Confocal microscopy for modeling electron microbeam irradiation of skin.

Abstract:

:For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are relatively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tissues. Employing high-resolution confocal microscopy, we have determined the spatial distribution, size, and shape of epidermal keratinocyte nuclei for the full-thickness EpiDerm™ skin model (MatTek, Ashland, VA). Application of these data to calculate the microdosimetry and microdistribution of energy deposition by an electron microbeam is discussed.

journal_name

Radiat Environ Biophys

authors

Miller JH,Chrisler WB,Wang X,Sowa MB

doi

10.1007/s00411-011-0371-z

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

365-9

issue

3

eissn

0301-634X

issn

1432-2099

journal_volume

50

pub_type

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