Abstract:
:The fluorescent intercalation complex of ethidium bromide with DNA was used as a probe to demonstrate damage in the base-pair region of DNA, due to the action of superoxide radicals. The O.2- radical itself, generated by gamma-radiolysis of oxygenated aqueous Na-formate solutions, is rather ineffective with respect to impairment of DNA. Copper(II) ions, known to interact with DNA by coordinate binding at purines, enhance the damaging effect of O.2-. Addition of H2O2 to the DNA/Cu(II) system gives rise to further enhancement, so that DNA impairment by O.2- becomes comparable to that initiated by .OH radicals. These results suggest that the modified, Cu(II)-catalysed, Haber-Weiss process transforms O.2- into .OH radicals directly at the target molecule, DNA-Cu2+ + O.2-----DNA-Cu+ + O2 DNA-Cu+ + H2O2----DNA...OH + Cu2+ + OH- in a "site-specific" mechanism as proposed for other systems (Samuni et al. 1981; Aronovitch et al. 1984). Slow DNA decomposition also occurs without gamma-irradiation by autocatalysis of DNA/Cu(II)/H2O2 systems. In this context we observed that Cu(II) in the DNA-Cu2+ complex (unlike free Cu2+) is capable of oxidizing Fe(II) to Fe(III), thus the redox potential of the Cu2+/Cu+ couple appears to be higher than that of the Fe3+/Fe2+ couple when the ions are complexed with DNA. Metal-catalysed DNA damage by O.2- also occurs with Fe(III), but not with Ag(I) or Cd(II) ions. It was also observed that Cu(II) ions (but neither Ag(I) nor Cd(II] efficiently quench the fluorescence of the intercalation complex of ethidium bromide with DNA.
journal_name
Radiat Environ Biophysjournal_title
Radiation and environmental biophysicsauthors
Prütz WAdoi
10.1007/BF01326732subject
Has Abstractpub_date
1984-01-01 00:00:00pages
7-18issue
1eissn
0301-634Xissn
1432-2099journal_volume
23pub_type
杂志文章abstract::The major goal of this study was to identify and quantitatively describe the association between the characteristics of chronic (low-dose rate) exposure to (low LET) ionizing radiation and cellularity of peripheral blood cell lines. About 3,200 hemograms (i.e., spectra of blood counts) obtained over the years of maxim...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0269-1
更新日期:2010-05-01 00:00:00
abstract::The effects of single pulses and multiple pulses of 7 MV electrons on micronuclei (MN) induction in cytokinesis-blocked human peripheral blood lymphocytes (PBLs) were investigated over a wide range of dose rates per pulse (instantaneous dose rate). PBLs were exposed to graded doses of 2, 3, 4, 6, and 8 Gy of single el...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-011-0353-1
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更新日期:2004-12-01 00:00:00
abstract::Sustained pain relief following radon spa therapy in patients suffering from chronic painful diseases has been well described. But still, the underlying mechanisms are not fully understood. We conducted the prospective and explorative RAD-ON01 study which included 103 patients who suffered from chronic painful musculo...
journal_title:Radiation and environmental biophysics
pub_type: 临床试验,杂志文章
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abstract::Microwave irradiation has been shown to decrease the input resistance of snail neurons. In this study, we examined the role of extracellular calcium in triggering the microwave-induced enhancement of membrane conductance. Two sets of experiments were conducted. In the first set, nerve cells were superfused using Ringe...
journal_title:Radiation and environmental biophysics
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doi:10.1007/BF01229821
更新日期:1985-01-01 00:00:00
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doi:10.1007/s00411-001-0128-1
更新日期:2002-03-01 00:00:00
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journal_title:Radiation and environmental biophysics
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doi:10.1007/BF01340573
更新日期:1979-01-01 00:00:00
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01212653
更新日期:1985-01-01 00:00:00
abstract::The antioxidant properties of hydroxycinnamic acid derivatives (HCA) were studied by laser flash photolysis. The transient species with maximum absorption at 360 nm were assigned to the phenoxyl radical of HCA. The SO (4) (.-) induced oxidation of HCA was also investigated. It was shown that the interaction of SO (4) ...
journal_title:Radiation and environmental biophysics
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doi:10.1007/s00411-006-0041-8
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01332171
更新日期:1976-07-30 00:00:00
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
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更新日期:1976-03-30 00:00:00
abstract::The Auger electron emitter 123I was examined in the form of 4-[123I]iodoantipyrine and as [123I]NaI for its effectiveness in killing cells of different sensitivity to photon irradiation. Micronucleus assays showed that 4-[123I]iodoantipyrine is 2-3 times more effective in cell inactivation than [123I]NaI. This can be ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110000083
更新日期:2001-03-01 00:00:00
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211257
更新日期:1989-01-01 00:00:00
abstract::Fluence spectra at several ground distances in Hiroshima and Nagasaki are provided along with associated fluence-to-kerma coefficients from the Dosimetry System 2002 (DS02). Also included are transmission factors for calculating expected responses of in situ sample measurements of neutron activation products such as (...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0120-5
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211542
更新日期:1995-08-01 00:00:00
abstract::It is usually assumed that sparsely ionizing radiation produces randomly distributed DNA breakages. This seems to be supported by the finding that in some DNA fragments single-strand scissions occur uniformly at all nucleotide sites, regardless of sequence. We performed experiments on two DNA fragments of about 300 bp...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01255271
更新日期:1994-01-01 00:00:00
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-006-0039-2
更新日期:2006-05-01 00:00:00
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journal_title:Radiation and environmental biophysics
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doi:10.1007/BF01358292
更新日期:1979-08-10 00:00:00
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doi:10.1007/BF01323651
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journal_title:Radiation and environmental biophysics
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更新日期:2003-10-01 00:00:00
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journal_title:Radiation and environmental biophysics
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doi:10.1007/BF01275215
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
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更新日期:2011-03-01 00:00:00
abstract::To explore possible applications of iodoacetate (IA), a glycolytic inhibitor, in cancer treatment, we screened its cytotoxicity and radioprotective/sensitizing efficacy in three different mammalian cell lines; A549 (human lung carcinoma), MCF7 (human mammary cancer), a non-cancerous CHO (Chinese hamster ovary) cells a...
journal_title:Radiation and environmental biophysics
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更新日期:2017-08-01 00:00:00
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-012-0410-4
更新日期:2012-05-01 00:00:00
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journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s004110050036
更新日期:1996-11-01 00:00:00
abstract::Checkpoints are components of signalling pathways involved in genome stability. We analysed the putative dual functions of Rad17 and Chk1 as checkpoints and in DNA repair using mutant strains of Saccharomyces cerevisiae. Logarithmic populations of the diploid checkpoint-deficient mutants, chk1Delta/chk1Delta and rad17...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0119-y
更新日期:2007-11-01 00:00:00
abstract::Activity catalysing double-strand DNA recombination has been investigated in human tumour cell lines using an in vitro assay in which nuclear extracts from tumour cells are used to catalyse homologous recombination between deletion plasmids. The cell lines investigated showed comparable constitutive levels of recombin...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050056
更新日期:1997-02-01 00:00:00