Extremely low frequency magnetic fields affect transposition activity in Escherichia coli.

Abstract:

:The aim of this study was to verify whether extremely low frequency (ELF) magnetic fields (MF) could affect transposition activity like some environmental stress factors such as heat shock or UV irradiation. Using an Escherichia coli Lac Z(-) strain transformed with a plasmid containing a Tn 10 derivative element expressing beta-galactosidase only after transposition, it was possible to determine the events of transposition evaluating the rate at which the colonies developed dark coloured papillae (Lac Z(+)). We found that those bacteria that had been exposed for a long time (58 h) to a 50 Hz low intensity MF (0.1-1 mT) gave colonies with significantly lower transposition activity compared to sham-exposed bacteria. Such reduction in transposition activity was positively correlated to the intensity of the MF, in a dose-effect manner. This phenomenon was not affected by bacterial cell proliferation, since no significant differences were observed in number, diameter and perimeter between sham-exposed and MF-exposed colonies.

journal_name

Radiat Environ Biophys

authors

Del Re B,Garoia F,Mesirca P,Agostini C,Bersani F,Giorgi G

doi

10.1007/s00411-003-0192-9

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

113-8

issue

2

eissn

0301-634X

issn

1432-2099

journal_volume

42

pub_type

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