Abstract:
:Native horseradish peroxidase, as well as its reduced and carboxymethylated form, and the apoenzyme, showed resistance to photodynamic action. Sensitivity to this action was detected only in reduced and carboxymethylated apoenzyme, when the photooxidation of its histidine residues was observed. When analyzing the bulk hydrophobic character (Hf) and the accessibility coefficients (Br) in those amino acid residues which can be subjected to photooxidation in horseradish peroxidase, it was found that all of them are situated in hydrophobic zones with low accessibility coefficients. This could justify the high resistance of this enzyme to photodynamic action. The only exception is tryptophan-117, which has low values of Hf and Br, and therefore its resistance to photodynamic action can only be explained in terms of its location and environment. Tryptophan-117 would be situated in a zone of antiparallel beta-structure, according to Chou and Fasman's predictive method for protein conformation.
journal_name
Radiat Environ Biophysjournal_title
Radiation and environmental biophysicsauthors
Silva E,Barrera Mdoi
10.1007/BF01212653subject
Has Abstractpub_date
1985-01-01 00:00:00pages
57-61issue
1eissn
0301-634Xissn
1432-2099journal_volume
24pub_type
杂志文章abstract::A new device for the measurement of complete laser induced fluorescence emission spectra (maxima near 690 and 735 nm) of leaves during the induction of the chlorophyll fluorescence is described. In this the excitation light (cw He/Ne laser, 632.8 nm) is switched on by a fast electro-mechanical shutter which provides a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211213
更新日期:1992-01-01 00:00:00
abstract::The model of radiocaesium transfer to sheep presented by Galer et al. provides reliable predictions only for sheep of a similar body weight to those used in the development of the model (approximately 30 kg). To extend the applicability of the model, it was necessary to re-parameterise it in terms of activity concentr...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211238
更新日期:1996-02-01 00:00:00
abstract::C57Bl6-lacZ animals were exposed to a range of low dose-rate simulated solar particle event (sSPE) radiation at the NASA-sponsored Research Laboratory (NSRL) at Brookhaven National Laboratory (BNL). Peripheral blood was harvested from animals from 1 to 12 days after total body irradiation (TBI) to quantify the level o...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0291-3
更新日期:2010-08-01 00:00:00
abstract::The main aim of this study was to investigate the dosimetric characteristics of the INTRABEAM ® system in the presence of air gaps between the surface of applicators (APs) and tumor bed. Additionally, the effect of tissue heterogeneities was another focus. Investigating the dosimetric characteristics of the INTRABEAM®...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-020-00835-0
更新日期:2020-05-01 00:00:00
abstract::The hypoxic cytotoxic effect of chlorpromazine towards E. coli B/r was observed to be dependent on temperature of incubation and irradiation. Increasing the temperature of incubation from ambient to 37 degrees C followed by irradiation revealed that the organisms showed radiosensitivity of a magnitude which was two to...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01229816
更新日期:1985-01-01 00:00:00
abstract::The major part of the liquid radioactive waste released by the Mayak Production Association (PA) radiochemical plant into the Techa river occurred in 1949-1951, but there is information on only one single radiochemical analysis of a water sample taken on 24 and 25 September 1951. These data are here used to assess the...
journal_title:Radiation and environmental biophysics
pub_type: 历史文章,杂志文章,评审
doi:10.1007/s00411-003-0182-y
更新日期:2003-04-01 00:00:00
abstract::Quantitative cancer incidence data exist for various laboratory animal models, but little of this information is usable for estimating human risks, primarily because of uncertainties about possible mechanistic differences among species. Acceptance and utilization of animal data for human risk assessment will require a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0106-3
更新日期:2007-06-01 00:00:00
abstract::Since the early 1990s, information on radiation-exposed populations other than those exposed from the Chernobyl accident in 1986 has become increasingly available for international scientific research. It is essential to understand how the cohorts of exposed populations have been defined and what mechanisms can be use...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s00411-001-0128-1
更新日期:2002-03-01 00:00:00
abstract::125I incorporated in DNA is known to be exceptionally toxic. Values of D0 range from about 40 to about 90 decays for survival of mammalian cells. The effectiveness of 125I in DNA with respect to the induction of breaks of the DNA strands, however, appears to be comparatively low. The numbers of strand breaks per energ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01213705
更新日期:1987-01-01 00:00:00
abstract::Osteosarcoma is the most frequent secondary malignancy following radiotherapy of patients with bilateral retinoblastoma. This suggests that the Rb1 tumour suppressor gene might confer genetic susceptibility towards radiation-induced osteosarcoma. To define the contribution of the Rb1 pathway in the multistep process o...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0339-4
更新日期:2011-03-01 00:00:00
abstract::An inter-comparison of five models designed to predict the effect of ionizing radiation on populations of non-human wildlife, performed under the IAEA EMRAS II programme, is presented and discussed. A benchmark scenario 'Population response to chronic irradiation' was developed in which stable generic populations of m...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-012-0430-0
更新日期:2012-11-01 00:00:00
abstract::To explore the expression level and the role of peroxisome proliferator-activated receptor gamma (PPAR-γ) in radiation-induced heart injury in a rat model, thirty-two Sprague-Dawley rats were divided into three groups (the control group, the 15-Gy irradiation group and the 18-Gy irradiation group). Experimental animal...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-011-0390-9
更新日期:2012-03-01 00:00:00
abstract::The potential of proton microchannel radiotherapy to reduce radiation effects in the healthy tissue but to keep tumor control the same as in conventional proton therapy is further elucidated. The microchannels spread on their way to the tumor tissue resulting in different fractions of the healthy tissue covered with d...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-015-0600-y
更新日期:2015-08-01 00:00:00
abstract::After ingestion or inhalation of radionuclides, internal organs of the human body will be exposed to ionising radiation. Current risk estimates of radiation-associated cancer from internal emitters are largely based on extrapolation of risk from high-dose externally exposed groups. Concerns have been expressed that ex...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,meta分析
doi:10.1007/s00411-007-0122-3
更新日期:2007-11-01 00:00:00
abstract::This work describes the radiological assessment of the near-surface Maisiagala radioactive waste repository (Lithuania) over the period 2005-2012, with focus on water pathways and special emphasis on tritium. The study includes an assessment of the effect of post-closure upgrading, the durability of which is greater t...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-013-0459-8
更新日期:2013-05-01 00:00:00
abstract::The study reported in the present paper aimed to evaluate the effective energy (Eeff) of X-rays emitted from the surface of a bare X-ray probe and from different spherical applicators with various diameters, which are widely employed for low kV intraoperative radiotherapy (IORT) of breast cancer. A previously validate...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-020-00887-2
更新日期:2021-01-03 00:00:00
abstract::Because of the increasing application of ionizing radiation in medicine, quantitative data on effects of low-dose radiation are needed to optimize radiation protection, particularly with respect to cataract development. Using mice as mammalian animal model, we applied a single dose of 0, 0.063, 0.125 and 0.5 Gy at 10 ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-017-0728-z
更新日期:2018-05-01 00:00:00
abstract::The oxyhemoglobin saturation (HbO2) of single red blood cells within tumor microvessels (diameter: 3-12 micrometers) of DS-Carcinosarcoma was studied using a cryophotometric micromethod. In untreated control tumors (mean tissue temperature approx. 35 degrees C) the measured values scattered over the whole saturation r...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323754
更新日期:1982-01-01 00:00:00
abstract::The biological phenomena observed at low doses of ionizing radiation (adaptive response, bystander effects, genomic instability, etc.) are still not well understood. While at high irradiation doses, cellular death may be directly linked to DNA damage, at low doses, other cellular structures may be involved in what are...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-015-0622-5
更新日期:2016-03-01 00:00:00
abstract::Growth rates and cell diameters were determined from 12 species of plant roots exposed to a 60-Hertz (Hz) electric field of 360 Volts per meter (V/m) in an aqueous inorganic nutrient medium [conductivity: 0.07-0.09 Siemens per meter (S/m)]. The degree of growth depression ranged from zero to nearly 100 percent of cont...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01210937
更新日期:1985-01-01 00:00:00
abstract::Microcalorimetry, in combination with conventional methods for determining metabolic activity, opens the possibility to study the efficiency with which ATP, produced as a result of metabolic activity, is utilized by the cell for energy-requiring synthetic reactions. Using commercial baker's yeast as a test organism an...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323416
更新日期:1975-12-04 00:00:00
abstract::To explore possible applications of iodoacetate (IA), a glycolytic inhibitor, in cancer treatment, we screened its cytotoxicity and radioprotective/sensitizing efficacy in three different mammalian cell lines; A549 (human lung carcinoma), MCF7 (human mammary cancer), a non-cancerous CHO (Chinese hamster ovary) cells a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-017-0699-0
更新日期:2017-08-01 00:00:00
abstract::Total body Irradiation (TBI) is often used for conditioning, prior to bone marrow transplantation. Doses of 8-14 Gy in 1-8 fractions over 1-4 days are administered using low dose rate external beam radiotherapy (EBRT). When necessary, consolidation EBRT using conventional doses, fractionation and dose rate is given. T...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-009-0238-8
更新日期:2009-11-01 00:00:00
abstract::Simple, ellipsoidal geometries have long been the standard for estimating radiation dose rates in non-human biota (NHB). With the introduction of a regulatory protection standard that emphasizes protection of NHB as its own end point, there has been interest in improved models for the calculation of dose rates in NHB....
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-014-0546-5
更新日期:2014-08-01 00:00:00
abstract::An LBO (Li2B4O7) walled ionization chamber was designed to monitor the epithermal neutron fluence in boron neutron capture therapy clinical irradiation. The thermal and epithermal neutron sensitivities of the device were evaluated using accelerator neutrons from the 9Be(d, n) reaction at a deuteron energy of 4 MeV (4 ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-017-0700-y
更新日期:2017-08-01 00:00:00
abstract::A method which allows growth of normal human tissue to be studied in vitro is used to investigate possible interactive effects of radiation and environmentally important carcinogens on oesophageal and urothelial cell growth. Carcinogens chosen were selected for their known or suspected effect on the oesophageal mucosa...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211257
更新日期:1989-01-01 00:00:00
abstract::The fluorescent intercalation complex of ethidium bromide with DNA was used as a probe to demonstrate damage in the base-pair region of DNA, due to the action of superoxide radicals. The O.2- radical itself, generated by gamma-radiolysis of oxygenated aqueous Na-formate solutions, is rather ineffective with respect to...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01326732
更新日期:1984-01-01 00:00:00
abstract::Two data groups were analyzed: (1) the exposure rate in the former Czechoslovakia after the Chernobyl accident in 1986, and (2) the decrease of beta activity of an atmospheric fallout sample taken in Bratislava during 24 h on 30 May 1965. Both quantities decreased with the first power of time. This pattern of decrease...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-005-0276-9
更新日期:2005-05-01 00:00:00
abstract::The effect of supplying three different stable iodine levels (about 70, 80, and 160 mg day-1) in the daily feed of four dairy cows has been studied for the excretion pattern of 131I. None of the different treatments had a statistically significant effect. A mean milk transfer coefficient of 0.015 +/- 0.001 d l-1 was d...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01212680
更新日期:1994-01-01 00:00:00
abstract::This study examined the effects of three disasters (the Great East Japan Earthquake of March 11, 2011, followed by a tsunami and the Fukushima Daiichi Nuclear Power Plant accident) on feeding methods and growth in infants born after the disasters. Using results from the Fukushima Health Management Survey, Soso Distric...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-016-0636-7
更新日期:2016-05-01 00:00:00