Ionizing radiation-induced structural modification of human red blood cells.

Abstract:

:The effect of gamma radiation on red blood cells have been examined using a spin labeling method. For this purpose two spin labels were used to monitor membrane fluidity: methyl 5-doxylpalmitate (Met 5-DP) and methyl 12-doxylstearate (Met 12-DS). The irradiation of red cells with the doses of 200 and 500 Gy caused decrease of microviscosity in certain regions of lipid bilayer (as indicated by Met 5-DP and Met 12-DS spectra) but did not affect lipid order parameter. The behavior of two other spin labels, maleimide(4-maleimido-2,2,6,6-tetramethylpiperidine-1-oxyl) and TEMPONE (4-oxo-2,2,6,6-tetramethylpiperidine-1-oxyl) indicated: 1) conformational changes of membrane proteins, 2) modification of cell internal peptides and proteins, 3) decreased internal viscosity of red blood cells.

journal_name

Radiat Environ Biophys

authors

Gwoździński K

doi

10.1007/BF01595573

subject

Has Abstract

pub_date

1991-01-01 00:00:00

pages

45-52

issue

1

eissn

0301-634X

issn

1432-2099

journal_volume

30

pub_type

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