Abstract:
:Activity catalysing double-strand DNA recombination has been investigated in human tumour cell lines using an in vitro assay in which nuclear extracts from tumour cells are used to catalyse homologous recombination between deletion plasmids. The cell lines investigated showed comparable constitutive levels of recombination activity. In several cell lines a two- fold to fourfold increase in the frequency of double-strand recombinational events catalysed by nuclear extracts was observed if the cells were exposed to low doses of ionizing radiation. The response was greatest for cells harvested at 6 h after radiation exposure, and the dose to produce an optimal effect was 25 cGy. Cell lines showing this response included a relatively radioresistant human colon cancer line and two cis-DDP (cis-diamminedichloroplatinum II) resistant ovarian tumour cell lines which are cross-resistant to radiation. Sub-lethal doses of cis-DDP were also effective in inducing upregulation of recombinational activity in the cis-DDP resistant cell lines. No change in recombinational activity was seen for radiation/drug-sensitive ovarian cell line following exposure to low drug or radiation doses. These findings are of particular interest since they involve a radiation-induced process with potential for direct involvement in DNA repair. Further studies will be aimed at determining if the extent of resistance to cytotoxic agents is causally related to the degree of inducible recombination activity.
journal_name
Radiat Environ Biophysjournal_title
Radiation and environmental biophysicsauthors
Lehnert S,Chow TYdoi
10.1007/s004110050056subject
Has Abstractpub_date
1997-02-01 00:00:00pages
67-70issue
1eissn
0301-634Xissn
1432-2099journal_volume
36pub_type
杂志文章abstract::Model experiments with two structurally different proteins (alcohol dehydrogenase and salmine) show that glycine, alanine, and tyrosine are by far more frequently involved in photochemically induced cross-link formations with DNA than is cysteine. The yields for cross-link formation of thymidine with salmine (cysteine...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01332171
更新日期:1976-07-30 00:00:00
abstract::Those working in interventional cardiology are exposed to varying radiation doses during diagnostic and interventional procedures. The work presented in this paper aimed to monitor the effective doses received by different categories of medical staff members practicing interventional cardiology procedures including se...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-020-00862-x
更新日期:2020-11-01 00:00:00
abstract::The probability of the formation of ionization clusters by primary alpha-particles at 5.4 MeV in nanometric volumes of propane was studied experimentally and by Monte Carlo simulation, as a function of the distance between the center line of the particle beam and the center of the target volume. The volumes were of cy...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-002-0171-6
更新日期:2002-12-01 00:00:00
abstract::C57Bl6-lacZ animals were exposed to a range of low dose-rate simulated solar particle event (sSPE) radiation at the NASA-sponsored Research Laboratory (NSRL) at Brookhaven National Laboratory (BNL). Peripheral blood was harvested from animals from 1 to 12 days after total body irradiation (TBI) to quantify the level o...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0291-3
更新日期:2010-08-01 00:00:00
abstract::Autoradiography is an effective tool for the imaging of radionuclide distributions in various samples. In sophisticated applications with special preparation and development of sample-emulsion combinations and subsequent grain counts it can be highly quantitative, but it requires carefully controlled conditions and a ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01209769
更新日期:1993-01-01 00:00:00
abstract::The evolution of the proflavine triplet state population with the extent of aggregation is examines, by ESRmeasurements, as a function of organic solvents and mineral salts, well known for their ability to influence the aggregation process. The intensity of the triplet state population has also been investigated as a ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323421
更新日期:1975-12-04 00:00:00
abstract::The number and type of stem cells in spleen and bone marrow of mice were determined after exposure to a single dose of 150 R on day 6, to a single dose of 500 R on day 6 or day 9 or to a fractionated dose of 150 R + 350 R on day 6 and 9. The stem cells were assayed on the basis of colony forming units (CFU) in spleen ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01324267
更新日期:1980-01-01 00:00:00
abstract::Four different thiol reagents: p-chloromercuribenzoic acid (pCMB), mercuric chloride (HgCl2), N-ethylmaleimide (NEM), and 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) were employed as agents modifying the transport of a hydrophilic and hydrophobic non-electrolyte spin labels: 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-o...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01323760
更新日期:1983-01-01 00:00:00
abstract::A temporary increase in the incidence of infant leukaemia in Greece was reported by Petridou et al., which was attributed to in utero exposure to ionising radiation resulting from the Chernobyl accident. We performed a similar analysis based on the data of the German Childhood Cancer Registry in order to check whether...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110050099
更新日期:1998-07-01 00:00:00
abstract::Quantitative cancer incidence data exist for various laboratory animal models, but little of this information is usable for estimating human risks, primarily because of uncertainties about possible mechanistic differences among species. Acceptance and utilization of animal data for human risk assessment will require a...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0106-3
更新日期:2007-06-01 00:00:00
abstract::Within the track structure code PARTRAC, DNA strand break induction by direct and indirect radiation action was calculated for the E. coli catabolite gene activator protein (CAP) DNA complex with (125)I located at the position of the H(5) atom of the cytosine near the center. The shape of the resulting DNA fragment si...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-004-0231-1
更新日期:2004-05-01 00:00:00
abstract::For studies on cell membranes, mice were exposed to mixed neutron + gamma reactor-radiation in the range of total doses from 0.5 to 4.5 Gy. Changes in functional activity of plasma membranes of erythrocytes, platelets, and lymphocytes were followed by a lectin-binding technique at various intervals afterwards. The amo...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01407598
更新日期:1984-01-01 00:00:00
abstract::The antioxidant properties of hydroxycinnamic acid derivatives (HCA) were studied by laser flash photolysis. The transient species with maximum absorption at 360 nm were assigned to the phenoxyl radical of HCA. The SO (4) (.-) induced oxidation of HCA was also investigated. It was shown that the interaction of SO (4) ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-006-0041-8
更新日期:2006-05-01 00:00:00
abstract::Various transport models are presently used to predict the long-term migration behaviour of fallout radiocesium on the soil. To examine to what extent the uncertainty of these predictions is influenced by the spatial variability of the migration rates, we determined the depth profiles of Chernobyl-derived 137Cs at 100...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110000062
更新日期:2000-09-01 00:00:00
abstract::The TEL1 gene from Saccharomyces cerevisiae has been shown to be the closest sequence homologue to ATM, the gene mutated in ataxia-telangiectasia (A-T) patients. Functional homology shared between the ATM and Tell proteins has recently been demonstrated based on heterologous expression of the TEL1 gene in human cells ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-001-0125-4
更新日期:2001-12-01 00:00:00
abstract::The potential of naturally occurring antioxidants to reduce the cellular oxidative damage induced by ionizing radiation has been studied for more than a decade for their pharmacological application during cancer treatment. It is already known that radioprotective efficacy of phytochemicals might influence various end ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-014-0551-8
更新日期:2014-11-01 00:00:00
abstract::It is shown by UV absorption and absolute fluorescence spectroscopy of solutions containing both DNA and quinacrine that the components experience mutual radio-protection due to scavenging of water radicals. From measurements at different ionic strengths it is inferred that quinacrine bound to DNA is more efficiently ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF02339804
更新日期:1975-06-13 00:00:00
abstract::The aim of this interdisciplinary project is to establish slice culture preparations from rodents and humans as a new model system for studying effects of X-rays and heavy ions within normal and tumor tissues. The advantage of such slice cultures relies on the conservation of an organotypic environment, the easy treat...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-010-0293-1
更新日期:2010-08-01 00:00:00
abstract::The migration of radionuclides from waste repositories to the biosphere potentially leads to a contamination of soil. Due to the importance of food production, the mobilisation and accumulation behaviour of long-lived radionuclides in the soil plays a key role in performance assessment studies. In this paper, the main...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章,评审
doi:10.1007/s004110100098
更新日期:2001-06-01 00:00:00
abstract::The biological phenomena observed at low doses of ionizing radiation (adaptive response, bystander effects, genomic instability, etc.) are still not well understood. While at high irradiation doses, cellular death may be directly linked to DNA damage, at low doses, other cellular structures may be involved in what are...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-015-0622-5
更新日期:2016-03-01 00:00:00
abstract::The use of Green's function has played a fundamental role in transport calculations for high-charge high-energy (HZE) ions. Two recent developments have greatly advanced the practical aspects of implementation of these methods. The first was the formulation of a closed-form solution as a multiple fragmentation perturb...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01211542
更新日期:1995-08-01 00:00:00
abstract::Within the Theory of Dual Radiation Action, the heuristically useful function, gamma(chi), that two energy depositions, a distance chi apart, will result in observable damage can be written in terms of two more fundamental quantities: One, s(chi), describes the structure of the sensitive matrix of the cell. The other,...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01210836
更新日期:1988-01-01 00:00:00
abstract::Growth rates and cell diameters were determined from 12 species of plant roots exposed to a 60-Hertz (Hz) electric field of 360 Volts per meter (V/m) in an aqueous inorganic nutrient medium [conductivity: 0.07-0.09 Siemens per meter (S/m)]. The degree of growth depression ranged from zero to nearly 100 percent of cont...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01210937
更新日期:1985-01-01 00:00:00
abstract::The rate constants for the photodynamic inactivation of hen egg-white lysozyme at different temperatures were studied. Arrhenius plots of the methylene blue sensitized photo-inactivation of lysozyme gave an experimental activation energy of 7.5 kcal/mol. The rate constants for the photodynamic inactivation of lysozyme...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01326898
更新日期:1979-02-23 00:00:00
abstract::The ion microprobe SNAKE at the Munich 14 MV tandem accelerator achieves beam focussing by a superconducting quadrupole doublet and can make use of a broad range of ions and ion energies, from 20 MeV protons to 200 MeV gold ions. Because of these properties, SNAKE is particularly attractive for biological microbeam ex...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-003-0222-7
更新日期:2004-02-01 00:00:00
abstract::Cl-36 is an important component of nuclear waste. The concentrations of stable chlorine (Cl) in pig and cow tissues were measured to provide information which can be used to parameterize models of (36)Cl transfer into agricultural animals. The concentration of stable Cl in cows' milk was 1.0 +/- 0.2 g L(-1), in cow mu...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-007-0137-9
更新日期:2008-02-01 00:00:00
abstract::With a model system of pBR322 plasmid DNA solution in vitro, the dose effects of radiation-induced single- and double-strand breaks (SSB and DSB) were measured and DSB was distinguished into alpha- and beta-types. Under the condition of low scavenging capacity existing in the irradiated DNA solution, SSB and alpha DSB...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s004110000054
更新日期:2000-06-01 00:00:00
abstract::Waterborne radioactive releases into the Techa River from the Mayak Production Association in Russia during 1949-1956 resulted in significant doses to about 30,000 persons who lived in downstream settlements. The residents were exposed to internal and external radiation. Two methods for reconstruction of the external ...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-015-0611-8
更新日期:2015-11-01 00:00:00
abstract::Responses of the S/S variant of the L5178Y murine leukemic lymphoblast, the photoreceptor cell of the rabbit retina and the lenticular epithelium of the rabbit to heavy ions (20Ne, 28Si, 40Ar and 56Fe) are described and discussed primarily from the standpoint of the need for a comprehensive theory of cellular radiosen...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/BF01209679
更新日期:1986-01-01 00:00:00
abstract::For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are relatively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tiss...
journal_title:Radiation and environmental biophysics
pub_type: 杂志文章
doi:10.1007/s00411-011-0371-z
更新日期:2011-08-01 00:00:00