Abstract：
:The emergence of combinatorial chemistries and the increased discovery of natural compounds have led to the production of expansive libraries of drug candidates and vast numbers of compounds with potentially interesting biological activities. Despite broad interest in high throughput screening (HTS) across varied fields of biological research, there has not been an increase in accessible HTS technologies. Here, we present a simple microarray sandwich system suitable for screening chemical libraries in cell-based assays at the benchtop. The microarray platform delivers chemical compounds to isolated cell cultures by 'sandwiching' chemical-laden arrayed posts with cell-seeded microwells. In this way, an array of sealed cell-based assays was generated without cross-contamination between neighbouring assays. After chemical exposure, cell viability was analyzed by fluorescence detection of cell viability assays on a per microwell basis using a standard microarray scanner. We demonstrate the efficacy of the system by generating four hits from toxicology screens towards MCF-7 human breast cancer cells. Three of the hits were identified in a combinatorial screen of a library of natural compounds in combination with verapamil, a P-glycoprotein inhibitor. A fourth hit, 9-methoxy-camptothecin, was identified by screening the natural compound library in the absence of verapamil. The method developed here miniaturizes existing HTS systems and enables the screening of a wide array of individual or combinatorial libraries in a reproducible and scalable manner. We anticipate broad application of such a system as it is amenable to combinatorial drug screening in a simple, robust and portable platform.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Wu J,Wheeldon I,Guo Y,Lu T,Du Y,Wang B,He J,Hu Y,Khademhosseini Adoi
10.1016/j.biomaterials.2010.09.026subject
Has Abstractpub_date
2011-01-01 00:00:00pages
841-8issue
3eissn
0142-9612issn
1878-5905pii
S0142-9612(10)01181-6journal_volume
32pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Non-viral carriers for gene therapy have been developed to minimize carrier cytotoxicity and to enhance transfection efficiency. Previously, we synthesized a 9-arginine-based reducible high molecular weight peptide for gene delivery. For the reducible poly(oligo-D-arginines) (rPOA), 9-arginine oligopeptides are connec...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.08.038
更新日期：2010-12-01 00:00:00
abstract：:This study is concerned with the effect of dual implantation of calcium and phosphorus upon the structure, corrosion resistance and biocompatibility of titanium. The ions were implanted in sequence, first Ca and then P, both at a dose of 10(17) ions/cm2 at a beam energy of 25 keV. Transmission electron microscopy was ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.08.015
更新日期：2005-06-01 00:00:00
abstract：:This study concentrates on adhesion of microorganisms to polymeric biomaterials implanted in the urinary tract. A number of extensively used polymeric biomaterials (i.e., poly(hydroxyethyl-methacrylate) and its copolymers with a series of acrylic monomers--acrylic acid and dimethylaminoethyl methacrylate--polyetherure...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)89662-1
更新日期：1996-02-01 00:00:00
abstract：:Surface properties of poly (D,L-lactide) (PDLLA) were modified by combining plasma treatment and collagen modification. The changes of surface properties were characterized by contact angles, surface energy, X-ray photoelectron spectra and scanning electron microscopy. The mouse 3T3 fibroblasts were used as model cell...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00400-8
更新日期：2002-06-01 00:00:00
abstract：:We design a class of imprinted hybrid microgels that can optically monitor glucose levels with high sensitivity and selectivity in complex media at physiological pH, acting like a "glucose-indicator". Such imprinted hybrid microgels were made of Ag nanoparticles (NPs) in situ immobilized in molecularly imprinted gluco...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.06.031
更新日期：2012-10-01 00:00:00
abstract：:The influence of nanoparticles of various sizes and surface functionalities on the self-assembling fibrillogenesis of beta-amyloid (1-40) peptide was investigated. Functionalized nanoparticles including quantum dots and gold nanoparticles were co-incubated with monomeric Aβ(1-40) peptides under seed-mediated growth me...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.03.024
更新日期：2012-06-01 00:00:00
abstract：:In vitro experiments were conducted upon some common metallic biomaterials and carbons, both isolated or forming galvanic couples, in a cell specially designed for crevice corrosion studies. The alloys examined were AISI 316L stainless steel, Ti6AI4V and Co-Cr-Mo. The types of carbon were glassy carbon and carbon fibr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(90)90110-c
更新日期：1990-07-01 00:00:00
abstract：:The in vivo evaluation, in New Zealand rabbits, of a SiO(2)-P(2)O(5)-CaO sol-gel glass and a SiO(2)-P(2)O(5)-CaO-MgO glass-ceramic, both bioactive in Kokubo's simulated body fluid (SBF), is presented. Bone defects, performed in the lateral aspect of distal right femoral epiphysis, 5mm in diameter and 4mm in depth, wer...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.12.009
更新日期：2004-08-01 00:00:00
abstract：:Atherosclerotic plaques are cholesterol-induced inflammatory niches accumulating in the vascular sub-endothelial space. Cellular and extracellular composition of human plaques is maneuvered by local inflammation that leads to alterations in the original vascular microenvironment and to the recruitment of an invading f...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.09.034
更新日期：2018-01-01 00:00:00
abstract：:The aim of this study was to identify and quantify potential regional and directional variations in the quasistatic uniaxial mechanical properties of the passive urinary bladder wall. Overall, the lower body and trigone regions demonstrated the highest degree of directional anisotropy, whereas the ventral region demon...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.09.034
更新日期：2009-01-01 00:00:00
abstract：:Although transplantation of pancreatic islets is a promising approach for treatment of type 1 diabetes mellitus, the engraftment efficiency of these islets is limited by host immune responses. Extensive efforts have been made to immunoisolate these islets by introducing barriers on the islet surface. To date, these ba...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.08.017
更新日期：2018-11-01 00:00:00
abstract：:Embryonic stem cells (ESCs) are promising cell sources for cell-based therapy. It has been established that the formation of ESC aggregates promotes their differentiation into the derivatives of all three germ layers. ESC aggregates are generally prepared via the formation of suspended spherical aggregates called embr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.05.003
更新日期：2009-09-01 00:00:00
abstract：:Calcium phosphates (CaP) and phosphate-based glass (P-glass, xCaO-(0.55-x) Na(2)O-0.45P(2)O(5) composition) composite coatings were obtained on a strong ZrO(2) to improve biocompatibility, the mechanical strength and biological activity. Hydroxyapatite (HA) and P-glass mixed powder slurries were coated on the ZrO(2) s...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.10.094
更新日期：2004-08-01 00:00:00
abstract：:Three critical aspects of searching for and understanding how to find highly resistant surfaces to protein adhesion are addressed here with specific application to synthetic membrane filtration. They include the (i) discovery of a series of previously unreported monomers from a large library of monomers with high prot...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.11.001
更新日期：2012-02-01 00:00:00
abstract：:Here we document the complete process of bioresorption and bone replacement of rods made of forged composites of unsintered hydroxyapatite particles/poly l-lactide (F-u-HA/PLLA) implanted in the femoral medullary cavities of rabbits. Bioresorption, osteoconductive bioactivity and bone replacement were compared in thre...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.02.016
更新日期：2005-09-01 00:00:00
abstract：:Recent advances in efficient microRNA (miRNA) delivery techniques using brain-targeted nanoparticles offer critical information for understanding the functional role of miRNAs in vivo, and for supporting targeted gene therapy in terms of treating miRNA-associated neurological diseases. Here, we report the rabies virus...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.03.047
更新日期：2011-07-01 00:00:00
abstract：:For a temporomandibular joint prosthesis, an estimation of the wear rate was needed, prior to patient application. Therefore, we determined the in vitro wear rate of the ball-socket articulation of this prosthesis, consisting of a metal head and an ultra-high molecular weight polyethylene (UHMWPE) cup. The basic testi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00042-3
更新日期：1999-08-01 00:00:00
abstract：:Multifunctional and water-soluble superparamagnetic iron oxide (SPIO) nanocarriers were developed for targeted drug delivery and positron emission tomography/magnetic resonance imaging (PET/MRI) dual-modality imaging of tumors with integrin α(v)β₃ expression. An anticancer drug was conjugated onto the PEGylated SPIO n...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.02.006
更新日期：2011-06-01 00:00:00
abstract：:3D printing is known as a cost-effective technique that shows huge potential in fabrication of graft substitutes for bone tissue regeneration. However, the tradeoff between 3D printability, mechanical strength and bioactivity of the printed materials (i.e., inks) remains a challenge. In this work, we present a novel p...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120378
更新日期：2020-12-01 00:00:00
abstract：:Current tissue engineering strategies are focused on the restoration of pathologically altered tissue architecture by transplantation of cells in combination with supportive scaffolds and biomolecules. In recent years, considerable attention has been given to chitosan (CS)-based materials and their applications in the...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2005.03.016
更新日期：2005-10-01 00:00:00
abstract：:We genetically introduced the Fc-binding peptide (FcBP) into the loop of a self-assembled protein cage, ferritin, constituting four-fold symmetry at the surface to use it as a modular delivery nanoplatform. FcBP-presenting ferritin (FcBP-ferritin) formed very stable non-covalent complexes with both human and rabbit Ig...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.03.055
更新日期：2012-07-01 00:00:00
abstract：:A method to fabricate porous, biodegradable conduits using a combined injection molding, thermally induced phase transition technique was developed which produced conduits with dimensionally toleranced, longitudinally aligned channels. The geometry of the channels was designed to approximate the architecture of periph...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00409-x
更新日期：2003-02-01 00:00:00
abstract：:Potential applications of engineered, functional, cardiac muscle extends from basic research through drug discovery to engineering heart tissue for transplantation. One of the central questions in cardiac tissue engineering is to understand and control the complex interactions between the cardiac muscle cells and thei...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.01.020
更新日期：2004-11-01 00:00:00
abstract：:The effect of PEGylation on the aqueous solution properties and cell membrane disruptive activity of a pH-responsive pseudo-peptide, poly(l-lysine iso-phthalamide), has been investigated by dynamic light scattering, haemolysis and lactate dehydrogenase (LDH) assays. Intracellular trafficking of the polymers has been e...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.07.040
更新日期：2008-11-01 00:00:00
abstract：:This paper is concerned with the entrapment of an enzyme within an aggregated metallic matrix and the development of a bioactive enzyme-metal composite. Whereas the use of organic polymers and metal oxides for the preparation of enzymatically active materials is well developed, the third principle enzyme-material comb...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.11.026
更新日期：2009-03-01 00:00:00
abstract：:Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transf...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.05.070
更新日期：2011-10-01 00:00:00
abstract：:Normal functioning of articulating tissues is required for many physiological processes occurring across length scales from the molecular to whole organism. Lubricating biopolymers are present natively on tissue surfaces at various sites of biological articulation, including eyelid, mouth, and synovial joints. The ran...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2018.07.040
更新日期：2018-10-01 00:00:00
abstract：:Carbon nanotube (CNT) possesses excellent properties as a drug carrier. To overcome the challenge of drug functionalization with CNT, we have developed a lipid-drug approach for efficient drug loading onto CNT, in which a long chain lipid molecule is conjugated to the drug molecule so that the lipid-drug can be loaded...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.09.007
更新日期：2013-12-01 00:00:00
abstract：:Gold nanoparticles hold great promise as anti-cancer theranostic agents against cancer by actively targeting the tumor cells. As this potential has been supported numerously during in vitro experiments, the effective application is hampered by our limited understanding and control of the interactions within complex in...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.01.007
更新日期：2017-04-01 00:00:00
abstract：:Although silicones possess many useful properties as biomaterials, their hydrophobicity can be problematic. To a degree, this issue can be addressed by surface modification with hydrophilic polymers such as poly(ethylene glycol), but the resulting structures are usually not conducive to cell growth. In the present wor...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.01.069
更新日期：2010-05-01 00:00:00
