Abstract:
:Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Lee CH,Kim JH,Lee HJ,Jeon K,Lim H,Choi Hy,Lee ER,Park SH,Park JY,Hong S,Kim S,Cho SGdoi
10.1016/j.biomaterials.2011.05.070subject
Has Abstractpub_date
2011-10-01 00:00:00pages
6683-91issue
28eissn
0142-9612issn
1878-5905pii
S0142-9612(11)00641-7journal_volume
32pub_type
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