Abstract:
:Grape seed proanthocyanidin extract (GSPE), a polyphenolic compound with antioxidant properties, may protect against cardiac ischemia and reperfusion injury. However, its potential toxicity at higher doses is unknown. The authors tested the effects of GSPE on reactive oxygen species (ROS) generation, cell survival, lactate dehydrogenase (LDH) release, and caspase- 3 activity using chick cardiomyocytes incubated with GSPE at 5, 10, 50, 100, or 500 micrograms/mL in medium for 8 h. Exposure to increasing concentrations of GSPE (100 or 500 micrograms/mL) resulted in an increase in ROS generation and cell death as measured by propidium iodide uptake and LDH release. Caspase-3 activity was significantly increased fourfold in cells exposed to GSPE 500 micrograms/ mL compared to controls; this was abolished by the selective caspase-3 inhibitor Ac-Asp-Gln-Thr-Asp-H (50 microM), which also significantly reduced the cell death resulting from GSPE (500 micrograms/mL). The antioxidant N-acetylcysteine (NAC, 100 microM) reduced cell death induced by GSPE (500 micrograms/mL) but failed to attenuate caspase-3 activation. Collectively, the authors conclude that higher doses of GSPE could cause apoptotic cell injury via effector caspase-3 activation and subsequent induction of ROS generation. Consumers may take higher doses of dietary supplements in the belief that natural herbs have no major side effects. This study demonstrates that dosages of GSPE should be optimized to avoid potential harmful pro-oxidant effects.
journal_name
Cardiovasc Toxicoljournal_title
Cardiovascular toxicologyauthors
Shao ZH,Vanden Hoek TL,Xie J,Wojcik K,Chan KC,Li CQ,Hamann K,Qin Y,Schumacker PT,Becker LB,Yuan CSdoi
10.1385/ct:3:4:331keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
331-9issue
4eissn
1530-7905issn
1559-0259pii
CT:3:4:331journal_volume
3pub_type
杂志文章abstract::We present an experimental approach that allows exposure of cells plated on a single coverslip to multiple distinct environments. The original chamber design created a small region of injury using geometrically defined flows of the control and ischemic solutions. Modifications of the original chamber design presented ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:4:1:01
更新日期:2004-01-01 00:00:00
abstract::Acute alcohol intoxication leads to an inhibition of protein synthesis in heart that results in part through altered phosphorylation of protein factors controlling mRNA translation initiation. The purpose of the present set of experiments was designed to examine the effects of inhibitors of ethanol metabolism on the p...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-008-9012-4
更新日期:2008-03-01 00:00:00
abstract::This study investigated if the cardioprotective effect of acylated ghrelin (AG) against doxorubicin (DOX)-induced cardiac toxicity in rats involves inhibition of Fas/FasL-mediated cell death. It also investigated if such an effect is mediated by restoring Ca+2 homeostasis from the aspect of stimulation of SERCA2a rece...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-019-09527-8
更新日期:2019-12-01 00:00:00
abstract::Over 300,000 hip replacements occurred in the USA in 2010, and the frequency is likely increasing annually. Blood Cobalt (Co) concentrations in patients with well-functioning cobalt-chromium (Co-Cr) hip implants are usually elevated above background concentrations relative to the general population. Excessive Co expos...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-017-9433-z
更新日期:2018-06-01 00:00:00
abstract::Clopidogrel is used in patients with coronary syndromes and at risk of thrombotic events or receiving percutaneous coronary intervention (PCI) for reducing heart attack and stroke. Here we present genotype and phenotype study of Iranian patients undergoing PCI treated with clopidogrel during a 6-month period of follow...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-018-9459-x
更新日期:2018-10-01 00:00:00
abstract::Dofetilide is a class III antiarrhythmic used for treating atrial dysrhythmias. Though its adverse effects are well described in routine use, very little is known about dofetilide toxicity in overdose. This is a retrospective case series of consecutive patients reported to our poison center after dofetilide overdose. ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-016-9384-9
更新日期:2017-07-01 00:00:00
abstract::The present study was undertaken to evaluate protective role of S. rhomboidea. Roxb (SR) leaf extract against in vitro low-density lipoprotein (LDL) oxidation and oxidized LDL (Ox-LDL) induced macrophage apoptosis. Copper and cell-mediated LDL oxidation, Ox-LDL-induced peroxyl radical generation, mitochondrial activit...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-011-9110-6
更新日期:2011-06-01 00:00:00
abstract::We have compared the cardiotoxicity of five platinum complexes in a model of isolated rat heart using the Langendorff technique. These effects were assessed via coronary flow (CF) and cardiac functional parameters. cis-Diamminedichloroplatinum(II) (cisplatin, CDDP), dichloro-(1,2-diaminocyclohexane)platinum(II) (Pt((I...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-014-9293-8
更新日期:2015-07-01 00:00:00
abstract::Cardiac troponins not only allow for risk stratification and guidance of therapy in unstable angina and non-Q-wave acute myocardial infarction but also may be useful in the diagnostic workup and monitoring of patients with ST-segment elevation myocardial infarction. In clinical practice, troponins are used for confirm...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1385/ct:1:2:099
更新日期:2001-01-01 00:00:00
abstract::Our aim is to investigate the correlation between 9p21 chromosome rs4977574 polymorphism genotypes and the development of coronary artery heart disease (CHD). Two hundred and eighty-nine patients with angiography-confirmed CHD were recruited as the CHD group, while 338 subjects without CHD symptoms were enrolled as th...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-016-9372-0
更新日期:2017-04-01 00:00:00
abstract::Arsenic toxicity is one of the major environmental problems causing various diseases, cardiovascular disorders is one of them. Several epidemiological studies have shown that arsenic causes cardiac hypertrophy but the detailed molecular mechanism is to be studied yet. This study is designed to determine the molecules ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-019-09550-9
更新日期:2020-06-01 00:00:00
abstract::Heart failure is a common complication of doxorubicin (DOX) therapy. Previous studies have shown that DOX adversely impacts cardiac energy metabolism, and the ensuing energy deficiencies antedate clinical manifestations of cardiac toxicity. Brief exposure of cultured cardiomyocytes to DOX significantly decreases creat...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-014-9283-x
更新日期:2015-04-01 00:00:00
abstract::Four decades of research on the link between hyperhomocysteinemia and cardiovascular disease has led to a crossroads. Several negative studies on the role of homocysteine-lowering B-vitamin therapy in reducing the risk of atherothrombotic cardiovascular disease have dampened enthusiasm for this important field of rese...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-009-9042-6
更新日期:2009-06-01 00:00:00
abstract::DLBS1425 is a bioactive compound extracted from Phaleria macrocarpa, with anti-proliferative, anti-inflammatory and anti-angiogenic properties against cancer cells. The present study was aimed to assess cardiotoxicity of DLBS1425, compared to the mainstay regimen for breast cancer, 5-fluorouracil:doxorubicin:cyclophos...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-014-9275-x
更新日期:2015-01-01 00:00:00
abstract::Present study evaluates cardioprotective role of melatonin (Mel), alpha lipoic acid (ALA), a combination of melatonin and alpha lipoic acid (Mel + ALA) against cadmium (Cd)-induced oxidative damage. Female albino rats were subjected to 15-day exposure to Cd (5.12 mg/kg bw) alone or treated with ML (10 mg/kg bw) + ALA ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-010-9092-9
更新日期:2011-03-01 00:00:00
abstract::HIV infection in patients is associated with a surprisingly high frequency of diastolic dysfunction followed by the development of a dilated cardiomyopathy. Potential mechanisms include direct effects of HIV proteins, including gp120. We have previously reported direct inotropic and p38 MAP kinase signaling effects of...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-009-9047-1
更新日期:2009-09-01 00:00:00
abstract::The anticancer drug lapatinib (Tykerb) is a dual tyrosine kinase inhibitor targeting the HER2 (ERBB2) and EGFR (ERBB1, HER1) pathways that have been shown in clinical trials to display some cardiotoxicity. Because trastuzumab also targets HER2 receptors, the lapatinib/doxorubicin combination provides a good model to p...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-012-9183-x
更新日期:2013-03-01 00:00:00
abstract::This study was aimed at evaluation of changes in activities of selected antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase) and contents of key nonenzymatic antioxidants (glutathione, protein thiol groups, and α- and γ-tocopherols) in the left heart ventricle of youn...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-011-9105-3
更新日期:2011-06-01 00:00:00
abstract::Intracellular Na(+) ([Na(+)](i)) is an important modulator of excitation-contraction coupling via regulating Ca(2+) efflux/influx, and no investigation has been so far performed in diabetic rat heart. Here, we examined whether any change of [Na(+)](i) in paced cardiomyocytes could contribute to functional alterations ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-012-9192-9
更新日期:2013-06-01 00:00:00
abstract::Our previous study described the cardioprotective effects of ellagic acid in an isoproterenol-induced myocardial infarction model. In this study, we are reporting the mechanism of protective action of ellagic acid with respect to apoptosis and mitochondrial respiratory enzymes. Ellagic acid (7.5 and 15 mg/kg) was admi...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-011-9138-7
更新日期:2012-03-01 00:00:00
abstract::We report a case of potential cardiovascular toxicity including syncope, bradycardia, and ECG pauses associated with the use of rivastigmine and atenolol. A 65-year-old African American female with a medical history of dementia, hypertension, seizure disorder, stroke, and peripheral vascular disease was admitted to th...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-010-9088-5
更新日期:2010-12-01 00:00:00
abstract::Asiatic acid (AA), a pentacyclic triterpenoid, is a key phytoconstituent of Centella asiatica. AA is a patented as a cytotoxic substance, and it exerts cytotoxicity against Hep3B, Hela and MCF-7 cell lines. However, pentacyclic triterpenoids also modulate the expression and transcriptional activities of Nrf2 and exert...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-017-9424-0
更新日期:2018-04-01 00:00:00
abstract::The corticotrophin-releasing hormone-related factor, urocortin (Ucn) and the interleukin (IL)-6 family cytokine cardiotrophin-1 (CT-1) are both cardioprotective agents able to protect the heart from ischemic damage. In both cases the protective effect involves activation of the p42/p44 MAPK and PI-3 kinase/Akt pathway...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:3:3:255
更新日期:2003-01-01 00:00:00
abstract::Serotonergic drugs may lead to valvular heart disease in humans and more recently also in rats. Although clinical data suggest that dose dependency and reversibility after drug cessation might occur, proof of this is lacking. For that purpose, a total of 106 rats were prospectively enrolled: 22 control animals and 7 g...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-009-9046-2
更新日期:2009-09-01 00:00:00
abstract::Fresh vehicular emissions potentially represent a ubiquitous environmental concern for cardiovascular health. We compared electrocardiographic effects of fresh gasoline engine emissions with resuspended paved road dust in a mouse model of coronary insufficiency. Apolipoprotein E (ApoE)-/- mice on a high fat diet were ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:6:3:199
更新日期:2006-01-01 00:00:00
abstract::It is generally considered that the genetics of atherosclerosis and its complications involves a large number of genes with common alleles having weak effects on disease risk but possibly interacting with each other and with non-genetic factors. In such a complex system, absence of marginal effects (effects of polymor...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1385/ct:5:2:143
更新日期:2005-01-01 00:00:00
abstract::Heart rate variability (HRV) is a measure of cardiac pacing dynamics that has recently garnered a great deal of interest in environmental health studies. While the use of these measures has become popular, much uncertainty remains in the interpretation of results, both in terms of human and animal research. In humans,...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-007-0004-6
更新日期:2007-01-01 00:00:00
abstract::The effects of magnesium (Mg2+) on ischaemic complications of pathological cardiac hypertrophy are unclear. In this study, we investigated effects of Mg2+ pretreatment on ischaemia/reperfusion (I/R) injury in isoprenaline (ISO)-induced hypertrophic hearts. Wistar rats were treated for 7 days with different combination...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-015-9355-6
更新日期:2017-01-01 00:00:00
abstract::Cardiac toxicity has been associated with HIV infection and exposure to nucleoside reverse transcriptase inhibitors (NRTIs), but the role of the latter in the development of cardiac disease of HIV-infected patients is uncertain. To investigate the cardiotoxicity of transplacentally administered zidovudine (AZT) or AZT...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:4:2:133
更新日期:2004-01-01 00:00:00
abstract::Although the role of tropomyosin is well-defined in striated muscle, the precise mechanism of how tropomyosin functions is still unclear. It has been shown that extension of either N- or C-terminal ends of sarcomeric tropomyosin do not affect cardiac myofibrillogenesis, but it is not known whether simultaneous extensi...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:5:1:001
更新日期:2005-01-01 00:00:00