Abstract:
:High steady-state transcriptional activity is essential for normal mitochondrial function. The requisite transcription rate is satisfied in part by high copy number of mitochondrial DNA (mtDNA). In the present study, we analyze mtDNA copy number by real-time PCR in nucleated blood cells from control mice and mice exposed to 1- or 10-Gy X-radiation. Transcription of the oxidative phosphorylation-associated genes cytb, atp6, nd4, nd2 and d-loop region was monitored in these nucleated blood cells similarly by real-time PCR. We observed a 50% decrease in the ratio of mitochondrial to nuclear DNA (mtDNA/nDNA) in blood cells, while the mtDNA/nDNA ratio in serum increased. After a lethal 10-Gy dose of X-irradiation, we observed an 80% decrease in the number of circulating lymphocytes. In response to a 10-Gy radiation dose, we observed transiently increased mtDNA/nDNA ratio and transcription within the initial 5 h post-treatment. At 24-72 h, the mtDNA/nDNA ratio in surviving cells was reduced to the level observed in blood cells irradiated with 1 Gy. We observed a decrease in the serum mtDNA/nDNA ratio due to an increase in nDNA content rather than a decrease in mtDNA. Taken together, results presented herein suggest that the mtDNA/nDNA ratio may be of clinical value potentially as a diagnostic tool, particularly in oncology patients undergoing radiation therapy.
journal_name
Radiat Environ Biophysjournal_title
Radiation and environmental biophysicsauthors
Evdokimovsky EV,Ushakova TE,Kudriavtcev AA,Gaziev AIdoi
10.1007/s00411-010-0329-6subject
Has Abstractpub_date
2011-03-01 00:00:00pages
181-8issue
1eissn
0301-634Xissn
1432-2099journal_volume
50pub_type
杂志文章abstract::As a consequence of the increased use of renewable energy and the now long distances between energy generation and consumption, in Europe, electric power transfer by high-voltage (HV) direct current (DC) overhead power lines gains increasing importance. Thousands of kilometers of them are going to be built within the ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-014-0520-2
更新日期:2014-05-01 00:00:00
abstract::The effect of gamma radiation on red blood cells have been examined using a spin labeling method. For this purpose two spin labels were used to monitor membrane fluidity: methyl 5-doxylpalmitate (Met 5-DP) and methyl 12-doxylstearate (Met 12-DS). The irradiation of red cells with the doses of 200 and 500 Gy caused dec...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01595573
更新日期:1991-01-01 00:00:00
abstract::The effect of ionizing irradiation on the physiological activity of cyclic adenosine monophosphate (cAMP) in smooth muscle preparations from frog lung was studied. cAMP, given as dibutyryl salt (dib-cAMP) inhibited the radiation induced contractions of the muscle in a manner similar to the action of theophylline. In v...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323750
更新日期:1982-01-01 00:00:00
abstract::Recent analyses of the Canadian fluoroscopy cohort study reported significantly increased radiation risks of mortality from ischemic heart diseases (IHD) with a linear dose-response adjusted for dose fractionation. This cohort includes 63,707 tuberculosis patients from Canada who were exposed to low-to-moderate dose f...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-019-00819-9
更新日期:2020-03-01 00:00:00
abstract::A temporary increase in the incidence of infant leukaemia in Greece was reported by Petridou et al., which was attributed to in utero exposure to ionising radiation resulting from the Chernobyl accident. We performed a similar analysis based on the data of the German Childhood Cancer Registry in order to check whether...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050099
更新日期:1998-07-01 00:00:00
abstract::To explore the expression level and the role of peroxisome proliferator-activated receptor gamma (PPAR-γ) in radiation-induced heart injury in a rat model, thirty-two Sprague-Dawley rats were divided into three groups (the control group, the 15-Gy irradiation group and the 18-Gy irradiation group). Experimental animal...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-011-0390-9
更新日期:2012-03-01 00:00:00
abstract::Many experimental studies are carried out to compare biological effectiveness of high dose rate (HDR) with that of low dose rate (LDR). The rational for this is the uncertainty regarding the value of the dose rate effectiveness factor (DREF) used in radiological protection. While a LDR is defined as 0.1 mGy/min or low...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-020-00852-z
更新日期:2020-08-01 00:00:00
abstract::The ion microprobe SNAKE at the Munich 14 MV tandem accelerator achieves beam focussing by a superconducting quadrupole doublet and can make use of a broad range of ions and ion energies, from 20 MeV protons to 200 MeV gold ions. Because of these properties, SNAKE is particularly attractive for biological microbeam ex...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-003-0222-7
更新日期:2004-02-01 00:00:00
abstract::Non-linear mechanical response of viscoelastic strain responding media to high amplitude stress-strain is examined from a phenomenological point of view and found to lead to results compatible with empirical observations of high intensity ultrasound irradiation of brain, liver, and eye lens tissues. The proposed hyste...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01324230
更新日期:1981-01-01 00:00:00
abstract::The kinetics of fluoresceindiacetate (FDA)-hydrolysis in erythrocytes, CHO-cells and homogenized cells was investigated by means of fluorescence spectroscopy. At a 10(-6)m FDA concentration the enzymatic conversion in the homogenized cells can be described by first order reactions. When studying intact cells, total ki...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01331024
更新日期:1977-04-27 00:00:00
abstract::125I incorporated in DNA is known to be exceptionally toxic. Values of D0 range from about 40 to about 90 decays for survival of mammalian cells. The effectiveness of 125I in DNA with respect to the induction of breaks of the DNA strands, however, appears to be comparatively low. The numbers of strand breaks per energ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01213705
更新日期:1987-01-01 00:00:00
abstract::Age-specific and cumulative mortality rates are presented for different cancer sites from 1970 until 1988 for the 11 individual federal states of West Germany (FRG). Sex- and age-specific evaluations are performed and temporal and regional trends in mortality from different cancer sites are revealed. In the FRG there ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01212802
更新日期:1993-01-01 00:00:00
abstract::Since the early 1990s, information on radiation-exposed populations other than those exposed from the Chernobyl accident in 1986 has become increasingly available for international scientific research. It is essential to understand how the cohorts of exposed populations have been defined and what mechanisms can be use...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s00411-001-0128-1
更新日期:2002-03-01 00:00:00
abstract::Preliminary results on the spectral dependence of the absorption of light in tissue are presented and discussed with respect to medical laser application. ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323942
更新日期:1977-10-12 00:00:00
abstract::A new device for the measurement of complete laser induced fluorescence emission spectra (maxima near 690 and 735 nm) of leaves during the induction of the chlorophyll fluorescence is described. In this the excitation light (cw He/Ne laser, 632.8 nm) is switched on by a fast electro-mechanical shutter which provides a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211213
更新日期:1992-01-01 00:00:00
abstract::The main target of this work is to examine blood clearance and external exposure for 177Lu-DOTATATE compared with new emerging 177Lu-PSMA therapy. Blood clearance and radiation exposure of 31 patients treated with 5.5 ± 1.1 GBq 177Lu-DOTATATE were compared to those of 23 patients treated with 7.4 GBq 177Lu-PSMA. Dose ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-017-0721-6
更新日期:2018-03-01 00:00:00
abstract::About a century ago, Conrad Röentgen discovered X-rays, and Henri Becquerel discovered a new phenomenon, which Marie and Pierre Curie later coined as radio-activity. Since their seminal work, we have learned much about the physical properties of radiation and its effects on living matter. Alas, the more we discover, t...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s00411-007-0146-8
更新日期:2008-02-01 00:00:00
abstract::Exposure to ionizing radiation has well-documented long-term effects on cancer rates and other health outcomes in humans. While in vitro experimental studies had demonstrated that the nature of some radiation effects depend on both total dose of the radiation and the dose rate (i.e., the pattern of dose distribution o...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-016-0670-5
更新日期:2017-03-01 00:00:00
abstract::Blue (F450) and green (F530) leaf fluorescence were studied together with the red chlorophyll fluorescence (emission maxima F690 and F735) during light-induced chlorophyll fluorescence induction kinetics (Kautsky effect) in predarkened leaves of wheat (Triticum aestivum L.) and soybean (Glycine max L.). The intensity ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01225923
更新日期:1993-01-01 00:00:00
abstract::The rare earth radionuclides 177 Lu and 153Sm were administered as single i.p. injections in NMRI mice. Lu was deposited principally (up to 60%) in the skeleton if the quantity of stable carrier was low. Increase of stable carrier enhanced deposition in the reticulo-endothelial system. Sm was preferentially deposited ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01324368
更新日期:1980-01-01 00:00:00
abstract::This article describes the application of a simplified Bayesian method for estimation of doses from a mixed field using cytogenetic biological dosimetry, taking as an example neutron and gamma radiation emitted from the MARIA nuclear research reactor in Poland. The Bayesian approach is a good alternative to the common...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-018-0764-3
更新日期:2019-03-01 00:00:00
abstract::Investigation of cell-free DNA (cf-DNA) in body fluids, as a potential biomarker for assessing the effect of ionizing radiation on the organism, is of considerable interest. We investigated changes in the contents of cell-free mitochondrial DNA (cf-mtDNA) and cell-free nuclear DNA (cf-nDNA) in the urine of X-ray-expos...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-015-0599-0
更新日期:2015-08-01 00:00:00
abstract::Testing the limits of survivability in space is the primary focus in astrobiological research. Although a number of previous studies have examined terrestrial life survival in an extraterrestrial environment, only a few have investigated how life systems respond to high doses of alpha cosmic ray, the main component of...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-019-00803-3
更新日期:2019-08-01 00:00:00
abstract::The aim of this study was to verify whether extremely low frequency (ELF) magnetic fields (MF) could affect transposition activity like some environmental stress factors such as heat shock or UV irradiation. Using an Escherichia coli Lac Z(-) strain transformed with a plasmid containing a Tn 10 derivative element expr...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-003-0192-9
更新日期:2003-07-01 00:00:00
abstract::With a model system of pBR322 plasmid DNA solution in vitro, the dose effects of radiation-induced single- and double-strand breaks (SSB and DSB) were measured and DSB was distinguished into alpha- and beta-types. Under the condition of low scavenging capacity existing in the irradiated DNA solution, SSB and alpha DSB...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110000054
更新日期:2000-06-01 00:00:00
abstract::137Cs from global fallout of nuclear weapon testings in the 1950s and 1960s was determined in successive layers (0-30 cm) of eight undisturbed grassland soils in Bavaria, Germany. The maximum activity concentration was found in soil layers between 4 and 15 cm below the surface. Using the vertical distribution of the c...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050088
更新日期:1998-04-01 00:00:00
abstract::At five stages of gestation, mice were exposed dorsally to ultrasonic irradiation at 1 W/cm2 (spatial and temporal average), 2 MHz, and 34 and 37 degrees C (temperature at beginning of exposure). The results indicated that ultrasonic exposure up to at least 100 s is not hazardous to a pregnant mouse or its offspring i...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323651
更新日期:1980-01-01 00:00:00
abstract::In the process of developing a new dosimetry system for atomic bomb survivors in Hiroshima and Nagasaki (DS02), an intercomparison study between (152)Eu and (36)Cl measurements was proposed, to reconcile the discrepancy previously observed in the Hiroshima data between measurements and calculations of thermal neutron ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-008-0166-z
更新日期:2008-07-01 00:00:00
abstract::Because of the increasing application of ionizing radiation in medicine, quantitative data on effects of low-dose radiation are needed to optimize radiation protection, particularly with respect to cataract development. Using mice as mammalian animal model, we applied a single dose of 0, 0.063, 0.125 and 0.5 Gy at 10 ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-017-0728-z
更新日期:2018-05-01 00:00:00
abstract::This study was undertaken to investigate the mechanism of chemical radiosensitization by halogenated bases incorporated into DNA. Radiation-induced base and sugar-phosphate backbone damage to 5-bromouridine-5'-monophosphate (5-BrUMP) was monitored using a flow system connected in series with a recording spectrophotome...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211508
更新日期:1992-01-01 00:00:00