Abstract:
:Ionizing radiation can produce adverse biological effects in astronauts during space travel. Of particular concern are the types of radiation from highly energetic, heavy, charged particles known as HZE particles. The aims of our studies are to characterize HZE particle radiation induced biological effects and evaluate the effects of L-selenomethionine (SeM) on these adverse biological effects. In this study, microarray technology was used to measure HZE radiation induced changes in gene expression, as well as to evaluate modulation of these changes by SeM. Human thyroid epithelial cells (HTori-3) were irradiated (1 GeV/n iron ions) in the presence or in the absence of 5 microM SeM. At 6 h post-irradiation, all cells were harvested for RNA isolation. Gene Chip U133Av2 from Affymetrix was used for the analysis of gene expression, and ANOVA and EASE were used for a determination of the genes and biological processes whose differential expression is statistically significant. Results of this microarray study indicate that exposure to small doses of radiation from HZE particles, 10 and 20 cGy from iron ions, induces statistically significant differential expression of 196 and 610 genes, respectively. In the presence of SeM, differential expression of 77 out of 196 genes (exposure to 10 cGy) and 336 out of 610 genes (exposure to 20 cGy) is abolished. In the presence or in the absence of SeM, radiation from HZE particles induces differential expression of genes whose products have roles in the induction of G1/S arrest during the mitotic cell cycle, as well as heat shock proteins. Some of the genes, whose expressions were affected by radiation from HZE particles and were unchanged in irradiated cells treated with SeM, have been shown to have altered expression levels in cancer cells. The conclusions of this report are that radiation from HZE particles can induce differential expression of many genes, some of which are known to play roles in the same processes that have been shown to be activated in cells exposed to radiation from photons (like cell cycle arrest in G1/S), and that supplementation with SeM abolishes HZE particle-induced differential expression of many genes. Understanding the roles that these genes play in the radiation-induced transformation of cells may help to decipher the origins of radiation-induced cancer.
journal_name
Radiat Environ Biophysjournal_title
Radiation and environmental biophysicsauthors
Stewart J,Ko YH,Kennedy ARdoi
10.1007/s00411-006-0089-5subject
Has Abstractpub_date
2007-06-01 00:00:00pages
161-5issue
2eissn
0301-634Xissn
1432-2099journal_volume
46pub_type
杂志文章abstract::The influence of microgravity on the repair of x-ray-induced DNA double-strand breaks was studied in the temperature-conditional repair mutant rad54-3 of diploid yeast Saccharomyces cerevisiae. Cells were exposed on the ground and kept at a low temperature until microgravity conditions were achieved. In orbit, they we...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050149
更新日期:1999-07-01 00:00:00
abstract::The relationship between occupational exposure to extremely low-frequency electromagnetic fields and adult leukaemia has been studied extensively during the last decade. The first studies were based on crude exposure assessments, estimated through job titles, with no or only limited control confounding factors. The re...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s004110050036
更新日期:1996-11-01 00:00:00
abstract::Irradiation of human lymphocytes by alpha-particles under different conditions has been seen to be substantially more effective in the induction of dicentric chromosomes than irradiation by gamma-rays. However, the relative biological effectiveness (RBE) determined in these studies differed by a factor of more than 10...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050028
更新日期:1996-08-01 00:00:00
abstract::More accurate reconstruction of the radioactive contamination of the Techa River system in 1949-1951 has been made on the basis of refined data on the amounts and the rate of discharge of radionuclides into the Techa River from the Mayak Production Association; this has led to the development of a modified Techa River...
journal_title:Radiation and environmental biophysics
pub_type: 历史文章,杂志文章
doi:10.1007/s00411-012-0414-0
更新日期:2012-11-01 00:00:00
abstract::Quantitative cancer incidence data exist for various laboratory animal models, but little of this information is usable for estimating human risks, primarily because of uncertainties about possible mechanistic differences among species. Acceptance and utilization of animal data for human risk assessment will require a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0106-3
更新日期:2007-06-01 00:00:00
abstract::For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are relatively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tiss...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-011-0371-z
更新日期:2011-08-01 00:00:00
abstract::Various transport models are presently used to predict the long-term migration behaviour of fallout radiocesium on the soil. To examine to what extent the uncertainty of these predictions is influenced by the spatial variability of the migration rates, we determined the depth profiles of Chernobyl-derived 137Cs at 100...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110000062
更新日期:2000-09-01 00:00:00
abstract::Ehrlich ascites tumour cells were irradiated with negative pions at different positions in the plateau and the peak of the depth absorbed dose curve. Dose-survival curves for immediate testing of the viability of the cells are given and are compared with other types of radiations in use for therapy. From the data obta...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01358286
更新日期:1979-08-10 00:00:00
abstract::Radiation is known to be carcinogenic to humans but attempts to demonstrate the process using human tissue culture models have met with little success. In the present study explants were established from urothelium and exposed to radiation and a range of chemical carcinogens, suspected promotor or metabolic agents. Th...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01210524
更新日期:1990-01-01 00:00:00
abstract::The TEL1 gene from Saccharomyces cerevisiae has been shown to be the closest sequence homologue to ATM, the gene mutated in ataxia-telangiectasia (A-T) patients. Functional homology shared between the ATM and Tell proteins has recently been demonstrated based on heterologous expression of the TEL1 gene in human cells ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-001-0125-4
更新日期:2001-12-01 00:00:00
abstract::Data on rats exposed to cigarette smoke before or after exposure to radon are used to estimate smoke-dependent parameters of the biologically based two-stage clonal expansion model. The baseline parameters and the action of radon acting on initiation and promotion were fixed based on earlier work. Cigarette smoke acti...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-005-0006-3
更新日期:2005-10-01 00:00:00
abstract::Roots of Pisum sativum L. were chronically exposed to 60-Hz vertical electric fields ranging from 150 to 450V/m in an aqueous medium whose conductivity was approximately 0.07 S/m. Control and exposed roots were grown concomitantly in the same tank whose medium was continuously circulated and maintained at 19 degrees C...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323679
更新日期:1983-01-01 00:00:00
abstract::The results of a study in which groups of sheep were given single oral administrations of 14C, 3H and 35S and then slaughtered over a period of 1 year are reported. The experimental data were used to investigate the potential of metabolically based models for describing the transfer of the three radionuclides to sheep...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050078
更新日期:1998-02-01 00:00:00
abstract::In the process of developing a new dosimetry system for atomic bomb survivors in Hiroshima and Nagasaki (DS02), an intercomparison study between (152)Eu and (36)Cl measurements was proposed, to reconcile the discrepancy previously observed in the Hiroshima data between measurements and calculations of thermal neutron ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-008-0166-z
更新日期:2008-07-01 00:00:00
abstract::Dose conversion coefficients (DCCs) for assessment of internal and external radiation exposures to terrestrial and aquatic biota are compiled for 75 radionuclides, for 14 terrestrial and 22 aquatic reference organisms. DCC values for internal exposure are calculated based on a homogeneous distribution of the radionucl...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-008-0159-y
更新日期:2008-04-01 00:00:00
abstract::The method of iteratively reweighted least squares for the regression analysis of Poisson distributed chromosome aberration data is reviewed in the context of other fit procedures used in the cytogenetic literature. As an application of the resulting regression curves methods for calculating confidence intervals on do...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323412
更新日期:1983-01-01 00:00:00
abstract::Two data groups were analyzed: (1) the exposure rate in the former Czechoslovakia after the Chernobyl accident in 1986, and (2) the decrease of beta activity of an atmospheric fallout sample taken in Bratislava during 24 h on 30 May 1965. Both quantities decreased with the first power of time. This pattern of decrease...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-005-0276-9
更新日期:2005-05-01 00:00:00
abstract::A Monte Carlo model is presented to study details of the energy deposition inside tracks of heavy charged particles in water vapor. The input data for most of the calculations based on the binary encounter approximation are double-differential cross sections for electron emission after heavy-ion impact. The paths of t...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF01219334
更新日期:1994-01-01 00:00:00
abstract::Growing hair follicles with their rapid cell proliferation would be expected to be sensitive organs to cytotoxic agents such as radiation. Various abnormalities in the hair and hair follicles have been reported in the past. Changes in the number of cells in the newly forming hair cortex have been shown in the mouse to...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF02434035
更新日期:1996-05-01 00:00:00
abstract::Checkpoints are components of signalling pathways involved in genome stability. We analysed the putative dual functions of Rad17 and Chk1 as checkpoints and in DNA repair using mutant strains of Saccharomyces cerevisiae. Logarithmic populations of the diploid checkpoint-deficient mutants, chk1Delta/chk1Delta and rad17...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0119-y
更新日期:2007-11-01 00:00:00
abstract::In this study, temporal variations of indoor radon concentrations in Bulgaria were investigated. The radon concentrations were measured by nuclear track detectors as part of the Bulgarian National Survey, performed in the dwellings of 28 regional districts. The detectors were exposed through a year in two consecutive ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-019-00789-y
更新日期:2019-08-01 00:00:00
abstract::Osteosarcoma is the most frequent secondary malignancy following radiotherapy of patients with bilateral retinoblastoma. This suggests that the Rb1 tumour suppressor gene might confer genetic susceptibility towards radiation-induced osteosarcoma. To define the contribution of the Rb1 pathway in the multistep process o...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0339-4
更新日期:2011-03-01 00:00:00
abstract::One of the main purposes leading botanists to investigate the effects of ionizing radiations is to understand plant behaviour in space, where vegetal systems play an important role for nourishment, psychological support and functioning of life support systems. Ground-based experiments have been performed with particle...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s00411-010-0343-8
更新日期:2011-03-01 00:00:00
abstract::In spite of a well-known relationship between exposure to radiation and increased risk for cancer development, the biological mechanisms involved in radiation-induced carcinogenesis remain poorly documented. Various hypotheses are discussed in this paper. It appears that radiation cannot be directly responsible for th...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/BF01209747
更新日期:1995-11-01 00:00:00
abstract::The effect of gamma radiation on red blood cells have been examined using a spin labeling method. For this purpose two spin labels were used to monitor membrane fluidity: methyl 5-doxylpalmitate (Met 5-DP) and methyl 12-doxylstearate (Met 12-DS). The irradiation of red cells with the doses of 200 and 500 Gy caused dec...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01595573
更新日期:1991-01-01 00:00:00
abstract::High steady-state transcriptional activity is essential for normal mitochondrial function. The requisite transcription rate is satisfied in part by high copy number of mitochondrial DNA (mtDNA). In the present study, we analyze mtDNA copy number by real-time PCR in nucleated blood cells from control mice and mice expo...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0329-6
更新日期:2011-03-01 00:00:00
abstract::Working from the general postulate that natural selection of plant form operates so as to maximize the survival potential of a species, this paper examines the hypothesis that the mechanical support of tree foliage must approach optimality in the use of wood, i.e., that tree stems and branches will have "optimal form"...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323263
更新日期:1978-08-10 00:00:00
abstract::In this work, direct DNA damage induced by low-energy electrons (sub-keV) is simulated using a Monte Carlo method. The characteristics of the present simulation are to consider the new mechanism of DNA damage due to dissociative electron attachment (DEA) and to allow determining damage to specific bases (i.e., adenine...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-016-0681-2
更新日期:2017-03-01 00:00:00
abstract::A new device for the measurement of complete laser induced fluorescence emission spectra (maxima near 690 and 735 nm) of leaves during the induction of the chlorophyll fluorescence is described. In this the excitation light (cw He/Ne laser, 632.8 nm) is switched on by a fast electro-mechanical shutter which provides a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211213
更新日期:1992-01-01 00:00:00
abstract::The Sax-Markov binary eurejoining/misrejoining (SMBE) model is a stochastic representation of Sax's breakage-and-reunion mechanism of misrejoining DNA double-strand breaks (DSBs). In this model, to approximate DSB misrejoining probabilities that decrease with increasing distance, the nucleus is treated as a collection...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110000067
更新日期:2000-12-01 00:00:00