Abstract：
:A facile and efficient method to immobilize bioactive proteins onto polymeric substrate was established. Testis-specific protease 50 (TSP50) was immobilized on ultrafine biodegradable polymer fibers, i.e., (1) to prepare a propargyl-containing polymer P(LA90-co-MPC10) by introducing propargyl group into a cyclic carbonate monomer (5-methyl-5-propargyloxycarbonyl-1,3-dioxan-2-one, MPC) and copolymerizing it with l-lactide; (2) to electrospin the functionalized polymer into ultrafine fibers; (3) to azidize the TSP50, and (4) to perform the click reaction between the propargyl groups on the fibers and the azido groups on the protein. The TSP50-immobilized fibers can resist non-specific protein adsorptions but preserve specific recognition and combination with anti-TSP50. ELISA tests were carried out by using HRP-goat-anti-mouse-IgG(H+L) as secondary antibody and o-phenylenediamine (OPDA)/H(2)O(2) as substrate to detect the combination of immobilized TSP50 with anti-TSP50. The results showed that anti-TSP50 can be selectively adsorbed from its solution onto the TSP50-immobilized fibers in the presence of BSA of as high as 10(4) times concentration. TSP50 immobilized on the fiber and anti-TSP50 combined to the fiber were also quantitatively determined. Anti-TSP50 can be then eluted off from the fiber when pH changes. The eluted fiber can re-combine anti-TSP50 at an efficiency of 75% compared to the original TSP50-immobilized fiber. Therefore, the TSP50-immobilized fibers can be used in the detection, separation, and purification of anti-TSP50. The "click" method can lead to a universal strategy to protein immobilization.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Shi Q,Chen X,Lu T,Jing Xdoi
10.1016/j.biomaterials.2007.11.008subject
Has Abstractpub_date
2008-03-01 00:00:00pages
1118-26issue
8eissn
0142-9612issn
1878-5905pii
S0142-9612(07)00897-6journal_volume
29pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Layered double hydroxide (LDH) nanoparticles are attractive as potential drug vectors for the targeting not only of tissues, but also of intracellular organelles, and particularly the acidic endolysosomes created after cell endocytosis. The purpose of this study was to investigate the ability of LDH nanoparticles desi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.09.095
更新日期：2010-02-01 00:00:00
abstract：:The goal of this study was to develop a polymeric carrier for delivery of anti-tumor drugs and sustained release of these agents in order to optimize anti-tumor activity while minimizing systemic effects. We used oligo(poly(ethylene glycol) fumarate) (OPF) hydrogels modified with small negatively charged molecules, so...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.06.054
更新日期：2010-11-01 00:00:00
abstract：:Time-dependent energetics of blood-protein adsorption are interpreted in terms of a slowly-concentrating three-dimensional interphase volume initially formed by rapid diffusion of protein molecules into an interfacial region spontaneously formed by bringing a protein solution into contact with a physical surface. This...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.03.043
更新日期：2008-07-01 00:00:00
abstract：:In vitro tissue engineered models are poised to have significant impact on disease modeling and preclinical drug development. Reliable methods to induce microvascular networks in such microphysiological systems are needed to improve the size and physiological function of these models. By systematically engineering sev...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.119921
更新日期：2020-06-01 00:00:00
abstract：:A glucose biosensor was fabricated with loading of glucose oxidase (GOx) into a new organic-inorganic hybrid nanocomposite. The preparation involves formation of silica network into a Nafion (perfluorosulfonate ionomer) and subsequent loading of polyaniline grafted multiwalled carbon nanotubes (MWNT-g-PANI) onto Nafio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.07.047
更新日期：2009-10-01 00:00:00
abstract：:AuCu and AuAg alloys (30 at. % Au) have been immersed in aqueous solutions of chloride and sulphide with additions of protein (mucin). The surfaces of the alloys were subsequently analysed by means of ESCA and compared with previous and similar tests without addition of mucin. No noticeable effect of the mucin additio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(85)90099-7
更新日期：1985-11-01 00:00:00
abstract：:The calcination and natural sintering of calcium deficient hydroxyapatite powders Ca(10-x)(PO4)(6-x)(HPO4)x(OH)(2-x) (with 0 < or = x < or = 1) were studied. For temperature below 700 degrees C, particle coalescence occurs without densification. The particle coalescence is associated with a reduction of the specific s...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00219-8
更新日期：2002-02-01 00:00:00
abstract：:New therapeutic strategies are needed for the growing unmet clinical needs in liver disease and fibrosis. Platelet activation and PDGF activity are recognized as important therapeutic targets; however, no therapeutic approach has yet addressed these two upstream drivers of liver fibrosis. We therefore designed a matri...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120538
更新日期：2020-11-17 00:00:00
abstract：:Immunotherapy has shown promising treatment effects for a variety of cancers. However, the immune treatment efficiency for solid tumors is limited owing to insufficient infiltration of immune cells into solid tumors. The conversion of tumor-supportive macrophages to tumor-suppressive macrophages, inducing the function...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.09.034
更新日期：2017-01-01 00:00:00
abstract：:Concerns over disease transmission, high costs and limited supply have led to interest in synthetic grafts in the field of impaction bone grafting (IBG). Poly(DL-lactic acid) (PLA) grafts are attractive alternatives due to their biocompatibility, established safety and versatile manufacturing process. This study exami...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.04.017
更新日期：2008-08-01 00:00:00
abstract：:Bioactive coatings for neural electrodes that are tailored for cell interactions have the potential to produce superior implants with improved charge transfer capabilities. In this study synthetically produced anionically modified laminin peptides DEDEDYFQRYLI and DCDPGYIGSR were used to dope poly(3,4-ethylenedioxythi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.03.043
更新日期：2009-08-01 00:00:00
abstract：:The surface of soft contact lenses made of crosslinked poly(2-hydroxyethyl methacrylate). pHEMA, has been investigated with atomic force microscopy in contact mode. The friction force and adhesive force measurements were able to differentiate the non-crosslinked pHEMA chains from the surface of the crosslinked pHEMA n...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00175-2
更新日期：2001-12-01 00:00:00
abstract：:Myoepithelial cells are flat, stellate cells present in exocrine tissues including the salivary glands. While myoepithelial cells have been studied extensively in mammary and lacrimal gland tissues, less is known of the function of myoepithelial cells derived from human salivary glands. Several groups have isolated tu...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.07.022
更新日期：2017-10-01 00:00:00
abstract：:The initial stages of the in vitro degradation of and the drug release from a matrix made of poly(d,l-lactide-co-glycolide) was carried out in a phosphate buffer saline (pH 7.0) medium. It has been observed that substantial matrix degradation occurs at the end of 2 weeks of immersion. The drug release using films of t...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00597-0
更新日期：2004-02-01 00:00:00
abstract：:Replacing the tissue lost after a stroke potentially provides a new neural substrate to promote recovery. However, significant neurobiological and biotechnological challenges need to be overcome to make this possibility into a reality. Human neural stem cells (hNSCs) can differentiate into mature brain cells, but requ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.06.085
更新日期：2012-10-01 00:00:00
abstract：:Oxidative stress, resulting from excessive generation of reactive oxygen species (ROS), plays a pivotal role in the initiation and progression of inflammatory bowel disease (IBD). To develop an efficacious and safe nanotherapy against IBD, we designed and developed a superoxide dismutase/catalase mimetic nanomedicine ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.08.010
更新日期：2016-10-01 00:00:00
abstract：:One of the main issues in orthopaedic implant design is the fabrication of scaffolds that closely mimic the biomechanical properties of the surrounding bone. This research reports on a multi-stage rapid prototyping technique that was successfully developed to produce porous titanium scaffolds with fully interconnected...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.05.032
更新日期：2008-09-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC), as the fifth most common malignant cancer, develops and progresses mostly in a cirrhotic liver where stiff nodules are separated by fibrous bands. Scaffolds that can provide a 3D cirrhotic mechanical environment with complex native composition and biomimetic architecture are necessary f...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.09.026
更新日期：2018-12-01 00:00:00
abstract：:Self-assembly is a prominent phenomenon observed in nature. Inspired by this thermodynamically favorable approach, several natural and synthetic materials have been investigated to develop functional systems for various biomedical applications, including drug delivery. Furthermore, layered self-assembled systems provi...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2020.119929
更新日期：2020-02-29 00:00:00
abstract：:Extracellular matrix (ECM) based biomaterials have an established place as medical devices for wound healing and tissue regeneration. In the search for biomaterials we have identified ovine forestomach matrix (OFM), a thick, large format ECM which is biochemically diverse and biologically functional. OFM was purified ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.02.025
更新日期：2010-06-01 00:00:00
abstract：:We describe a novel approach to process bioceramic microparticles and poly(diol citrates) into bioceramic-elastomer composites for potential use in orthopedic surgery. The composite consists of the biodegradable elastomer poly(1,8-octanediol-citrate) (POC) and the bioceramic hydroxyapatite (HA). The objective of this ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.07.042
更新日期：2006-12-01 00:00:00
abstract：:In-stent restenosis is one of the most serious modes of failure of cardiovascular stent implant. Although drug-eluting stents have been proven to reduce in-stent restenosis, the nonspecific inhibitory effects of anti-proliferative drugs, such as rapamycin, result in delayed re-endothelialization and fatal late stent t...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.11.042
更新日期：2017-02-01 00:00:00
abstract：:Acute and chronic wounds affect millions and are associated with billions of dollars in healthcare costs. The use of healing markers, biochemical cues from biocompatible matrices and materials, and their correlation with wound healing has the potential to generate valuable diagnostic, prognostic, and therapeutic infor...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.05.007
更新日期：2019-08-01 00:00:00
abstract：:Hypoxia, as an inevitable characteristic of solid tumors, has been regarded as a noticeably causative factor to therapeutic resistance and metastatic variants. Exploring novel theranostics to realize the accurate diagnosis of hypoxia and the simultaneous implementation of effective therapy is a promising prospect for ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.05.018
更新日期：2018-08-01 00:00:00
abstract：:We have developed an ion-sensing nanoparticle that is comprised of a superparamagnetic iron oxide (SPIO) core encapsulated with a porous silica shell. The latter can be readily anchored with ligands capable of coordinating with positron-emitting metal. Evidently, this nanoparticle has a great potential for use in cell...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.12.025
更新日期：2010-04-01 00:00:00
abstract：:Metal particles and ions from hip prostheses have the potential to induce the production of reactive oxygen species (ROS), making them prime suspects for disturbing the cellular balance of oxidants/antioxidants (redox state of the cell). To better understand the cellular effect of metal ions from metal-on-metal prosth...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.11.019
更新日期：2005-07-01 00:00:00
abstract：:There is great demand for constructing well-organized three-dimensional (3D) tissues in vitro. Here, we developed a 3D stacked culture method using biodegradable poly(d,l-lactide-co- glycolide) (PLGA) membranes with defined topography. Pore size and porosity of the membranes can be controlled by changing the moisture ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.12.039
更新日期：2012-03-01 00:00:00
abstract：:Implantation of a medical implant within the body inevitably triggers a host inflammatory response that negatively impacts its function and longevity. Nevertheless, the degree and severity of this response may be reduced by selecting appropriate materials, implant geometry, surface topography and surface treatment. He...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.01.005
更新日期：2015-04-01 00:00:00
abstract：:Here, we demonstrated dimethyldioctadecylammonium bromide (DODAB), a cationic lipid, bilayer coated Au nanoparticles (AuNPs) could efficiently deliver two types of plasmid DNA into human embryonic kidney cells (HEK 293) in the presence of serum. The transfection efficiency of AuNPs was about five times higher than tha...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.05.020
更新日期：2008-09-01 00:00:00
abstract：:The objective of this study was to investigate the moisture absorption, physical-mechanical and bioadhesive properties of hot-melt extruded hydroxypropylcellulose (HPC) films containing polymer additives. These additives included polyethylene glycol (PEG) 5%, polycarbophil 5%, carbomer 5%, Eudragit E-100 5%, and sodiu...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00046-6
更新日期：2000-07-01 00:00:00
