Abstract:
:Atherosclerotic plaques are cholesterol-induced inflammatory niches accumulating in the vascular sub-endothelial space. Cellular and extracellular composition of human plaques is maneuvered by local inflammation that leads to alterations in the original vascular microenvironment and to the recruitment of an invading fibrous layer (fibroatharoma). In the present study we introduce a bioengineered three-dimensional model of human fibroatheroma (ps-plaque) assembled with a tailored hanging-drop protocol. Using vi-SNE based multidimensional flow cytometry data analysis we compared the myeloid cell-populations in ps-plaques to those in plaques isolated from human carotid arteries. We observed that plasmacytoid and activated dendritic cells are the main myeloid components of human carotid plaques and that both cell types are present in the biofabricated model. We found that low-density lipoproteins affect cell viability and contribute to population polarization in ps-plaques. The current work describes the first human bioengineered in vitro model of late atherosclerotic lesion for the investigation of atherosclerosis aetiopathogenesis.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Mallone A,Stenger C,Von Eckardstein A,Hoerstrup SP,Weber Bdoi
10.1016/j.biomaterials.2017.09.034subject
Has Abstractpub_date
2018-01-01 00:00:00pages
49-59eissn
0142-9612issn
1878-5905pii
S0142-9612(17)30626-9journal_volume
150pub_type
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