Abstract：
:Endocytosis of cell surface receptors is an important regulatory event in signal transduction. The transforming growth factor beta (TGF-beta) superfamily signals to the Smad pathway through heteromeric Ser-Thr kinase receptors that are rapidly internalized and then downregulated in a ubiquitin-dependent manner. Here we demonstrate that TGF-beta receptors internalize into both caveolin- and EEA1-positive vesicles and reside in both lipid raft and non-raft membrane domains. Clathrin-dependent internalization into the EEA1-positive endosome, where the Smad2 anchor SARA is enriched, promotes TGF-beta signalling. In contrast, the lipid raft-caveolar internalization pathway contains the Smad7-Smurf2 bound receptor and is required for rapid receptor turnover. Thus, segregation of TGF-beta receptors into distinct endocytic compartments regulates Smad activation and receptor turnover.
journal_name
Nat Cell Bioljournal_title
Nature cell biologyauthors
Di Guglielmo GM,Le Roy C,Goodfellow AF,Wrana JLdoi
10.1038/ncb975keywords:
subject
Has Abstractpub_date
2003-05-01 00:00:00pages
410-21issue
5eissn
1465-7392issn
1476-4679pii
ncb975journal_volume
5pub_type
杂志文章abstract：:Clathrin-coated vesicles mediate endocytosis and transport between the trans-Golgi network (TGN) and endosomes in eukaryotic cells. Clathrin adaptors play central roles in coat assembly, interacting with clathrin, cargo and membranes. Two main types of clathrin adaptor act in TGN-endosome traffic: GGA proteins and the...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2427
更新日期：2012-02-19 00:00:00
abstract：:Autophagy is an evolutionarily conserved 'self-eating' process. Although the genes essential for autophagy (named Atg) have been identified in yeast, the molecular mechanism of how Atg proteins control autophagosome formation in mammalian cells remains to be elucidated. Here, we demonstrate that Bif-1 (also known as E...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1634
更新日期：2007-10-01 00:00:00
abstract：:Phosphoinositides (PtdIns) control fundamental cell processes, and inherited defects of PtdIns kinases or phosphatases cause severe human diseases, including Lowe syndrome due to mutations in OCRL, which encodes a PtdIns(4,5)P2 5-phosphatase. Here we unveil a lysosomal response to the arrival of autophagosomal cargo i...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3386
更新日期：2016-08-01 00:00:00
abstract：:Research on genome stability and integrity now extends far beyond the biochemistry of DNA repair to encompass signal transduction pathways that span numerous aspects of cellular life. Derailed genomic integrity pathways can result in debilitating genetic disorders, premature ageing, predisposition to cancer and degene...
journal_title：Nature cell biology
pub_type：
doi：10.1038/ncb1004-923
更新日期：2004-10-01 00:00:00
abstract：:Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short mult...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35023547
更新日期：2000-09-01 00:00:00
abstract：:The self-perpetuating amyloid isoform, or prion, of the yeast translation termination factor eRF3 modulates programmed translational frameshifting that controls a regulatory circuit determining the polyamine levels in a yeast cell. But it is still unclear whether this effect is adaptive or pathological. ...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb0908-1019
更新日期：2008-09-01 00:00:00
abstract：:Haematopoietic stem-progenitor cells (HSPCs) reside in the bone marrow niche, where interactions with osteoblasts provide essential cues for their proliferation and survival. Here, we use live-cell imaging to characterize both the site of contact between osteoblasts and haematopoietic progenitor cells (HPCs) and event...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1838
更新日期：2009-03-01 00:00:00
abstract：:Epithelial-mesenchymal transition (EMT) is a crucial embryonic programme that is executed by various EMT transcription factors (EMT-TFs) and is aberrantly activated in cancer and other diseases. However, the causal role of EMT and EMT-TFs in different disease processes, especially cancer and metastasis, continues to b...
journal_title：Nature cell biology
pub_type： 杂志文章,评审
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更新日期：2019-01-01 00:00:00
abstract：:The tumour-suppressor gene ATM, mutations in which cause the human genetic disease ataxia telangiectasia (A-T), encodes a key protein kinase that controls the cellular response to DNA double-strand breaks (DSBs). DNA DSBs caused by ionizing radiation or chemicals result in rapid ATM autophosphorylation, leading to che...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1651
更新日期：2007-11-01 00:00:00
abstract：:Aurora A, an integral mitotic kinase, is essential for microtubule dynamics of post-mitotic neurons. PKCzeta activates Aurora A, which in turn phosphorylates NDEL1 to promote neurite extension. This raises the possibility that Aurora A may also be involved in establishing cell polarity and axon/dendrite elaboration in...
journal_title：Nature cell biology
pub_type： 评论,新闻
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更新日期：2009-09-01 00:00:00
abstract：:The mechanistic target of rapamycin (mTOR) functions as a critical regulator of cellular growth and metabolism by forming multi-component, yet functionally distinct complexes mTORC1 and mTORC2. Although mTORC2 has been implicated in mTORC1 activation, little is known about how mTORC2 is regulated. Here we report that ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2860
更新日期：2013-11-01 00:00:00
abstract：:Formation of a bipolar spindle is essential for faithful chromosome segregation at mitosis. Because centrosomes define spindle poles, defects in centrosome number and structural organization can lead to a loss of bipolarity. In addition, microtubule-mediated pulling and pushing forces acting on centrosomes and chromos...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1474
更新日期：2006-10-01 00:00:00
abstract：:Many of the factors required for chromosomal DNA replication have been identified in unicellular eukaryotes. However, DNA replication is poorly understood in multicellular organisms. Here, we report the identification of GEMC1 (geminin coiled-coil containing protein 1), a novel vertebrate protein required for chromoso...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2050
更新日期：2010-05-01 00:00:00
abstract：:The Notch1 gene has an important role in mammalian cell-fate decision and tumorigenesis. Upstream control mechanisms for transcription of this gene are still poorly understood. In a chemical genetics screen for small molecule activators of Notch signalling, we identified epidermal growth factor receptor (EGFR) as a ke...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1750
更新日期：2008-08-01 00:00:00
abstract：:Organization of chromatin by epigenetic mechanisms is essential for establishing and maintaining cellular identity in developing and adult organisms. A key question that remains unresolved about this process is how epigenetic marks are transmitted to the next cell generation during cell division. Here we provide a mod...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1787
更新日期：2008-11-01 00:00:00
abstract：:Nutrient availability is central for T-cell functions and immune responses. Here we report that CD8+ T-cell activation and anti-tumour responses are strongly potentiated by the non-essential amino acid Asn. Increased Asn levels enhance CD8+ T-cell activation and effector functions against tumour cells in vitro and in ...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-020-00615-4
更新日期：2021-01-01 00:00:00
abstract：:A key step in nutrient sensing is activation of the master growth regulator, mTORC1 kinase, on the lysosomal membrane. Nutrients enable mTORC1 scaffolding by a complex composed of the Rag GTPases (Rags) and Ragulator, but the underlying mechanism of mTORC1 capture is poorly understood. Combining dynamic imaging in cel...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0148-6
更新日期：2018-09-01 00:00:00
abstract：:Cadherin-containing cell-cell junctions respond to intercellular tension by increasing their size, strength and complexity. The mechanical regulation of cadherin adhesions is now shown to involve myosin-dependent tension in the cortical actomyosin cytoskeleton. This reduces actin turnover to decrease the mobility of c...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb2980
更新日期：2014-06-01 00:00:00
abstract：:Hippo signalling has been associated with many important tissue functions including the regulation of organ size. In the intestinal epithelium differing functions have been proposed for the effectors of Hippo signalling, YAP and TAZ1. These are now shown to have a dual role in the intestinal epithelium, regulating bot...
journal_title：Nature cell biology
pub_type： 评论,杂志文章
doi：10.1038/ncb3086
更新日期：2015-01-01 00:00:00
abstract：:Extracellular signal-regulated kinase (ERK) controls fundamental cellular functions, including cell fate decisions. In PC12, cells shifting ERK activation from transient to sustained induces neuronal differentiation. As ERK associates with both regulators and effectors, we hypothesized that the mechanisms underlying t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1994
更新日期：2009-12-01 00:00:00
abstract：:Here we show that segregation of homologous chromosomes and that of sister chromatids are differentially regulated in Xenopus and possibly in other higher eukaryotes. Upon hormonal stimulation, Xenopus oocytes microinjected with antibodies against the anaphase-promoting complex (APC) activator Fizzy or the APC core su...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35050607
更新日期：2001-01-01 00:00:00
abstract：:Re-establishment of nuclear structure and chromatin organization after cell division is integral for genome regulation or development and is frequently altered during cancer progression. The mechanisms underlying chromatin expansion in daughter cells remain largely unclear. Here, we describe the transient formation of...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3641
更新日期：2017-12-01 00:00:00
abstract：:To learn more about cancer-associated fibroblasts (CAFs), we have isolated fibroblasts from different stages of breast cancer progression and analysed their function and gene expression. These analyses reveal that activation of the YAP transcription factor is a signature feature of CAFs. YAP function is required for C...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb2756
更新日期：2013-06-01 00:00:00
abstract：:Cancer-induced immune responses affect tumour progression and therapeutic response. In multiple murine models and clinical datasets, we identified large variations of neutrophils and macrophages that define 'immune subtypes' of triple-negative breast cancer (TNBC), including neutrophil-enriched (NES) and macrophage-en...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-019-0373-7
更新日期：2019-09-01 00:00:00
abstract：:Structural maintenance of chromosomes (SMC) protein complexes are key determinants of chromosome conformation. Using Hi-C and polymer modelling, we study how cohesin and condensin, two deeply conserved SMC complexes, organize chromosomes in the budding yeast Saccharomyces cerevisiae. The canonical role of cohesin is t...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3594
更新日期：2017-09-01 00:00:00
abstract：:The retinoblastoma protein (pRB) negatively regulates the progression from G1 to S phase of the cell cycle, in part, by repressing E2F-dependent transcription. pRB also possesses E2F-independent functions that contribute to cell-cycle control--for example, during pRB-mediated cell-cycle arrest pRB associates with Skp2...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb1532
更新日期：2007-02-01 00:00:00
abstract：:Despite advances in the differentiation of insulin-producing cells from human embryonic stem cells, the generation of mature functional β cells in vitro has remained elusive. To accomplish this goal, we have developed cell culture conditions to closely mimic events occurring during pancreatic islet organogenesis and β...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/s41556-018-0271-4
更新日期：2019-02-01 00:00:00
abstract：:Accurate chromosome segregation during cell division in metazoans relies on proper chromosome congression at the equator. Chromosome congression is achieved after bi-orientation to both spindle poles shortly after nuclear envelope breakdown, or by the coordinated action of motor proteins that slide misaligned chromoso...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb3060
更新日期：2014-12-01 00:00:00
abstract：:The Drosophila fruitless (fru) gene product Fru has been postulated to be a neural sex-determination factor that directs the development of at least two male-specific characteristics, namely courtship behaviour and formation of the muscle of Lawrence (MOL). The fru gene encodes a putative transcription factor with a B...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/35019537
更新日期：2000-08-01 00:00:00
abstract：:Endoplasmic reticulum (ER)-associated protein degradation by the ubiquitin-proteasome system requires the dislocation of substrates from the ER into the cytosol. It has been speculated that a functional ubiquitin proteasome pathway is not only essential for proteolysis, but also for the preceding export step. Here, we...
journal_title：Nature cell biology
pub_type： 杂志文章
doi：10.1038/ncb746
更新日期：2002-02-01 00:00:00
