Abstract:
:Intracellular Na(+) ([Na(+)](i)) is an important modulator of excitation-contraction coupling via regulating Ca(2+) efflux/influx, and no investigation has been so far performed in diabetic rat heart. Here, we examined whether any change of [Na(+)](i) in paced cardiomyocytes could contribute to functional alterations during diabetes. Slowing down in depolarization phase of the action potential, small but significant decrease in its amplitude with a slight depolarized resting membrane potential was traced in live cardiomyocytes from diabetic rat, being parallel with a decreased TTX-sensitive Na(+) channel current (I(Na)) density. We recorded either [Na(+)](i) or [Ca(2+)](i) by using a fluorescent Na(+) indicator (SBFI-AM or Na-Green) or a Ca(2+) indicator (Fura 2-AM) in freshly isolated cardiomyocytes. We examined both [Na(+)](i) and [Ca(2+)](i) at rest, and also [Na(+)](i) during pacing with electrical field stimulation in a range of 0.2-2.0 Hz stimulation frequency. In order to test the possible contribution of Na(+)/H(+) exchanger (NHE) to [Na(+)](i), we examined the free cytoplasmic [H(+)](i) changes from time course of [H(+)](i) recovery in cardiomyocytes loaded with SNARF1-AM by using ammonium prepulse method. Our data showed that [Na(+)](i) in resting cells from either diabetic or control group was not significantly different, whereas the increase in [Na(+)](i) was significantly smaller in paced diabetic cardiomyocytes compared to that of the controls. However, resting [Ca(2+)](i) in diabetic cardiomyocytes was significantly higher than that of the controls. Here, a lower basal pH(i) in diabetics compared with the controls correlates also with a slightly higher but not significantly different NHE activity and consequently a similar Na(+) loading rate at resting state with a leftward shift in pH sensitivity of NHE-dependent H(+)-flux. NHE protein level remained unchanged, while protein levels of Na(+)/K(+) ATPase and Na(+)/Ca(2+) exchanger were decreased in the diabetic cardiomyocytes. Taken together, the present data indicate that depressed I(Na) plays an important role in altered electrical activity with less Na(+) influx during contraction, and an increased [Ca(2+)](i) load in these cells seems to be independent of [Na(+)](i). The data with insulin treatment suggest further a recent balance between Na(+) influx and efflux proteins associated with the [Na(+)](i), particularly during diabetes.
journal_name
Cardiovasc Toxicoljournal_title
Cardiovascular toxicologyauthors
Bilginoglu A,Kandilci HB,Turan Bdoi
10.1007/s12012-012-9192-9subject
Has Abstractpub_date
2013-06-01 00:00:00pages
138-47issue
2eissn
1530-7905issn
1559-0259journal_volume
13pub_type
杂志文章abstract::The present study was designed to evaluate the cardioprotective potential of water extract of rhizomes of Acorus gramineus (AGR) against isoproterenol (ISO)-induced myocardial infarction. Male pigs were orally administered with 250 or 500 mg/kg of AGR or with vehicle for 9 days, with concurrent subcutaneous injections...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-014-9243-5
更新日期:2014-06-01 00:00:00
abstract::Although the role of tropomyosin is well-defined in striated muscle, the precise mechanism of how tropomyosin functions is still unclear. It has been shown that extension of either N- or C-terminal ends of sarcomeric tropomyosin do not affect cardiac myofibrillogenesis, but it is not known whether simultaneous extensi...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:5:1:001
更新日期:2005-01-01 00:00:00
abstract::Acute cardiovascular poisoning is a major cause of adverse outcomes in poisoning emergencies. The prognostic validity of corrected QT (QTc) and dispersed QT (QTd) in these outcomes is still limited. The present study aimed to determine the risk factors of mortality, adverse cardiovascular events (ACVE), and intensive ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-020-09629-8
更新日期:2021-01-05 00:00:00
abstract::Choerospondias axillaris (Guangzao) is a medicinal plant used in Mongolia, and its fruit is commonly used for the treatment of cardiovascular diseases in clinic. The constituents responsible for this effect are always conceded to be the total flavonoids of C. axillaries (TFC). The present study was to evaluate the pre...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-013-9238-7
更新日期:2014-06-01 00:00:00
abstract::Carbon soot is produced in the process of diamond manufacture. Recent experimental and epidemiological studies have associated exposure to carbon nanoparticles with effects on cardiovascular system and blood cells. The objective of this study is to assess the cardiovascular effects consequent to chronic respiratory ex...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-009-9053-3
更新日期:2009-12-01 00:00:00
abstract::Unintentional intravascular administration of bupivacaine may cause local anesthetic systemic toxicity (LAST). Although many systems are affected in LAST, the cardiovascular effects can be life-threatening. Remifentanil is a selective, ultra-short-acting, µ-opioid receptor agonist opioid. This study assessed the effec...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-017-9413-3
更新日期:2018-02-01 00:00:00
abstract::Oxidative stress plays an important role in the pathogenesis of hypertension. Epigallocatechin-3-O-gallate (EGCG) is the main polyphenol present in green tea and is known for its potent antioxidant and anti-inflammatory properties. In the present study, we hypothesize that EGCG attenuates oxidative stress in the parav...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-015-9335-x
更新日期:2016-07-01 00:00:00
abstract::Doxorubicin (Dox), an antitumor anthracycline antibiotic, plays a key role in the treatment of many neoplastic diseases. However, its chronic administration induces cardiomyopathy. Increased oxidative stress is a major factor implicated in Dox-induced cardiotoxicity. We hypothesized that a pre-treatment with grape see...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-012-9155-1
更新日期:2012-06-01 00:00:00
abstract::Lithium is one of the classical drugs that have been widely used for treating bipolar disorder. However, several cardiac side effects including sick sinus syndrome, bundle branch block, ventricular tachycardia/fibrillation, non-specific T-wave abnormalities in addition to Brugada-type electrocardiographic changes have...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-018-9464-0
更新日期:2018-12-01 00:00:00
abstract::The ubiquitous nature of plastics has raised concerns pertaining to continuous exposure to plastic polymers and human health risks. Of particular concern is the use of endocrine-disrupting chemicals in plastic production, including di(2-ethylhexyl)phthalate (DEHP) and bisphenol A (BPA). Widespread and continuous expos...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-014-9258-y
更新日期:2014-12-01 00:00:00
abstract::Scientific and medical evidence over the past 30 years has established striking parallels between atherosclerosis and cancer--pathogenetic relationships that cross the boundaries of fiction into the realm of reason. Both diseases in humans are characterized by uncontrolled regulation of cellular growth and differentia...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1385/ct:5:3:245
更新日期:2005-01-01 00:00:00
abstract::DLBS1425 is a bioactive compound extracted from Phaleria macrocarpa, with anti-proliferative, anti-inflammatory and anti-angiogenic properties against cancer cells. The present study was aimed to assess cardiotoxicity of DLBS1425, compared to the mainstay regimen for breast cancer, 5-fluorouracil:doxorubicin:cyclophos...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-014-9275-x
更新日期:2015-01-01 00:00:00
abstract::We investigated protective effect of FK506 on rat hearts subjected to ischemia/reperfusion (I/R) injury by regulating CaN and ASK1. Wistar rats were divided into four groups: Ischemia/reperfusion group (I/R), FK506 + Ischemia/reperfusion group (FK506-I/R), sham group, and FK506 + sham group (FK506-sham). Ischemia/repe...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-010-9095-6
更新日期:2011-03-01 00:00:00
abstract::3,4-Methylenedioxymethamphetamine (MDMA), more commonly known as "ecstasy," is a semi-synthetic entactogenic phenylethylamine. In recent years it has gained popularity as a recreational drug whose use has registered an upward trend especially among adolescents and young adults. Despite its unwarranted reputation of be...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-019-09526-9
更新日期:2019-12-01 00:00:00
abstract::The purpose of this essay is to overview our findings that membrane-associated calcium-independent phospholipase A2 is markedly inhibited by low, clinically relevant concentrations of anthracyclines. Our studies suggest that due to the essential role of this enzyme in membrane homeostasis, its inhibition can be one of...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-007-0012-6
更新日期:2007-01-01 00:00:00
abstract::Cerium dioxide nanoparticles (CeO2 NPs) are an engineered nanomaterial (ENM) that possesses unique catalytic, oxidative, and reductive properties. Currently, CeO2 NPs are being used as a fuel catalyst but these properties are also utilized in the development of potential drug treatments for radiation and stroke protec...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-013-9213-3
更新日期:2013-12-01 00:00:00
abstract::Congestive heart failure (CHF) afflicts over 5 million individuals in the United States, and most die within 5 yr of diagnosis. Because of the high morbidity and mortality associated with CHF, the search for biomarkers to predict, diagnose, and manage this disease has intensified. Calcium homeostasis and alterations i...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1385/ct:1:2:165
更新日期:2001-01-01 00:00:00
abstract::This study was aimed at determining the efficacy of epinephrine, followed by chest compressions, in producing a return of spontaneous circulation (ROSC) during cyanide (CN)- or hydrogen sulfide (H2S)-induced toxic cardiac pulseless electrical activity (PEA) in the rat. Thirty-nine anesthetized rats were exposed to eit...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-018-9454-2
更新日期:2018-10-01 00:00:00
abstract::Cardiac toxicity has been associated with HIV infection and exposure to nucleoside reverse transcriptase inhibitors (NRTIs), but the role of the latter in the development of cardiac disease of HIV-infected patients is uncertain. To investigate the cardiotoxicity of transplacentally administered zidovudine (AZT) or AZT...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1385/ct:4:2:133
更新日期:2004-01-01 00:00:00
abstract::Asiatic acid (AA), a pentacyclic triterpenoid, is a key phytoconstituent of Centella asiatica. AA is a patented as a cytotoxic substance, and it exerts cytotoxicity against Hep3B, Hela and MCF-7 cell lines. However, pentacyclic triterpenoids also modulate the expression and transcriptional activities of Nrf2 and exert...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-017-9424-0
更新日期:2018-04-01 00:00:00
abstract::The effects of magnesium (Mg2+) on ischaemic complications of pathological cardiac hypertrophy are unclear. In this study, we investigated effects of Mg2+ pretreatment on ischaemia/reperfusion (I/R) injury in isoprenaline (ISO)-induced hypertrophic hearts. Wistar rats were treated for 7 days with different combination...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-015-9355-6
更新日期:2017-01-01 00:00:00
abstract::We have compared the cardiotoxicity of five platinum complexes in a model of isolated rat heart using the Langendorff technique. These effects were assessed via coronary flow (CF) and cardiac functional parameters. cis-Diamminedichloroplatinum(II) (cisplatin, CDDP), dichloro-(1,2-diaminocyclohexane)platinum(II) (Pt((I...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-014-9293-8
更新日期:2015-07-01 00:00:00
abstract::Dofetilide is a class III antiarrhythmic used for treating atrial dysrhythmias. Though its adverse effects are well described in routine use, very little is known about dofetilide toxicity in overdose. This is a retrospective case series of consecutive patients reported to our poison center after dofetilide overdose. ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-016-9384-9
更新日期:2017-07-01 00:00:00
abstract::Serotonergic drugs may lead to valvular heart disease in humans and more recently also in rats. Although clinical data suggest that dose dependency and reversibility after drug cessation might occur, proof of this is lacking. For that purpose, a total of 106 rats were prospectively enrolled: 22 control animals and 7 g...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-009-9046-2
更新日期:2009-09-01 00:00:00
abstract::Epidemiological studies have associated air pollution particulate matter (PM) exposure with adverse cardiovascular effects. Identification of causal PM sources is critically needed to support regulatory decisions to protect public health. This research examines the in vitro cardiotoxicity of bioavailable constituents ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-013-9225-z
更新日期:2013-12-01 00:00:00
abstract::Erythromycin is an antibiotic that prolongs the QT-interval and causes Torsade de Pointes (TdP) by blocking the rapid delayed rectifying potassium current (IKr) without affecting either the slow delayed rectifying potassium current (IKs) or inward rectifying potassium current (IK1). Erythromycin exerts this effect in ...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-019-09532-x
更新日期:2019-12-01 00:00:00
abstract::Our aim is to investigate the correlation between 9p21 chromosome rs4977574 polymorphism genotypes and the development of coronary artery heart disease (CHD). Two hundred and eighty-nine patients with angiography-confirmed CHD were recruited as the CHD group, while 338 subjects without CHD symptoms were enrolled as th...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-016-9372-0
更新日期:2017-04-01 00:00:00
abstract::Trichloroethylene is an organic solvent used as an industrial degreasing agent. Due to its widespread use and volatile nature, TCE is a common environmental contaminant. Trichloroethylene exposure has been implicated in the etiology of heart defects in human populations and animal models. Recent data suggest misregula...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章
doi:10.1007/s12012-010-9066-y
更新日期:2010-06-01 00:00:00
abstract::Nucleoside reverse transcriptase inhibitor (NRTI)-induced cardiomyopathy has been suggested to reflect mitochondrial targets of drug toxicity. The prevailing hypothesis is that, through structural mimicry, the NRTIs are mistaken as substrates for DNA polymerase and incorporated into replicating DNA, where they cause t...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1385/ct:4:3:217
更新日期:2004-01-01 00:00:00
abstract::Arsenic trioxide (ATO) is among the first-line chemotherapeutic drugs used in oncological practice. It has shown substantial efficacy in treating patients with relapsed or refractory acute promyelocytic leukaemia. The clinical use of ATO is hampered due to cardiotoxicity and hence many patients are precluded from rece...
journal_title:Cardiovascular toxicology
pub_type: 杂志文章,评审
doi:10.1007/s12012-018-09504-7
更新日期:2019-04-01 00:00:00