
更新日期:2001-11-01 00:00:00
abstract::One of the major obstacles in drug discovery is the lack of in vitro three-dimensional (3D) models that can capture more complex features of a disease.Here we established a in vitro physiological model of the metabolic syndrome (MS) using cell-assembly technique (CAT), which can assemble cells into designated places t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.01.111
更新日期:2010-05-01 00:00:00
abstract::Biomaterials interface with toll-like receptor (TLR) 9-mediated innate immunity in a wide range of medical applications, such as tissue implants and drug delivery systems. The stimulation of TLR9 can lead to two different signaling pathways, resulting in the generation of proinflammatory cytokines (i.e. IL-6) and/or t...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.10.059
更新日期:2011-02-01 00:00:00
abstract::Decellularized extracellular matrices (ECM) from in vitro cell cultures can serve as in vivo-like matrix scaffolds for modulating cell-ECM interactions. Macromolecular crowding (MMC), the supplementation of synthetic or naturally occurring molecules resulting in excluded volume effects (EVE), has been demonstrated to ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.09.014
更新日期:2015-12-01 00:00:00